摘" 要:豇豆蚜、二斑葉螨、豆大薊馬是豇豆苗期的重要害蟲(chóng),嚴(yán)重影響?hù)姑缙谏L(zhǎng)發(fā)育。為明確4種種衣劑10%噻蟲(chóng)胺種子處理懸浮劑(FS)、30%噻蟲(chóng)嗪FS、600 g/L吡蟲(chóng)啉懸浮種衣劑(FSC)、8%氟蟲(chóng)腈FSC對(duì)豇豆的安全性及對(duì)3種豇豆苗期害蟲(chóng)的防控作用,本研究通過(guò)室內(nèi)盆栽接蟲(chóng)試驗(yàn),研究4種衣劑對(duì)豇豆出苗的影響及對(duì)豇豆蚜、二斑葉螨、豆大薊馬的防治效果。結(jié)果表明:10%噻蟲(chóng)胺FS、30%噻蟲(chóng)嗪FS、600 g/L吡蟲(chóng)啉FSC、8%氟蟲(chóng)腈FSC處理的豇豆發(fā)芽率分別為95.83%、91.67%、95.83%、95.83%,略低于對(duì)照的發(fā)芽率(100%),但對(duì)豇豆生長(zhǎng)無(wú)明顯影響。30%噻蟲(chóng)嗪FS對(duì)豇豆蚜和豆大薊馬的防效均最好,接蟲(chóng)3、7、14 d后對(duì)豇豆蚜的防效分別為65.04%、81.10%和72.35%,對(duì)豆大薊馬的防效分別為15.41%、58.61%和54.89%;隨著接蟲(chóng)時(shí)間的推遲,種衣劑處理對(duì)豇豆蚜、豆大薊馬的防治效果有所下降;4種種衣劑包衣處理對(duì)二斑葉螨的防治效果均不顯著。研究結(jié)果為生產(chǎn)上豇豆苗期害蟲(chóng)的防控提供技術(shù)支撐。
關(guān)鍵詞:種衣劑;豇豆;害蟲(chóng);防控效果;室內(nèi)研究中圖分類(lèi)號(hào):S436.43 """""文獻(xiàn)標(biāo)志碼:A
Indoor Control Efficacy of Four Seed Coating Treatments Against Three Common Pests of Cowpea Seedlings
LIU Ying1,2, LIANG Xiao1,2, WU Chunling1,2, AN Xingkui1,2, LI Tiantian1,2, CHNE Qing1,2*
1. Institute of Environment and Plant Protection, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture and Rural Affairs / Hainan Engineering Research Center for Biological Control of Tropical Crops Diseases and Insect Pests / Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests, Haikou, Hainan 571101, China; 2. Sanya Research Academy, Chinese Academy of Tropical Agriculture Sciences / Hainan Key Laboratory for Biosafety Monitoring and Molecular Breeding in Off-season Reproduction Regions, Sanya, Hainan 572000, China
Abstract: Cowpea aphids, two-spotted spider mites, and bean thrips are main pests during the cowpea seedling stage, severely impacting the growth and development of cowpea plants. To evaluate the safety and control efficacy of four seed coating treatments, 10% clothianidin flowable concentrate for seed treatment FS, 30% thiamethoxan FS, 600 g/L imidacloprid flowable concentrate for seed coating FSC and 8% fipronil FSC on three common pests during the cowpea seedling stage, this study conducted indoor pot experiments with insect infestations. The experiments aimed to evaluate the impact of the four seed coatings on cowpea emergence and the effectiveness in controlling cowpea aphids, two-spotted spider mites, and bean thrips. The results indicated that the germination rate of cowpea treated with 10% clothianidin FS, 30% thiamethoxan FS, 600 g/L imidacloprid FSC, and 8% fipronil FSC was 95.83%, 91.67%, 95.83% and 95.83%, respectively. The rate was slightly lower than that of the control (100%), but did not significantly affect crop growth. The findings revealed that 30% thiamethoxan FS exhibited the highest control efficacy against both cowpea aphids and bean thrips. Specifically, the control efficacy against cowpea aphids at 3, 7 and 14 days post-infestation was 65.04%, 81.10% and 72.35%, respectively. For bean thrips, the control efficacy at the same time points was 15.41%, 58.61% and 54.89%, respectively. As the infestation period progressed, the effectiveness of the seed coating treatments in controlling cowpea aphids and bean thrips gradually decreased. None of the four seed coating treatments demonstrated significant control efficacy against two-spotted spider mites. This research provides technical support for pest management during the seedling stage of cowpea production.
