• <tr id="yyy80"></tr>
  • <sup id="yyy80"></sup>
  • <tfoot id="yyy80"><noscript id="yyy80"></noscript></tfoot>
  • 99热精品在线国产_美女午夜性视频免费_国产精品国产高清国产av_av欧美777_自拍偷自拍亚洲精品老妇_亚洲熟女精品中文字幕_www日本黄色视频网_国产精品野战在线观看 ?

    Molecular Markers and Candidate Genes for Thermo-Sensitive Genic Male Sterile in Rice

    2019-05-23 01:10:24SudthanaKhlaimongkhonSriprapaiChakhonkaenKeasineePitngamKhanitthaDitthabNumphetSangarwutNatjareePanyawutThiwawanWasinanonChareeratMongkolsiriwatanaJulaparkChunwongseAmorntipMuangprom
    Rice Science 2019年3期

    Sudthana Khlaimongkhon, Sriprapai Chakhonkaen, Keasinee Pitngam, Khanittha Ditthab, Numphet Sangarwut,Natjaree Panyawut, Thiwawan Wasinanon, Chareerat Mongkolsiriwatana, Julapark Chunwongse, Amorntip Muangprom,

    ?

    Molecular Markers and Candidate Genes for Thermo-Sensitive Genic Male Sterile in Rice

    Sudthana Khlaimongkhon1, Sriprapai Chakhonkaen2, Keasinee Pitngam2, Khanittha Ditthab2, Numphet Sangarwut2,Natjaree Panyawut2, Thiwawan Wasinanon2, Chareerat Mongkolsiriwatana3, Julapark Chunwongse1, Amorntip Muangprom1, 2

    (Center for Agricultural Biotechnology, Kasetsart University, Nakhon Pathom 73140, Thailand; National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani 12120, Thailand; Division of Genetics, )

    The discovery of thermo-sensitive genic male sterility (TGMS) has led to development of a simple and highly efficient two-line breeding system. In this study, genetic analysis was conducted using three F2populations derived from crosses between IR68301S, anTGMS rice line, and IR14632 (), Supanburi 91062 () and IR67966-188-2-2-1 (), respectively. Approximately 1:3 ratio between sterile and normal pollen of F2plants from the three populations revealed that TGMS is controlled by a single recessive gene. Bulked segregant analysis using simple sequence repeat (SSR) and insertion-deletion (InDel) markers were used to identify markers linked to thegene. The linkage analysis based on the three populations indicated that thelocus was located on chromosome 2 covering the same area. Using IR68301S × IR14632 F2population, the results showed that thelocus was located between SSR marker RM12676 and InDel marker 2gAP0050058. The genetic distance from thegene to these two flanking markers were 1.10 and 0.82 cM, respectively. InDel marker 2gAP004045 located between these two markers showed complete co-segregation with the TGMS phenotype. In addition, InDel marker vf0206114052 showed 2.94 cM linked to thegene using F2populations of IR68301S × Supanburi 91062. These markers are useful tool for developing new TGMS lines by marker-assisted selection. There were ten genes located between the two flanking markers RM12676 and 2gAP0050058. Using quantitative real-time PCR for expression analysis, 7 of the 10 genes showed expression in panicles, and response to temperatures. These genes could be the candidate gene controlling TGMS in IR68301S.

    hybrid rice; thermo-sensitive genic male sterility; insertion/deletion; simple sequence repeat; marker-assisted selection

    Rice (L.) is one of the most important global food crops.The world’s population has doubled since the early 1960s, therefore, existing yields of the major cereal crops will be insufficient to meet the food needs of the future (FAO, 2018). In addition, arable lands have declined worldwide, thus an increase in yield will be necessary to meet this demand (Bruinsma, 2003). Rice production in Thailand signifies a portion of the Thai economy and is a major export product.However,yield of Thai rice is less than that of the neighboring countries such as Indonesia, Vietnam, Malaysia, Laos and the Philippines (USDA, 2016). Yields have increased with the development of hybrid rice currently planted in many countries. The trend of hybrid rice development in both commercialization and research of private companies has been increasing. Hybrid rice technology has increased yields by 20%–30% under unchanged irrigation conditions (Yuan, 1998; Virmani, 2003). Hybrid rice seeds of large scale commercial production have been released to the market in China since 1976. The Ministry of Agriculture of China develops super rice breeding programs to increase rice yield per planting area, resulting thataverage rice yieldsin China rose from 1.89 t/hm2in 1949 to 6.71 t/hm2in 2012 (Cao and Zhan, 2014). In Thailand, average rice yield is only 2.89 t/hm2in 2018 (Thai Rice ExporterAssociation, 2018). Many countries which adopt hybrid rice technology are able to achieve higher yields.

