Intestinal NK/T cell lymphoma: A case report

Hui Li, Wen Lyu

Abstract

Key words: NK/T cell lymphoma; Indolent T lymphocyte proliferative disease; Crohn's disease; Endoscopy; Misdiagnosis; Case report

INTRODUCTION

In recent years, there have been reports in the literature of NK/T cell lymphoma (NKTCL) originating from the gastrointestinal tract[1]. NKTCL has a low incidence and lacks typical clinical manifestations. The inner lining of the gastrointestinal tract exhibits multiple intestinal mucosa ulcers of different sizes, which are related to Crohn's disease (CD) and indolent T lymphocyte proliferative disease (ITLPD)[2]. It is difficult to identify proliferative diseases, and the tumor cells show extensive variation under microscope. When the infection is complicated, the microscopic findings are often confused with inflammatory infiltration, which results in a high misdiagnosis rate and a difficult diagnosis. We here report a case of an adult female with recurrent fever and diarrhea. CD was diagnosed multiple times by colonoscopy and pathology. The patient was treated with immunosuppressive agents, biological agents, and antibiotics. She had recurrent high fever with diarrhea and weight loss, which became more severe, and she eventually died. Based on consultation of gastroenterologists and pathologists, the diagnosis in this case was considered to be intestinal NKTCL.

CASE PRESENTATION

Chief complaints

A 40-year-old female patient was admitted to the hospital with recurrent fever and loose stools for more than 3 mo and aggravation for 3 d.

History of present illness

The patient had developed fever with loose stools 3 mo ago. She went to a local hospital, and diagnosed with acute gastroenteritis and was discharged after antiinfection, fluid replacement and other symptomatic supportive treatments. Subsequently, the patient repeatedly developed fever and loose stools, which resembled a dilute water sample. The stools were yellow with no blood, and the patient was monitored for signs of emergency. She visited the local hospital many times for treatment, and showed no obvious improvement in her symptoms.

History of past illness

The patient reported no remarkable history of past illness.

Physical examination

Physical examination showed pale mucosa and tenderness of upper abdomen.

Laboratory examinations

The auxiliary examination after admission showed that the white blood cells were 9.9 × 109/L, neutral ratio was 75.7%, hemoglobin was 87 g/L, C-reactive protein was 106 mg/L, and procalcitonin was 3.92 ng/mL. Calprotectin and ANCA IgG were positive. Tests were negative for autoantibody, immunization, blood culture, stool culture, Widal reaction, CMV DNA, and inflammatory bowel disease-associated antibodies.

Imaging examinations

B-ultrasound revealed that multiple lymph nodes in the neck were accessible, including lymph nodes near the root of the neck; and multiple mesenteric nodes were accessible. Colonoscopy revealed multiple colonic ulcers on May 26, 2015 (Figure 1). The colonoscopic biopsy pathology showed chronic mucosal inflammation, interstitial lymphocytes, plasma cells, and infiltration of eosinophils and neutrophils. The patient was administered metronidazole for anti-infection and salford enema fluid combined with Pattersian. The colonoscopic biopsy pathology showed interstitial lymphocyte hyperplasia with erosion and granulation tissue formation, and immunohistochemistry showed chronic inflammation of the sigmoid mucosa on May 29, 2015. The lymphocyte results mainly indicated T lymphocyte hyperplasia. The patient underwent positron emission tomographic-computed tomographic (PET-CT) on June 10, 2015, which showed that18F-fluorodeoxyglucose metabolism in the colon and rectum was increased, the intestinal wall of some segments of the colon was slightly thickened, prompting inflammatory bowel disease, and there were multiple lowmetabolized lymph nodes in the mesenteric root of the abdominal cavity.

Further diagnostic work-up

The patient had a high fever; the highest body temperature was 40.3 oC, the white blood cells were 17.5 × 109/L, and the neutrophil ratio was 91.8%, and meropenem anti-infective treatment was administered. The patient subsequently experienced recurrent high fevers. A pathologist of Sir Run Run Shaw Hospital Affiliated with Zhejiang University was consulted. The expression of CD3, Epstein-Barr virus encoded RNA (EBER), and T-cell intracellular antigen (TIA) was positive; CD20, CD5, CD4, and CD8 were negative; CD56 was suspected to be positive; and Ki-67 was approximately 40%. Pathological diagnosis showed intestinal mucosal focal heterotypic lymphocyte infiltration with an abnormal immune phenotype, and T cell lymphoma could not be ruled out on June 28, 2015. Enteroscopy showed hyperemia and edema of the sigmoid colon mucosa, multiple longitudinal ulcers and proliferative changes of the surrounding mucosa on June 12, 2015 (Figure 2). Bone marrow aspiration showed lymphocytes (accounting for 3.5%, mainly mature) on June 17, 2015. Immunohistochemical analysis on the bone marrow was performed and revealed that CD3, CD2, CD5, CD20, CD79α, myeloperoxidase, and CD61 and CD34 megakaryocytes, were positive, and terminal deoxynucleotidyl transferase was negative on June 18, 2015. Blood tumor immunotyping showed that the proportion of granulocytes increased, which showed abnormal differentiation on the CD15-CD11b and CD16-CD13 dot plot on June 18, 2015. The Ig gene rearrangement test did not detect a monoclonal rearrangement of the gene on June 23, 2015. The hematologist consultation did not consider lymphoma.

