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    Phenotypic Traits and Genetic Diversity of Erianthus arundinaceum Germplasm

    2015-12-12 06:48:18ChaohuaXUXinLULiMAXinlongLIUHongboLIUHuoshengSUXiuqinLINQingCAI
    Agricultural Science & Technology 2015年1期
    關(guān)鍵詞:甘蔗表型種質(zhì)

    Chaohua XU, Xin LU, Li MA, Xinlong LIU, Hongbo LIU, Huosheng SU, Xiuqin LIN,Qing CAI,3*

    1. Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences, Kaiyuan 661699, China;

    2. Yunnan Key Laboratory of Sugarcane Genetic Improvement, Kaiyuan 661699, China;

    3. Biotechnology & Genetic Resources Institute, Yunnan Academy of Agriculture Sciences, Kunming 650223, China

    Sugarcane (Saccharum L) as the major sugar crop contributes to 90% of sugar production in China[1]. At present, sugarcane varieties worldwide are mostly the hybrids of tropical species Saccharum officinarum, S. spontaneum and Indian species S. barberi, so they have similar genetic background,which limits the breeding and promotion of new varieties[2].Erianthus arundinaceum, a species belonging to an allied genus of sugarcane, widely distributed in Yunnan, Sichuan, Guizhou,Fujian,Hainan and Guangxi and other provinces of China, has excellent traits, such as good tillering ability,adaptability, drought tolerance and abundant ecological types[3-4]. In recent years increasing attention has been paid to the collection of E.arundinaceum resources[5-8], and their genetic diversity had been evaluated through morphological markers[9], cytological markers[10-12], protein markers[13-14]and mo-lecular markers[15-17],etc.Besides, extensive studies have been carried out on their tolerance to cold[18], drought[19-21]and disease[22]. In this study, the genetic diversity of phenotypic traits of 162 accessions of E. arundinaceum fromnine provinces of China was investigated to explore their genetic background,with an attempt to provide theoretical references for resource collection , heterosis application and development of excellent genes of sugarcane germplasm.

    Materials and Methods

    Materials

    The 162 accessions of E.arundinaceum were preserved in the National Nursery of Sugarcane Germplasm Resources, among which, 74 samples were collected from Yunnan,15 from Fujian, 19 from Guizhou, 18 from Hainan,14 from Sichuan,10 from Jiangxi, 4 from Guangdong, 4 from Guangxi and 4 from Zhejiang.

    Measurement items and methods

    The experiment was conducted at the National Nursery of Sugarcane Germplasm Resources in November 2008 . All the 162 accessions of E. arundinaceum were separately grown in cement frames (0.8 m in diameter and depth),spacing 1 m to each other. Six normal plants from each accession were selected and surveyed for five quantitative traits and 21 qualitative traits. The five quantitative traits were lamina length,lamina width,stalk length, stalk diameter and brix,and the 21 qualitative traits were aerial root, stalk shape, pipe, pith, internode shape,internode color unexposed and exposed, growth ring shape, root primordial, bud shape, bud placement,sheath detached from culm, hair group,shape of inner auricle,shape of outer auricle, angle of lamina to culm,lamina colour, wax band, corky patch and growth crack. From which, ten of the phenotypic traits with diversity:internode color unexposed, internode color exposed,growth ring shape,root primordial,bud shape,bud placement,hair group, angle of lamina to culm,lamina colour, wax band were screened out and analyzed for their genetic diversity. All the data were standardized according to the literatures[23]and[24]. The quantitative traits were grouped into six levels for cluster analysis together with the qualitative traits.

    The genetic diversity of the populations was measured by Shannon-Wiener index which was calculated with the formula as follows:

    Wherein, H’ reflects the genetic diversity of the populations; Piis the frequency of the i-th code value of a quality trait[25].The total genetic diversity(Ht),the genetic diversity within populations(Hs),genetic divergence coefficient (Gst) and gene flow value (Nm)were all calculated using the formulas described in literature[26].

