Improvement of copper tolerance of Arabidopsis by transgenicexpressionofanalleneoxidecyclasegene，GhAOC1，in upland cotton（Gossypium hirsutum L.）

Yuange Wang，Huaihua Liu*，Qingguo Xin

aInstitute of Genetics and Developmental Biology，Chinese Academy of Sciences，Beijing 100101，China

bPostdoctoral Research Center of Shandong Shengfeng Seeds Co.，Ltd.，Jiaxiang 272400，China

cYantai Academy of Agricultural Sciences，Yantai 265500，China

Improvement of copper tolerance of Arabidopsis by transgenicexpressionofanalleneoxidecyclasegene，GhAOC1，in upland cotton（Gossypium hirsutum L.）

Yuange Wanga，1，Huaihua Liub，*，1，Qingguo Xinc

aInstitute of Genetics and Developmental Biology，Chinese Academy of Sciences，Beijing 100101，China

bPostdoctoral Research Center of Shandong Shengfeng Seeds Co.，Ltd.，Jiaxiang 272400，China

cYantai Academy of Agricultural Sciences，Yantai 265500，China

A R T I C L E I N F O

Article history:

Received 17 April 2014

Received in revised form

15 February 2015

Accepted 16 February 2015

Available online 11 April 2015

Upland cotton

GhAOC

Expression pattern

Overexpression

Copper tolerance

Allene oxide cyclase（AOC，E 5.3.99.6）is an essential enzyme in the jasmonic acid（JA）biosynthetic pathway and mediates a wide range of adaptive responses.In this report，five AOC genes（GhAOC1-GhAOC5）were cloned from upland cotton（Gossypium hirsutum L.），sequenced，and characterized.Real-time PCR analysis indicated that the transcripts of GhAOCs were abundantly expressed in roots and less in fibers，and regulated in cotton plants under methyl jasmonate（MeJA）and CuCl2stresses.To investigate the role of GhAOC under copper stress，transgenic Arabidopsis plants overexpressing cotton GhAOC1 under control of the Cauliflower mosaic virus 35S（CaMV 35S）promoter were generated.Compared to untransformed plants，GhAOC1-overexpressing Arabidopsis thaliana plants exhibited markedly higher survival rate，shoot fresh weight，shoot dry weight，and photosynthetic efficiency，and reduced cell membrane damage and lipid peroxidation under copper stress. This study provides the first evidence that GhAOC1 plays an important role in copper stress tolerance.

?2015 Crop Science Society of China and Institute of Crop Science，CAAS.Production and hosting by Elsevier B.V.This is an open access article under the CC BY-NC-ND license（http://creativecommons.org/licenses/by-nc-nd/4.0/）.

1.Introduction

Toadaptandsurvive，plantshavedevelopedcomplex defensive mechanisms to cope with a variety of abiotic and biotic stresses，such as pathogen attack，drought，water deficit，salinity，and heavy metals［1］.During plant adaptation to stress，JA，an important signaling chemical，may be generated from lipids.JA and its methyl ester，collectively referred to as jasmonates（JAs）［2］，are important plant endogenous hormones involved in stress response［3，4］.

The oxylipin pathway in plants is responsible for JA synthesis［5］.Under the catalysis of lipoxygenase，molecular oxygen is inserted into α-linolenic acid to produce（13S）-hydroperoxy-（9Z，11E，15Z）-octadecatrienoic acid.This product is dehydrated by a key enzyme，allene oxide synthase（AOS，EC 4.2.1.92）to an unstable allene oxide，which is cyclized by allene oxide cyclase （AOC）to 12-oxo-phytodienoic acid（OPDA）.After by 12-oxo-phytodienoic acid reductase and several cycles of β-oxidation（+）-7-iso-JA is formed.AOC is considered especially important during JA biosynthesis，because its specificity leads to formation of the basic structure of JA through its ability to convert the unstable allene oxide derivative 12，13（S）-epoxyoctadecatrienoic acid（12，13-EOT）to OPDA［6，7］.

