Management of type IIIb dens invaginatus using a combination of root canal treatment, intentional replantation, and surgical therapy: A case report

lNTRODUCTlON

Dens invaginatus (DI) is a dental developmental anomaly resulting from enamel enfolding into the dental papilla prior to calcification. DI occurs more often in permanent maxillary lateral incisors and less frequently in mandibular anterior and deciduous teeth[1]. Clinically, pits lined with enamel defects and dentin invaginated into the dental/teeth with DI are susceptible to caries due to microorganism accumulation, easily leading to pulp necrosis and periapical inflammation[2]. However, DI may be easily overlooked due to the absence of clinical signs of malformation.

The most widely known classification of DI into three types was made by Oehler[3] in 1957 based on the extent of invagination from the crown into the root. Type III is the most complicated form due to the more extreme anatomical complexity. Type Ⅲa refers to invagination penetrating through the root and forming a lateral foramen as a pseudoforamen, while type Ⅲb refers to invagination extending through the root with an apical foramen. There is usually no communication with the pulp, which lies compressed within the wall around the invagination process. However, to our knowledge, this is the first report of a tooth of type IIIb DI infecteda lateral canal.

DI poses a great challenge in endodontic treatment due to the development of malformations with distortion of the teeth. Canal irregularities and invagination may prevent mechanical and chemical preparation. Due to the anatomic variations of the complex root canal morphologies, various treatment options have been proposed, including sealing of the invagination, root canal treatment, intentional replantation, periapical microsurgery, and extraction[4-6]. Here, we report a complex case of type Ⅲb DI with a lateral canal. After endodontic treatment, intentional replantation, and periapical microsurgery,the clinical findings and radiographic assessment presented a favorable prognosis of effective periapical healing at a 3-year follow-up assessment.

CASE PRESENTATlON

Chief complaints

A 15-year-old female patient presented to the Department of Endodontics, Anhui Medical University,China with a chief complaint of pain associated with recurrent labial swelling in the area of the left anterior tooth for the past 6 mo. The patient reported that she had undergone endodontic treatment 1 mo earlier with symptom recurrence.

History of present illness

The patient's blood test results were all normal.

History of past illness

3.大力引進(jìn)核心人才和高新技術(shù)，進(jìn)一步拓寬領(lǐng)導(dǎo)干部的知識(shí)面。針對(duì)采油廠開發(fā)復(fù)雜、難度大的實(shí)際，著眼引進(jìn)掌握高新技術(shù)和尖端理論的核心人才，專門從事地質(zhì)勘探課題研究。以前沿知識(shí)和尖端科技進(jìn)一步開闊領(lǐng)導(dǎo)干部的思路，拓寬其知識(shí)面，完善其知識(shí)體系，提高其執(zhí)政能力和領(lǐng)導(dǎo)水平。

Personal and family history

The patient had a noncontributory personal and family history.

Physical examination

Oral examination revealed fluctuating buccal swelling with a sinus at the apical area (Figure 1A). Tooth 22 showed an intact crown with a palatal access cavity (Figure 1B). Sensitivity to vertical percussion and negativity to palpation and electric pulp testing were observed, whereas the response of adjacent teeth was normal. The periodontal probing depths were within the normal range with slight mobility.Radiography showed an invagination of dentin lined by enamel into the pulpal space, expanded space in the middle of the teeth with a large periapical radiolucent lesion, and the presence of bone resorption(Figure 1C).

Laboratory examinations

The patient presented to the Department of Endodontics, Anhui Medical University, China, with a chief complaint of pain associated with recurrent labial swelling in the area of the left anterior tooth for the past 6 mo.

