Hibiscus sabdariffa extract inhibits in vitro biofilm formation capacity of Candida albicans isolated from recurrent urinary tract infections

Issam Alshami, Ahmed E Alharbi

Department of Medical Microbiology and Immunology, College of Medicine, Taibah University, Almadinah, Saudi Arabia

Hibiscus sabdariffa extract inhibits in vitro biofilm formation capacity of Candida albicans isolated from recurrent urinary tract infections

Issam Alshami*, Ahmed E Alharbi

Department of Medical Microbiology and Immunology, College of Medicine, Taibah University, Almadinah, Saudi Arabia

PEER REVIEW

Peer reviewer

Dr. Tarek Girbeel, Research Scientist, University of Manchester School of Translational Medicine, Stopford Building, Oxford Road, Manchester, M13 9PL.

E-mail: tarek.gibreel@manchester. ac.uk

Comments

Generally the presentation of the paper is good. Methodology is appropriate and the results are presented properly. Based on the results, the authors reported a new application of this valuable medicinal plant. The results of biofilm inhibition indicate that this plant can be used to prevent recurrent UTIs.

Details on Page 107

Objective:To explore the prevention of recurrent candiduria using natural based approaches and to study the antimicrobial effect of Hibiscus sabdariffa (H. sabdariffa) extract and the biofilm forming capacity of Candida albicans strains in the present of the H. sabdariffa extract.

Hibiscus sabdariffa, Biofilm, Candiduria, Candida albicans, UTIs

1. Introduction

Candida albicans(C. albicans) is commensal yeast normally present in small numbers as a skin, oral, gut or vaginal flora and is found in 15%-60% of the population.C. albicansis traditionally regarded as a low grade pathogen. A common form of candidiasis that is restricted to the mucosal membranes in the mouth or vagina known as thrush, which is usually easily treated[1]. Systemic candidal infections have emerged as important causes of morbidity and mortality in immunocompromised patients[2]. The emergence of fungal urinary tract infections (UTIs) poses an interesting diagnostic and therapeutic challenge. During the past decade, there has been a worldwide trend in increasing occurrences of fungal UTIs.C. albicanswas reported as the most common cause of fungal UTIs (candiduria)[3]. Candiduria may be resolved spontaneously, but may also result in serious complications if treated inadequately, and recurrent or persistent infections are frequent. Many risk factors for candiduria found including urinary system intervention, catheter use and immunosuppression history. There are other risk factors for candiduria including older age, sex, antibiotic exposure and diabetes[4].

Candiduria has raised alarms with the global infectiousdiseases community because of the limited therapeutic options. The limited success over the past decade of prevention and control candiduria highlights the difficulty of limiting the problem once it has already been established[5]. In contrast to antibacterial agents, antifungal agents have less of a clinical benefit. This is due to the higher level of toxicity to human cells because both fungi and the host cells are eukaryotic and share similar cellular structures. In recurrent candiduria, long-term antifungals are administered to the patients and this is generally unfavourable from the points of medical cost and the potential development of fungal resistance[6].

Current pharmacologic treatment options for symptomatic candiduria include amphotericin B, flucytosine, or azole. Due to its ease of administration and pharmacokinetic profile, fluconazole has emerged as the most commonly used drug to treat candiduria. In addition, fluconazole’s adverse effect profile may be more tolerable than the complications associated with other antifungal agents. However, the failure of fluconazole treatments may be associated with either someCandidaspecies innate resistance or due to an acquired resistance to the drug. The emergence of fluconazole resistance inC. albicanswas observed after reports showed that the long-term use of fluconazole for prophylaxis lead to the emergence of resistance[7].