AOC genes have been cloned from several plant species，including tomato［7］，mangrove［8］，Arabidopsis thaliana［9］，barley［10］，barrel medic［11］，Hyoscyamus niger［12］，Camptotheca acuminate［13］，Jatropha curcas［14］，Physcomitrella patens［15］，Glycine max［6］，and Leymus mollis［16］.Characterization of these genes from different plants has provided valuable information about theirbiochemical and physiological roles in adaptation to a variety of biotic and abiotic stresses.For example，overexpression of JcAOC in Escherichia coli conferred resistance to salt stress and low temperature［14］.Ectopic overexpression of an AOC gene cloned from C.acuminate using the viral 35S promoter improved tolerance against salt stress and low temperature in transgenic tobacco plants compared to wild-type controls［13］. GmAOC5 transgenic tobaccos showed enhanced tolerance to oxidative stresses，while GmAOC1-expressing transgenic lines showed enhanced salinity stress tolerance［6］.However，the precise mechanisms of AOC function in these plant cell processes，particularly in regulating stress response，remain to be elucidated.

Plant AOC genes show specific and complex expression patterns in multiple organs.In soybean，GmAOC2 showed higher expression only in roots，GmAOC5 was expressed strongly only in stems，and GmAOC3 was expressed highly in flowers［6］.In tomato，the AOC gene is expressed specifically in vascular bundles and the surrounding parenchymatic cells［7］.In contrast，four AOC genes in A.thaliana are constitutively expressed in all leaf tissues［9］.

As one of the most important crops for the textile industry worldwide，cotton often suffers from various environmental stresses，such as drought，soil salinization，and heavy metals.Understanding the molecular basis of stress responses is accordingly a key target of cotton genetic improvement programs.To our knowledge，no reports in the literature have described the effect of GhAOC on abiotic tolerance in upland cotton.In the present study，we cloned five AOC genes，named as GhAOC1-GhAOC5，from cotton for the first time and analyzed their transcription profiles in various tissues and under different stress treatments. GhAOC1 was overexpressed in Arabidopsis and the copper stress tolerance of the transgenic Arabidopsis plants was investigated.

2.Materials and methods

2.1.Plant materials，growth conditions，and treatments

Upland cotton（Gossypium hirsutum cv.Liaomian 9）seeds were surface-sterilized with 75%（v/v）ethanol for 1 min and 10%（v/v）H2O2for 1 h，followed by washing with sterile distilled water.The sterilized seeds were germinated at 23°C for 6 days and the germinated seedlings were transferred to plastic flats containing 13.5 L nutrient solution.The nutrient solution contained（mmol L-1）KH2PO4，0.2；MgSO4，0.5；KCl，1.5；CaCl2，1.5；H3BO3，1×10-3；（NH4）6Mo7O24，5×10-5；CuSO4，5×10-4；ZnSO4，1×10-3；MnSO4，1×10-3；Fe（III）-EDTA，1×10-1.The plants were grown in a controlled-environment growth chamber under the following conditions:16 h light/8 h dark photoperiod with a light intensity of 350 μmol m-2s-1，23°C day and night temperatures，and relative humidity of 60-75%. Two-week-old seedlings with similar heights were transferred to nutrient solution containing 50 μmol L-1MeJA and 120 μmol L-1CuCl2，respectively.At 0，1，3，6，12，24，and 48 h after the stress treatments，the roots were quickly frozen in liquid nitrogen and stored at-70°C for later RNA isolation.

Field grown cotton plants were used to analyze tissuespecific expression of GhAOC.After flowering，leaf，stem，root，petal，anther，stigma，ovule，and fiber tissues were sampled for gene expression analysis.

The wild-type（WT）Arabidopsis plants（Columbia ecotype）used for transformation were grown in a climate chamber at 22°C，70%relative humidity，and with a 16 h light/8 h dark photoperiod with a light intensity of 150 μmol m-2s-1.

2.2.RNA，DNA extraction，and cDNA synthesis

Total RNAs of cotton and Arabidopsis leaves were extracted with TRIzol reagent（Invitrogen，USA）and then treated with DNase I（Promega，USA）.cDNAs were synthesized using oligo-（dT）18as anchor primer and M-MLV reverse transcriptase（Promega，USA）according to the manufacturer's instructions.Genomic DNA was extracted from cotton plants without stress using the cetyltrimethylammonium bromide method［17］.

