輔酶Q10霜劑對紫外線損傷小鼠血細(xì)胞及抗氧化酶的影響

吳海游+邱楚群+胡紫薇+鄭靜彬+黃志榮+呂思敏+吳鐵

[摘要] 目的 探討輔酶Q10霜劑對紫外線損傷小鼠血細(xì)胞及抗氧化酶的影響。 方法 36只昆明小鼠背部皮膚進行脫毛后，采用體重因素隨機分成4組：正常組（CON）、模型組（MOL）、輔酶組（CoQ）、陽性組（POS）。其中CON不做任何處理；MOL、CoQ、POS組小鼠背部皮膚分別涂抹空白霜劑、輔酶Q10霜劑、二苯甲酮霜劑，涂抹霜劑30 min后進行紫外線照射。每天1次，持續(xù)8周。結(jié)束后，小鼠眼球取血處死，對血樣進行指標(biāo)檢測。 結(jié)果 與CON比較，MOL小鼠白細(xì)胞（WBC）數(shù)量升高（P < 0.05），丙二醛（MDA）含量增高（P < 0.05），血漿超氧化物歧化酶（SOD）、谷胱甘肽過氧化物酶（GSH-Px）活力減弱（P < 0.05）；與MOL比較，CoQ小鼠WBC數(shù)量減少（P < 0.05），MDA含量降低（P < 0.05），SOD、GSH-Px活力增強（P < 0.05）；與MOL比較，POS小鼠WBC數(shù)量減少（P < 0.05），MDA含量降低（P < 0.05），SOD、GSH-Px活力增強（P < 0.05）。 結(jié)論 輔酶Q10霜劑對紫外線損傷小鼠具有提升血漿SOD、GSH-Px活力與降低MDA含量的能力，其有助于減緩對紫外線損傷小鼠的應(yīng)激狀態(tài)。

[關(guān)鍵詞] 輔酶Q10；紫外線；血細(xì)胞；氧化因子

[中圖分類號] R75 [文獻標(biāo)識碼] A [文章編號] 1673-7210（2017）03（a）-0028-04

The influence of co-enzyme Q10 Cream on blood cell and antioxidant enzymes withiin the mice exposed on ultraviolet ray

WU Haiyou1 QIU Chuqun1 HU Ziwei1 ZHENG Jingbin1 HUANG Zhirong2 LU Simin3 WU Tie1，3

1.School of Pharmacy， Guangdong Medical University， Guangdong Province， Dongguan 523808， China； 2.Pharmaceutical Department， the Third People's Hospital of Dongguan City， Guangdong Province， Dongguan 523326， China； 3.The Joint Center of Guangdong Medical University and Guangdong RunHe Biotechnology Company for Co-enzyme Q10 Research； Guangdong Province， Dongguan 523808， China

[Abstract] Objective To explore the impacts of Co-enzyme Q10 Cream on the blood cell and antioxidant enzymes of mice exposed to ultraviolet ray. Methods 36 KM mice were randomly divided into control group （CON）， model group （MOL）， co-enzyme Q10 group （CoQ）， positive group （POS） group according to weight factor， after them through physical way removed the hair on the backs. The mice in CON were not given to any administration. The mice of MOL， CoQ， POS were respectively smeared blank cream， Co-enzyme Q10 Cream， Benzophenone Cream on the back skin； after 30 minutes， they were exposed to ultraviolet ray， once a day for 8 weeks. All mice were put to death by extracting their eye blood and their blood cells were measured. Results Compared with the CON， while blood cell （WBC） and malondialdehvde （MDA） content of the MOL were increased （P < 0.05）， viability of superoxide dismutase （SOD） and glutathione peroxidase （GSH-Px） was decreased （P < 0.05）. Compared with the MOL， the WBC and MDA content of the CoQ were decreased （P<0.05）， viability of SOD and GSH-Px of the CoQ was increased （P < 0.05）. Compared with the MOL， the WBC and MDA content of the POS were decreased （P<0.05）， viability of SOD and GSH-Px of the POS was increased （P < 0.05）. Conclusion Co-enzyme Q10 Cream can promote the viability of plasma SOD and GSH-Px and reduce the contentof MDA. Co-enzyme Q10 Cream contributes to mitigate the damage towards mice caused by ultraviolet ray.