• <tr id="yyy80"></tr>
  • <sup id="yyy80"></sup>
  • <tfoot id="yyy80"><noscript id="yyy80"></noscript></tfoot>
  • 99热精品在线国产_美女午夜性视频免费_国产精品国产高清国产av_av欧美777_自拍偷自拍亚洲精品老妇_亚洲熟女精品中文字幕_www日本黄色视频网_国产精品野战在线观看 ?

    Hepatitis C virus infection and biological falsepositive syphilis test: a single-center experience

    2011-07-05 12:59:45WeiFangZhuShuiYingLeiandLanJuanLi

    Wei-Fang Zhu, Shui-Ying Lei and Lan-Juan Li

    Hangzhou, China

    Hepatitis C virus infection and biological falsepositive syphilis test: a single-center experience

    Wei-Fang Zhu, Shui-Ying Lei and Lan-Juan Li

    Hangzhou, China

    BACKGROUND:Rapid plasma regain positive and/or treponema pallidum hemagglutination negative [RPR(+)/TPHA(-)] results were designated as biologic false-positive (BFP). There are limited data about BFP reactions against syphilis in patients with hepatitis C virus (HCV) infection. This study aimed to determine the prevalence of BFP reactions for syphilis in patients with HCV infection in a large sample and assess the relationship between BFP reactions and HCV infection.

    METHODS:A total of 2656 patients with positive anti-HCV and 5600 healthy control subjects were enrolled in this study. Hepatitis C serology was determined by a second generation ELISA test for HCV antibody. Syphilis serology was determined by the RPR test. Those subjects with reactive RPR positive underwent the TPHA test. Demographics and laboratory data were collected by trained clinicians.

    RESULTS:Among 2656 patients, 111 (4.2%) had a reactive RPR test. Of the 111 patients who were subjected to reactive RPR test, 30 (27.0%) showed HCV(+)/RPR(+). Of 5600 healthy controls, 80 (1.4%) had a reactive RPR test. Fourteen (17.5%) controls with HCV(-)/RPR(+) had a non-reactive TPHA test. These represented 1.1% of all HCV-positive and 0.3% of all HCV-negative subjects (P<0.001). A significantly increased prevalence shown by false-positive tests for syphilis was observed in elderly HCV-seropositive patients. BFP-HCV positive group had a higher prevalence of eosinophilia. The eosinophil abnormality was compared between the patients and controls (66.7% vs 21.4%,P=0.0043). No significant results were observed in antinuclear antibodies, antiphospholipid and complement (C3, C4) (P>0.05).

    CONCLUSIONS:The data of this study demonstrate that HCV infection is associated with a false-positive RPR test. In this study BFPs were significantly more common in HCV positive patients compared to HCV-negative ones. Eosinophil abnormality can be considered as a predictor for BFP. Excessive BFPs must be considered in assessing the frequency of syphilis in a HCV-positive population and the importance of the treponemal specific serologic test should be emphasized for a diagnosis of syphilis in such population.

    (Hepatobiliary Pancreat Dis Int 2011; 10: 399-402)

    hepatitis C virus; biological false-positive; syphilis

    Introduction

    The diagnosis of syphilis requires a two-step serological test. Not infrequently, sensitive screening is reactive, but it is not confirmed by a more specific test yielding a biological false-positive (BFP).[1]The rapid plasma reagin (RPR) test for syphilis screening may, under certain circumstances, yield positive results in patients not infected by Treponemapallidum. Occasional BFP may occur in any of the serological tests for syphilis. In general, BFP is more likely to be seen in autoimmune disease, HIV infection, pregnancy, and intravenous drug abuse.[2-5]The nonrecognition of BFP reaction for syphilis may have negative social and economic implications.

    An array of infectious and noninfectious conditions has been reported to cause BFP reaction.[2-5]Hepatitis C virus (HCV) infection has been associated with mixed cryoglobulinemia and immune complex glomerulonephriris,[6,7]but has rarely been investigated as a cause of BFP reactions in a large sample. This study aimed to describe the prevalence of BFP in a large HCV-infected or non-infected population.

    Methods

    A total of 2656 patients with HCV infection (patientsgroup) and 5600 healthy outpatients (control group) were enrolled in this study. The patients had been treated at the First Affiliated Hospital, Zhejiang University School of Medicine from December 1, 2008 to April 1, 2011.

    Healthy controls of comparable age and gender were recruited from our hospital. They had no history of cardiovascular, liver or kidney diseases. Clinical examination and laboratory test (liver and kidney function, hepatitis B surface antigen, HCV antibody and HIV antibody) were normal. The main exclusion criteria of both groups should included incomplete clinical data, current pregnancy, pre-existing hepatitis B, and HIV infection or connective tissue diseases.