2.3.Isolation，sequencing，and analysis of GhAOCs

GhAOC genes were obtained by in silico cloning.Four Arabidopsis AOC genes（AtAOC1:NM_113475，AtAOC2:NM_113476，AtAOC3: NM_113477，AtAOC4:NM_101199）and six soybean AOC genes（GmAOC1:HM803106，GmAOC2:HM803107，GmAOC3:HM803108，GmAOC4:HM803109，GmAOC5:HM803110，GmAOC6:HM803111）were used as query probes for BLASTN searches of the public NCBI GenBank database.Based on the resulting sequence of interest，specific primers were designed to amplify the genomic and cDNA sequences of GhAOC in cultivar Liaomian 9（Table 1）. The polymerase chain reaction（PCR）was performed using Pfu DNA polymerase（Promega，Madison，WI，USA）.The isolated genes were sequenced with an ABI3730 DNA Analyzer（Applied Biosystems Inc.，F(xiàn)oster City，CA，USA）.

2.4.Gene structure and bioinformatic analysis

Coding region was identified with ORF Finder（http://www.ncbi. nlm.nih.gov/gorf/gorf.html）.The theoretical isoelectric point（pI）and molecular weight（Mw）of proteins were estimated with the Compute pI/Mw Tool（http://www.expasy.org/tools/pi_tool. html）.The localization of proteins was predicted with PSORT（http://psort.nibb.ac.jp/）.

Table 1-Primer sequences used for GhAOC gene amplification.

2.5.Real-time PCR analysis

Real-time PCR was performed with SYBR Premix Ex Taq II（TaKaRa，Dalian，China）on a Master cycler ep realplex machine（Eppendorf，Hamburg，Germany）.Triplicate quantitative assays were performed for each cDNA sample.GhUbi and AtActin were used as internal reference genes for assessing expression levels in cotton and Arabidopsis，respectively.The relative level of gene expression was calculated according to the method of Livak and Schmittgen［18］.Primer sequences for real-time PCR were listed in Table 2.

2.6.Construction of plant expression vectors and transformation of Arabidopsis

GhAOC was cloned in the pCAMBIA-1302 vector between Bln I and Nco I restriction sites under the control of the CaMV 35S promoter.The vector was transformed into Agrobacterium strain GV3101 by electroporation.The transgenic Arabidopsis plants were generated by the Agrobacterium-mediated floral dip method［19］.Arabidopsis plants were grown in a controlled culture room at 22°C with relative humidity of 60-70%under long-day conditions（16 h light and 8 h dark）.

2.7.Copper tolerance assay

For copper treatment，two T3homozygous plants（OX2 and OX6）and wild-type（WT）seeds were surface-sterilized and grown on MS medium for two weeks，then transferred into plastic pots filled with a 1:1 mixture of perlite and vermiculite and grown for five days before exposure to copper stress.To determine survival rate after copper treatment，three weeks old plants were exposed to copper stress by withholding water and irrigating with 120 μmol L-1CuCl2for 10 days.After treatment，plants were transferred to the normal growth conditions described above.For measurement of survival rate，plants that had green and healthy young leaves were considered to have survived.Maximum photochemical efficiency（Fv/Fm），quantum yield of photosystem II（ΦPSII），and malondialdehyde（MDA）concentration were determined as described previously［20，21］.Plant cell membrane damage rate（MDR）was measured with a microprocessor conductivity meter，DDS-12DW （Lida，Shanghai，China）according to the method of Sairam［22］.MDR（%）=initial electrical conductivity/ electricalconductivity after boiling×100.About45 plants ofeach line were used in each stress experiment，and the experiments were performed at least three times.

2.8.Statistical analysis

ANOVA analysis of the experimental data was performed with SPSS 11.5（SPSS Inc.，Chicago，IL，USA）.

3.Results

3.1.Isolation and sequence analysis of GhAOC genes

Five GhAOC genes were identified and designated as GhAOC1-GhAOC5，respectively.They each contained a single open reading frame，encoding proteins of 253，178，246，245，and 204 amino acids with molecular weights of 27.8，19.8，27.1，27.0，and 22.4 kDa，respectively（Table 3）.The similarities of the GhAOC proteins varied from 32.2%to 67.8%and their identities varied from 22.8%to 56.7%.All five proteins showed localization in the chloroplast by a PSORT analysis（http:// psort.nibb.ac.jp/）.Sequence alignment revealed that the GhAOC proteins shared high similarities in the C terminus，whereas the N-terminal region was divergent（Fig.1）.These divergent N-terminal regions resulted in variations in GhAOC protein length.

Table 2-Primer sequences used for real-time PCR.

Table 3-Overview of the five AOC genes identified in upland cotton.