例如，當(dāng)媽媽因?yàn)楹⒆硬宦犜挾鷼鈺r(shí)，先問問自己我現(xiàn)在有什么感受？“如果你察覺到自己在生氣，原因是孩子總故意犯同樣的錯(cuò)誤，你就可以對(duì)自己的情緒做更好的處理。

Imaging examinations

A cone-beam computed tomography (CBCT) scan showed invagination extending from the crown into the root with an apical foramen associated with a large periapical radiolucent lesion, which caused variation in the main root canal. A well-defined unilocular periapical radiolucent lesion was observed.Sagittal CBCT images showed that a loop-shaped chamber separated the main root canal (Figure 1D-G).

FlNAL DlAGNOSlS

A diagnosis of type Ⅲb DI and chronic periodontitis was made.

TREATMENT

Under rubber dam isolation, the temporary filling was removed, and access to the main and invaginated canals was completed. Pulp vitality in the main canal and dens invaginatus canal confirmed necrosis.The total length of the canals was estimated to be 19 mm, and the canals were then thoroughly prepared to apical size 30 by the crown-down technique with a NiTi file. A large volume of pale-yellowish fluid drained from the canal. Following thorough irrigation with 5.25% sodium hypochlorite (NaOCl), the root canals were dried with paper points, and calcium hydroxide paste was placed into the two canals(Figure 2A). The coronal access was sealed with a 3-mm-thick temporary restorative material. The patient was scheduled to return after 2 wk.

The patient reported that she had undergone endodontic treatment 1 mo earlier with symptom recurrence.

Intentional replantation

The authors declare that they have no conflicts of interest to disclose.

Two weeks after the procedure, the patient returned, and the wire and composite resin splint were removed. After removal of the temporary restorative material under rubber dam isolation, calcium hydroxide paste in the root canals was cleared by ultrasonication. After drying the canals with paper points, the canals were fully obturated with gutta percha, and iRoot BP (Innovative BioCeramix Inc.)was performed using a vertical condensation technique. Subsequently, tooth 22 was restored using acidetch and composite resin Z350 (3M ESPE, MN, United States).

Zhang J, Li N, and Li WL analyzed and interpreted the imaging findings; Zhang J and Zheng XY reviewed the literature and drafted the manuscript; Li S was the patient’s dentist and was responsible for the revision of the manuscript; all authors issued final approval for the version to be submitted.

Surgical treatment

Some studies have reported that periradicular inflammation has no relationship with unfilled lateral canals[17], and some authors have argued that an insufficiently obturated lateral canal can lead to endodontic treatment failure. This was attributed to the patency of the lateral canal and microbiological involvement[18]. Kim and Kratchman[19] recommended resecting at least 3 mm of apical root to eliminate 98% of apical ramifications and 93% of lateral canals that exist within 3 mm of the root end.However, in this patient, the foramen of the later canal was located at the middle third of the root.Therefore, removal of 3 mm of root end by surgical treatment would not have benefited lateral periodontitis healing. To our knowledge, this is the first report of a tooth of type IIIb DI infectedthe lateral canal located at the middle of the root.

OUTCOME AND FOLLOW-UP

At the 3-year recall evaluation, the patient had no painful symptoms. The radiograph image presented complete healing of the periapical lesion around the tooth root. Continuity of the periodontal ligament was observed, and intercortical bone had formed completely without any root resorption or ankylosis(Figure 4).

DlSCUSSlON

Treatment for DI varies depending on the invagination type, anatomy of the canal, level of pulp involvement, and morphology of the apex. To our knowledge, this is the first report of an infection of type IIIb DI with a lateral canal resulting in the persistence of periapical damage. Microorganism contamination in the invagination easily inflames the pulp irreversibly and even causes pulp necrosis both in the main canal and the invagination canal, especially in patients with type III DI. In patients with type IIIa DI, endodontic treatment should be performed on the canals as if they were two independent canals[6]. In patients with type IIIb, some authors recommend removing the extremely large anomalous invagination using ultrasonic instruments and combining the two canals as one space to allow for complete and sufficient disinfection[7]. The removal of the single and large canal was followed by obturation with mineral trioxide aggregate in the apical third. However, the elimination of invagination severely compromised the strength of the tooth and increased the risk of tooth fracture.Martins[7] reported that three Fibercore posts were applied to the root canal to reinforce the thin root wall. In this reported case, the invaginated cavity occupied the center of the canal and adhered labially to the canal wall. Removal of the invaginated hard tissue was impractical. Such removal would cause a thin outer shell of dentine to remain, which may result in a poor prognosis.

