Sirtuin 1 alleviates endoplasmic reticulum stress-mediated apoptosis of intestinal epithelial cells in ulcerative colitis

Meng-Ting Ren, Meng-Li Gu, Xin-Xin Zhou, Mo-Sang Yu, Hang-Hai Pan, Feng Ji, Chen-Yan Ding

Abstract BACKGROUND Sirtuin 1 (SIRT1) is a nicotinamide adenine dinucleotide (NAD+)-dependent protein deacetylase that is involved in various diseases, including cancers,metabolic diseases, and inflammation-associated diseases. However, the role of SIRT1 in ulcerative colitis (UC) is still confusing.AIM To investigate the role of SIRT1 in intestinal epithelial cells (IECs) in UC and further explore the underlying mechanisms.METHODS We developed a coculture model using macrophages and Caco-2 cells. After treatment with the SIRT1 activator SRT1720 or inhibitor nicotinamide (NAM), the expression of occludin and zona occludens 1 (ZO-1) was assessed by Western blot analysis. Annexin V-APC/7-AAD assays were performed to evaluate Caco-2 apoptosis. Dextran sodium sulfate (DSS)-induced colitis mice were exposed to SRT1720 or NAM for 7 d. Transferase-mediated dUTP nick-end labeling(TUNEL) assays were conducted to assess apoptosis in colon tissues. The expression levels of glucose-regulated protein 78 (GRP78), CCAAT/enhancerbinding protein homologous protein (CHOP), caspase-12, caspase-9, and caspase-3 in Caco-2 cells and the colon tissues of treated mice were examined by quantitative real-time PCR and Western blot.RESULTS SRT1720 treatment increased the protein levels of occludin and ZO-1 and inhibited Caco-2 apoptosis, whereas NAM administration caused the opposite effects. DSS-induced colitis mice treated with SRT1720 had a lower disease activity index (P ＜ 0.01), histological score (P ＜ 0.001), inflammatory cytokine levels (P ＜ 0.01), and apoptotic cell rate (P ＜ 0.01), while exposure to NAM caused the opposite effects. Moreover, SIRT1 activation reduced the expression levels of GRP78, CHOP, cleaved caspase-12, cleaved caspase-9, and cleaved caspase-3 in Caco-2 cells and the colon tissues of treated mice.CONCLUSION SIRT1 activation reduces apoptosis of IECs via the suppression of endoplasmic reticulum stress-mediated apoptosis-associated molecules CHOP and caspase-12.SIRT1 activation may be a potential therapeutic strategy for UC.

Key words: Sirtuin 1; Endoplasmic reticulum stress; Apoptosis; Ulcerative colitis;Intestinal barrier

INTRODUCTION

Ulcerative colitis (UC), the main subtype of inflammatory bowel disease (IBD), is a chronic relapsing inflammatory disorder of the large intestine. The incidence and prevalence of UC have increased in recent years[1]. The etiology of UC remains obscure and involves a combination of genetics, environment, microbiota, and the immune system[2,3]. It is widely believed that dysregulation of cytokines (e.g., tumor necrosis factor-α (TNF-α), interleukin-1 (IL-1), IL-10, and IL-21), oxidative stress, and abnormal immune responses are key players in the progression of UC[4]. Recent data demonstrate that the intestinal epithelium, which plays a crucial role in the occurrence and persistence of UC, is a highly dynamic tissue rather than a simple physical barrier[5]. Although a large number of therapeutic agents, including 5-ASA drugs, immunosuppressants, steroids, and emerging biological agents, have appeared in the past few years, most patients still experience severe complications or recurrence of the disease, which greatly reduces their quality of life[6]. Therefore, it is imperative to develop effective treatments for UC.

The endoplasmic reticulum (ER) is a principal compartment in eukaryotic cells for protein folding and trafficking. Cellular stresses such as perturbations of Ca2+homeostasis and oxidative stress disrupt ER homeostasis, resulting in the accumulation of unfolded and misfolded proteins in the ER lumen, which initiates the unfolded protein response (UPR)[7]. The UPR reduces protein synthesis, accelerates protein folding, and activates ER-associated degradation to orchestrate the recovery of ER function. However, if ER stress is too severe or persistent, intrinsic apoptotic pathways are eventually triggered, leading to cell death[8,9]. Recently, increasing evidence suggests that ER stress and UPR are involved in the pathogenesis of UC by regulating apoptosis, autophagy, and inflammatory responses[6,10].