    HCV antibody of all enrollees was determined by a second generation ELISA (Ortho Diagnostics) test. Syphilis detection was done using the RPR test; those with reactive RPR positive underwent the treponema pallidum hemagglutination (TPHA) test. Autoantibodies [antinuclear antibodies (ANA), antiphospholipid (aPL)] were detected in sera by indirect immunofluorescence. Eosinophil count was determined by an automated blood cells counter (Sysmex XS800i). The levels of complement (C3, C4) were measured by the photometry immuno-turbidimetric test (BECKMAN). The levels of γ-globulin were measured by cellulose acetate membrane electrophoresis (INTERLAB).

    Clinical and laboratory data were collected from records through an Excel spreadsheet (version 2003). Stratified analysis was made using the chi-square test or Fisher's exact test. A P value less than 0.05 was considered statistically significant.

    Results

    Of the 2656 HCV seropositive patients, 1100 were female and 1556 male, with an average age of 37 (range 20-69) years. Among the 5600 healthy subjects, 2101 were female and 3499 male, with an average age of 35 (range 19-71) years (Table 1). No significance was observed in gender and age between the two groups.

    One hundred and eleven (4.2%) patients with HCV seropositive and 80 (1.4%) controls with HCV seronegative were RPR reactive (P<0.0001). Thirty (27.0%) patients were HCV(+)/RPR(+) and 14 (17.5%) controls were HCV(-)/RPR(+). All of them had a nonreactive TPHA test and represented 1.1% of HCV-positive and 0.3% of HCV-negative subjects (P<0.001) (Fig. 1).

    Laboratory data were compared between the 30 BFP-HCV positive patients and 14 BFP-HCV negative controls (Table 2). False-positive tests for syphilis showed a significantly increased prevalence in elderly HCV seropositive patients (≥60 years old). The BFP-HCV positive group had a higher prevalence of eosinophilia. The eosinophil abnormality was compared between theBFP-HCV positive group and BFP-HCV negative group (66.7% vs 21.4%, P=0.0043). To investigate whether there is an association between BFP and immunological abnormality, we analyzed the results of ANA, aPL and complement (C3, C4). Seven (23.3%) HCV-positive BFP patients were ANA positive. Two (1.4%) patients with HCV-negative BFP were ANA positive. Four (13.3%) HCV-positive BFP patients were aPL positive. One (7.1%) of the HCV-negative BFP patients were aPL positive. Four (1.4%) of the HCV-negative BFP patients were aPL positive. But no significant results were observed in ANA, aPL and complement (C3, C4) (P>0.05, Fig. 2).

    Table 1. Demographic and laboratory characteristics of the patients and control groups

    Table 2. Demographic characteristics of the BFP group by HCV serostatus

    Fig. 1. BFP results as a proportion of reactive syphilis serological tests by HCV serostatus. *: A significantly increased prevalence in RPR reactive HCV between seropositive and controls with HCV seronegative. #: Significance was noted between HCV seropositive patients and HCV seronegative controls.

    Fig. 2. Laboratory findings of the BFP group by HCV serostatus. *: P<0.05, BFP-HCV positive group vs BFP-HCV negative group.

    We measured γ-globulin in 23 HCV-positive BFP patients and 10 HCV-negative BFP. Six (26.1%) of the HCV-positive BFP patients had a high level of serum γ-globulin. Two (20.0%) of the HCV-negative BFP patients had an abnormal level of serum γ-globulin.

    Discussion

    BFP serological test for syphilis has long been recognized. Reviewing the pertinent literature revealed that BFP may be associated with vaccinations, infections (infectious mononucleosis, measles, typhoid, varicella, influenza, lymphogranuloma venereum, malaria, etc), pregnancy, connective tissue diseases, multiple blood transfusions.[3,8-11]But the association of BFP reactions for syphilis with HCV has rarely been discussed. HCV infection associated with false-positive RPR test has been found mainly in patients with sexually transmitted disease.[12,13]In African-American women, Augenbraun et al[14]found that patients with HCV infection were 4-4.5 times more likely to have a false-positive reaction for syphilis than anti-HCV-negative patients. However, in this study we investigated HCV infection and BFP in Chinese including males and females in a local center. The HCV-positive group was 5 times more likely to have BFP for syphilis than the HCV-negative control group; this finding is similar to the results reported elsewhere.[12-14]

    BFP is known to be associated with autoimmune disease, HIV infection, pregnancy, and hepatitis B.[12,15-17]In our study, we excluded such populations in design. Thus we can explain why there was no difference in the prevalence of autobodies (ANA, aPL) and the level of complement (C3, C4) between the two groups. We observed that the BFP-HCV positive group had a higher prevalence of eosinophil abnormality (P=0.0043). The association with HCV and eosinophilia abnormality in the BFP group was not reported. This association has not been elucidated. We infer that eosinophilia may be associated with active hepatitis, but this needs further investigation. Studies[5,6,12]have confirmed the association of immunoglobulin production disorder with HCV infection. In this study, we also found that a higher prevalence of serum γ-globulin abnormality in the BFP-HCV positive patients compared with the BFPHCV negative patients.

