低能量強(qiáng)脈沖光影響C57BL/6小鼠毛發(fā)生長(zhǎng)的實(shí)驗(yàn)研究

江樂文　丁偉佳　江敏君

【摘要】 目的：觀察低能量強(qiáng)脈沖光（IPL）對(duì)C57BL/6小鼠毛發(fā)生長(zhǎng)及生長(zhǎng)周期的影響。方法：制作小鼠休止期脫毛模型，將35只小鼠隨機(jī)分為陽(yáng)性對(duì)照組、空白對(duì)照組、IPL高能量組、IPL中能量組、IPL低能量組，對(duì)IPL高能量組、IPL中能量組、IPL低能量組脫毛區(qū)進(jìn)行IPL照射，比較各組小鼠毛發(fā)生長(zhǎng)情況。實(shí)驗(yàn)14、21 d，取各組鼠背脫毛區(qū)皮膚行HE染色及毛囊計(jì)數(shù)，觀察IPL照射對(duì)毛囊生長(zhǎng)的影響。結(jié)果：IPL低能量組毛發(fā)長(zhǎng)度顯著長(zhǎng)于其他組，每倍視野毛囊數(shù)為（68.9±5.4）個(gè)，明顯多于其他組，差異均有統(tǒng)計(jì)學(xué)意義（P<0.05）。結(jié)論：低能量IPL照射可能是促進(jìn)毛發(fā)生長(zhǎng)的一種有效治療手段。

【關(guān)鍵詞】 強(qiáng)脈沖光 休止期 毛發(fā)生長(zhǎng) 生長(zhǎng)周期 毛囊

doi：10.14033/j.cnki.cfmr.2020.18.005 文獻(xiàn)標(biāo)識(shí)碼 A 文章編號(hào) 1674-6805（2020）18-00-03

Effects of Low Energy Intense Pulse Light on Hair Growth in C57BL/6 Mice/JIANG Lewen， DING Weijia， JIANG Minjun. //Chinese and Foreign Medical Research， 2020， 18（18）： -12

[Abstract] Objective： To observe the effects of low energy intense pulse light （IPL） on hair growth and growth cycle in C57BL/6 mice. Method： The depilation model of telogen was made， thirty-five mice were randomly divided into the positive control group， the blank control group， the IPL high energy group， the IPL medium energy group and the IPL low energy group. IPL irradiation was performed on the depilation area of the IPL high energy group， the IPL medium energy group and IPL low energy group. Hair growth of mice in each group was compared. On day 14 and 21， HE staining and hair follicles counting were performed on the depilated area of each group， and the effect of IPL irradiation on hair follicles growth was observed. Result： The length of the hair in IPL low energy group was significantly longer than those of the other groups， and the number of follicles per visual field was （68.9±5.4）， which was significantly more than those of the other groups， and the differences were statistically significant （P<0.05）. Conclusion： Low intensity IPL irradiation may be an effective treatment for hair growth.

[Key words] Intense pulse light Telogen Hair growth Growth cycle Hair follicles

First-authors address： Shenzhen Longgang Centra Hospital， Shenzhen 518116， China

光電治療在皮膚美容中的應(yīng)用越來(lái)越廣泛。強(qiáng)脈沖光（intense pulse light，IPL）已被廣泛用于脫毛治療中，主要原理為選擇性光熱效應(yīng)作用于富含黑色素顆粒細(xì)胞的毛囊、毛干，導(dǎo)致毛囊組織破壞，從而達(dá)到脫毛效果。反常多毛現(xiàn)象（paradoxical hypertrichosis）是強(qiáng)脈沖光治療后的副作用，于2002年首先被發(fā)現(xiàn)并被證實(shí)[1]。反常多毛現(xiàn)象表現(xiàn)為在脫毛區(qū)域或鄰近區(qū)域出現(xiàn)毛發(fā)的反常增多增粗，因此存在潛在治療脫發(fā)的可能。本課題將在C57BL/6小鼠動(dòng)物實(shí)驗(yàn)中驗(yàn)證低能量強(qiáng)脈沖光對(duì)毛發(fā)生長(zhǎng)的影響，可能有助于開發(fā)一種操作便捷、費(fèi)用低廉的脫發(fā)治療新手段。

1 材料與方法

1.1 一般材料

1.1.1 實(shí)驗(yàn)動(dòng)物 SPF級(jí)雄性C57BL/6小鼠，周齡6～7周，體重15～20 g，購(gòu)自南方醫(yī)科大學(xué)實(shí)驗(yàn)動(dòng)物中心。

1.1.2 實(shí)驗(yàn)試劑 5%米諾地爾酊（廈門美商醫(yī)藥有限公司，國(guó)藥準(zhǔn)字H20103166）;硫化鈉、石蠟、甲醛、酒精、二甲苯、伊紅染液、蘇木精染液，均市購(gòu)。

1.1.3 實(shí)驗(yàn)儀器 強(qiáng)脈沖光治療儀（SOLARI路創(chuàng)麗，韓國(guó)）、CMl900切片機(jī)、顯微鏡等。

1.2 方法

1.2.1 動(dòng)物實(shí)驗(yàn) 將小鼠放于溫度為25 ℃，濕度為54%的環(huán)境中適應(yīng)性喂養(yǎng)2周后，采用電動(dòng)剃毛器剃除各組小鼠背部毛發(fā)，面積約2 cm×3 cm，再用8%硫化鈉脫毛濕布擦拭，保證毛發(fā)脫干凈及皮膚無(wú)損傷。采用抽簽法將35只小鼠隨機(jī)分為5組，分別為IPL高能量組、IPL中能量組、IPL低能量組、空白對(duì)照組和陽(yáng)性對(duì)照組，每組7只，分籠飼養(yǎng)。脫毛次日開始進(jìn)行實(shí)驗(yàn)干預(yù)，各組干預(yù)方法：IPL低能量組參數(shù)：波長(zhǎng)560～

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（收稿日期：2020-03-26） （本文編輯：李盈）