Therefore, promoting the application of natural plantderived antimicrobials as a possible approach to prevent recurrent candiduria is very desirable and the potential effect of natural options for long-term prevention of infections should be explored. Hence, in this study we examined thein vitroantimicrobial activities ofHibiscus sabdariffa(H. sabdariffa) extract against fluconazole resistantC. albicansisolated from recurrent candiduria.H. sabdariffais a common herbal drink consumed both hot and cold by people around the world and used in traditional medicine in the treatment of hypertension. The infusion is made from the calyces of theH. sabdariffa[8]. Relatively few studies have been carried out to evaluate the antimicrobial activities of this plant against common human pathogens[9,10]. However, no study has been reported concerning the effect of the plant in biofilm forming uropathogenicC. albicansand the combination effect ofH. sabdariffawith other antifungal drugs. The aim of this study was to assess the antifungal activity ofH. sabdariffaextract againstC. albicansfrom recurrent candiduria in order to better understand their therapeutic properties.

2. Materials and methods

2.1. Preparation of plant extracts

Air driedHibiscus calyceswere purchased from the local market. The identity of the plant was confirmed by comparing collected voucher specimen with those of a known identity located in the Herbarium of the College of Science, Taibah University. A voucher specimen was deposited at the Herbarium under the reference number 1433/14. The extracts were prepared according to a previously described protocol[11]. Using 1 L of 80% aqueous methanol (BDH, UK), the phenolics were extracted from 100 g of the grinded calyces. The mixture was then sonicated for 20 min and then filtered through Whatman No. 2 filter paper. The solvents were then evaporated using a rotary evaporator at 40 °C under reduced pressure and the extracts were stored in tightly, sealed glass vials and considered to beH. sabdariffaextract, and was used for the antibacterial assays. Dried plant extracts were then dissolved in methanol to give a stock solution of 500 mg/mL and then filter sterilized before use.

2.2. Fungal strains and growth conditions

In this particular study, six strains of fluconazole resistantC. albicans, CA1, CA2, CA3, CA4, CA5 and CA6 were used. All were obtained from patients with recurrent candiduria which had been isolated from Ohad Hospital, Almadinah, Saudi Arabia. Fungal identities were confirmed using standard microbiologic procedures and the use of API ID 32C strips (Biomerieux, France).

2.3. Determination of antimicrobial activity

The fluconazole andH. sabdariffaextracts minimum inhibitory concentrations (MICs) and time-kill curves were determined according to the approved CLSI standard reference method for broth dilution and antifungal susceptibility testing of yeasts M27-A3[12]. MIC plates were incubated at 35 °C for 48 h. MICs were defined as the lowest concentration of the drug eliminating cell growth using MIC-0. MIC-0 was defined as the lowest drug combination resulting in optically clear wells.

2.4. Effect on C. albicans biofilm formation

The ability to form aCandidabiofilm was tested on 96-well tissue culture plates and biofilm was quantified by measuring the optical density after dyeing it with 0.1% crystal violet in accordance to a previously described protocol[13]. This method measures the cells adhering to the bottom of microtiter plate wells after repetitive cycles of washing. Briefly, strains from an overnight culture were inoculated with the tested organism in the presence and absence of sub-MIC concentrations of the extract at 37 °C without agitation for 48 h in yeast nitrogen base broth +0.5% glucose. The attached cells were washed, fixed and stained with crystal violet and air dried. About 0.15 mL of95% ethanol was added in each well and the plates were incubated for 10 min. Biofilm formation was quantified by measuring the absorbance at 550 nm. Each strain and each concentration were assayed in three wells on each plate, and all experiments were replicated at least three times.

2.5. Statistical analysis

Data was analysed in SPSS statistical software.P＜0.05 was considered to be statistically significant when compared with control.

3. Results

3.1. Antifungal activities

Various levels MIC ofH. sabdariffaextract were observed against all the isolates. MICs values ranged from 0.5 to 2.0 mg/mL. All the isolates showed resistance to fluconazole with MICs values of ＞16 μg/mL (P＜0.05). The results are summarized in Table 1.

Table 1 Antifungal activities of H. sabdariffa extract and fluconazole against C. albicans.