3.2.Expression of GhAOCs in different tissues and under stress treatments

To investigate their expression profiles in cotton tissues，expressions of the isolated five GhAOC genes in cotton were analyzed by real-time PCR.The relative transcript levels of the GhAOC genes in different tissues were shown in Fig.2.The results showed that the five GhAOC genes were expressed mainly in cotton roots and at moderate to low levels in other tissues.The root-specific expression patterns of these GhAOC genes were probably associated with their functions in roots.

We next investigated the transcriptional responses of GhAOCs under MeJA and CuCl2treatments.Both treatments significantly increased the transcription of several GhAOCgenes compared to the control（0 h）.However，this upregulation was time-dependent，and the expression of different genes peaked at different times after exposure to these stresses.For example，under MeJA treatment，all GhAOC genes were upregulated and peaked at different times，with GhAOC2，GhAOC4，and GhAOC5 reaching the highest expression level in the minimum time（Fig.3）.In the copper stress treatment，GhAOC1 was significantly upregulated by 120 μmol L-1CuCl2，whereas GhAOC5 showed no obvious change（Fig.4）.Collectively，our results indicated a divergence of expression profiles and levels in these five GhAOC genes.

Fig.1-Alignment of GhAOC deduced amino acid sequences.Identical amino acids were shaded in black.

Fig.2-Real-time PCR expression profiles of GhAOC genes in different tissues.Different letters（A to E）indicated statistical significance at the 0.05 probability level among different cotton tissues.Error bars represented standard deviation（SD）of three biological replicates，each was analyzed with three technical replicates.1:roots；2:hypocotyls；3:cotyledons；4:leaves；5:anthers；6:petals；7:ovules；8:fibers.

3.3.GhAOC1 expression conferred copper tolerance in Arabidopsis plants

Because the expression of GhAOC1 was markedly upregulated by CuCl2，we evaluated the copper tolerance of GhAOC1-overexpressing plants under CuCl2stress.Under normal growth conditions，no significant difference was observed in either WT or transgenic seedlings.The positive GhAOC1 transgenic plants were confirmed by RT-PCR（Fig.5-A）.After copper treatment，most WT plants were chlorotic and wilting（Fig.5-B），and the survival rate of the WT plants was only 35.5%，whereas the GhAOC1-overexpressing plants remained green and survived（Fig.5-C）.Compared with the control，copper stress greatly increased JA content，and this increase was much greater in the transgenic than in the WT plants（Fig.5-D）.Under control conditions，there was no significant difference in shoot fresh and dry weights between WT and transgenic plants.After the plants were subjected to copper stress for 10 days，shoot fresh and dry weights were reduced in the WT and transgenic plants，indicating that copper stress impeded plant growth and development.High CuCl2treatment reduced shoot fresh weight and dry weight more severely in WT than in transgenic plants（Fig.5-E，F(xiàn)），implying that overexpressing GhAOC1 increased the tolerance of the plants to copper stress.

3.4.GhAOC1expression led to a reduction in MDA and MDR and improved photosynthetic activity under copper stress in transgenic Arabidopsis plants

To investigate the physiological mechanisms underlying the involvement of GhAOC1 in cotton tolerance to copper stress，F(xiàn)v/Fm，ΦPSII，MDA concentration，and MDR were determined in the youngest expanded leaf before and after copper treatment.The values of Fv/Fm，ΦPSII，MDA，and MDR showed no significant differences between the control and transgenic plants without treatment.Following exposure to copper stress for 10 days，the values of Fv/Fmand ΦPSIIwere significantly reduced.This reduction was much greater in control than in transgenic plants（Fig.6-A，B）.Copper stress greatly increasedMDA concentration and MDR compared to the normal condition，and this increase was much greater in the WT than in the transgenic plants（Fig.6-C，D）.

Fig.3-The expression patterns of GhAOC genes in response to MeJA.Each value is the mean±SD of at least three independent measurements.Different letters indicated a significant difference at the 0.05 probability level.

Fig.4-Expression patterns of GhAOC genes in response to CuCl2.Each value was the mean±SD of at least three independent measurements.Different letters on the same column indicated a significant difference at the 0.05 probability level.