In the present study, we aimed to investigate the role of SIRT1 in the intestinal barrier in a UC coculture model and in mice with dextran sodium sulfate (DSS)-induced colitis. The mechanisms underlying the effect of SIRT1 on ER stress-mediated apoptotic pathways within intestinal epithelial cells (IECs) were further explored.

MATERIALS AND METHODS

Reagents

The SIRT1 activator SRT1720 and inhibitor nicotinamide (NAM) were obtained from Selleck Chemicals (Houston, TX, United States). DSS was purchased from MP Biomedical (Santa Ana, CA, United States). Anti-occludin, anti-zona occludens 1 (ZO-1), and anti-caspase-3 primary antibodies were purchased from Proteintech (Wuhan,China), anti-caspase-12 and anti-caspase-9 antibodies were obtained from LSBio(Seattle, WA, United States), and anti-SIRT1, anti-glucose-regulated protein 78(GRP78), anti-CCAAT/enhancer-binding protein homologous protein (CHOP), and anti-β-actin antibodies were obtained from Abcam (Cambridge, UK).

Cell culture and coculture

We established an in vitro coculture model of Caco-2 and THP-1 cells based on previous studies[17-19].The human colon carcinoma Caco-2 and monocyte THP-1 cell lines were obtained from the American Type Culture Collection (ATCC; Manassas,VA, United States), cultured in Dulbecco's modified Eagle's medium (DMEM; Gibco,Carlsbad, CA, United States) and RPMI-1640 cell culture medium (Gibco),respectively, supplemented with 10% fetal bovine serum (FBS; Gibco), and incubated at 37 °C in a 5% CO2atmosphere. To establish the coculture model, Caco-2 cells were cultured in 6-well culture inserts (Transwell inserts; Corning Costar, NY, United States) at a density of 2 × 105cells/insert for 17-20 d to obtain an integrated monolayer. THP-1 cells were cultured in 6-well plates at a density of 1.5 × 106cells/well and treated with serum-free RPMI-1640 medium containing 100 ng/mL phorbol-12-myristate-13-acetate (PMA; Sigma-Aldrich, St. Louis, MO, United States)and 0.3% bovine serum albumin (BSA; Sigma-Aldrich) for 48 h. After confirming that THP-1 cells had differentiated into macrophages, the Transwell insert on which Caco-2 cells had been cultured for 17-20 d was placed in the culture well in which human macrophage-like THP-1 cells were cultivated, then lipopolysaccharide (LPS; Sigma-Aldrich) was added to the lower chamber at a final concentration of 10 ng/ml.Ultimately, the two cell lines were cocultured for 24 h. Once the coculture model was established, the SIRT1 activator SRT1720 or inhibitor NAM was added to the upper chamber medium at a final concentration of 10 μM and 5 mM, respectively.

Enzyme-linked immunosorbent assay (ELISA)

The levels of secreted inflammatory cytokines IL-1β and TNF-α in the coculture model as well as in the colon tissues of treated mice were assayed using ELISA kits(Boster, Wuhan, China) according to the manufacturer's instructions. Cell-free supernatants from the upper chamber after coculture for 24 h and colon homogenate supernatants of mice were collected. The absorbance at 450 nm was detected with a microplate reader (Thermo Fisher Scientific, Waltham, MA, United States).

Annexin V-APC/7-AAD assays

Caco-2 apoptosis was evaluated with an Annexin V-APC/7-AAD Apoptosis Detection kit (Keygen Biotech, Nanjing, China). After SRT1720 or NAM treatment for 48 h, Caco-2 cells were harvested with EDTA-free trypsin, washed twice with cold phosphate-buffered saline, and resuspended in 500 μL of 1 × binding buffer. The resuspended cells were incubated with 5 μL of Annexin V-APC and 5 μL of 7-AAD for 5 min in the dark prior to being analyzed with a CytoFLEX flow cytometer(Beckman Coulter, CA, United States).