Keywords: seed coating formulations; cowpea; pest; control efficacy; laboratory study
DOI: 10.3969/j.issn.1000-2561.2025.07.023
豇豆[Vigna unguiculata (L.) Walp.]是我國(guó)重要的豆類(lèi)蔬菜作物之一,在我國(guó)南北方廣泛種植,2021年全國(guó)豇豆總產(chǎn)量達(dá)1490萬(wàn)t,人均年消費(fèi)量超過(guò)10 kg[1]。近年來(lái),海南豇豆種植面積穩(wěn)定在2萬(wàn)hm2以上,年產(chǎn)量近60萬(wàn)t,僅次于辣椒,是海南冬季瓜菜的主要種植品種,也是當(dāng)?shù)剞r(nóng)民的重要收入來(lái)源[2]。豇豆蚜(Aphis craccivora Koch)、二斑葉螨(Tetranychus urticae Koch)、豆大薊馬(Megalurothrips usitatus Bagnall)是豇豆苗期的3種重要害蟲(chóng),由于其體型小、繁殖能力強(qiáng)、隱蔽性強(qiáng)等特點(diǎn),嚴(yán)重影響?hù)姑缙谏L(zhǎng)發(fā)育。目前對(duì)豇豆蚜、二斑葉螨、豆大薊馬的防治主要依賴(lài)化學(xué)農(nóng)藥,而長(zhǎng)期使用化學(xué)農(nóng)藥面臨環(huán)境污染、害蟲(chóng)抗藥性不斷升高等問(wèn)題,且用藥不規(guī)范、不科學(xué)導(dǎo)致的農(nóng)藥殘留問(wèn)題日趨嚴(yán)重。2021年5月31日,農(nóng)業(yè)農(nóng)村部印發(fā)《食用農(nóng)產(chǎn)品“治違禁、控藥殘、促提升”三年行動(dòng)方案》,豇豆作為“三棵菜”(豇豆、韭菜、芹菜)之一被列入重點(diǎn)治理清單,豇豆質(zhì)量安全問(wèn)題受到廣泛關(guān)注[3],因此尋求豇豆苗期害蟲(chóng)的綠色防控方法成為亟待解決的生產(chǎn)需求。與傳統(tǒng)施藥方式相比,種子包衣施藥技術(shù)是一種“定點(diǎn)施藥”方式,
具有給藥精準(zhǔn)、釋放緩慢、藥效期長(zhǎng)和天敵昆蟲(chóng)安全等特點(diǎn),同時(shí)還具有省時(shí)省力、節(jié)約勞動(dòng)成本等優(yōu)點(diǎn),能實(shí)現(xiàn)提高防治效果、減少化學(xué)農(nóng)藥使用量的目標(biāo)[4-5]。種衣劑包衣處理防治病蟲(chóng)害作為一種最經(jīng)濟(jì)有效的方法,已在我國(guó)大宗糧食作物和經(jīng)濟(jì)作物生產(chǎn)中廣泛應(yīng)用,并且取得了頗為顯著的經(jīng)濟(jì)、社會(huì)及生態(tài)效益[6-10]。我國(guó)農(nóng)藥登記中常用于種衣劑的殺蟲(chóng)劑主要有噻蟲(chóng)嗪、吡蟲(chóng)啉、噻蟲(chóng)胺和氟蟲(chóng)腈等內(nèi)吸性農(nóng)藥,多用于防治蚜蟲(chóng)、飛虱、薊馬、金針蟲(chóng)和蠐螬等害蟲(chóng),當(dāng)前應(yīng)用種衣劑防治豇豆苗期害蟲(chóng)的藥劑尚無(wú)相關(guān)產(chǎn)品登記,也鮮有相關(guān)研究報(bào)道。