Fig.5-Stress response of GhAOC1-expressing Arabidopsis seedlings.（A）Growth of WT and transgenic lines without or with CuCl2stress.（B）Expression of GhAOC1 in different transgenic Arabidopsis lines.（C）Survival rates of WT and GhAOC1-overexpressing seedlings after copper treatment.（D）JA content of WT and GhAOC1 transgenic plants under copper treatment.（E）Fresh weight of WT and GhAOC1-overexpressing seedlings after copper treatment.（F）Dry weight of WT and GhAOC1-overexpressing seedlings after copper treatment.Different letters indicated a significant difference at the 0.05 probability level.

3.5.Copper-induced expression of GhAOC1in Arabidopsis plants upregulated the JA pathway

The effect of overexpressing GhAOC1 in Arabidopsis on the transcription of genes in the JA pathway was investigated by monitoring the accumulation of OPR3（a JA synthesis enzyme downstream of AOC），Jasmonate-ZIM-Domain Protein1（JAZ1）and AtMYC2（JA signaling genes），and Plant Defensin1.2（PDF1.2， a JA signaling pathway gene）.The expression of these four genes was increased in transgenic compared with WT plants under copper treatment（Fig.7）.

4.Discussion

Previous studies showed that the AOC genes belong to a multigene family.There are four homologous AOC genes in Arabidopsis and six in soybean［6，9］.Tetraploid upland cotton may contain several AOC genes in its large genome，but to date little systematic identification of full-length AOC genes inthis important economic crop has been reported.In the present study，five GhAOC genes were identified in cotton. All five GhAOC proteins are conserved in their C termini and divergent in their N-terminal regions，like other plant AOC proteins［15］.Analysis of tissue-specific expression showed that GhAOCs were abundantly expressed in roots（Fig.3）.The tissue expression profile of GhAOC was in agreement with results in soybean，in which GmAOC2 was expressed predominantly in roots［6］.This finding seems reasonable，given that roots are buried in soil and may sense the presence of copper ions.

Fig.6-Fv/Fm，ΦPSII，MDA concentration，and MDR in plants.（A）Changes in Fv/Fmwith or without copper stress treatment.（B）Values of ΦPSIIbefore and after 120 μmol L-1CuCl2treatment.（C）MDA concentration in transgenic and WT plants after 10 day treatment with 120 μmol L-1CuCl2.（D）MDR of transgenic and WT seedlings treated with 120 μmol L-1CuCl2for 10 days.Different letters indicated a significant difference at the 0.05 probability level.

The dynamic expression patterns of GhAOC were affected by MeJA and CuCl2（Fig.4），suggesting that GhAOCs are involved in the crosstalk of the environmental stress response in cotton.MeJA is an important cellular regulator responding to various environmental conditions in plants［3］.The observation that GhAOCs were upregulated by MeJA（Fig.4-A）suggests that the induction of GhAOC gene by CuCl2-induced stress is associated with the MeJA-mediated signaling pathway.Further work is planned to clarify the molecular mechanism through which the GhAOC proteins interact with the signal transduction system of MeJA.

Plants respond to environmental stress conditions by adjusting various biochemical and physiological processes［23］.The biochemical and physiological basis of tolerance of GhAOC1 transgenic plants was analyzed by studying changes in photosynthetic efficiency，MDR，and MDA concentration under copper stress.After the plants were treated with copper stress for 10 days，survival rates and shoot fresh and dry weights were significantly higher in transgenic lines than in the WT（Fig.5），implying that GhAOC1 conferred tolerance to copper stress.Chlorophyll fluorescence in intact leaves is a reliable，noninvasive method for monitoring photosynthetic changes and reflects the physiological conditions of plants. Fv/Fmis usually used as an important parameter to assess the photooxidative damage to photosystem II（PSII）and decrease in its value indicates damage to PSII［24］.Efficiency of PSII（Φ）［25］measures the amount of absorbed light by chlorophyll molecules associated with PSII system and is described as effective quantum yield（ΦPSII）.Imposition of copper stress resulted in a marked reduction in the Fv/Fmand ΦPSIIvalues of the transgenic plants，and this reduction was greater in the WT plants（Fig.6-A，B）.These observations indicated that overexpression of GhAOC1 resulted in enhanced stability of PSII under copper stress.Maintenance of membrane stability is a major component of environmental stress tolerance［26］. The MDR under stress conditions can be estimated by measurement of electrolyte leakage from plant cells.MDA，produced as a result of peroxidation of membrane lipids，is used as a marker of stress-induced oxidative damage at the cellular level［27］.The results obtained in the present study showed that copper stress for 10 days significantly reduced MDA and MDR in transgenic plants relative to that in WT plants（Fig.6-C，D），clearly showing that lipid peroxidation and cell membrane damage reduction in transgenic plants were caused by overexpression of GhAOC.