Animals

Twenty-four female C57BL/6 mice (6-8 wk old, weighing 18-22 g) were obtained from SIPPR-BK Lab Animal Co. Ltd. (Shanghai, China) and kept at room temperature (22-23 °C), with a light/dark cycle of 12/12 h, and free access to food and water. All experimental protocols were designed to minimize pain or discomfort to the animals and were approved by the Ethics Committee of the First Affiliated Hospital, College of Medicine, Zhejiang University.

Female mice were randomly divided into four groups of six per group: The control group had free access to drinking water; the UC group was fed 3% DSS (w/v) for 7 consecutive days; and the UC + SRT1720 and UC + NAM groups received 3% DSS(w/v) for 7 consecutive days, followed by treatment with SRT1720 (100 mg/kg·d,intraperitoneal injection) or NAM (500 mg/kg·d, intraperitoneal injection) for another 7 d, respectively. The disease activity index (DAI) was measured daily after successful induction of acute colitis, as previously described[20].

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All mice were sacrificed by decapitation. Distal colon samples were harvested for subsequent studies. The histological score (HS) of colon sections stained with hematoxylin and eosin was evaluated as described previously[20].

Transferase-mediated dUTP nick-end labelling (TUNEL) assay

Apoptosis of cells in the colon tissue was assessed using a commercially available TUNEL assay kit (In Situ Cell Death Detection kit; Roche Applied Science, Basel,Switzerland) according to the manufacturer's instructions. In brief, tissue sections were incubated with proteinase K solution at 37 °C for 15 min. Afterwards, the enzyme solution and label solution were mixed (1:9) and added to the samples. The addition of 50 μL of converter-POD for 30 min was performed sequentially. Ten fields per section were assayed randomly in each experiment, and the percentage of positive cells was calculated.

Quantitative real-time PCR (qRT-PCR)

Total RNA was extracted from human macrophage-like THP-1 and Caco-2 cells using TRIzol reagent (TaKaRa, Shiga, Japan) and reverse transcribed using a PrimeScriptTMRT reagent kit with gDNA Eraser (TaKaRa). qRT-PCR was performed on an Applied Biosystems 7500 Fast Real-Time PCR System using the SYBER Green Premix Ex Taq kit (TaKaRa) according to the manufacturer's protocol. The primer sequences are listed in Table 1. The relative mRNA expression was analyzed by the 2-Ctmethod.

Western blot analysis

Total protein from Caco-2 cells and colon segments of mice was isolated with RIPA buffer (Beyotime Biotechnology, Shanghai, China). Protein was quantified by BCA assay, separated by 10% SDS-PAGE, and transferred to polyvinylidene difluoride membranes (Millipore, Billerica, MA, United States). The membranes were blocked with 5% BSA diluted in TBS containing 5% Tween-20 for 2 h at room temperature,and incubated with primary antibodies at 4 °C overnight. Finally, the membranes were treated with ECL reagent and exposed to X-ray film. β-actin was used as an internal control.

Statistical analysis

Data are shown as the mean ± standard deviation (SD). All statistical analyses were performed with GraphPad Prism 7.0 (GraphPad Software, San Diego, United States)using unpaired Student's t-test or one-way analysis of variance followed by Tukey's test for multiple comparisons. P ＜ 0.05 indicated a statistically significant difference.

RESULTS

Establishment of a coculture model in vitro

Cell-free supernatants from the upper chamber, human macrophage-like THP-1 cells,and Caco-2 cells were collected for the evaluation of IL-1β and TNF-α levels by ELISA or qRT-PCR. In the present study, the levels of secreted IL-1β and TNF-α in the upper chamber supernatants were dramatically increased upon LPS stimulation (Figure 1A and B; P ＜ 0.001 vs coculture). In addition, the mRNA expression levels of inflammatory cytokines in macrophages and Caco-2 cells were significantly increased compared with the cells cocultured without added LPS (Figure 1C; P ＜ 0.01 for IL-1β in Caco-2 vs coculture, P ＜ 0.001 for IL-1β and TNF-α in macrophages and TNF-α in Caco-2 vs coculture). Herein, LPS administration for 24 h significantly increased IL-1β and TNF-α levels in the coculture model, which is in accordance with precious studies[17-19], suggesting that it is a suitable model to mimic acute colitis in vitro.