本研究選用4種市場(chǎng)常見(jiàn)種衣劑10%噻蟲(chóng)胺FS、30%噻蟲(chóng)嗪FS、600"g/L吡蟲(chóng)啉FSC、8%氟蟲(chóng)腈FSC,采取室內(nèi)盆栽接蟲(chóng)試驗(yàn),評(píng)價(jià)其對(duì)豇豆蚜、二斑葉螨、豆大薊馬的防治效果及對(duì)豇豆出苗、生長(zhǎng)的安全性,以期為生產(chǎn)上豇豆苗期害蟲(chóng)的防控提供技術(shù)支撐。
1.1" 材料
1.1.1" 供試種衣劑" 供試種衣劑信息見(jiàn)表1。
1.1.2" 供試蟲(chóng)源nbsp; 豇豆蚜(Aphis craccivora Koch)、二斑葉螨(Tetranychus urticae Koch)、豆大薊馬(Megalurothrips usitatus Bagnall))均為本實(shí)驗(yàn)室養(yǎng)殖繁育多代的蟲(chóng)源,其中豇豆蚜、二斑葉螨均用豇豆苗飼養(yǎng),豆大薊馬用油豆角豆莢飼養(yǎng)。養(yǎng)蟲(chóng)室條件:溫度為(26±2)℃,12(光照)∶12(黑暗)。
1.2" 方法
1.2.1" 拌種及苗期安全性測(cè)試" 用移液槍移取10%噻蟲(chóng)胺FS 120"μL、30%噻蟲(chóng)嗪FS 60"μL、600"g/L吡蟲(chóng)啉FSC 60"μL、8%氟蟲(chóng)腈FSC 40 μL,分別加入0.50"mL去離子水調(diào)成漿狀藥液,然后將藥液倒入飽滿(mǎn)、均一的4份豇豆種子(每份50粒,約10"g)中,充分?jǐn)嚢?,待均勻著藥后,于通風(fēng)陰涼處攤開(kāi)晾干。設(shè)置清水處理作為對(duì)照。將處理后的種子播入盛有約30 g基質(zhì)(山東綠珠農(nóng)業(yè)科技發(fā)展有限公司,有機(jī)質(zhì)含量gt;30%)的塑料盆中(長(zhǎng)×寬×高為5"cm×4.5"cm×6"cm),每盆播種1粒豇豆種子,置于光照溫室內(nèi)進(jìn)行統(tǒng)一管理,每隔4~5"d澆水30 mL,播種14 d后統(tǒng)計(jì)發(fā)芽數(shù),計(jì)算發(fā)芽率,測(cè)定株高、鮮質(zhì)量和葉綠素(SPAD-502Plus便攜式葉綠素儀)相對(duì)含量。
1.2.2" 防效試驗(yàn)" 播種14 d后,每處理(4個(gè)藥劑處理+對(duì)照)選取生長(zhǎng)發(fā)育一致(五葉一心)的豇豆幼苗30盆,即每種害蟲(chóng)接種10盆豇豆。接蟲(chóng)時(shí),將每盆豇豆苗置于養(yǎng)蟲(chóng)籠中(長(zhǎng)×寬×高為28 cm×28 cm×50 cm)后分別接入豇豆蚜、二斑葉螨和豆大薊馬,其中每株接種10頭豇豆蚜成蟲(chóng),每株接種10頭二斑葉螨雌成螨,每籠接種20頭豆大薊馬雌成蟲(chóng)。接蟲(chóng)后3、7、14 d調(diào)查每株或每籠的害蟲(chóng)數(shù)量,調(diào)查所有成蟲(chóng)和幼蟲(chóng),不含卵,共調(diào)查3次,按照公式計(jì)算蟲(chóng)口減退率和防治效果。蟲(chóng)口減退率=(接入蟲(chóng)口基數(shù)-接蟲(chóng)后n d活蟲(chóng)數(shù))/接入蟲(chóng)口基數(shù)×100%;校正防效=(種衣劑處理組蟲(chóng)口減退率-對(duì)照蟲(chóng)口減退率)/(100-對(duì)照蟲(chóng)口減退率)×100%。