    The proposed mechanism by which infectious pathogens cause BFP reactions includes coincidental cross-reactivity of an idiotype of the infecting pathogen with nontreponemal antigens and nonspectifically increased production of antibodies associated with infectious diseases.[16]HCV may be conclusively considered a cause of BFP. It may make an already nonspecific treponemal test less specific. HCV might alter the operational parameters of other treponemal-specific serological tests or serological tests used for other nonsyphilis clinical conditions. In our study, BFP may result from the cross-reactivity of HCV-induced antibodies with nontreponemal antigens in the RPR test.

    According to these data, RPR positive in hepatitis C may be BFP with regard to the reaginic tests. Therefore, therapeutic measures should not be initiated without confirmation with a TPHA test.

    This study has drawbacks. We have small positive BFP samples because of the lower prevalence rate of BFP in China. Traditionally BFP reactions were classified as acute and chronic ones. We couldn’t get the result because we failed to follow up further.

    In conclusion, the data of this study demonstrate that HCV infection is associated with false-positive RPR test results. BFP was more common in HCV-positive patients than in HCV-negative patients. Eosinophil abnormality can be considered as a predictor for BFP. Excessive BFP must be considered in assessing the frequency of syphilis in HCV-positive population as well as the importance of the specific serologic TPHA test in confirming a diagnosis of syphilis in such population.

    Acknowledgement

    We thank to Dr. Da-Gan Yang and Shu-Juan Song for their technical assistance.

    Funding:None.

    Ethical approval:Not needed.

    Contributors:ZWF and LSY contributed equally to this study. ZWF proposed the study. ZWF and LSY wrote the first draft. All authors contributed to the design and interpretation of the study and to further drafts. LLJ is the guarantor.

    Competing interest:No benefits in any form have been received or will be received from a commercial party related directly or indirectly to the subject of this article.

    1 Nandwani R, Evans DT. Are you sure it's syphilis? A review of false positive serology. Int J STD AIDS 1995;6:241-248.

    2 Geusau A, Kittler H, Hein U, Dangl-Erlach E, Stingl G, Tschachler E. Biological false-positive tests comprise a high proportion of Venereal Disease Research Laboratory reactions in an analysis of 300,000 sera. Int J STD AIDS 2005; 16:722-726.

    3 Frederick T, Burian P, Terrault N, Cohen M, Augenbraun M, Young M, et al. Factors associated with prevalent hepatitis C infection among HIV-infected women with no reported history of injection drug use: the Women's Interagency HIV Study (WIHS). AIDS Patient Care STDS 2009;23:915-923.

    4 Rompalo AM, Cannon RO, Quinn TC, Hook EW 3rd. Association of biologic false-positive reactions for syphilis with human immunodeficiency virus infection. J Infect Dis 1992;165:1124-1126.

    5 Koike T, Sueishi M, Funaki H, Tomioka H, Yoshida S. Antiphospholipid antibodies and biological false positive serological test for syphilis in patients with systemic lupus erythematosus. Clin Exp Immunol 1984;56:193-199.

    6 Operskalski EA, Mack WJ, Strickler HD, French AL, Augenbraun M, Tien PC, et al. Factors associated with hepatitis C viremia in a large cohort of HIV-infected anduninfected women. J Clin Virol 2008;41:255-263.

    7 Li YD, Lin JJ, Zheng SS. Inflammatory bowel diseases and hepatitis C virus infection. Hepatobiliary Pancreat Dis Int 2010;9:398-401.

    8 Augenbraun MH, DeHovitz JA, Feldman J, Clarke L, Landesman S, Minkoff HM. Biological false-positive syphilis test results for women infected with human immunodeficiency virus. Clin Infect Dis 1994;19:1040-1044.

    9 Barkan SE, Melnick SL, Preston-Martin S, Weber K, Kalish LA, Miotti P, et al. The Women's Interagency HIV Study. WIHS Collaborative Study Group. Epidemiology 1998;9:117-125.

    10 Hernández-Aguado I, Bolumar F, Moreno R, Pardo FJ, Torres N, Belda J, et al. False-positive tests for syphilis associated with human immunodeficiency virus and hepatitis B virus infection among intravenous drug abusers. Valencian Study Group on HIV Epidemiology. Eur J Clin Microbiol Infect Dis 1998;17:784-787.

    11 Cabrini JM, Bottasso OA, Margasin S, Schujman L, Mangiaterra L, Morini JC. Biological false positive test for syphilis in lepromatous leprosy patients with concomitant hepatitis B virus infection. J Investig Allergol Clin Immunol 1991;1:45-48.