SIRT1 activation enhances tight junctions in Caco-2 monolayers

To assess the integrity of the intestinal barrier, we detected the expression levels oftight junction (TJ) proteins occludin and ZO-1. After coculturing with SRT1720 (10 μM) or NAM (5 mM), Caco-2 cells were collected, and the protein was extracted for Western blot analysis. As expected, compared with the UC group, SRT1720 administration significantly upregulated the expression of the TJ proteins occludin and ZO-1, while NAM treatment suppressed the expression of occludin and ZO-1(Figure 2). Clearly, our data indicate that drug treatment for 48 h results in the strongest protective and damaging effect on Caco-2 monolayers, respectively.Therefore, we chose 48 h as the time point for drug treatment in subsequent experiments.

Table 1 Primer sequences for quantitative real-time polymerase chain reaction

SIRT1 inhibits Caco-2 apoptosis

Annexin V-APC/7-AAD staining assays were applied to assess the apoptosis of Caco-2 cells treated with SRT1720 or NAM for 48 h. As shown in Figure 3A, the induction of colitis caused significant damage to the Caco-2 monolayers, with the apoptosis rate(Annexin V-APC+/7-AAD+ quadrant and Annexin V-APC+/7-AAD- quadrant)reaching 10.21%. Though administration of SRT1720 reduced the apoptosis rate of Caco-2 cells to some extent, there were no significant differences between the UC +SRT1720 group and the UC group (Figure 3B). However, NAM treatment led to a significant increase in the rate of apoptosis (Figure 3B; P ＜ 0.001 vs UC).

SIRT1 negatively regulates ER stress-mediated apoptotic pathways in Caco-2 monolayers

As shown in Figure 4A, SRT1720 administration increased the protein level of SIRT1,while NAM treatment downregulated its expression. To explore the molecular mechanisms underlying the protective role of SIRT1, we detected the mRNA levels of the ER stress chaperone GRP78 and the ER stress-induced apoptosis marker CHOP in Caco-2 cells. Exposure to SRT1720 for 48 h resulted in significantly decreased mRNA expression levels of GRP78 (Figure 4B; P ＜ 0.001 vs UC) and CHOP (Figure 4C; P ＜0.01 vs UC), whereas NAM treatment increased the expression of GRP78 and CHOP(Figure 4B and C; P ＜ 0.01 vs UC). Western blot was also performed to verify the protein levels of ER stress- and apoptosis-related molecules. Consistent with the mRNA levels, we found that the level of GRP78 was significantly decreased in the SRT1720-treated group compared with the UC group (Figure 4D). In addition, the levels of CHOP and cleaved caspase-12, which play important roles in ER stressinduced apoptosis, were also decreased after SRT1720 treatment (Figure 4D).Moreover, the expression of downstream molecules, such as caspase-9 and caspase-3,was also suppressed (Figure 4D). In contrast, NAM treatment increased the expression of GRP78 and CHOP and upregulated the levels of the activated forms of caspase-12, caspase-9, and caspase-3 (Figure 4D).

SIRT1 clinically and histologically ameliorates DSS-induced colitis

Figure 1 Establishment of a coculture model in vitro. A: The levels of secreted IL-1β in the upper chamber supernatants were detected by ELISA; B: The levels of secreted TNF-α in the upper chamber supernatants were detected by ELISA; C: The mRNA expression levels of IL-1β and TNF-α in macrophage-like THP-1 cells and Caco-2 cells were tested by quantitative real-time PCR. Data are presented as the mean ± SD. bP ＜ 0.01 vs coculture; cP ＜ 0.001 vs coculture. LPS:Lipopolysaccharide; IL-1β: Interleukin-1β; TNF-α: Tumor necrosis factor-α; ELISA: Enzyme-linked immunosorbent assay; PCR: Polymerase chain reaction.