3.2. Mode of action

Time-kill curves were performed for the isolates using different concentrations of theH. sabdariffaextract ranging from 0.5-2× the MIC value. The results obtained for the time-kill curves are summarized in Figure 1. For the six strains, the effects were fungistatic (P＜0.05). Fungistatic activity has been defined as ＜3 log reduction in CFU/mL using time-kill[14]. The present study allows us to establish thatH. sabdariffahas a fungistatic effect against theC. albicansisolates.

Figure 1. Time-kill experiments evaluating the activity of H. sabdariffa extract against C. albicans isolates.Fungistatic activity was defined as a reduction in the numbers of viable bacteria of ＜3 log10CFU/mL at any of the incubation times tested. P＜0.05, between growth control and different concentrations of the extracts; ×: Minimum inhibitory concentration of the extract; CA: C. albicans.

3.3. Inhibition of biofilm formation by H. sabdariffa

There is no previous data that suggestsH. sabdariffamay inhibit biofilm formation against candiduria isolates. Thus, the current study was initiated to evaluateH. sabdariffaextracts on those pathogens that are clinically relevant. The results showed that theH. sabdariffaextract inhibited biofilm production on all of the isolates. The biofilm inhibition varies between the different isolates and ranged from 33%-55% (P＜0.05). The results obtained for the biofilm formation assays are summarized in Figure 2.

Figure 2. Inhibition of biofilm formation in microtiter plates of C. albicans isolates by H. sabdariffa extract.All strains showed reduced biofilm formation in the presence of different concentrations of H. sabdariffa extract. Biofilm formation was determined by crystal violet staining. Values are means of three independent experiments. P＜0.05, between growth control and different concentrations of the extracts. X: Minimum inhibitory concentration of the extract, OD: Optical density, CA: C. albicans.

4. Discussion

Few studies have been carried out to evaluate the antimicrobial activities ofH. sabdariffaextract against common bacterial pathogens. Extracts of the plant significantly enhanced the inhibitory effects of antibacterial agents against different bacterial species[15]. However, there has been only one study reported the effect of the plant extract against fungal pathogens.H. sabdariffawas found to be effective at all levels in inhibitingC. albicans(non candiduria isolates) but no otherCandidaspecies[9]. Previous studies have shown this effect in terms of inhibition zones or agar dilution methods. In our study, we chose the most appropriate and reliable method for antimicrobial susceptibility testing (i.e., the MIC using microdilution method). The advantage of this technique was that it generated a quantitative result in which with further analysis provided a clearer interpretation of the results in terms of time-kill. The present study allows us to establish, for the first time, the effect ofH. sabdariffaextract againstC. albicansisolated from recurrent UTIs.

The high potency ofH. sabdariffaagainst these strains gives scientific basis for its use in folk medicine for treating and preventing of UTI. The mechanism of the action is not fully understood but it has been suggested thatH. sabdariffaproducts contain phenolic compounds including flavonoids and cyanidin[16,17]. Phenolic compounds have been reported to exhibit antimicrobial activities[11,18,19,]. The antimicrobial effect of the phenolic compounds may be due to iron deprivation or hydrogen bonding with vital proteins such as microbial enzymes[20].

Nearly allCandidaspecies strain possess different ranges of virulence factors, including the use of several adherence mechanisms to colonize uroepithelium of both the upper and lower urinary tract. Biofilm forming uropathogens are often associated with long term persistence and display a dramatically increased resistance to antimicrobial agents. Agents interfering with biofilm formation represent a novel approach to controlC. albicansinfections. By affectingC. albicansvirulence properties, this may minimize the appearance of resistant strains[21,22].

H. sabdariffacontains a unique polymeric compound known as proanthocyanidin. Research shows that proanthocyanidin from cranberry extracts inhibit the biofilm formation ofC. albicansisolated from oral infection[13]. The present study suggests that proanthocyanidin fromH. sabdariffahas the same anti-biofilm inhibition effect againstC. albicansstrains isolated from UTIs.