    12 Thomas DL, Rompalo AM, Zenilman J, Hoover D, Hook EW 3rd, Quinn TC. Association of hepatitis C virus infection with false-positive tests for syphilis. J Infect Dis 1994;170:1579-1581.

    13 Sonmez E, Ozerol IH, Senol M, Kizilkaya N, Sahin K, Ozbilge H. False-positive reaction between syphilis and hepatitis C infection. Isr J Med Sci 1997;33:724-727.

    14 Augenbraun M, French A, Glesby M, Sanchez-Keeland L, Young M, Greenblatt R, et al. Hepatitis C virus infection and biological false-positive syphilis tests. Sex Transm Infect 2010;86:97-98.

    15 Huh HJ, Lee KK, Kim ES, Chae SL. Analysis of positive results in mediace rapid plasma reagin and Treponema pallidum latex agglutination as the automated syphilis test. Korean J Lab Med 2007;27:324-329.

    16 Erbelding EJ, Vlahov D, Nelson KE, Rompalo AM, Cohn S, Sanchez P, et al. Syphilis serology in human immunodeficiency virus infection: evidence for false-negative fluorescent treponemal testing. J Infect Dis 1997;176:1397-1400.

    17 Conley CL, Savarese DM. Biologic false-positive serologic tests for syphilis and other serologic abnormalities in autoimmune hemolytic anemia and thrombocytopenic purpura. Medicine (Baltimore) 1989;68:67-84.

    Received May 21, 2011

    Accepted after revision July 13, 2011

    Author Affiliations: State Key Laboratory for Diagnosis and Treatment of Infectious Diseases; Key Laboratory of Infectious Diseases, Zhejiang Province; Department of Infectious Diseases, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China (Zhu WF, Lei SY and Li LJ)

    Lan-Juan Li, MD, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases; Key Laboratory of Infectious Diseases, Zhejiang Province; Department of Infectious Diseases, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China (Tel: 86-571-87236340; Email: ljli@zju.edu.cn)

    ? 2011, Hepatobiliary Pancreat Dis Int. All rights reserved.