Symptoms of acute colitis, including weight loss, diarrhea, and rectal bleeding, were observed daily after 7 d of DSS exposure. As expected, the administration of DSS successfully induced colitis, as the DAI dramatically increased in the UC group compared with the control group (Figure 5A; P ＜ 0.001 vs control). The DAI score was higher in the UC + NAM group than in the UC group (Figure 5A; P ＜ 0.01 vs UC), and SRT1720 treatment markedly reduced the DAI score (Figure 5A; P ＜ 0.01 vs UC, P ＜0.001 vs UC). Histologically, integrity loss, goblet cell damage, and inflammatory cell infiltration were observed in the DSS group compared with the control group (Figure 5B and C; P ＜ 0.001 vs control). Compared with those in the UC group, the above changes were ameliorated in the SRT1720-treated group and aggravated in the NAMtreated group (Figure 5B). The HS of the UC + SRT1720 group was higher than that of the UC group (Figure 5C; P ＜ 0.001 vs UC), while the UC + NAM group did not show significantly aggravated colitis (Figure 5C). Taken together, these data show that SIRT1 activation reduces susceptibility to DSS-induced acute colitis both clinically and histologically.

SIRT1 decreases inflammatory cytokine expression in DSS-induced colitis

The expression levels of IL-1β and TNF-α in colon tissues were detected by ELISA to assess the inflammatory response. The data indicated that DSS treatment increased the levels of IL-1β and TNF-α significantly (Figure 6A and B; P ＜ 0.001 vs control).Reduced expression levels of inflammatory cytokines were observed in the UC +SRT1720 group compared with the UC group (Figure 6A and B; P ＜ 0.01 for IL-1β and P ＜ 0.001 for TNF-α vs UC). In addition, the UC + NAM group showed increased expression levels of IL-1β and TNF-α (Figure 6A and B; P ＜ 0.05 vs UC).

SIRT1 activation reduces the apoptotic cell rate in DSS-induced colitis

To estimate apoptosis in the colon tissue, TUNEL assays were performed, and positively stained cells were counted. The DSS group presented a dramatically larger number of apoptotic cells than the control group (Figure 7A and B; P ＜ 0.001 vs control). There were fewer apoptotic cells in the UC + SRT1720 group than in the UC group (Figure 7A and B; P ＜ 0.01 vs UC). Moreover, NAM administration decreased the percentage of apoptotic cells (Figure 7A and B; P ＜ 0.001 vs UC).

SIRT1 activation suppresses ER stress-mediated apoptotic pathways during DSSinduced colitis

Protein levels in colon tissues of treated mice were assessed by Western blot. DSS administration caused a lower protein level of SIRT1. Besides, SRT1720 and NAM treatment upregulated and downregulated SIRT1 expression levels, respectively(Figure 8A). As shown in Figure 8B, compared with the control group, the DSStreated group showed significantly elevated protein levels of GRP78, CHOP, and cleaved caspase-12, suggesting that ER stress and the UPR were activated. Consistent with the in vitro results, SRT1720 administration downregulated the protein levels of GRP78, CHOP, cleaved caspase-12, cleaved caspase-9, and cleaved caspase-3 (Figure 8B). Additionally, NAM administration caused the opposite effect (Figure 8B).Altogether, these results show that SIRT1 activation inhibits ER stress-mediated apoptotic pathways in DSS-induced colitis mice.

Figure 2 Sirtuin 1 activation enhances tight junctions in Caco-2 monolayers. The protein levels of occludin and ZO-1 in Caco-2 monolayers were examined after administration of the drug for 24 h, 48 h, or 72 h. UC: Ulcerative colitis; NAM: Nicotinamide; ZO-1: Zona occludens 1.