通用電氣公司把企業(yè)文化視為最好的精神黏合劑，把公司所有人緊緊團(tuán)結(jié)在一起，向著共同的愿景目標(biāo)，協(xié)調(diào)一致，努力奮斗，從而把通用電氣打造成一個(gè)年輕而有活力的企業(yè)。韋爾奇告誡大家：“人才即是一切，有人才的公司才能成為最大的贏家。”“投資于人才”是通用電氣公司核心的管理文化——人才是企業(yè)的核心力量，優(yōu)秀人才是公司最重要的戰(zhàn)略資源，是企業(yè)價(jià)值的主要?jiǎng)?chuàng)造者。在人才全球化戰(zhàn)略的指引下，全球范圍內(nèi)尋找最優(yōu)秀的人才，挖掘有潛力的人才，留住一流的人才，挑選好接班人，始終是通用電氣公司最重要的戰(zhàn)略規(guī)劃。通用電氣公司的每一個(gè)企業(yè)都重用當(dāng)?shù)厝瞬?，同時(shí)注重培養(yǎng)其全球化工作能力，實(shí)現(xiàn)了人才的“全球本土化和本土全球化”。

Successful root canal treatment is based on the complete removal of all infections in the entire root canal system[8]. However, due to the anatomical impediments of type IIIb DI that restrict instrument cleaning and sufficient shaping, chemomechanical preparation cannot effectively eliminate all the infected tissues in the main canal or the invagination canal, including lateral canals and apical ramifications[9]. This would cause root canal treatment to occasionally fail, resulting in the persistence of periapical damage. Since a conventional root canal, as the first alternative, had already been initiated and failed in this patient, surgical endodontic or intentional replantation was the second treatment option instead of tooth extraction[10].

Kong等利用45#鋼平頭彈正撞擊半無限混凝土靶的侵徹實(shí)驗(yàn)[9]，如圖7所示。從圖中可以觀察到彈體在侵徹過程中的3種狀態(tài)。該實(shí)驗(yàn)中，彈擊的沖擊速度為510～1 855 m·s-1，ρp為7 757 kg·m-3，υ為0.3, Y0為380 MPa, Yp為665 MPa。L0為30 mm, r0為3 mm。

Intentional replantation is defined as deliberate extraction of the teeth and visual endodontic manipulation followed by reinsertion back into the original socket. The advantages of intentional replantation are that the major procedure is performed visually and that it benefits inaccessible areas, including the morphological variation in type IIIb DI[11]. A recent systematic review demonstrated that the survival rate of intentional replantation was 90%[12]. In a retrospective study of ten cases of type IIIb DI with apical periodontitis treated by intentional replantation, the survival rate was 80%[13]. Due to its high survival rate, low invasiveness, and cost-effectiveness, intentional replantation is regarded as a reliable and viable treatment when root canal treatment fails. PDL is important for the success of intentional replantation. Yu[14] reported that PDL cells remained alive within 15 to 20 min, which was crucial for the ability of PDL cells to attach to the alveolus. In this patient, we limited the extraoral time to 13 min, leading to successful periradicular healing.