The use of plant extracts and phytochemicals with known antimicrobial properties is becoming a very common practice worldwide and is of great significance in therapeutic use. The wide extensive use of antifungal agents leads to the selection of clinical isolates that show multidrug resistance. On the other hand, few synthetic antifungals agents are being developed. The identification of plant extracts with antimicrobial properties would be of an immense practical use as a safe and effective means of controlling clinical infections[23,24].

In conclusion, the current study shows thatH. sabdariffaextract significantly inhibits theC. albicansgrowth and prevents the formation ofin vitrobiofilm. The antifungal activities of the extract that were observed provide basic evidence for the potential effects of this plant for preventing recurrent candiduria caused byC. albicans. It also emphasizes a significant approach of the plants extract as an antimicrobial agent. Although a preventive effect ofH. sabdariffahas been shownin vitro, further studies with a more robust methodology would be needed in order to determine thein vivoclinical efficiency of the extract to prevent recurrent UTIs.

Conflict of interest statement

We declare that we have no conflict of interest.

Acknowledgements

The authors are grateful to Mr. Mohamed Abdulsamad and all the staff in the Department of Medical Microbiology, Ohad Hospital who made a great effort to collect and identify the fungal isolates. We acknowledge the Deanship of Scientific Research of Taibah University for providing funding for this research (Grant No.432/3088).

Comments

Background

H. sabdariffa, commonly known as karkade, grows and is cultivated in different parts of the world such as Africa and Asia. The calyces parts of plant is used in traditional medicine in the treatment of hypertension. Many research studies have been carried out to validate its traditional uses.

Research frontiers

Current article aimed to evaluate and compare the antifungal potential and activity of the methanolic extract againstC. albicansisolated from candiduria. The authors also examined the biofilm inhibition activity of the extract. This paper describes for the first time the extract effect against fungal isolates from candiduria.

Related reports

Relatively, few studies have been carried out to evaluate the antimicrobial activities of this plant against common human pathogens (Darwishet al., 2010). This study focuses on the antifungal effect ofH. sabdariffaextract againstC. albicansisolated from recurrent UTIs.

Innovations and breakthroughs

This study has shown thatH. sabdariffaextract can be further developed for use in the treatment of infectious diseases.

Applications

This report finds wide applications in medicine.H. sabdariffaextract may be used as a local source of antifungal agents to treat and prevent UTIs.

Peer review

Generally the presentation of the paper is good. Methodology is appropriate and the results are presented properly. Based on the results, the authors reported a new application of this valuable medicinal plant. The results of biofilm inhibition indicate that this plant can be used to prevent recurrent UTIs.

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10.1016/S2221-1691(14)60217-3

*Corresponding author: Dr Issam Alshami, Department of Medical Microbiology and Immunology, College of Medicine, Taibah University, Almadinah, Saudi Arabia.

Tel: +966 581322214

E-mail: issam.alshami-2@manchester.ac.uk

Foundation Project: Supported by the Deanship of Scientific Research of Taibah University (Grant No.432/3088).

Article history:

Received 26 Oct 2013

Received in revised form 4 Nov, 2nd revised form 14 Nov, 3rd revised form 23 Nov 2013

Accepted 22 Dec 2013

Available online 28 Feb 2014

Methods:In this particular study, six strains of fluconazole resistant Candida albicans isolated from recurrent candiduria were used. The susceptibility of fungal isolates, time-kill curves and biofilm forming capacity in the present of the H. sabdariffa extract were determined.

Results:Various levels minimum inhibitory concentration of the extract were observed against all the isolates. Minimum inhibitory concentration values ranged from 0.5 to 2.0 mg/mL. Timekill experiment demonstrated that the effect was fungistatic. The biofilm inhibition assay results showed that H. sabdariffa extract inhibited biofilm production of all the isolates.

Conclusions:The results of the study support the potential effect of H. sabdariffa extract for preventing recurrent candiduria and emphasize the significance of the plant extract approach as a potential antifungal agent.