DISCUSSION

UC is characterized by chronic colonic mucosal inflammation and is a known risk for colorectal cancer. Although novel pharmacological therapies have been emerging recently, the existing treatments for UC are still not satisfactory[21]. Thus, developing effective drugs is essential. As a well-known modulator of lifespan, SIRT1 is involved in various diseases, including cancers, metabolic diseases, and inflammationassociated diseases[22-25]. However, the role of SIRT1 in the intestinal barrier and intestinal inflammation is still obscure. Here, we applied the SIRT1 activator SRT1720 and inhibitor NAM to investigate the potential effects of SIRT1 on the intestinal barrier in a UC coculture model and in mice with DSS-induced colitis. Our results demonstrate that the pharmacological activation of SIRT1 enhances TJ integrity of the intestinal barrier and reduces apoptosis of IECs by downregulating the expression of CHOP and suppressing the activation of caspase-12, which are key molecules in ER stress-mediated apoptotic pathways.

SIRT1 was found to be downregulated in colonic epithelium and lamina propria mononuclear cells (LPMCs) of IBD patients and elevated after successful infliximab treatment, suggesting that SIRT1 is involved in the development of IBD[13,14].Furthermore, previous studies have identified the protective role of SIRT1 in intestinal inflammation, the molecular mechanisms of which include intestinal microbiota alteration and nuclear factor kappa B (NF-κB) pathway suppression[13,16,26]. In our study, SIRT1 activation significantly alleviated DSS-induced experimental colitis both clinically and histologically; this alleviation of colitis was accompanied by the downregulation of the levels of the inflammatory cytokines IL-1β and TNF-α in colon tissues, which is consistent with previous studies. Nevertheless, further studies are required to illuminate the underlying mechanisms.

The intestinal epithelial barrier isolates the internal milieu from the external environment and plays a crucial role in intestinal homeostasis. Intestinal inflammation is associated with increased permeability of the intestinal mucosa caused by intestinal barrier damage[27]. Occludin and ZO-1 are important TJ proteins and are essential for the maintenance of intestinal mucosal barrier integrity[28].Occludin interacts directly with claudins and actin and takes part in the regulation of the intestinal barrier[29]. ZO-1 is a peripheral membrane protein that is essential for TJ assembly and maintenance[29]. A recent study revealed that occludin and ZO-1 expression in UC patients was not only significantly decreased compared with that of healthy controls but also positively related to intestinal mucosal healing[30]. Moreover,previous studies have demonstrated that SIRT1 enhances TJs in other physical barriers[31,32]. Herein, we found that SIRT1 activation significantly increased the expression of occludin and ZO-1 in Caco-2 monolayers, suggesting that SIRT1 may exert protective effects on colitis by promoting intestinal barrier integrity.

Figure 3 Sirtuin 1 inhibits Caco-2 apoptosis. A: Cells in the upper-right (UR) portion are late apoptotic cells, whereas cells in the lower-left and lower-right (LR)portions are viable and early apoptotic cells, respectively; B: Apoptotic rate (UR + LR) of Caco-2 cells. Each value is presented as the mean ± SD. cP ＜ 0.001 vs UC.UC: Ulcerative colitis; NAM: Nicotinamide.

IECs, which comprise enterocytes, goblet cells, and Paneth cells, have welldeveloped ER structures for the biosynthesis of large amounts of proteins. Increasing evidence suggests that ER stress and the UPR in IECs are involved in the pathogenesis of UC[10,33,34]. Unresolved ER stress is a common character of the UC epithelium and results in the activation of the UPR to restore ER homeostasis or the induction of cell apoptosis if ER stress is too severe to be rescued[35]. Additionally, ER stress in IECs is related to intestinal dysbiosis and dysregulated immune response, leading to cell dysfunction, mucosal barrier damage, and intestinal inflammation[36]. In the present study, exposure to DSS increased the expression of the ER stress marker GRP78,which was accompanied by an increased rate of apoptosis in the colon and the activation of apoptosis-related proteases caspase-9 and caspase-3. GRP78 is an ER chaperone that binds to transmembrane ER stress sensors (inositol-requiring enzyme 1α (IRE1α), double-stranded RNA-activated protein kinase-like ER kinase (PERK),and activating transcription factor 6α (ATF6α)) in non-stressed cells[37]. It is released and contributes to the apoptosis of IECs upon ER stress[6]. Moreover, we found that SIRT1 activation reduced apoptosis in Caco-2 monolayers and colon tissues of DSSinduced colitis mice, indicating that SIRT1 may play a protective role in ER stressinduced injury.