1.3 "數(shù)據(jù)處理
采用Excel 2019軟件進(jìn)行數(shù)據(jù)處理,采用Graphpad prism 9.5軟件進(jìn)行繪圖。使用DPS軟件(V7.05-V9.01)采用單因素方差(Tukey檢驗(yàn)法)進(jìn)行不同處理間差異分析。
2.1" 4種種衣劑處理對(duì)豇豆出苗、生長(zhǎng)的影響
由圖1A可知,10%噻蟲(chóng)胺FS、30%噻蟲(chóng)嗪FS、600 g/L吡蟲(chóng)啉FSC、8%氟蟲(chóng)腈FSC處理后豇豆的發(fā)芽率分別為95.83%、91.67%、95.83%、95.83%,發(fā)芽率均在90%以上,低于CK發(fā)芽率(100%),表明種衣劑處理對(duì)豇豆發(fā)芽率存在一定影響。播種14 d后,4種種衣劑處理的豇豆苗株高分別為31.18、29.88、36.19、35.19"cm,其中吡蟲(chóng)啉處理的苗株高顯著高于CK,噻蟲(chóng)胺和噻蟲(chóng)嗪處理略低于CK,但與CK無(wú)顯著性差異(圖1B)。4種種衣劑處理豇豆苗的鮮質(zhì)量與CK無(wú)顯著性差異(圖1C)。種衣劑處理的豇豆苗根長(zhǎng)表現(xiàn)出相同的趨勢(shì),4種種衣劑處理的根長(zhǎng)分別為7.85、7.81、8.33、8.38 cm,而CK的根長(zhǎng)為7.96"cm,4種處理與CK無(wú)顯著性差異(圖1D)。4種處理的葉綠素相對(duì)含量與CK無(wú)顯著性差異(圖1E)。
2.2" 4種種衣劑處理對(duì)豇豆蚜的防效
由表2可知,接蟲(chóng)后3 d調(diào)查發(fā)現(xiàn),10%噻蟲(chóng)胺FS、30%噻蟲(chóng)嗪FS、600 g/L吡蟲(chóng)啉FSC、8%氟蟲(chóng)腈FSC處理組均對(duì)豇豆蚜有一定的防治效果,噻蟲(chóng)嗪、吡蟲(chóng)啉和氟蟲(chóng)腈處理的防效分別為65.04%、62.95%和60.24%,顯著高于噻蟲(chóng)胺處理的防效(12.39%);接蟲(chóng)后7"d,噻蟲(chóng)嗪、吡蟲(chóng)啉
和氟蟲(chóng)腈的防效進(jìn)一步提高分別達(dá)到81.10%、79.02%和74.56%;接蟲(chóng)后14 d,噻蟲(chóng)嗪、吡蟲(chóng)啉和氟蟲(chóng)腈的防效有所下降,分別為72.35%、70.81%和66.53%。綜上,噻蟲(chóng)嗪、吡蟲(chóng)啉和氟蟲(chóng)腈種衣劑包衣均對(duì)豇豆蚜有較好的防效,其中噻蟲(chóng)嗪的防效最好,噻蟲(chóng)胺防效最差。4種種衣劑處理對(duì)豇豆蚜14 d的防治效果見(jiàn)圖2。
2.3 "4種種衣劑處理對(duì)二斑葉螨的防效
