中國肉盤衣屬地衣新記錄種

朱孟麗, 趙欣, 王偉成, 賈澤峰*

中國肉盤衣屬地衣新記錄種

朱孟麗1*, 趙欣1*, 王偉成2,3, 賈澤峰1**

(1.聊城大學生命科學學院，山東 聊城 252059；2.中國科學院微生物研究所，北京 100101；3.中國科學院大學，北京 100049)

在對海南省葉生地衣資源的調(diào)查研究中發(fā)現(xiàn)了中國肉盤衣屬地衣新記錄種：乳頭肉盤衣[(Vain.) Lücking & Sérus.]，描述了其形態(tài)學、解剖學及化學特征，提供了顯微結構照片。主要識別特征為：地衣體殼狀，布滿灰綠色乳頭狀顆粒，子囊盤棕色，囊盤被外部由疏絲組織構成，子囊孢子(3～)7隔，26～36m×2～3m，呈末端變窄的細棍棒狀。基于線粒體小亞基(mtSSU)序列構建的系統(tǒng)發(fā)育樹表明該種與本屬模式種(Müll.Arg.) Vězda具有較近的親緣關系。編制了中國該屬地衣的分種檢索表。

乳頭肉盤衣；茶漬綱；地衣型真菌；mtSSU；分類學；新記錄

The genus, belonging to family Pilocarpaceae, in order, class Lecanoro- mycetes was established in 1986 with its type(Müll.Arg.) Vězda[1-2], including more than 90 species worldwide[3-4].is the second largest genus among foliicolous lichens next toAcharius[5]and quite diverse in morphological fea- tures[6].Thedistinctive traits of this genus are the comparatively small apothecia with a thin margin; paraplectenchymatous excipulum; usually indistinct, branched, and sparsely to densely anastomosing para- physes;-type asci; ellipsoid to cylindrical, transversely septate to muriform ascospores; and pycnidial conidiomata[6].are mostly folii- colous, common in pan-tropical ecologies.

China had previously reported ninespecies, which are distributed in Yunnan, Hainan, Hongkong and Taiwan[7-13].Based on specimens collected from Hainan Province,(Vain.) Lücking & Sérus.is found new to China.

1 Materials and methods

1.1 Specimens and morphology

The specimens of new record are deposited in the Fungarium of College of Life Sciences, Liaocheng University (LCUF) and Herbarium Mycologicum Aca- demiae Sinicae-Lichenes (HMAS-L).A dissecting microscope (Olympus SZX16) and a light microscope (Olympus BX53) were used for the morphological and anatomical studies.Measurements were taken from mature vertical sections of fruit bodies mounted in water.

1.2 Chemistry

Amyloidity of the ascospores was tested using Lugol’s solution.Spot tests with K (10% aqueous solution of potassium hydroxide), C (saturated solution of aqueous sodium hypochlorite), and P (saturated solution of-phenylenediamine in 95% ethyl alcohol) were performed on the thallus surface.The lichen substances were detected and identified by thin-layer chromatography, using solvent C[14-16].

1.3 DNA extraction, amplification, and sequencing

Genomic DNA was extractedfrom ascomata of the specimens using the Hi-DNAsecure Plant Kit (Tiangen, Beijing, China) according tothe manufac- turer’s protocol.PCR amplification was performed using the mtSSU1 and mtSSU3R primer pair for mtSSU[17].The 25L PCR reaction system containing 1L each primer solution (10mol/L), 0.5Lgenomic DNA, 10L ddH2O, and 12.5L2×PCR Master- Mix (Tiangen, Beijing, China).Thermocyclingcondi- tions comprised initial denaturation at 95℃(5 min); 35 denaturation cycles at 94 ℃(45 s), annealing at 50 ℃(1 min), extension at 72 ℃(1.5 min) and a final extension at 72 ℃(10 min).The target product of PCR was affirmed by electrophoresis on 1% agarose gels and sequenced by Biosune Inc.(Shanghai).Nine newly generated sequences were submitted to GenBank.The sequences obtained were evaluated using BLASTn and combined with selected sequences of Pilocarpaceae from GenBank (Fig.1),andwere used as the outgroup based on Ekman et al.[18]