To further confirm the protective effect of SIRT1, we detected the protein levels of CHOP and caspase-12. As a downstream transcriptional factor of PERK/eukaryotic translation initiation factor 2α (eIF2α)/activating transcription factor 4 (ATF4), CHOP plays a critical role in ER stress-induced apoptosis through suppression of the antiapoptotic protein Bcl-2 and induction of the proapoptotic molecules Bim, death receptor 5 (DR5), and telomere repeat binding factor 3 (TRB3)[38]. The intestinal epithelium of IBD patients shows higher expression of CHOP compared with normal people[39]. Furthermore, CHOP overexpression increases susceptibility to intestinal inflammation and mucosal tissue injury in mice, whereas knockdown of CHOP alleviates IEC apoptosis[40,41]. Previous studies have revealed that SIRT1 alleviates ER stress-mediated cell apoptosis through the downregulation of the PERK-eIF2α-CHOP axis in the UPR pathway in cardiac cells and chondrocytes[42,43]. Caspase-12 is another marker of ER stress-mediated apoptosis, which is separated from the ER membrane and cleaved into active fragments upon ER stress, resulting in caspase-3 cleavage and apoptosis[44]. Guo et al[45]reported that SIRT1 may alleviate ER stress-mediated apoptosis of cardiomyocytes via reduced expression levels of CHOP and cleaved caspase-12. Here, we show that SIRT1 activation decreases the expression of CHOP and suppresses the activation of caspase-12 in Caco-2 cells as well as in DSS-induced colitis mice, while treatment with the SIRT1 inhibitor NAM induced the opposite effect, which indicates that SIRT1 protects IECs from ER stress-induced apoptosis by suppressing CHOP, caspase-12, and their downstream signaling cascades.

Figure 4 Sirtuin 1 negatively regulates endoplasmic reticulum stress-mediated apoptotic pathways in Caco-2 monolayers. A: The protein levels of SIRT1 in Caco-2 cells were detected by Western blot; B: The mRNA levels of GRP78 in Caco-2 cells were tested by quantitative real-time PCR; C: The mRNA levels of CHOP in Caco-2 cells were tested by quantitative real-time PCR; D: The protein levels of GRP78, CHOP, caspase 12, caspase 9, and caspase 3 were detected by Western blot in Caco-2 cells. Data are presented as the mean ± SD. bP ＜ 0.01 vs UC; cP ＜ 0.001 vs UC. UC: Ulcerative colitis; NAM: Nicotinamide; PCR: Polymerase chain reaction; GRP78: Glucose-regulated protein 78; CHOP: CCAAT/enhancer-binding protein homologous protein; SIRT1: Sirtuin 1.

In conclusion, we discovered that SIRT1 activation contributes to enhanced intestinal barrier integrity and reduced IEC apoptosis via the suppression of ER stressmediated apoptotic proteins such as CHOP and caspase-12. SIRT1 may serve as a novel drug target, and SIRT1 activation is a promising therapeutic strategy for UC.

Figure 5 Sirtuin 1 clinically and histologically ameliorates dextran sodium sulfate-induced colitis. A: Disease activity index was calculated; B: Hematoxylineosin staining of colon sections was performed (×100); C: Histological scores of stained sections were calculated. Each value is presented as the mean ± SD. bP ＜0.01 vs UC; cP ＜ 0.001 vs UC; dP ＜ 0.001 vs control. UC: Ulcerative colitis; NAM: Nicotinamide.

Figure 6 SlRT1 decreases inflammatory cytokine expression in dextran sodium sulfate-induced colitis. The expression levels of IL-1β and TNF-α were detected by ELISA. A: The expression of IL-1β in colon tissues; B: The expression of TNF-α in colon tissues. Data are presented as the mean ± SD. aP ＜ 0.05 vs UC;bP ＜ 0.01 vs UC; cP ＜ 0.001 vs UC; dP ＜ 0.001 vs control. UC: Ulcerative colitis; NAM: Nicotinamide; IL-1β: interleukin-1β; TNF-α: Tumor necrosis factor-α; ELISA:Enzyme-linked immunosorbent assay.