接蟲(chóng)后3 d由于二斑葉螨的數(shù)量未發(fā)生變化,因此未計(jì)算其校正防效,其原因是二斑葉螨產(chǎn)的卵還未孵化為幼螨。由表3可知,接蟲(chóng)后7 d調(diào)查發(fā)現(xiàn),10%噻蟲(chóng)胺FS、30%噻蟲(chóng)嗪FS、600 g/L吡蟲(chóng)啉FSC、8%氟蟲(chóng)腈FSC處理對(duì)二斑葉螨的防治效果較差,噻蟲(chóng)胺、噻蟲(chóng)嗪、吡蟲(chóng)啉和氟蟲(chóng)腈處理的防效分別為8.26%、7.25%、4.17%和4.68%;接蟲(chóng)后14 d,噻蟲(chóng)胺、噻蟲(chóng)嗪、吡蟲(chóng)啉和氟蟲(chóng)腈處理的防效分別為6.41%、1.46%、5.59%和1.43%。綜上,噻蟲(chóng)胺、噻蟲(chóng)嗪、吡蟲(chóng)啉和氟蟲(chóng)腈種衣劑包衣對(duì)豇豆苗期二斑葉螨基本均無(wú)防治效果。4種種衣劑處理對(duì)二斑葉螨14 d的防治效果見(jiàn)圖3。
2.4" 4種種衣劑處理對(duì)豆大薊馬的防效
由表4可知,接蟲(chóng)后3 d調(diào)查發(fā)現(xiàn),10%噻蟲(chóng)胺FS、30%噻蟲(chóng)嗪FS、600 g/L升吡蟲(chóng)啉FSC、8%氟蟲(chóng)腈FSC處理均對(duì)豆大薊馬防效較低,噻蟲(chóng)胺、噻蟲(chóng)嗪、吡蟲(chóng)啉和氟蟲(chóng)腈處理組的防效分別為10.35%、15.41%、1.93%和0.57%;接蟲(chóng)后7 d,4種種衣劑處理組的防效有所提高,分別達(dá)到50.64%、58.61%、38.13%和29.23%,其中噻蟲(chóng)嗪和噻蟲(chóng)胺的防效無(wú)顯著性差異,而噻蟲(chóng)嗪的防效顯著高于吡蟲(chóng)啉和氟蟲(chóng)腈;接蟲(chóng)后14 d,4種種衣劑處理的防效有所下降,分別為48.32%、54.89%、30.96%和23.25%。綜上,噻蟲(chóng)嗪處理對(duì)豆大薊馬的防效最好,氟蟲(chóng)腈的防效最差。4種種衣劑處理對(duì)豆大薊馬14 d的防治效果見(jiàn)圖4。
本研究室內(nèi)評(píng)估了市面常見(jiàn)的4種種衣劑10%噻蟲(chóng)胺FS、30%噻蟲(chóng)嗪FS、600"g/L吡蟲(chóng)啉FSC、8%氟蟲(chóng)腈FSC對(duì)3種豇豆苗期害蟲(chóng)的防控作用,其中30%噻蟲(chóng)嗪FS對(duì)豇豆蚜和豆大薊馬的防效均最好,推測(cè)與噻蟲(chóng)嗪在試驗(yàn)劑量下豇豆苗可高效內(nèi)吸傳導(dǎo)至地上部分對(duì)豇豆蚜和豆大薊馬發(fā)揮藥效有關(guān)。陸晴等[11]也研究發(fā)現(xiàn),22.6%噻蟲(chóng)嗪FS對(duì)小麥蚜蟲(chóng)的防效高于18%噻蟲(chóng)胺FS和60%吡蟲(chóng)啉FSC。噻蟲(chóng)嗪是第2代新煙堿類(lèi)殺蟲(chóng)劑,具有殺蟲(chóng)譜廣、內(nèi)吸性好、持效期長(zhǎng)的優(yōu)點(diǎn),可用于葉面噴霧、種子和土壤處理[12]。目前已登記的包含噻蟲(chóng)嗪成分防治蚜蟲(chóng)的種衣劑達(dá)45種,防治薊馬的種衣劑有30種(中國(guó)農(nóng)藥信息網(wǎng)),可見(jiàn)噻蟲(chóng)嗪種子包衣處理對(duì)2種害蟲(chóng)的防治潛力。本研究結(jié)果表明,4種種衣劑處理對(duì)二斑葉螨基本無(wú)防治效果,與其殺螨活性較差有關(guān),后續(xù)可以研究具有內(nèi)吸活性的殺螨劑,如蟲(chóng)螨腈、丁醚脲等種子處理對(duì)二斑葉螨的防控效果。