FINAL DIAGNOSIS

Colonoscopy showed multiple superficial ulcers. Biopsy showed heterotypic lymphocyte clusters, CD3, and EBER were positively expressed (Figures 3 and 4). Consultation of gastroenterologists and pathologists resulted in a final diagnosis of intestinal NKTCL.

TREATMENT

The patient was administered metronidazole for anti-infection and salford enema fluid combined with Pattersian. The patient had a high fever; the highest body temperature was 40.3 oC, which was managed by meropenem anti-infective treatment. The patient had a recurrent high-grade fever and was diagnosed with severe CD; lymphoma was also considered. And she was given an intravenous injection of methylprednisolone 40 mg for 3 d in combination with Podexan orally and a salford enema on June 6, 2015, which was shifted to metzo at 40 mg orally 3 d later. The patient's temperature gradually stabilized, her diarrhea improved, and the dose of hormone was reduced according to the guidelines.

Figure 1 Colonoscopy revealed a large fecal mass located 25 cm from the anus, and it was impossible to continue with the scope. A: Multiple ulcers of irregular sizes in the colon; B: Ulcers in the colon; C: A large fecal mass in the colon; D: Congestion and edema in the rectum.

OUTCOME AND FOLLOW-UP

After discharge, the patient's temperature gradually became normal, her diarrhea improved, and the hormone dose was reduced to 20 mg orally each day. Subsequently, the patient developed a high-grade fever with significant weight loss. She was diagnosed with “severe CD” in another hospital and was treated with infliximab. The patient achieved a short-term clinical remission. Eventually, the patient relapsed and died a few months later.

DISCUSSION

NKTCL is a rare type of lymphoma with a high degree of malignancy. It is commonly caused by Epstein-Barr virus (EBV) infection, and it occurs more frequently in the middle aged individuals[3]. The median age is 37-47 years. The extra-articular NKTCL occurs most commonly in the gastrointestinal tract, accounting for approximately 30%-50% of all extra-articular NKTCL. The lesion most commonly involves the colon in the intestinal tract, which also involves part of the small intestine[4]. It is estimated that approximately 57.4% of NKTCL cases occur in the colon. Intestinal lesions of adult systemic EBV-positive NKTCL are reported in domestic and foreign literature to have primary clinical features of chronic diarrhea, accompanied by high fever and emaciation; in the early stage of the concurrent blood syndrome or progression to highly malignant lymphoma, the common symptoms of malignant NKTCL are fever, skin rashes, lymph node hyperplasia and hepatosplenomegaly. The adult-onset prognosis is poor, and infection and multiple organ failure are the main causes of death. Symptoms usually last from weeks to months. Endoscopic manifestations are nonspecific, and superficial/erosive, ulcerative/ulcerative infiltrating lesions of the intestinal mucosa are common, and the endoscopic manifestations are variable, which also brings difficulties in diagnosis[5]. EBER was positive by immunohistochemistry, with most patients expressing CD3, CD56, TIA-1, and occasionally CD4 (0%-11%) or CD8 (0%-20%)[4,6,7]. TR clonal rearrangements occur in 17%-40% of NKTCL patients[8-10]. Gene array in lymphoma revealed that the most common chromosomal region deletions were 6q, 8p, 11q, 13q, and 17p. The most common chromosomal regions were 1q, 17q, and 20q, and the smallest chromosomal deletion region was 6q, which was seen in 40%-50% of cases. This region contained tumor suppressor genes such asprdm1, foxo3, andhace1[11-15]. Second-generation sequencing of nasopharyngeal ENKTCL tissues detected mutant genes (jak3, stat3, stat5b), epigenetic regulators (bcorandmll2), and DNA damage repair genes (tp53, ddx3x) in the JAK-STAT pathway[3,16]. NKTCL has no typical clinical features and no effective treatment is available. Cyclophosphamide, doxorubicin, vincristine, and prednisone (CHOP) chemotherapy is currently recommended, but patients can only achieve short-term clinical remission and prognosis is poor, with a median survival of 7 mo. EBV infection also predicts a worse prognosis[3,8].

