Phytochemical screening and antioxidant activity of ethanol extract of Tithonia diversifolia (Hemsl) A. Gray dry flowers

Robson Miranda da Gama, Marcelo Guimar?es, Luiz Carlos de Abreu, José Armando-Junior*

1Laboratório de Pesquisa do Curso de Farmácia, Faculdade de Medicina do ABC, Avenida Príncipe de Gales, 821, Vila Príncipe de Gales, 09060-870, Santo André, SP, Brasil

2Departamento de Saúde Materno-Infantil, Faculdade de Saúde Pública, Universidade de S?o Paulo, Brasil

Phytochemical screening and antioxidant activity of ethanol extract of Tithonia diversifolia (Hemsl) A. Gray dry flowers

Robson Miranda da Gama1, Marcelo Guimar?es1, Luiz Carlos de Abreu2, José Armando-Junior1*

1Laboratório de Pesquisa do Curso de Farmácia, Faculdade de Medicina do ABC, Avenida Príncipe de Gales, 821, Vila Príncipe de Gales, 09060-870, Santo André, SP, Brasil

2Departamento de Saúde Materno-Infantil, Faculdade de Saúde Pública, Universidade de S?o Paulo, Brasil

ARTICLE INFO

Article history:

Received 28 Jan 2014

Received in revised form 20 Mar 2014

Accepted 24 Apr 2014

Available online 28 Jun 2014

Antioxidant activity

Objective:To evaluate the antioxidant activity of extracts of dried flowers of Tithonia diversifolia (Hemsl) A. Gray (T. diversifolia) dry flower-a shrubby plant belonging to the Asteraceae family and very common in Brazil, providing data to help prevent premature aging skin.

1. Introduction

Skin aging is commonly influenced by several factors, such as genetic and environmental factors (UV light), xenobiotics, and hormonal changes. All these factors can trigger the onset of reactive oxygen species (ROS) that are chemically reactive molecules containing oxygen[1].

They are formed as natural products of the normal metabolism of oxygen and have important roles in cell signaling and homeostasis. However, during times of environmental stress, ROS levels can dramatically increase that, resulting in significant damage to cell structure[2].

Natural antioxidants present in plant origin protect against these radicals and are therefore important tools in obtaining and preserving good health[3].

Potentially active components from fruits, herbs, roots and leaves have been studied extensively in order to avoid oxidative cellular events. The results suggest that polyphenols, especially the flavonoids possess a high antioxidant power which can protect cells against the adverse effects of ROS[4].

Among many medicinal plant families, Asteraceae family comprises species with arboreous, shrub, herbaceous and liana habits and is widely distributed at tropical, subtropical and tempered regions, particularly in South America, with expression in number of species, composed by some 1 535 genera, 23 000 species and 17 tribes[5].

Tithonia diversifolia(Hemsl) A. Gray (T. diversifolia) is a herb family (tribe Heliantheae) occurring from Central America to the West Indies, having been naturalized in the tropics and also has been used as a medicinal plant showing their anti-inflamatory[6], antimalarial[7] and many other biological activities.

The aims of the present study were to evaluate phytochemical screening and also measure the antioxidant activity of ethanol extract ofT. diversifoliadry flowers using a 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assay.

2. Materials and methods

2.1. Botanic material

Plants ofT. diversifoliawere grown in the Garden ofMedicinal Plants, Faculty of Medicine of ABC, Santo André, S?o Paulo, Brazil, and flowers were collected in August 2012 (flowering season of this species). After manual collect, the material was dried at 50 °C (hot air chamber) for 1 week and stored under controlled conditions (dry air, dark and at 20 °C).

2.2. Extract preparation

For extract preparation, the flowers were reduced in a mill and kept in ethanol (100%) under stirring at room temperature for 24 h. After filtering, the extract was concentrated on the rotary evaporator attached to a vacuum pump and then used for phytochemical screening.

2.3. Phytochemical screening

All the tests of phytochemical screening (total phenols, tannins, flavonoids, alkaloids and saponins) followed the methodologies described at Brazilian Pharmacopea[8].

2.4. Antioxidant activity

The DPPH method is based on captures of DPPH by antioxidants, producing a decrease in absorbance at 517 nm.

Plant sample solutions (0.1 g/mL) were diluted to final concentrations of 250, 100, 50 and 10 mg/mL, in ethanol. Ascorbic acid solutions (1.8 mg/mL) were diluted to final concentrations of 120, 90, 60 and 30 μg/mL, in ethanol.

About 3 mL of a 0.04 mg/mL DPPH (Sigma AldrichTM) ethanol solution was added to 30 μL of sample solutions of different concentrations, and allowed to react at room temperature. After 30 min, the absorbance values were measured at 517 nm and converted into percentage antioxidant (AA) using the following equation 1 (Eq. 1): Eq. 1: AA(%)=100-{[(Abssample-Absblank)×100]/Abscontrol}

Ethanol (3.0 mL) plus plant extract solution (30 μL) was used as a blank. DPPH solution (3.0 mL; 0.04 mg/mL) plus ethanol (3.0 mL) was used as a negative control. The positive control was those using the standard ascorbic acid (FlukaTM) solutions.