    Data analysis

    Spss17.0 software was adopted to analyze the coefficient of variations(CV) and degree of dispersion of the five quantitative traits. Excel and NTSYSpc2.1 software were used to calculate the phenotypic frequencies of qualitative traits, and the Nei’s genetic distance between the populations from different provinces. Finally, a dendrogram was constructed from Nei’s genetic distance based on UPGMA(weighted pair group method using arithmetic averages).

    Results and Analysis

    Coefficient of variation in the quantitative traits

    As shown in Table 1, among the five quantitative traits of the 162 E. arundinaceum accessions, the average coefficient of variation for lamina width was the largest (36.48%),followed by that for stalk length(20.33%)and lamina length (19.71%), while the coefficient of variation for brix(11.65%)was the smallest. Therefore, quantitative traits should be considered as an important index for the assessment of E. arundinaceum resources.

    Besides, by comparing the variation of the five quantitative traits for the E. arundinaceum samples from different regions,it was found that the population from Fujian had the largest variation coefficient of stalk length(30.91%), while the population from Guangxi had the smallest one(11.53%); the population from Fujian also had the largest variation coefficient of stalk diameter (26.68%), while the population Zhejiang had the smallest one (8.63%); the largest variation coefficient of brix (20.92%)was found in population from Yunnan, and the smallest (7.59%) in population from Guangxi;the largest coefficient of variation for stalk length (61.96%) was found in population from Yunnan, and the smallest (4.54% ) in population from Jiangxi; the largest coefficient of variation for stalk width (67.99%)was found in population from Fujian, and the smallest (10.97%) in population from Zhejiang.

    The coefficients of variation for the five quantitative traits of the samples in each province were averaged,and the resulting means ranged from 14.95%to 32.14,as shown in Table 1.Among them, the mean in Yunnan was the largest (32.15%), followed by that Fujian (30.09%)and Guangdong(26.23%), while that in Guangxi was the smallest(14.95%).

    Table 1 Coefficients of variance of quantitative traits for Erianthus arundinaceum populations from different sampling regions %

    Genetic diversity in quality traits

    As could be seen from Table 2,the Shannon-Wiener indices for the 10 qualitative traits of the E. arundinaceum samples from each province were averaged, and the resulting means ranged from 0.294 2 0.762 4,which were close to that of the decaploids of Saccharum spontaneum,but lower than the Shannon-Wienerindices of the sugarcane germplasm[27-28].Among the nine provinces,the Shannon-Wiener index in Fujian was the largest (0.762 4), followed by that in Guizhou(0.701 9),Sichuan(0.613 9)and Yunnan Province (0.601 5), while that in Guangxi was the lowest(0.294 2).In addition, by comparing the Shannon-Wiener indices of the 10 qualitative traits of the 162 E. arundinaceum accessions, it was found that bud placement had the largest Shannon-Wiener index (0.839 2), followed by lamina colour (0.635 4), and root primordial had the smallest diversity index(0.354 7).

    Table 2 Shannon-Wiener index of qualitative trait for Erianthus arundinaceum populations from different sampling regions

    Correlations of quantitative traits with latitude and altitude

    It could be concluded from Table 3 that altitude had an extremely significant negative correlation with brix,but no significant correlations with stalk length, stalk diameter, lamina length and lamina width of E. arundinaceum samples, indicating that brix deceased with the increase in altitude. Latitude had an extremely significant negative correlation with stalk length, but no significant correlations with stalk diameter, lamina length, lamina width and brix of E.arundinaceum samples, indicating that their stalk length increased with the increase of latitude. Therefore, E. arundinaceum resources with large plant and high brix could be easily found in low-latitude and low-altitude areas, and those with short plant and low brix in high-latitude and high-altitude areas.