Fig.7-Expression levels of OPR3（A），JAZ1（B），AtMYC2（C），and PDF1.2（D）in WT and transgenic plants with or without copper stress.Each value was the mean±SD of at least three independent measurements.Different letters indicated a significant difference at the 0.05 probability level.

To investigate the enhanced copper tolerance at the molecular level，expression levels of several JA signaling genes were measured in transgenic and WT plants with or without copper stress.GhAOC1-overexpressing transgenic Arabidopsis lines displayed increased expression levels of OPR3，JAZ1，AtMYC2，and PDF1.2 under copper stress（Fig.7）. Our results suggested that the higher tolerance to copper stresses observed in GhAOC1-overexpressing Arabidopsis was the result of the increased expression levels of these genes.

In summary，this study identified and characterized five GhAOC genes from upland cotton.GhAOCs were expressed mainly in roots and significantly upregulated by MeJA and CuCl2treatments.Overexpression of GhAOC1 led to increased expression of several JA signaling genes and significantly higher survival rate，JA accumulation，shoot fresh and dry weight，and photosynthetic efficiency under copper stress. GhAOC1 may prove to be a useful gene for molecular breeding of important crops to improve copper stress tolerance.

Acknowledgments

This study was supported by the project of Modern Seed Industry Enterprise Science and Technology Development of Shandong Province，China（SDKJ2012QF003）.

［1］E.E.Farmer，R.R.Johnson，C.A.Ryan，Regulation of expression of proteinase inhibitor genes by methyl jasmonate and jasmonic acid，Plant Physiol.98（1992）995-1002.

［2］C.Wasternack，B.Hause，Jasmonates and octadecanoids: signals in plant stress responses and plant development，Prog.Nucleic Acid Res.72（2002）165-221.

［3］R.A.Creelman，J.E.Mullet，Biosynthesis and action of jasmonates in plants，Annu.Rev.Plant Physiol.Plant Mol. Biol.48（1997）355-381.

［4］S.Parchmann，H.Gundlach，M.J.Mueller，Induction of 12-oxo-phytodienoic acid in wounded plants and elicited plant cell cultures，Plant Physiol.115（1997）1057-1064.

［5］M.Stumpe，I.Feussner，F(xiàn)ormation of oxylipins by CYP74 enzymes，Phytochem.Rev.3（2006）347-357.

［6］Q.Wu，J.Wu，H.Sun，D.Zhang，D.Yu，Sequence and expression divergence of the AOC gene family in soybean: insights into functional diversity for stress responses，Biotechnol.Lett.33（2011）1351-1359.

［7］J.Ziegler，I.Stenzel，B.Hause，H.Maucher，M.Hamberg，R. Grimm，M.Ganal，C.Wasternack，Molecular cloning of allene oxide cyclase:the enzyme establishing the stereochemistry of octadecanoids and jasmonates，J.Biol.Chem.275（2000）19132-19138.

［8］A.Yamada，T.Saitoh，T.Mimura，Y.Ozeki，Expression of mangrove allene oxide cyclase enhances salt tolerance in Escherichia coli，yeast，and tobacco cells，Plant Cell Physiol.43（2002）903-910.

［9］I.Stenzel，B.Hause，O.Miersch，T.Kurz，H.Maucher，H. Weichert，J.Ziegler，I.Feussner，C.Wasternack，Jasmonate biosynthesis and the allene oxide cyclase family of Arabidopsis thaliana，Plant Mol.Biol.51（2003）895-911.

［10］H.Maucher，I.Stenzel，O.Miersch，N.Stein，M.Prasad，U. Zierold，P.Schweizer，C.Dorer，B.Hause，C.Wasternack，The allene oxide cyclase of barley（Hordeum vulgare L.）:cloning and organ-specific expression，Phytochemistry 65（2004）801-811.

［11］S.Isayenkov，C.Mrosk，I.Stenzel，D.Strack，B.Hause，Suppression of allene oxide cyclase in hairy roots of Medicago truncatula reduces jasmonate levels and the degree of mycorrhization with Glomus intraradices，Plant Physiol.139（2005）1401-1410.

