不同基原藁本藥材的指紋圖譜建立及其化學(xué)計(jì)量學(xué)分析

梁慧 潘曉君 呂渭升 楊文惠 羅宇琴 潘禮業(yè) 霍文杰 李振雨

中圖分類號(hào) R284.1 文獻(xiàn)標(biāo)志碼 A 文章編號(hào) 1001-0408（2021）19-2358-05

DOI 10.6039/j.issn.1001-0408.2021.19.09

摘 要 目的：建立藁本、遼藁本、新疆藁本3種不同基原藁本藥材的超高效液相色譜（UPLC）法指紋圖譜，并進(jìn)行化學(xué)計(jì)量學(xué)分析，為不同基原藁本藥材的鑒別提供參考。方法：采用UPLC法檢測(cè)并結(jié)合《中藥色譜指紋圖譜相似度評(píng)價(jià)系統(tǒng)（2012版）》建立23批不同基原藁本藥材的指紋圖譜，并進(jìn)行色譜峰指認(rèn)和相似度評(píng)價(jià)。采用聚類分析（CA）、主成分分析（PCA）及正交偏最小二乘判別分析（OPLS-DA）等化學(xué)計(jì)量學(xué)分析方法對(duì)不同基原藁本藥材進(jìn)行分析，篩選差異成分。結(jié)果：藁本、遼藁本、新疆藁本藥材的UPLC指紋圖譜中分別標(biāo)定了13、11、11個(gè)特征峰，其中5號(hào)峰為阿魏酸;相似度評(píng)價(jià)結(jié)果顯示，新疆藁本藥材與遼藁本藥材間的相似度為0.312～0.541，新疆藁本藥材與藁本藥材間的相似度為0.324～0.682，藁本藥材與遼藁本藥材間的相似度為0.312～0.671，表明不同基原藁本藥材間的差異較大。經(jīng)CA 、PCA 、OPLS-DA 發(fā)現(xiàn)，不同基原藁本藥材各自聚為一類; 10號(hào)峰、13號(hào)峰、12號(hào)峰、7號(hào)峰、6號(hào)峰所代表的化學(xué)成分為這3種基原藁本藥材的差異成分。結(jié)論：本研究建立了不同基原藁本藥材的UPLC指紋圖譜，篩選出了5種差異成分，可用于鑒別不同基原的藁本藥材。

關(guān)鍵詞 藁本;遼藁本;新疆藁本;基原鑒別;超高效液相色譜法;指紋圖譜;聚類分析;主成分分析;正交偏最小二乘判別分析

Fingerprint Establishment of Rhizoma Ligustici from Different Origins and Their Chemometrics Analysis

LIANG Hui，PAN Xiaojun，LYU Weisheng，YANG Wenhui，LUO Yuqin，PAN Liye，HUO Wenjie，LI Zhenyu（Guangdong Yifang Pharmaceutical Co.， Ltd./Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Formula Granule， Guangdong Foshan 528244， China）

ABSTRACT ? OBJECTIVE： To establish UPLC fingerprint of Ligusticum sinense， Ligusticum jeholense and Conioselinum vaginatium， and to conduct their chemometrics analysis so as to provide reference for the identification of Rhizoma Ligustici from different origins. METHODS： UPLC method combined with Similarity Evaluation System of TCM Chromatographic Fingerprints （2012 edition） were used to establish the fingerprints of Rhizoma Ligustici from different origins. Chromatographic peak identification and similarity evaluation were carried out. Cluster analysis （CA）， principal component analysis （PCA） and orthogonal partial least squares discriminant analysis （OPLS-DA） were performed to analyze Rhizoma Ligustici from different origins， and screen differential components. RESULTS： Totally 13， 11， 11 characteristic peaks were identified in UPLC fingerprints of L. sinense， L. jeholense and C. vaginatium， respectively. Similarity evaluation showed that the similarity between C. vaginatium and L. jeholense were 0.312-0.541; that between C. vaginatium and L. sinense were 0.324-0.682; that between L. sinense and L. jeholense were 0.312-0.671， indicating there was great difference among Rhizoma Ligustici from different origins. CA， PCA and OPLS-DA showed that Rhizoma Ligustici from different origins were clustered into each category respectively; chemical components represented by peak 10， peak 13， peak 12， peak 7 and peak 6 were differential components for Rhizoma Ligustici from 3 origins. CONCLUSIONS： The study establishes UPLC fingerprint of Rhizoma Ligustici from different origins， and screens 5 differential components， which can be used to identify Rhizoma Ligustici from different origins.