    Genetic diversity of E. arundinaceum populations from different provinces

    The genetic diversity index for the quantitative traits within E. arundinaceum populations from the nine provinces was 0.629 6, and the total genetic diversity index was 0.725 4.The genetic diversity index for their quality traits within the populations was 0.177 1, and the total genetic diversity was 0.232 8. The results suggested that the quantitative traits of these E. arundinaceum resources had richer genetic diversity than their quality traits. For quality traits, the genetic divergence coefficient was 23.95% and gene flow was 1.587 9;for quantitative traits, the genetic divergence coefficient was 13.20%, and the gene flow was 3.287 8, suggesting that there was active genetic exchanges and no obvious genetic divergence among the E. arundinaceum populations from different regions.

    Cluster analysis of the E. arundinaceum populations from different regions

    The Nei’s genetic distances between the E. arundinaceum populations from different regions were relative small, ranging from 0.083 4 to 0.513 7, with an average value of 0.227 2,indicating a low degree of genetic divergence among the populations. The genetic distance between the populations from Jiangxi and Hainan (0.083 4) was the smallest,and that between the populations from Sichuan and Jiangxi (0.097 5)was the second smallest; the genetic distance between the populations from Guangxi and Zhejiang was the largest (0.513 7),followed by that between the populations from Zhejiang and Hainan(0.497 8).

    As shown in Fig.1, the E. arundinaceum population from Zhejiang was most distantly related to the populations from other regions, followed by that from Guangdong and Guangxi.The populations from Jiangxi and Hainan were most closely related and clustered together, indicating that they had the most similar genetic background, and then they clustered with the populations from Sichuan, Fujian,Guizhou and Yunnan. All the results showed that the phenotypes of the E. arundinaceum samples in this study had a certain relationship with their geographical locations.

    Table 3 Correlation coefficients between quantitative traits and latitude or altitude

    Table 4 Genetic structure of Erianthus arundinaceum populations from different sampling regions

    Conclusion and Discussion

    Liu et al.[28]studied the genetic diversity of 1 160 sugarcane accessions from 20 regions of 13 countries, and provided useful references for the selection of parental materials, hybrid combinations and construction of core collection for sugarcane breeding.The study of Xiao et al.[29]which was about genetic diversity in morphological traits of Miscanthus floridulus provided theoretical information for the breeding and genetic improvement of Chinese M. floridulus resources. The study of Liu et al.[27]discovered the diverse Saccharum spontaneum resources in China. Our findings proved that abundant genetic variation existed in the morphological traits of E.arundinaceum populations from different regions. The variation coefficients of the five morphological traits (such as stalk length and stalk diameter) among the E. arundinaceum populations from the nine provinces ranged from 1.65% to 36.48%, with an average of 20.90%,indicating the great genetic differences and abundant genetic diversity of them.Besides,the quantitative traits of the E. arundinaceum populations shared significant correlations with latitude and altitude. Among them, brix had an extremely significant negative correlation with altitude, while stalk length had an extremely significant negative correlation with latitude. UPGMA cluster analysis showed that the morphological traits of the E. arundinaceum populations had certain relationship with their location, indicating that they were affected by geographical environments.

    The genetic divergence coefficient for quality traits among the E. arundinaceum populations from different regions was 23.95%, indicating that only 23.95% of the genetic variation was caused by their geographical locations, while 76.05% of the variation was retained within the populations. The genetic divergence coefficient for quantitative traits among the E. arundinaceum populations was 13.20%, indicating that 86.8% of the variation was retained within populations. In summary, extensive genetic exchanges existed in both the quality and quantitative traits of these E. arundinaceum populations, while the gene flow of quantitative traits was over two folds of qualitative traits, indicating that there should be more emphasis on quantitative traits than quality traits in resource evaluation of E. arundinaceum.The large gene flow also indicated a low degree of genetic divergence, which may be related to the propagation and selection of E. arundinaceum under natural environmental conditions[30].

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