    色在线成人网| 亚洲欧美日韩无卡精品| 久久天堂一区二区三区四区| 男女下面插进去视频免费观看| 亚洲人成电影免费在线| 免费在线观看日本一区| 久久久久久人人人人人| 黄色视频不卡| 国产欧美日韩综合在线一区二区| 人人妻人人添人人爽欧美一区卜| 亚洲精品一卡2卡三卡4卡5卡| 午夜影院日韩av| 亚洲av日韩精品久久久久久密| 国产精品成人在线| 夜夜躁狠狠躁天天躁| 国产精品一区二区精品视频观看| 他把我摸到了高潮在线观看| 麻豆av在线久日| 高清av免费在线| 又黄又粗又硬又大视频| a级毛片在线看网站| 首页视频小说图片口味搜索| 久久久久久久久中文| 亚洲精品中文字幕在线视频| 成年人免费黄色播放视频| 亚洲人成电影观看| 黄色a级毛片大全视频| 国产视频一区二区在线看| 久久人人精品亚洲av| 亚洲av日韩精品久久久久久密| 国产熟女午夜一区二区三区| 久久青草综合色| 亚洲成人免费电影在线观看| 91精品三级在线观看| 大香蕉久久成人网| 国产精品一区二区在线不卡| 精品国产超薄肉色丝袜足j| 国产高清激情床上av| 国产有黄有色有爽视频| 欧美色视频一区免费| 桃色一区二区三区在线观看| 中文字幕av电影在线播放| 亚洲中文日韩欧美视频| 涩涩av久久男人的天堂| 美女高潮喷水抽搐中文字幕| 久久午夜亚洲精品久久| 国产1区2区3区精品| 成人av一区二区三区在线看| 国产精品爽爽va在线观看网站 | 自线自在国产av| 天堂动漫精品| 一级作爱视频免费观看| 咕卡用的链子| 国产又爽黄色视频| 18禁裸乳无遮挡免费网站照片 | www.www免费av| 天堂影院成人在线观看| 12—13女人毛片做爰片一| 亚洲熟妇中文字幕五十中出 | 乱人伦中国视频| 成人亚洲精品一区在线观看| 三级毛片av免费| 亚洲av成人一区二区三| 在线视频色国产色| 中亚洲国语对白在线视频| √禁漫天堂资源中文www| 亚洲色图av天堂| 久久精品国产亚洲av香蕉五月| 老鸭窝网址在线观看| 亚洲avbb在线观看| 亚洲欧美精品综合一区二区三区| 亚洲成av片中文字幕在线观看| 欧美日韩视频精品一区| 精品国产国语对白av| 涩涩av久久男人的天堂| a级毛片在线看网站| 亚洲精品国产色婷婷电影| 亚洲精品成人av观看孕妇| 国产av一区二区精品久久| 久久99一区二区三区| 熟女少妇亚洲综合色aaa.| 手机成人av网站| 久热这里只有精品99| 最近最新免费中文字幕在线| 一级毛片女人18水好多| 午夜影院日韩av| 亚洲精品中文字幕一二三四区| 高清av免费在线| 亚洲专区国产一区二区| 在线天堂中文资源库| 操出白浆在线播放| 中文字幕精品免费在线观看视频| 国产蜜桃级精品一区二区三区| 久久精品成人免费网站| 国产激情欧美一区二区| 国产精品久久久av美女十八| 免费搜索国产男女视频| 正在播放国产对白刺激| 国产精品综合久久久久久久免费 | 亚洲aⅴ乱码一区二区在线播放 | 免费女性裸体啪啪无遮挡网站| 亚洲成国产人片在线观看| 757午夜福利合集在线观看| 久久亚洲精品不卡| 中文字幕人妻熟女乱码| 两性午夜刺激爽爽歪歪视频在线观看 | 国产精品九九99| 99精品久久久久人妻精品| 久久香蕉精品热| 午夜福利一区二区在线看| 高清毛片免费观看视频网站 | 黄色女人牲交| 男人操女人黄网站| 亚洲av成人一区二区三| 男女做爰动态图高潮gif福利片 | 老司机午夜十八禁免费视频| 欧美 亚洲 国产 日韩一| 国产xxxxx性猛交| 国产精品久久视频播放| 丁香六月欧美| 美女午夜性视频免费| 久久天躁狠狠躁夜夜2o2o| 黑人欧美特级aaaaaa片| 国产av在哪里看| 欧美成人性av电影在线观看| 大码成人一级视频| 国产亚洲av高清不卡| 国产精品二区激情视频| 首页视频小说图片口味搜索| 成人特级黄色片久久久久久久| 亚洲熟妇熟女久久| 中文字幕色久视频| 在线观看www视频免费| 亚洲熟妇熟女久久| 成人亚洲精品av一区二区 | 欧美日韩福利视频一区二区| 在线十欧美十亚洲十日本专区| 国产主播在线观看一区二区| av福利片在线| 又黄又爽又免费观看的视频| 亚洲熟妇中文字幕五十中出 | 一区二区日韩欧美中文字幕| 一级作爱视频免费观看| 手机成人av网站| 88av欧美| 色婷婷av一区二区三区视频| 黑人巨大精品欧美一区二区mp4| 91精品三级在线观看| 性少妇av在线| 亚洲一区中文字幕在线| 美女高潮到喷水免费观看| 久久久国产成人精品二区 | 操出白浆在线播放| av免费在线观看网站| 