鳥巢蕨組培快繁技術(shù)研究

劉洋

摘要：以鳥巢蕨（Neottopteris nidus）孢子為原材料進(jìn)行組培快繁，考察了孢子處理方式、基本培養(yǎng)基、激素及孢子體誘導(dǎo)方式等條件對(duì)培養(yǎng)效果的影響。結(jié)果表明，把經(jīng)過消毒滅菌的孢子囊從孢子葉上切下，破碎取出孢子后接種到1/2MS培養(yǎng)基上，孢子萌發(fā)較快，萌發(fā)率高。萌發(fā)后的原葉體接種到不添加激素的MS培養(yǎng)基上能得到較大的增殖系數(shù)。原葉體在試管內(nèi)誘導(dǎo)孢子體難度較大，可采用1 g/L的KH2PO4溶液作誘導(dǎo)劑在試管外誘導(dǎo)，有利于原葉體向孢子體的轉(zhuǎn)化。

關(guān)鍵詞：鳥巢蕨（Neottopteris nidus）；孢子；培養(yǎng)基；離體快繁

中圖分類號(hào)：S682.35 文獻(xiàn)標(biāo)識(shí)碼：A 文章編號(hào)：0439-8114（2013）05-1188-02

Study on Rapid Propagation of Neottopteris nidus

LIU Yang

（Department of Plant Science， Jilin Agricultural Science and Technology University， Jilin 132101， Jilin， China）

Abstract： The rapid propagation of Neottopteris nidus by in vitro spore culture was researched. The effects of spore treatment， base medium， plant hormone and sporophyte inducement method on culture efficient were studied. The results showed that if cut off the the sporangia after disinfection from sporophyll， and crushed to release the spores， which were inoculated in 1/2 MS medium， spore germination was with higher rate and faster speed than derect inoculation of sporophyll or sporangia. The propagation coefficient of prothallus was bigger when inoculated in MS medium without any addition of hormone. It was difficult to induce the sporophyte from prothallus by tube culture. However， the transformation of prothallus to sporophyte could be realized by cultivating in substrate in pot with spraying of 1 g/L KH2PO4 inducer.

Key words： Neottopteris nidus； spore； medium； in vitro rapid propagation