Figure 2 Colonoscopy showed hyperemia and edema of the sigmoid colon mucosa; multiple longitudinal ulcers were seen, with hyperplasia-like changes in the surrounding mucosa. A: Ulcers and mucosal hyperplasia in the sigmoid; B: Ulcers and mucosal hyperplasia in the sigmoid; C: Ulcers and mucosal hyperplasia in the sigmoid; D: Ulcers and mucosal hyperplasia in the sigmoid.

Figure 3 Colonoscopic biopsy findings: intestinal mucosal focal heterotypic lymphocyte infiltration. A, B and C: Biopsy showed intestinal mucosal focal heterotypic lymphocyte infiltration, B and C were enlargements of a partial area in A; D, E and F: Biopsy showed intestinal mucosal focal heterotypic lymphocyte infiltration, E and F were enlargements of a partial area in D. Hematoxylin and eosin staining.

Figure 4 Immunohistochemical results: the expression of CD3 and Epstein-Barr virus encoded RNA was positive. The expression of CD20, CD4 and CD8 was negative, and Ki-67 was approximately 40%.

ITLPD is a disease with indolent behavior. This lymphocytic proliferative disease has a rare clinical onset, and due to atypical clinical symptoms, pathological diagnosis is difficult, and the misdiagnosis rate is high. ITLPD is classified as a type of lymphoma in the fourth edition of the WHO classification, and its occurrence is rare. At present, the etiology of ITLPD is not clear, and some patients have a history of inflammatory bowel disease (IBD) and autoimmune diseases[17,18]. The clinical symptoms of ITLPD occurring in the gastrointestinal tract are nonspecific, including abdominal pain, nausea and vomiting, diarrhea, and gastrointestinal bleeding, which are often misdiagnosed as CD. The onset age of CD ranges from 15 to 77 years (the median age is 51 years). Ten patients diagnosed with ITLPD have been reported. The ten patients were young and middle-aged. The patients’ main clinical features were abdominal pain and diarrhea. Six of the patients had endoscopic lesions involving the large intestine, and some patients had lesions involving the whole digestive tract, characterized by shallow mucosal ulcers or multiple small polyps that could be involved in colonic lesions. It is difficult for colonoscopy to identify IBD. Colonoscopy usually shows intestinal mucosal ulcers, nodular hyperplasia, polyps, and mucosal erosion. Microscopically, small-to medium-sized mature lymphocytes in the mucosal lamina propria can be seen. In some cases, lymphocytes can be seen with mild atypia. Clusters of lymphocytes (lymph cytopathic lesions) are seen in the crypt epithelium. Biopsy molecular expression characteristics showed that CD3 was positively expressed in all 10 patients, and eight patients were CD4-, CD8+, and TIA+; one patient was CD4-/CD8-; and one patient was CD4-/CD8-. Another study reported a case of CD4-/CD8- ITLPD, which was characterized by abdominal pain and diarrhea. The patient had a history of CD and died within 1 year after receiving 5 courses of CHOP chemotherapy. ITLPD with CD4-/CD8- cells is rare, there is no effective treatment, and the average survival of patients is short. ITLPD can affect the whole digestive tract. The histological diagnosis is that the mucosa or (and) lamina propria infiltrates small and single lymphocytes. In some cases, lymphocytes may infiltrate into submucosal tissue, usually not covering the entire tissue layer nor forming lumps. Lesions may be continuous or multifocal, with variable immunophenotypes.