1.4 Phylogenetic analysis

Contigs were assembled and edited using the program Geneious v.6.1.2 (Biomatters Ltd., Auckland, NZ).A total of 39 sequences were aligned using MAFFT v.7[19].The program Gblocks v.0.91b was used to delimit ambiguous regions, implementing all the options for a less stringent selection (http://molevol.cmima.csic.es/castresana/Gblocks_server.html)[20], which yielded final alignment of 670 bp.Maximum likely- hood (ML) and Bayesian inference (BI) were per- formed using the CIPRES Scientific gateway portal (http://www.phylo.org/portal2/)[21].Maximum likelihood bootstrapping analysis was performed with RAxML- HPC v.8[22], using the default parameters as imple- mented on the CIPRES, NSF XSEDE resource with bootstrap statistics calculated from 1 000 bootstrap replicates.For the Bayesian analysis, the best substi- tution model was estimated using jModelTest 2.1.6[23].Based on the results, we used GTR+I+G model.Baye- sian analysis was performed using MrBayes v.3.2.2 on CIPRES with 2 independent runs, searching for 10 000 000 generations with four independent chains and sampling every 1000th tree[24].After discarding the burn-in, the remaining 7 500 trees of each run werepooled to calculate a 50% majority rule consensus tree.Generated phylogenetic tree was visualized under Figtree v.1.4.2[25].

2 Results

2.1 Phylogenetic analysis

The final alignment consisted of 9 newly generated mtSSU sequences and 30 sequences downloaded from NCBI (Fig.1).The phylogenetic trees obtained from maximum likelihood (ML) and Bayesian inference analysis (BI) exhibited the same topology; we there- fore present only the ML tree.The molecular phylo- geny based on the mitochondrial small subunit marker (mtSSU) of Pilocarpaceae exhibits a well-supported monophyletic lineage containing the genera,,,,,and.The tree showsis polyphyletic in its current delimitation.is revealed as a sister clade to the type speciesThese two species together with,andform a monophyletic lineage without good support (BS=59%, PP=0.85).，,,andcluster together and get a high support (BS=96%, PP=1.0).While anotherspecies,shows a close relationship with.

2.2 Taxonomy

(Vain.) Lücking & Sérus, in Lichenologist 33(3): 192 (2001) Fig.2

Fig.1 Maximum likelihood tree of Fellhanera mastothallina and related species within Pilocarpaceae based on the mitochondrial small subunit marker (mtSSU).ML bootstrap values and MCMC posterior probabilities (second value) are displayed above each branch.Branches recovered with ML-BS support ≥70% and BI-PP support ≥0.95 were regarded as strongly supported.GenBank accessions are attached to the sequences.Newly generated sequences are shown in bold.

≡Vain., in Ann.Acad.Sci.Fenn., ser.A, 15: 64 (1921)

≡(Vain.) Vězda, in Vězda et al., Ann.Naturh.Mus.Wien 99B: 738 (1997)

Type: Philippines, Robinson & Ramos 11900 (TUR-holotype).

Description:Thallus foliicolous, crustose, conti- nuous, verrucose, 10-40 mm across and 10-15m thick, greyish-green, rough, often irregular in outline, with light green to orange-red verrucosa of 0.01-0.03 mm diam.Apothecia when mature sessile, rounded, 0.2-0.5 mm diam.and 150-200m high; disc plane to slightly convex, brown to dark brown; margin distinct and thin, about 0.1 mm wide, usually accom- panied by a white pruina.Excipulum light grey to light brown, 7-15m thick, internal parts appear paraplectenchymatuous and external parts prosoplec- tenchymatous.Hymenium 40-66m high, colourless.Hypothecium 26-74m high, brown to dark brown.Asci clavate, 36-48m×4-9m.Ascospores 8 per ascus, narrowly clavate, and tapering towards pro- ximal end, (3-)7-septate, 26-36m×2-3m, about 10-13 times as long as broad; I+ violet.Pycnidia not observed.

Chemistry: Trace amounts of substances were detected by TLC.Spot tests on thallus: K+ dark brown, C-, P-, KC+ dark brown.

Ecology and distribution: The species is a typi- cally foliicolous lichen, grows mainly in tropical Asia and also reported in Papua New Guinea, Australia and New Caledonia[26].New to China.

Specimen examined: CHINA.Hainan: WuzhishanCity, Wuzhishan Nature Reserve, on leaves, 18°54′27″ N, 109°40′48″ E, elev.730 m, 12 Dec.2019,Y.H.JuHN19446-a(GB accession No.: MW045559),HN19458(GB accession No.: MW045560),HN19459 (LCUF).Changjiang County, Bawangling Nature Reserve, Bai- shitan Scenic Area, on leaves, 19°7′17″ N, 109°4′53″E, elev.700 m, 4 Sep, 2017,W.C.Wang HN20170025 (HMAS-L 139457, GB accession No.: MW553282); Yajia Scenic Area, on leaves, 19o7′17″N, 109o4′53″, elev.550 m, 5 Sep.2017.W.C.WangHN20170124(HMAS-L 139601, GB accession No.: MW553281).