The calculation equations of the analytical curves were made by linear regression using the least squares method of plots where the abscissa represented the concentration of test plant extracts or ascorbic acid and the ordinate represented the average percent of antioxidant activity and calculating the linear correlation coefficient. The equation 2 (Eq. 2) and equation 3 (Eq. 3) used to calculate the results of percent of antioxidant activity of ethanol extract ofT. diversifoliadry flowers and ascorbic acid reference standard, respectively.

Eq. 2:y=0.236x+1.421 5R2=0.997 8

Eq. 3:y=0.621 2x+3.015R2=0.995 9

The half maximal inhibitory concentration (IC50) is a measure of the effectiveness of a compound in inhibiting antioxidant activity. The IC50values of ethanol extract ofT. diversifoliadry flowers and ascorbic acid reference standard were calculated by Eq. 2 and Eq. 3, respectively[9].

3. Results

3.1. Phytochemical screening

The phytochemical screening test showed that the flowers ofT. diversifoliacontained several active compounds (Table 1). The IC50of dried flower of ethanol extracts of this species and ascorbic acid are presented in Figure 1.

Table 1 Phytochemical composition of ethanol extract of T. diversifolia dry flowers.

Figure 1. The IC50values (the half maximal inhibitory concentration) of ethanol extract of T. diversifolia dry flowers and ascorbic acid.

4. Discussion

In this study, the phytochemical screening of flower extract ofT. diversifoliarevealed that among the substances investigated, presence of phenolic compounds was detected (total phenols, tannins and flavonoids), while alkaloids and saponins were not detected. The presence of some of these secondary metabolites suggests that the plant might be of medicinal importance.

The presence of phenolic compounds (total phenols, tannins and flavonoids) provides pharmacological activities like anti-cancer[10,11], anti-oxidant[11,12], antimicrobial[13,14], wound-healing[15] and anti-inflammatory[6,16], that may suggest an association to the species here investigated.

Similar results of phytochemical screening of flower extract of this species were obtained by Essiett and Akpan[17], differing only in the saponin presence and the result may be related to the parts of the flower used to obtain the extract in the study by Essiett and Akpan that were used only the petals of flowers[17], while in present study were obtained by the whole flower.

The theory of aging skin by the action of free radicals is based on the failure mechanism of natural antioxidantin vivoandin vitrosince studies suggest a correlation between the aging process and reducing enzymatic and non-enzymatic agents, with a consequent increasing level of ROS[18].

One of the most widely used natural antioxidants studied is ascorbic acid that eliminates most ROS due to the oxidation of ascorbate to monodehydroascorbate and then to dehydroascorbate and has other functions to maintain the normal physiologic state in humans. In the skin, ascorbic acid is a cofactor required for the enzymatic activity of prolyl hydroxylase, which hydroxylates prolyl resulting in procollagen and elastin[19].

Bogdan Allemann and Baumann revised the use of other antioxidants in skin care formulations and found that a 3-month daily regimen of topical using of ascorbic acid provided objective and subjective improvement in photodamaged facial skin[20].

It is common consensus that the cellular aging process can be prevented by plants’ phenolic substances, which has motivated the investigation of these plant metabolites and their possible action in the prevention of cellular aging[21].

The result shows the values of IC50of ethanol extract ofT. diversifoliadry flowers and ascorbic acid as a pattern, and points to a higher antioxidant activity of this plant extract when compared to the standard used ascorbic acid, showing the effectiveness of antioxidant activity.

Therefore, we suggest the possibility that flower extracts ofT. diversifoliacan control the action of free radical activities and thus preventing cellular aging, becoming an alternative in the fight against skin aging, since these plants are easy to grow and produce a lot of flowers during their flowering.

Finally, considering the results obtained, as future perspectives, we intend to evaluate some biological activities, such as wound-healing, anti-inflammatory, antimicrobial and anti-cancer activity, as well as quantify the main phytochemicals present in extracts.

Conflict of interest statement

We declare that we have no conflict of interest.

Acknowledgements

This work was supported by Pró-Saúde II, Funda??o do ABC (Grant No. 2261/2008).

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10.12980/APJTB.4.2014APJTB-2014-0055

*Corresponding author: José Armando-Junior, Laboratório de Pesquisa do Curso de Farmácia, Faculdade de Medicina do ABC, Avenida Príncipe de Gales, 821, Vila Príncipe de Gales, 09060-870, Santo André, SP, Brasil.

Tel: +55(11)4993-5404

E-mail: jose.junior@fmabc.br

Foundation Project: Supported by Pró-Saúde II, Funda??o do ABC (Grant No.

2261/2008).

Phytochemical screening

Tithonia diversifolia (Hemsl) A. Gray

Methods:The tests of phytochemical screening included total phenols, tannins, flavonoids, alkaloids and saponins. The active antioxidant was determined by 2,2-diphenyl-1-picrylhydrazyl method.

Results:The phytochemical screening of T. diversifolia dry flowers revealed the presence of phenolic compounds (tannins, flavonoids and total phenols), while alkaloids and saponins were not detected. The IC50values showed a strong antioxidant activity of the plant extracts.

Conclusions:Therefore, this study suggests the possibility of using dry flowers extracts of T. diversifolia for the prevention of cell aging, as was shown to have significant antioxidant activity.