午夜福利,免费看| 日本一区二区免费在线视频| 老司机在亚洲福利影院| 午夜免费观看网址| 亚洲一区高清亚洲精品| 身体一侧抽搐| 日韩视频一区二区在线观看| 国产精品偷伦视频观看了| 国产精品偷伦视频观看了| 美女高潮喷水抽搐中文字幕| 亚洲 欧美一区二区三区| 在线免费观看的www视频| 国产精品久久电影中文字幕| 电影成人av| avwww免费| 一区二区三区激情视频| 99精品在免费线老司机午夜| 成人亚洲精品av一区二区 | 18美女黄网站色大片免费观看| 人人妻人人爽人人添夜夜欢视频| 免费看a级黄色片| 激情视频va一区二区三区| 高清欧美精品videossex| 50天的宝宝边吃奶边哭怎么回事| 成人影院久久| 久久这里只有精品19| 一边摸一边抽搐一进一小说| 日本 av在线| 12—13女人毛片做爰片一| 欧美日韩国产mv在线观看视频| www国产在线视频色| 一边摸一边抽搐一进一小说| 久久久久久久午夜电影 | 别揉我奶头~嗯~啊~动态视频| 欧美日韩亚洲综合一区二区三区_| 男女做爰动态图高潮gif福利片 | 午夜老司机福利片| 午夜免费激情av| 亚洲七黄色美女视频| www.999成人在线观看| 亚洲欧美日韩无卡精品| 热re99久久精品国产66热6| 久久 成人 亚洲| 老司机午夜福利在线观看视频| 午夜免费激情av| 久久人妻熟女aⅴ| 国产91精品成人一区二区三区| 国产精品影院久久| 国产精品国产高清国产av| 久久人人爽av亚洲精品天堂| 日韩一卡2卡3卡4卡2021年| 国产伦人伦偷精品视频| av有码第一页| 91麻豆精品激情在线观看国产 | 一边摸一边抽搐一进一小说| 欧美激情极品国产一区二区三区| 美女午夜性视频免费| 亚洲伊人色综图| 色哟哟哟哟哟哟| 曰老女人黄片| 脱女人内裤的视频| 成年女人毛片免费观看观看9| 最近最新免费中文字幕在线| 国产有黄有色有爽视频| 亚洲精品粉嫩美女一区| 91麻豆精品激情在线观看国产 | 免费搜索国产男女视频| 国产精品九九99| 久久亚洲精品不卡| 欧美av亚洲av综合av国产av| 乱人伦中国视频| 免费看a级黄色片| 热re99久久精品国产66热6| 色老头精品视频在线观看| 99国产精品99久久久久| 精品久久久精品久久久| 真人做人爱边吃奶动态| 淫妇啪啪啪对白视频| 欧美中文综合在线视频| 精品午夜福利视频在线观看一区| 男人操女人黄网站| 久9热在线精品视频| 日韩有码中文字幕| 黄频高清免费视频| 一级黄色大片毛片| 亚洲avbb在线观看| 国产欧美日韩一区二区三| 黄色毛片三级朝国网站| 久久久久精品国产欧美久久久| 性欧美人与动物交配| 人人妻人人澡人人看| 亚洲人成电影观看| 波多野结衣一区麻豆| 在线观看午夜福利视频| 黄色视频,在线免费观看| 两个人免费观看高清视频| 国产亚洲精品第一综合不卡| 村上凉子中文字幕在线| 国产xxxxx性猛交| 国产av在哪里看| 亚洲成人久久性| 亚洲自拍偷在线| 亚洲,欧美精品.| 99国产综合亚洲精品| av网站免费在线观看视频| 人人妻人人澡人人看| 欧洲精品卡2卡3卡4卡5卡区| 久久人人爽av亚洲精品天堂| 国产伦一二天堂av在线观看| 国产精品98久久久久久宅男小说| 成人国产一区最新在线观看| 日本欧美视频一区| 99国产精品免费福利视频| 亚洲性夜色夜夜综合| 最近最新免费中文字幕在线| 69精品国产乱码久久久| 精品久久久久久成人av| 中文字幕av电影在线播放| 国产亚洲精品一区二区www| 亚洲欧美激情在线| 国产男靠女视频免费网站| 婷婷六月久久综合丁香| 成人免费观看视频高清| 亚洲精品中文字幕一二三四区| 国产黄色免费在线视频| 黄色片一级片一级黄色片| 男女高潮啪啪啪动态图| 亚洲 欧美一区二区三区| 老司机深夜福利视频在线观看| 亚洲精品在线美女| 亚洲欧美日韩无卡精品| 天堂动漫精品| 夜夜爽天天搞| 桃色一区二区三区在线观看| 黄片播放在线免费| 中文字幕精品免费在线观看视频| 亚洲国产欧美日韩在线播放| 美女午夜性视频免费| 日本黄色日本黄色录像| 亚洲精品中文字幕一二三四区| 一级作爱视频免费观看| 欧美中文综合在线视频| 亚洲国产欧美网| 成人特级黄色片久久久久久久| 亚洲成人免费av在线播放| a级片在线免费高清观看视频| 天堂中文最新版在线下载| 午夜精品在线福利| 亚洲一卡2卡3卡4卡5卡精品中文| 免费av中文字幕在线| 狂野欧美激情性xxxx| 欧美另类亚洲清纯唯美| 国产激情欧美一区二区| 