Fig.2 Fellhanera mastothallina (Y.H.Ju HN19458).A: Thallus with apothecia; B: Vertical section of an apothecium; C: Part of a vertical section of an apothecium; D: Ascospores.Bars: A=0.5 mm, B=100 μm, C-D=20 μm

3 Discussion

Phylogeneticallyis close related to the type speciesthis result confirms the hypothesis thatbelonged togroup by Lücking based on evident from thallus and apothecial characters[6].can be distinguished by reddish brown apothecia, paraplectenchymatous exciple and shorter ascospores (18-24m×3-4.5m).The mor- phological characteristics of our specimens collected from Hainan are almost identical with the type speci- men from Phillippines and materials from Papua New Guinea and New Caledonia except for the longer and narrower ascospores (the latter are 22-32m×3- 4m)[26-27].Our phylogenetic result thatbeing heterogeneous in its current circumscription is coincident with the preliminary studies of the family Pilocarpaceae[13,28-29].Some taxa withincan be placed in other genera once sufficient evidence obtained in the future molecular studies with larger taxon sampling.Comparisons of the characteristics of the known Chinese species ofare shown in Table 1.

Table 1 Comparisons of the characteristics of the known Chinese species of Fellhanera

TLocalities newly recorded are shown in boldface.

Key to the known Chinese species of

1a.Lichen compounds present, mainly usnic, isousnic and zeorin acids………………………………2

1b.Lichen compounds absent………………………………………………………………3

2a.Ascospores 1-septate, 10-17m×3-6m, 2.5-3.5 times as long as broad………………………………

2b.Ascospores 3(-4)-septate, 10-16m×3-4.5m, 3-4 times as long as broad………………………………

3a.Ascospores l-septate or non-septate………………………………………………………………4

3b.Ascospores more than 1-septate………………………………………………………………5

4a.Ascospores oblong-ovoid, 1-septate, with constriction at septa, 10-16m×4-5m, 2.5-3.5 times as long as broad………………………………

4b.Ascospores elongate ellipsoid, (0-)l-septate, without constriction at septa, (12-)14-17m×3-5.5m, 3-4 times as long as broad………………………………………………………………

5a.Ascospores only 3-septate………………………………………………………………6

5b.Ascospores 5 or 7-septate, rarely 3-septate………………………………………………………………7

6a.Disc orange; ascospores 11-16m×2.5-4.5m, 3.5-4.5 times as long as broad………………………………

6b.Disc reddish brown to dark greyish brown; ascospores 12-18m×3-4m, 4-5 times as long as broad………………………………

7a.Ascospores 5-septate, rarely 3-septate………………………………………………………………8

7b.Ascospores 7-septate, rarely 3 or 5-septate………………………………………………………………9

8a.Ascospores ellipsoid, usually slightly curved and attenuated at one end, 14-21m×3.5-5, 3.5-4.5 times as long as broad

………………………………………………………………

8b.Ascospores oblong, with slight constrictions at septa, 14-26m×3-5, 4-6 times as long as broad………………………………

9a.Ascospores oblong, 7-septate, with constrictions at septa, 18- 24m×3-4.5, 5-6 times as long as broad………………………………

9b.Ascospores clavate, (3-)7-septate, rarely 3 or 5-septate, without constriction at septa, 26-36m×2-3, 10-13 times

as long as broad………………………………

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A Newly Recorded Species offrom China

ZHU Mengli1*, ZHAO Xin1*, WANG Weicheng2,3, JIA Zefeng1**

(1.College of Life Sciences, LiaochengUniversity,Liaocheng 252059, Shandong, China; 2.Institute of Microbiology, Chinese Academy of Sciences,Beijing 100101, China; 3.University of Chinese Academy of Sciences,Beijing 100049, China)

(Vain.) Lücking & Sérus.wasreported as a newly recorded species from China based on the foliicolous lichen specimens collected from Hainan Province.The characteristics of morphology, anatomy and chemistry were provided with microstructure photos.It was characterized by the crustose thallus with light green to orange-red verrucosa, brown apothecia, external exciple prosoplecten- chymatous and narrowly clavate ascospores tapering towards proximal end, (3-)7-septate, 26-36m×2-3m.The phylogenetic tree based on the mtSSU region showed that it had close relationship with the type species(Müll.Arg.) Vězda.A key to known Chinesespecies was also provided.

; Lecanoromycetes; Lichenized fungi; mtSSU; Taxonomy; New record

2021-03-01

2021-04-19

10.11926/jtsb.4398

This work was supported by the National Natural Science Foundation of China (Grant No.31700018; 31800010; 31750001).

ZHU Mengli (Born in 1994), Undergraduate, interesting in taxonomy of lichenous fungi.E-mail: 982340973@qq.com

*Co-first author

Corresponding author.E-mail: zfjia2008@163.com