NKTCL may be misdiagnosed as CD. CD is a chronic intestinal mucosal inflammatory change and dysfunction of the intestinal epithelium and is a comprehensive disease. The etiology of CD is unclear, and it can be induced by genetic susceptibility, immune abnormalities, the environment, and changes in the intestinal microenvironment. The typical clinical features of CD are chronic diarrhea, abdominal pain, emaciation, and malnutrition[19]. Patients with CD concurrent with septicemia may have high fever and it is difficult to identify NKTCL. Typical laboratory tests of CD include thrombocytosis, anemia, and hypersensitive C-reactive protein. Fecal calprotectin is an important CD screening and activity evaluation index. Endoscopy is still an important examination method. Endoscopic changes of typical CD include segmental intestinal inflammation, longitudinal ulcers, and mucosal changes in visible pebble samples. The pathological biopsy showed no caseating granuloma change and no atypical lymphocyte infiltration. Noncaseous granuloma formation is one of the diagnostic criteria for Crohn's disease,with only a 15% positive rate of endoscopic biopsy, and a positive rate of 70% in surgical biopsy specimens. Digestive endoscopy combined with pathological biopsy is an important basis for the diagnosis of CD. In addition, capsule endoscopy is also a safe and noninvasive method for intestinal examination, which can observe early intestinal mucosal lesions. The prognosis of CD is relatively good, but there is still a lack of effective radical treatment at present[20]. Drug therapy is mostly used to control disease activity and maintain disease remission, and prevent complications and intestinal damage. After remission using biological agents, maintenance therapy can be continued or immunosuppressant maintenance therapy can be used[21]. If drug therapy fails or complete intestinal obstruction, acute perforation, uncontrolled massive bleeding and other serious complications occur, surgical treatment can be performed[22].

The relationship between NKTCL, CD, and ITLPD is unclear[17,23,24]. It is usually difficult to distinguish them based on clinical symptoms. The common symptoms are similar. The general characteristics, such as age of onset, can also overlap, and the endoscopic features are usually nonspecific. Some NKTCL and ITLPD patients have a history of CD, and some ITLPD patients may have lymphoid follicular hyperplasia and granulomas. Histopathology of gastrointestinal tract derived ITLPD may show inflammatory changes, and most cases are initially misdiagnosed as inflammatory diseases. Significant lymphocytic infiltration or single lymphocytic infiltration in the lamina propria helps to distinguish ITLPD from CD. ITLPD is an inert disease with slow disease development and usually with mild clinical symptoms. In addition to typical cell and tissue morphological differences, the lack of epithelial cell characteristics, rare mitosis, and low cell proliferation activity can be diagnosed as ITLPD. NKTCL has a relatively high degree of malignancy. NKTCL is invasive and severe and usually has a shorter median survival than ITLPD and CD[6,25]. NKTCL seen under the microscope is easily confused with CD and ITLPD with respect to histological phenotypes. Hormone and pedestrian therapy is effective for CD, while hormone therapy can also clinically relieve NKTCL and ITLPD in the short term, which increases the difficulty of diagnosis. The high degree of overlap among these three diseases, including clinical symptoms, endoscopy, and pathology, is the main reason for the difficulty in diagnosis. Pathological diagnosis is still the gold standard. It requires that multiple end-point biopsies be taken during the endoscopic biopsy, and the diseased tissue is excavated if necessary. At the same time, it is necessary to closely integrate clinical symptoms and pathological characteristics and perform a comprehensive evaluation with gene sequencing if necessary, so as to establish a correct diagnosis.

The clinical manifestations of the present patient were characterized by chronic diarrhea and repeated fever. B-ultrasound and PET-CT showed multiple lymph node enlargement. Several analyses of the endoscopic biopsy pathology suggested T lymphocyte hyperplasia, and heterotypic lymphocytes were visible. Immunohistochemistry showed positive CD3 and EBER expression. Digestive physicians and pathologists diagnosed NKTCL after several discussions. This disease is rare in adults, the prognosis of patients is poor, and the mortality rate is high. At present, the disease is not well understood, so early diagnosis and treatment are particularly important. Due to the lack of typical clinical symptoms and colonoscopic manifestations, the biopsy tissue under colonoscopy is small and shallow, and early lymphocyte aberration is not obvious, which often makes it difficult to distinguish this disorder from other inflammatory bowel diseases and intestinal lymphoproliferative diseases. At present, there is no unified treatment of NKTCL. Some patients receiving chemotherapy can achieve short-term remission, but cannot reduce the viral load in the body. Antiviral and immunosuppressive treatments may have short-term effects. In this case, hormone therapy was given several times, and short-term clinical remission was obtained, but long-term clinical remission was unsuccessful.

CONCLUSION

We reported a case of malignant intestinal NKTCL, which is a rare type of lymphoma and is hard to distinguish from CD, ITLPD, and other immune disorders. The development of medicine, biology and computer technology has enhanced our understanding of these diseases, including a more detailed and comprehensive classification of this type of immune disorder. However, the etiology and origin of this disease are still unclear. More potential molecular markers need to be explored. The molecular regulatory network needs to be supplemented to help with the differential diagnosis. Individualized treatment plans need to be formulated to improve the overall survival of patients. Clinicians and pathologists need to improve their awareness of this disease and reduce the rate of misdiagnosis in order to achieve better clinical management of patients with this disease.