Figure 7 SlRT1 activation reduces apoptotic cell rates in dextran sodium sulfate-induced colitis. A: TUNEL staining of colon tissue was performed; B: The number of TUNEL positive cells per 10000 cells was counted. Each value is presented as the mean ± SD. bP ＜ 0.01 vs UC; cP ＜ 0.001 vs UC; dP ＜ 0.001 vs control.TUNEL: Transferase-mediated dUTP nick-end labeling; UC: Ulcerative colitis; NAM: Nicotinamide.

Figure 8 Sirtuin 1 activation suppresses endoplasmic reticulum stress-mediated apoptotic pathways during dextran sodium sulfate-induced colitis. The protein levels of SIRT1, GRP78, CHOP, cleaved caspase-12, cleaved caspase-9, and cleaved caspase-3 in colon tissues were detected by Western blot. UC:Ulcerative colitis; NAM: Nicotinamide; GRP78: Glucose-regulated protein 78; CHOP: CCAAT/enhancer-binding protein homologous protein; SIRT1: Sirtuin 1.

ARTICLE HIGHLIGHTS

Research background

Ulcerative colitis (UC), the main subtype of inflammatory bowel disease (IBD), is a chronic relapsing inflammatory disorder of the large intestine. The incidence and prevalence of UC have increased in recent years. Sirtuin 1 (SIRT1), a member of the mammalian sirtuin family of proteins, is a nicotinamide adenine dinucleotide (NAD+)-dependent protein deacetylase and plays an essential role in caloric restriction, life span modulation, and cell fate determination.Recently, a number of studies have demonstrated that SIRT1 plays a protective role in colitis.

Research motivation

Although a large number of therapeutic agents, including 5-ASA drugs, immunosuppressants,steroids, and emerging biological agents, have appeared in the past few years, most patients still experience severe complications or recurrence of the disease, which greatly reduces their quality of life.

Research objectives

To investigate the role of SIRT1 in intestinal epithelial cells in UC and further explore the underlying mechanisms.

Research methods

We developed a coculture model using macrophages and Caco-2 cells. After treatment with the SIRT1 activator SRT1720 or inhibitor nicotinamide (NAM), the expression of occludin and zona occludens 1 (ZO-1) was assessed by Western blot. Annexin V-APC/7-AAD assays were performed to evaluate Caco-2 apoptosis. DSS-induced colitis mice was exposed to SRT1720 or NAM for 7 d. Transferase-mediated dUTP nick-end labeling (TUNEL) assays were conducted to assess apoptosis in colon tissues. The expression levels of glucose-regulated protein 78 (GRP78),CCAAT/enhancer-binding protein homologous protein (CHOP), caspase-12, caspase-9, and caspase-3 in Caco-2 cells and the colon tissues of treated mice were examined by quantitative real-time PCR and Western blot.

Research results

SRT1720 treatment increased the protein levels of occludin and ZO-1 and inhibited Caco-2 apoptosis, whereas NAM administration caused the opposite effects. DSS-induced colitis mice treated with SRT1720 had a lower disease activity index (P ＜ 0.01), histological score (P ＜ 0.001),inflammatory cytokine levels (P ＜ 0.01), and apoptotic cell rates (P ＜ 0.01), while exposure to NAM caused the opposite effects. Moreover, SIRT1 activation reduced the expression levels of GRP78, CHOP, cleaved caspase-12, cleaved caspase-9, and cleaved caspase-3 in Caco-2 cells and the colon tissues of treated mice.

Research conclusions

SIRT1 activation contributes to enhanced intestinal barrier integrity and reduced apoptosis of intestinal epithelial cells via the suppression of endoplasmic reticulum (ER) stress-mediated apoptosis-associated molecules CHOP and caspase-12.

Research perspectives

SIRT1 may serve as a novel drug target, and SIRT1 activation is a promising therapeutic strategy for UC.

We are grateful to Dr. Wei-Xiang Zhong for excellent assistance in pathology.