高粘度快速發(fā)酵生產(chǎn)燃料乙醇技術(shù)研究最終報告

靳艷玲 趙海 方揚

摘 要：在粘度產(chǎn)生機制方面，以甘薯為模式原料利用多糖單克隆抗體芯片分析技術(shù)解析了粘度產(chǎn)生的機制，為降粘技術(shù)的開發(fā)提供了靶點；在降粘技術(shù)方面，利用商品化酶開發(fā)了復(fù)合降粘酶系，同時還自主篩選出了可以同時產(chǎn)生復(fù)合降粘酶的單菌株，并開發(fā)了配套的降粘工藝，可使甘薯、木薯、芭蕉芋等粘度下降90%以上；在菌種選育方面，選育到8株高濃度乙醇發(fā)酵酵母，并通過適應(yīng)性馴化提高了菌株對高溫、高壓等環(huán)境壓力條件的抗性，同時還研究了菌株的壓力耐受機制，為進一步改造或優(yōu)化其應(yīng)用工藝提供數(shù)據(jù)支持；在發(fā)酵過程調(diào)控方面，開發(fā)出了代謝促進劑及配套的高濃度快速乙醇發(fā)酵技術(shù)，發(fā)酵24 h內(nèi)，乙醇濃度可達12%以上；在反應(yīng)器開發(fā)方面，開發(fā)出新型利用變頻泵驅(qū)動的高傳質(zhì)低能耗反應(yīng)器，可使粘度高達9萬mPa·S的薯類濃醪傳質(zhì)均勻；通過高效乙醇發(fā)酵菌株，降粘技術(shù)體系，快速乙醇發(fā)酵和高濃度乙醇發(fā)酵等關(guān)鍵技術(shù)模塊的系統(tǒng)集成建立了高粘度快速發(fā)酵生產(chǎn)燃料乙醇技術(shù)體系，并應(yīng)用于西南地區(qū)最大的乙醇生產(chǎn)企業(yè)——資中縣銀山鴻展工業(yè)有限責(zé)任公司3萬t燃料乙醇示范生產(chǎn)線上。以鮮甘薯為原料，發(fā)酵時間由現(xiàn)有技術(shù)的60 h以上縮短為30 h以內(nèi)，乙醇濃度由5%～6%（v/v）平均提高到10.69%（v/v），最高可達12.41%（v/v），發(fā)酵效率由88%以下提高至90%以上，可提高單位設(shè)備的生產(chǎn)力，降低乙醇蒸餾能耗，節(jié)能效果明顯。

關(guān)鍵詞：燃料乙醇 粘度 非糧

Abstract：In terms of the mechanism of viscosity， sweet potato was used as a model material and investigated with polysaccharide monoclonal antibody microarray. The results of this analysis explained what kinds of component related with viscosity and provided targets for viscosity reduction technology. Complex enzymes was developed for viscosity reduction， and fungi which could produce complex viscosity reduction enzymes was screened. Under the function of these enzymes， viscosity of sweet potato，cassava and canna was reduced by 90%. In terms of microbe， 8 strains of yeast were screened， and then acclimation to high temperature and high concentration of ethanol was carried out. The mechanism of stress tolerance of these yeasts was investigated， and the results would be benefit to better performance of these yeasts. In terms of fermentation technology， one fermentation stimulant was developed. Under the function of this stimulant，12% ethanol could be produced within 24h. In terms of reactor， novel reactor with high performance and low energy consumption was developed， which could blend high viscosity sweet potato mash well. After system integration， these technologies were applied to 30，000t scale of production line in the biggest ethanol plants in southwest of China. Fermentation time was reduced from more than 60h to less than 30h， ethanol concentration was increased from 5%～6% to 12%， and ferment efficiency was enhanced from 88% to 90%. These changes of parameters could enhance productivity of facilities， and decrease energy consumption significantly.

Key Words：Fuel Ethanol；Viscosity；Non-grain Material

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