Unfortunately, the sinus tract in the buccal maxillary region was noted at six recall evaluations. In the initial radiograph, we focused on the morphological variation of DI and periapical infection while ignoring the lateral infection caused by organisms in the lateral canal. Considering the patient’s wishes and with the aid of methylene blue staining, surgical treatment was attempted as the last resort to obturate the lateral canal. Lateral canals are accessory canals that usually extend vertically to the main canal. They are not easily visible in preoperative radiographs[9]. Ricucci[9] assessed 500 representative extracted teeth that were scanned by microcomputed tomography. The authors found that lateral canals occurred more frequently in the apical third of the root than in the middle of the root. The shape and tortuosity of the lateral canals may vary among different teeth. Most of the lateral canals were curved and not circular, as reported in this patient, which increased the complexity of the treatment.The median diameter of the lateral canals is 67 μm, while the diameter of the main canals is two to three times larger[15,16]. Therefore, apical periodontitis occurs far more frequently than lateral periodontitis.

The patient refused the extraction and had a strong desire to preserve natural tooth 22. The patient and her parents agreed to perform endodontic microsurgery as a last resort to seal the lateral canal in the mesial of tooth 22. After local anesthesia, a full-thickness triangular mucoperiosteal flap was made to expose the mesial area of tooth 22. The root surface was stained with methylene blue to identify the lateral foramen. An L-shaped pit on the mid-root surface was observed, which was then prepared with an ultrasonic tip and obturated with iRoot BP (Figure 3E and F). The granulomatous tissues were curetted, and the surgical region was irrigated with 0.9% saline. Finally, the flap was repositioned and sutured. The sutures were removed after 2 wk.

要計(jì)算導(dǎo)彈流場(chǎng)和彈頭本身對(duì)光學(xué)系統(tǒng)鏡面上每個(gè)網(wǎng)格節(jié)點(diǎn)的輻射照度, 本文以鏡面上每個(gè)網(wǎng)格節(jié)點(diǎn)為球心建立半球形輻射網(wǎng)格, 往復(fù)循環(huán)計(jì)算出導(dǎo)彈流場(chǎng)對(duì)光學(xué)系統(tǒng)鏡面的輻射照度值. 圖3為流場(chǎng)網(wǎng)格和輻射場(chǎng)網(wǎng)格的相對(duì)位置關(guān)系圖.

盡管是白天，但別墅里的光線卻格外晦暗。羅恬只感覺陳洋的眼睛灼灼地閃著光芒。陳洋輕輕撫著她的臉頰說：“那么多人死了，你不害怕嗎？”

CONCLUSlON

Type IIIb DI has a variety of atypical internal morphologic complexities, which presents a great challenge for treatment in the clinic. This reported case highlights the significance of awareness of type IIIb DI with a lateral canal in the middle of the root. This result indicated that complex type IIIb DI can be successfully treated by a combination of intentional replantation and surgical treatment when conventional endodontic therapy fails.

FOOTNOTES

Unfortunately, 6 mo later, the patient returned pain-free, but the swelling (sinus tract) in the buccal maxillary region reappeared (Figure 3A and B). A gutta percha point in the draining sinus revealed point tracking to a large periapical lesion associated with the mesial surface of tooth 22 (Figure 3C and D).

Health and Medical Research Fund of the Food and Health Bureau, Hong Kong, China, No. 06171376;Natural Science Foundation of Anhui Province, China, No. 2008085MH255; and Scientific Research Funding of Anhui Medical University, China, No.2020xkj148.

Consent was obtained from the patient’s parents for publication of this report.