本研究發(fā)現(xiàn)10%噻蟲(chóng)胺FS、30%噻蟲(chóng)嗪FS、600 g/L吡蟲(chóng)啉FSC、8%氟蟲(chóng)腈FSC處理的豇豆發(fā)芽率分別為95.83%、91.67%、95.83%、95.83%,低于CK發(fā)芽率(100%),同時(shí)噻蟲(chóng)胺、噻蟲(chóng)嗪處理的株高和根長(zhǎng)略低于CK。種衣劑的安全性是利用該技術(shù)進(jìn)行苗期病蟲(chóng)害防控的重點(diǎn)關(guān)注的問(wèn)題。魏晨等[13]研究表明70%吡蟲(chóng)啉水分散粒劑的用量高于5 g/kg不僅明顯抑制玉米種子萌發(fā),還使根系活力和葉綠素含量顯著下降,且葉部出現(xiàn)藥害癥狀;房鋒等[14]研究發(fā)現(xiàn)15%克百威·福美雙·三唑酮種衣劑處理后,玉米幼苗的株高、初根長(zhǎng)和鮮質(zhì)量明顯受到抑制。種衣劑的安全性與以下因素有關(guān):(1)種衣劑由殺蟲(chóng)劑、殺菌劑、驅(qū)鼠劑、復(fù)合肥、微量元素、植物生長(zhǎng)調(diào)節(jié)劑、增效劑、緩釋劑、成膜劑及助劑(分散劑、懸浮劑、表面活性劑等)制備而成[15],其中各組分因劑量配比、功能疊加以及配伍關(guān)系均可能引發(fā)藥害;(2)溫度等環(huán)境原因造成的種衣劑安全性問(wèn)題,如低溫引起種苗代謝停止,藥劑不能快速排出體外而中毒;(3)農(nóng)藝措施造成的種衣劑安全性問(wèn)題,包括拌種溫度、播種深度、拌種與播種間隔時(shí)間等[16]。因此,造成種衣劑副作用的因素很多,大田使用前應(yīng)綜合考慮拌種劑量、區(qū)域性氣候差異和農(nóng)藝措施對(duì)種衣劑安全性的潛在影響,以免造成不必要的損失。
種衣劑的防治效果與使用劑量相關(guān),黃紅霞等[17]使用3個(gè)劑量(150、250、350"mL/100"kg種子)的246 g/L吡蟲(chóng)啉·戊唑醇FS防治水稻薊馬的研究表明,350 mL/100 kg種子處理對(duì)薊馬的整體防治效果最好;王猛[18]也得出相似的研究結(jié)果,48.00 g/100 kg種子的12%苯醚甲環(huán)唑·氟蟲(chóng)腈FS對(duì)玉米絲黑穗病的防效高于36.00、42.00"g/100 kg種子2個(gè)劑量處理。在使用劑量不變的情況下,種衣劑的防治效果與種子的包衣藥量有關(guān),本研究拌種時(shí)發(fā)現(xiàn),由于豇豆種子表皮光滑,包衣時(shí)包被到種子表面的藥劑較少,多數(shù)被滯留在拌種器皿表面,因此應(yīng)研發(fā)與豇豆種子匹配的成膜劑以增加種衣劑在豇豆種子表面的負(fù)載量。
當(dāng)前種衣劑的研發(fā)以?xún)稍獜?fù)配和三元復(fù)配為主[19-20],達(dá)到作物苗期病蟲(chóng)害的多重防治的目的。本研究結(jié)果表明,噻蟲(chóng)嗪對(duì)豇豆蚜和豆大薊馬的防效較好,后續(xù)可以與篩選出的防治豇豆二斑葉螨、斑潛蠅的藥劑或者豇豆苗期的重要病害枯萎病的藥劑進(jìn)行復(fù)配。同時(shí),本研究結(jié)果僅在室內(nèi)進(jìn)行,田間防效還有待進(jìn)一步驗(yàn)證。
參考文獻(xiàn)