麻豆成人av在线观看| 日韩成人在线观看一区二区三区| 久久中文字幕一级| 精品日产1卡2卡| 女同久久另类99精品国产91| 久久中文看片网| 亚洲人成电影免费在线| 亚洲自偷自拍图片 自拍| 亚洲国产看品久久| 狠狠狠狠99中文字幕| 欧洲精品卡2卡3卡4卡5卡区| 国产一区二区三区视频了| 久久久久久大精品| av片东京热男人的天堂| 欧美日韩瑟瑟在线播放| 日韩欧美三级三区| 国产精品秋霞免费鲁丝片| 又黄又粗又硬又大视频| 国产一卡二卡三卡精品| 精品熟女少妇八av免费久了| 久久精品国产综合久久久| 国产精品香港三级国产av潘金莲| 18美女黄网站色大片免费观看| 热re99久久国产66热| 日韩欧美一区视频在线观看| 久久九九热精品免费| 一级毛片高清免费大全| 国产主播在线观看一区二区| 真人一进一出gif抽搐免费| 国产1区2区3区精品| 巨乳人妻的诱惑在线观看| 中文欧美无线码| 亚洲一区二区三区不卡视频| 国产欧美日韩一区二区三| 国产欧美日韩综合在线一区二区| av电影中文网址| 亚洲视频免费观看视频| 亚洲一卡2卡3卡4卡5卡精品中文| 丝袜美足系列| 国产xxxxx性猛交| 久久久久九九精品影院| 丝袜美足系列| 80岁老熟妇乱子伦牲交| 国产欧美日韩一区二区精品| 看黄色毛片网站| 亚洲片人在线观看| 久久午夜亚洲精品久久| 欧美人与性动交α欧美软件| 国产91精品成人一区二区三区| 日韩欧美免费精品| 80岁老熟妇乱子伦牲交| 中亚洲国语对白在线视频| 国产99久久九九免费精品| 国产99白浆流出| 18禁美女被吸乳视频| av天堂久久9| 高清欧美精品videossex| 99在线人妻在线中文字幕| x7x7x7水蜜桃| 成人特级黄色片久久久久久久| 性色av乱码一区二区三区2| 欧美久久黑人一区二区| 精品久久久久久,| 成年人黄色毛片网站| 两性午夜刺激爽爽歪歪视频在线观看 | 一边摸一边做爽爽视频免费| 午夜福利在线免费观看网站| 久久影院123| 日韩 欧美 亚洲 中文字幕| 三级毛片av免费| 亚洲人成网站在线播放欧美日韩| 国产精品自产拍在线观看55亚洲| 在线播放国产精品三级| 女人高潮潮喷娇喘18禁视频| 如日韩欧美国产精品一区二区三区| 久久精品亚洲熟妇少妇任你| 色综合站精品国产| 搡老乐熟女国产| 亚洲国产看品久久| e午夜精品久久久久久久| 欧美日韩精品网址| 亚洲成av片中文字幕在线观看| 成人三级做爰电影| 亚洲成人久久性| 后天国语完整版免费观看| 999久久久精品免费观看国产| 欧美成狂野欧美在线观看| 欧美亚洲日本最大视频资源| 久久久精品国产亚洲av高清涩受| 欧美日本亚洲视频在线播放| 欧美日韩亚洲国产一区二区在线观看| 80岁老熟妇乱子伦牲交| 黄片播放在线免费| 国产亚洲欧美在线一区二区| 国产亚洲精品一区二区www| 一夜夜www| a级毛片黄视频| 麻豆av在线久日| 久久精品91蜜桃| 久久久国产成人免费| 久久人人97超碰香蕉20202| 免费在线观看亚洲国产| 国产真人三级小视频在线观看| 美女扒开内裤让男人捅视频| 欧美老熟妇乱子伦牲交| 日日摸夜夜添夜夜添小说| 露出奶头的视频| 国产伦一二天堂av在线观看| 母亲3免费完整高清在线观看| 黄色成人免费大全| tocl精华| 日韩精品青青久久久久久| 十分钟在线观看高清视频www| 国产xxxxx性猛交| 国内久久婷婷六月综合欲色啪| 99热只有精品国产| 热re99久久国产66热| 精品国产乱码久久久久久男人| 欧美激情久久久久久爽电影 | 男女高潮啪啪啪动态图| 十八禁网站免费在线| 成人精品一区二区免费| 久久精品国产亚洲av高清一级| 国产精品98久久久久久宅男小说| 午夜精品在线福利| 精品人妻1区二区| 婷婷丁香在线五月| 久热这里只有精品99| 国产高清国产精品国产三级| 亚洲久久久国产精品| 久久 成人 亚洲| 国产有黄有色有爽视频| 国产精品98久久久久久宅男小说| 国产欧美日韩一区二区精品| 免费看a级黄色片| 欧美日韩亚洲国产一区二区在线观看| 午夜福利,免费看| 国产1区2区3区精品| 免费观看人在逋| 国产精品一区二区精品视频观看| 波多野结衣一区麻豆| 人人妻,人人澡人人爽秒播| 精品午夜福利视频在线观看一区| 老司机靠b影院| 69精品国产乱码久久久| 久久人人精品亚洲av| 在线av久久热| 久热这里只有精品99| 高潮久久久久久久久久久不卡| 一个人免费在线观看的高清视频| 欧美精品啪啪一区二区三区| 国产精品偷伦视频观看了| www.