［12］K.J.Jiang，Z.H.Liao，Y.Pi，Z.S.Huang，R.Hou，Y.Cao，Q.Wang，X.F.Sun，K.X.Tang，Molecular cloning and expression profile of a jasmonate biosynthetic pathway gene for allene oxide cyclase from Hyoscyamus niger，Mol.Biol.42（2008）381-390.

［13］Y.Pi，K.J.Jiang，Y.Cao，Q.Wang，Z.Huang，L.Li，L.Hu，W.Li，X. Sun，K.Tang，Allene oxide cyclase from Camptotheca acuminata improves tolerance against low temperature and salt stress in tobacco and bacteria，Mol.Biotechnol.41（2009）115-122.

［14］B.Liu，W.Wang，J.Gao，F(xiàn).Chen，S.Wang，Y.Xu，L.Tang，Y.Jia，Molecular cloning and characterization of a jasmonate biosynthetic pathway gene for allene oxide cyclase from Jatropha curcas，Acta Physiol.Plant.32（2010）531-539.

［15］T.Hashimoto，K.Takahashi，M.Sato，P.K.G.S.S.Bandara，K. Nabeta，Cloning and characterization of an allene oxide cyclase，PpAOC3，in Physcomitrella patens，Plant Growth Regul. 65（2011）239-245.

［16］M.E.EltayebHabora，A.E.Eltayeb，M.Oka，H.Tsujimoto，K. Tanaka，Cloning of allene oxide cyclase gene from Leymus mollis and analysis of its expression in wheat-Leymus chromosome addition lines，Breed.Sci.63（2013）68-76.

［17］M.G.Murray，W.F.Thompson，Rapid isolation of high molecular weight plant DNA，Nucleic Acids Res.8（1980）4321-4326.

［18］K.J.Livak，T.D.Schmittgen，Analysis ofrelative gene expression data using real-time quantitative PCR and the 2（-Delta Delta C（T）method，Methods 25（2001）402-408.

［19］S.J.Clough，A.F.Bent，F(xiàn)loral dip:a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana，Plant J.16（1998）735-743.

［20］S.Lal，V.Gulyani，P.Khurana，Overexpression of HVA1 gene from barley generates tolerance to salinity and water stress in transgenic mulberry（Morus indica），Transgenic Res.17（2008）651-663.

［21］R.S.Dhindsa，P.Plumb-Dhindsa，T.A.Thorpe，Leaf senescence correlated with increased levels of membrane permeability and lipid peroxidation，and decreased levels of superoxide dismutase and catalase，J.Exp.Bot.32（1981）93-101.

［22］R.K.Sairam，Effect of moisture stress on physiological activities of two contrasting wheat genotypes，Indian J.Exp. Biol.32（1994）594-597.

［23］B.Singh，H.Chauhan，J.P.Khurana，P.Khurana，P.Singh，Evidence for the role of wheat eukaryotic translation initiation factor 3 subunit g（TaeIF3g）in abiotic stress tolerance，Gene 532（2013）177-185.

［24］K.Maxwell，G.N.Johnson，Chlorophyll fluorescence-a practical guide，J.Exp.Bot.51（2000）659-668.

［25］J.Harbinson，B.Genty，N.R.Baker，Relationship between the quantum efficiencies of photosystems I and II in pea leaves，Plant Physiol.90（1989）1029-1034.

［26］J.Levitt，Responses of plants to environmental stresses: water，radiation，salt and other stresses，Academic Press，New York，1980.

［27］M.Jain，G.Mathur，S.Koul，N.B.Sarin，Ameliorative effects of proline on salt stress induced lipid peroxidation in cell lines of groundnut（Arachis hypogaea L.），Plant Cell Rep.20（2001）463-468.

*Corresponding author.

E-mail address:liuhh@shofine.com（H.Liu）.

Peer review under responsibility of Crop Science Society of China and Institute of Crop Science，CAAS.

1Yuange Wang and Huaihua Liu contributed equally to this work.

http://dx.doi.org/10.1016/j.cj.2015.02.004

2214-5141/?2015 Crop Science Society of China and Institute of Crop Science，CAAS.Production and hosting by Elsevier B.V.This is an open access article under the CC BY-NC-ND license（http://creativecommons.org/licenses/by-nc-nd/4.0/）.