As endodontic therapy failed and the aberrant anatomic structure hampered adequate disinfection and obturation, the patient was informed that root canal retreatment could not clean out the infected pulp tissue clearly and obturation could not seal the pseudo canal tightly. Intentional replantation and surgical treatment were chosen as the next treatment. After discussing the risk and the financial cost, her parents chose to perform intentional replantation at the 2-wk follow-up appointment (Figure 2B). The patient received 500 mg amoxicillin and 400 mg ibuprofen 30 min before surgery and was instructed to rinse with 0.2% chlorhexidine gluconate solution. After local anesthesia, a minimal portion of tooth 22 was extracted without any fracture. The crown was held by saline-soaked wet gauze to keep the teeth moist and to minimize damage to the periodontal ligament (PDL) (Figure 2C). Under the dental operating microscope, the apex of the tooth was resected with a high-speed handpiece for 3 mm, and the foramen was clearly and deeply prepared by ultrasonic tips. iBoot BP (Innovative BioCeramix Inc.,Vancouver, Canada) was placed to completely seal the retrograde cavity (Figure 2D). Then, the tooth was gently replanted into the socket and fixed by a wire and composite resin splint (Figure 2E). The extraoral times were limited to under 13 min. A radiograph was taken to confirm that the depth of iBoot BP was approximately 5 mm (Figure 2F). After the surgery was performed, amoxicillin (500 mg, 3 times per day) was administered for 3 d to prevent wound infection. The patient was instructed to maintain a soft diet and use a 0.2% chlorhexidine gluconate oral rinse for 2 wk.

增值稅留抵稅額一般是指企業(yè)的進(jìn)項(xiàng)稅抵扣銷項(xiàng)稅之后，由于不能抵扣完全而遺留下來的稅額。留抵稅額在企業(yè)普遍存在，形成的原因主要有三個(gè)，其一是企業(yè)銷售收入并沒有實(shí)現(xiàn)抵扣完全，有采購(gòu)材料的遺留；其二是科技型企業(yè)因?yàn)檠邪l(fā)技術(shù)及其他原因?qū)е落N項(xiàng)稅額不能完全抵扣進(jìn)項(xiàng)稅額；其三是二者稅率的不一致引起了留抵稅額的產(chǎn)生。

墨西哥擁有8屬36種鄉(xiāng)土竹種，其中30種為特有竹種；引進(jìn)竹種30種，主要來自哥倫比亞和加利福尼亞。墨西哥竹子分布于各種各樣的生態(tài)系統(tǒng)中，有些竹種的分布海拔不足900 m，而有些竹種則分布于海拔3 150 m以上的地方[10]。在墨西哥31個(gè)州中有14個(gè)州有竹子的自然分布，其中恰帕斯州、瓦哈卡州、普埃布拉州、塔巴斯科州、哈利斯科州和韋拉克魯斯州是竹子的主要分布地，其他一些州適合竹子生長(zhǎng)，具有引種竹子的潛力。

小學(xué)生好奇心強(qiáng)，在小學(xué)高段作文教學(xué)過程中，教師應(yīng)充分利用小學(xué)生的這一特點(diǎn)，引導(dǎo)學(xué)生將自己感興趣的內(nèi)容記錄下來，通過對(duì)事物最初的認(rèn)知及變化，有效鍛煉學(xué)生的思維能力和理解能力。在這一過程中，教師應(yīng)為學(xué)生提供豐富的感性材料。結(jié)合體驗(yàn)式教學(xué)方法的特點(diǎn)，教師可以通過喚起學(xué)生記憶，引導(dǎo)學(xué)生搜集大量感性材料。

The authors have read the CARE Checklist (2016), and the manuscript was prepared and revised according to the CARE Checklist (2016).

This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BYNC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is noncommercial. See: https://creativecommons.org/Licenses/by-nc/4.0/

China

Jing Zhang 0000-0002-2774-8110; Na Li 0000-0002-0091-7797; Wu-Li Li 0000-0002-3684-7057; Xian-Yu Zheng 0000-0002-1631-8641; Song Li 0000-0003-1736-2852.

Yan JP

Wang TQ

例如，關(guān)于“細(xì)胞為什么不能無限長(zhǎng)大”的討論，教師可以布置以下學(xué)習(xí)任務(wù)：假設(shè)細(xì)胞為正方體，以邊長(zhǎng)分別為 1、2、3、4 μm,計(jì)算細(xì)胞的表面積和體積比（表1），嘗試發(fā)現(xiàn)其中的規(guī)律。

Yan JP