自偷自拍.com| 免费久久久久久久精品成人欧美视频| 欧美黑人欧美精品刺激| 色综合婷婷激情| 99国产精品一区二区蜜桃av| 身体一侧抽搐| 性欧美人与动物交配| 另类亚洲欧美激情| 麻豆一二三区av精品| 51午夜福利影视在线观看| 黑人巨大精品欧美一区二区mp4| av福利片在线| 天天影视国产精品| 黑人欧美特级aaaaaa片| 黄色视频,在线免费观看| 精品福利观看| av福利片在线| 精品国产一区二区三区四区第35| 国产在线精品亚洲第一网站| 精品久久久久久久毛片微露脸| 色婷婷久久久亚洲欧美| 午夜精品在线福利| 波多野结衣av一区二区av| 男女高潮啪啪啪动态图| 国产午夜精品久久久久久| 日日爽夜夜爽网站| 久久精品国产亚洲av高清一级| 国产亚洲精品久久久久久毛片| 视频区图区小说| 亚洲国产看品久久| 久久青草综合色| 国产伦人伦偷精品视频| 日韩中文字幕欧美一区二区| 99久久精品国产亚洲精品| 久久精品国产亚洲av香蕉五月| 国产精品电影一区二区三区| 亚洲七黄色美女视频| 在线观看www视频免费| 99久久精品国产亚洲精品| 午夜福利一区二区在线看| 亚洲精品av麻豆狂野| 18禁美女被吸乳视频| 成人特级黄色片久久久久久久| 免费在线观看亚洲国产| 精品国产美女av久久久久小说| 在线观看免费高清a一片| 日日干狠狠操夜夜爽| 不卡一级毛片| www国产在线视频色| 99热只有精品国产| 国产成+人综合+亚洲专区| 高清毛片免费观看视频网站 | 麻豆国产av国片精品| 亚洲国产精品999在线| 黑人欧美特级aaaaaa片| 中文字幕另类日韩欧美亚洲嫩草| 国产亚洲精品久久久久久毛片| 很黄的视频免费| 80岁老熟妇乱子伦牲交| 在线国产一区二区在线| 色精品久久人妻99蜜桃| 亚洲全国av大片| 午夜久久久在线观看| 国产精品九九99| 欧美日韩av久久| 免费在线观看黄色视频的| 久久狼人影院| 免费一级毛片在线播放高清视频 | 免费av中文字幕在线| 琪琪午夜伦伦电影理论片6080| 亚洲一区中文字幕在线| 精品熟女少妇八av免费久了| 在线观看免费视频网站a站| 亚洲色图综合在线观看| 亚洲aⅴ乱码一区二区在线播放 | www.精华液| av有码第一页| 九色亚洲精品在线播放| 成人黄色视频免费在线看| 国产成人系列免费观看| 脱女人内裤的视频| 交换朋友夫妻互换小说| 自线自在国产av| 亚洲精品国产色婷婷电影| 天天添夜夜摸| 日本黄色视频三级网站网址| 成年女人毛片免费观看观看9| 日本 av在线| 最近最新中文字幕大全电影3 | 午夜精品在线福利| 妹子高潮喷水视频| 在线观看免费午夜福利视频| 精品电影一区二区在线| 97超级碰碰碰精品色视频在线观看| 亚洲成人免费av在线播放| 久久久久国产一级毛片高清牌| 久久国产精品男人的天堂亚洲| 国产成人啪精品午夜网站| 免费看十八禁软件| 精品国产亚洲在线| 真人一进一出gif抽搐免费| 色哟哟哟哟哟哟| 午夜老司机福利片| 亚洲国产精品999在线| 夫妻午夜视频| 宅男免费午夜| 免费一级毛片在线播放高清视频 | 一级片免费观看大全| 国产无遮挡羞羞视频在线观看| 久99久视频精品免费| 午夜影院日韩av| www.精华液| 18禁国产床啪视频网站| 怎么达到女性高潮| 国产一区二区三区在线臀色熟女 | 69av精品久久久久久| 国产亚洲av高清不卡| 男人操女人黄网站| 午夜免费成人在线视频| 亚洲第一欧美日韩一区二区三区| 午夜a级毛片| 最新美女视频免费是黄的| 啦啦啦 在线观看视频| 欧美乱码精品一区二区三区| 亚洲国产精品一区二区三区在线| 亚洲精品成人av观看孕妇| 午夜免费激情av| 精品电影一区二区在线| 久久久国产成人精品二区 | www.精华液| 精品乱码久久久久久99久播| 精品久久久久久久久久免费视频 | 久久这里只有精品19| 亚洲第一av免费看| 黄色丝袜av网址大全| 啦啦啦 在线观看视频| 一本综合久久免费| 亚洲一区二区三区欧美精品| 身体一侧抽搐| 99热国产这里只有精品6| 久久人人精品亚洲av| 久久久久精品国产欧美久久久| 国产精品九九99| 亚洲午夜精品一区,二区,三区| 69精品国产乱码久久久| 免费av毛片视频| 不卡av一区二区三区| 黄色片一级片一级黄色片| 久久天堂一区二区三区四区| 欧美国产精品va在线观看不卡| 免费在线观看黄色视频的| 亚洲第一青青草原| 激情在线观看视频在线高清| 三级毛片av免费| 亚洲成av片中文字幕在线观看| 亚洲午夜精品一区,二区,三区|