甘蔗蠟提取物多廿烷醇的降血脂機制研究

廖芳，陳偉偉

摘要：采用RT-PCR、Western Blot、L-[35S]放射性標(biāo)記物摻入等方法，觀察甘蔗蠟提取物多廿烷醇對Hep G2細胞羥甲基戊二酸輔酶A（HMG-CoA）還原酶mRNA轉(zhuǎn)錄及蛋白質(zhì)表達量的影響，及其對HMG-CoA還原酶合成和降解的作用。結(jié)果表明，多廿烷醇降低HMG-CoA還原酶mRNA的轉(zhuǎn)錄和蛋白質(zhì)的表達量，且作用效果隨多廿烷醇濃度的升高而增強。此外，多廿烷醇抑制HMG-CoA還原酶的合成并加速其降解，多廿烷醇處理后HMG-CoA還原酶的半衰期明顯縮短。

關(guān)鍵詞：多廿烷醇；Hep G2細胞；羥甲基戊二酸輔酶A（HMG-CoA）還原酶

中圖分類號：R285.5 文獻標(biāo)識碼：A 文章編號：0439-8114（2013）05-1125-03

Study on the Hypolipidemic Mechanism of Policosanol Extracted from Sugarcane Wax

LIAO Fang，CHEN Wei-wei

（Department of Pharmacology， Gannan Medical University， Ganzhou 341000， Jiangxi， China）

Abstract： The effects of policosanol extracted from sugarcane wax on mRNA transcription and protein expression level， synthesis and degeneration of 3-hydroxy-3-methylglutarl-coenzyme （HMG-CoA） reductase in Hep G2 cell were determined by RT-PCR， Western Blot and L-[35S] radioactive isotope tracing technology. The results showed that the mRNA transcription and protein expression level of HMG-CoA reductase decreased with the increase of policosanol concentration（10，20，30 μg/L）. Policosanol also suppressed the synthesis of HMG-CoA reductases while sped up its degeneration； the half life of HMG-CoA reductases treated by policosanol was shortened obviously.

Key words： policosanol； Hep G2 cell； 3-hydroxy-3-methylglutarl-coenzyme （HMG-CoA） reductase