優(yōu)良益生菌高效篩選與應(yīng)用關(guān)鍵技術(shù)2014年度報(bào)告

田豐偉 孟和 孫志宏 杭曉敏 劉斌

摘 要：益生菌是指一類足量攝入后能對宿主健康水平產(chǎn)生有益影響的活微生物，具有優(yōu)良的生理功能，已成為國際食品生物工程領(lǐng)域的研究熱點(diǎn)。益生菌相關(guān)產(chǎn)品已成為國內(nèi)外食品工業(yè)中一個(gè)飛速增長的領(lǐng)域，具有廣闊的市場前景和社會(huì)價(jià)值。益生菌及其相關(guān)產(chǎn)業(yè)發(fā)展的兩大核心是專利菌種和應(yīng)用關(guān)鍵技術(shù)，在這兩方面我們受制于國外技術(shù)與產(chǎn)品。該研究針對我國益生菌產(chǎn)業(yè)發(fā)展中存在的亟待解決的關(guān)鍵性技術(shù)問題，通過益生菌篩選模型的構(gòu)建與研究以及益生菌產(chǎn)業(yè)化關(guān)鍵技術(shù)的研究，開發(fā)具有特定功能性質(zhì)的新型益生菌菌株，并對其功能進(jìn)行基因定位；在產(chǎn)業(yè)化應(yīng)用方面，著力研究益生菌的發(fā)酵培養(yǎng)、活性保持等關(guān)鍵技術(shù)，為益生菌的產(chǎn)業(yè)化應(yīng)用提供關(guān)鍵技術(shù)支持。

關(guān)鍵詞：益生菌 基因組測序 菌粉 食品生物 益生菌菌株

Abstract：Probiotics refer to a class of live microorganisms that have beneficial impact on the health level of the host， which have good physiological function and become a research hotspot in the field of international food biotechnology. Probiotic products have become a rapidly growing field in the food industry both in china and abroad， which have a broad market prospect and social values. Proprietary species and application of key technologies are two core of probiotics and related industry development， we are subjected to foreign technology and products in both areas. This project for probiotics industry in China to be solved in the development of the key technical problems， through the construction and research of probiotic bacteria screening model and probiotics industrialization key technology research and development with specific functional properties of new probiotic strain， and gene location for its function； In industrial applications， focusing on the research of probiotic fermented culture， active， the key techniques such as the key technology for industrialization of probiotics and application support.

Key Words：Probiotic；Interaction；Genome Sequencing；Bacterial Powder

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