    Hybrid rice seed production involves the use of male sterility systems. Two well established male sterility systems in rice are cytoplasmic genetic male sterility (CMS), a three-line system, and environmentally sensitive genic male sterility (EGMS), a two-line system including photoperiod-sensitive genic male sterility (PGMS) and thermo-sensitive genic male sterility (TGMS). The discovery of PGMS and TGMS has led to development of a simple and highly efficient two-line breeding system in hybrid rice seed production. Several EGMS-related genes have been mapped such as(Wang et al, 1995),(Yamaguchi et al, 1997; Pitnjam et al, 2008),(Subudhi et al, 1997;Lang et al, 1999),(Dong et al, 2000),(Wang et al, 2003;Nas et al, 2005; Jiang et al, 2006; Yang et al, 2007),(Lee et al, 2005),(t) (Li et al, 2005),(Hussain et al, 2011),(Sheng et al, 2013),(Qi et al, 2014),(Peng et al, 2010),(Liu et al, 2001; Zhou et al, 2011),(Zhanget al,1994),(Dinget al,2012a, b),(Xu et al, 2011),(Zhou et al, 2012),(Peng et al, 2008) and(Jia et al, 2001). Candidate genes for EGMS have been reported, Myb-like DNA-binding domain containing protein (Zhou et al, 2011), a nuclear ribonuclease Z gene (Xu et al, 2011; Zhou et al, 2014), ORMDL (Pitnjam et al, 2008; Chueasiri et al, 2014) and the putative pollen specific protein encoded by(Nas et al, 2005). In addition, several genes controlling male sterility phenotype have been reported in rice such as undeveloped() (Jung et al, 2005), tapetum degeneration retardation () (Li et al, 2006), CER-like protein causing wax-deficient() (Jung et al, 2006), and LRR receptor kinase affecting(Nonomura et al, 2003).

    IR68301S is a TGMS rice line obtained from International Rice Research Institute (IRRI), the Philippines. Male sterility in IR68301S is stable in a high temperature (≥ 28oC), and it has been used for the development of two-line hybrid in Thailand. In this study, we mapped a gene controlling TGMS in IR68301S, and identified candidate genes using expression analysis. The knowledge in this study will be useful for rice breeding programs.

    Materials and methods

    Rice materials and phenotypic screening

    An F2population, generated from a cross between IR68301S (B2), anTGMS mutant rice line, and IR14632 (B30), arice line, was used for identification of linked markers to thegene. These plants were obtained from IRRI. In addition, the other F2populations generated from crosses between B2 and Supanburi 91062 (B11), a Thairice line having high yield, and between B2 and IR67966-188-2-2-1(KM9), arice line, were also used. Theandcrosses were used to increase chances for polymorphic marker identification, and then these markers were used for linkage analysis. The F2populations were planted in paddy fields for observation on pollen sterility/fertility in summer of 2011, 2012 and 2013, in which the maximum temperatures were 33 oC–37 oC. Pollen sterility and fertility of the F2plants were evaluated on flowering day by observing anthers with the naked eyes and under a microscope after staining with 1% I2-KI solution. The χ2test was applied to determine the F2segregation ratio of sterility and fertility.

    Genotyping and linkage analysis

    Genomic DNA was extracted from fresh leaves of individual F2sterile plants generated from all the three crosses using the cethy trimethylammonium bromide (CTAB) method (Murray and Thomson, 1980). Polymorphic markers between parents and bulked segregant analysis (BSA) using pooled DNA from 10 individuals each for fertile and sterile plants, were applied to identify linked markers togene. The resulting linked markers were used to determine the genotype of the individual F2plants. Primers for single nucleotide polymorphism (SNP), and insertion-deletion (InDel) markers were designed from sequences obtained from GRAMENE database (http://www.gramene.org) andRiceVarMap (http://ricevarmap.ncpgr.cn/).Amplification of DNA fragments using simple sequence repeat (SSR) and InDels was performed as previously described by McCouch et al (2002) and Pitnjam et al (2008). The resulting PCR products were electrophoretically separated on 3% agarose gel or 6% acrylamide gel, and DNA patterns were observed by ethidium bromide or silver staining.The recombination frequency () was calculated with the formula:

    = (1+2)/

    whereis the total number of sterile plants surveyed,1is the number of sterile individuals with the homozygous band of the fertile parent, and2is the number of individuals with heterozygous bands (Peng et al, 2010). Recombination frequency was converted into genetic distance (cM) for linkage analysis.

    Expression analysis

    Segregating wild type male fertile and TGMS male sterile BC3F5plants generated from Pathum Thani 1, a Thai elite line, crossed with B2 were used for expression analysis, These plants were grown under natural condition at National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency (14o04′50.6′′N and 100o36′09.0′′E), Pathum Thani Province, Thailand. Pre-germinated grains wereseeded in a tray and then the seedlings were transplanted into plastic pots (without hole at the bottom, diameter of 20.5 cm and height of 16.0 cm) with one seedling per pot. About one month before flowering, each of at least five plants/line were moved to controlled growth rooms at temperatures(24 ± 2) oC and (32 ± 2) oC under 80% relative humidity, 12 h light/12 h darkness until flowering. For expression analysis, young panicles about 13?14 cm in length (dyad stage) were harvested from BC3F5wild type and TGMS mutant plants at the same day. Total RNA was extracted using TRIZOLTMreagent (Invitrogen, USA). Quantitative real-time PCR (qRT-PCR) experiments were performed with cDNA synthesis kit (Fermentus, Lithuania) using cDNAs transcribed from total RNA. Based on information in GRAMENE database, genes located between two flanking markers tightly linked togene were used for expression analysis by qRT-PCR.The qRT-PCR was performed using Bio-RAD iCycler iQ5 Machine (BioRAD, USA) and all reactions were conducted in 96-well plates (BioRad, USA). The qPCRBIO SyGreen Mix Lo-ROX (PCR Biosystems) assay was used in a total volume of 10 μL per reaction. Each reaction mixture contained 1 μL cDNA, 2μL distilled water, 1 μL (10 μmol/L) forward primer, 1 μL (10 μmol/L) reverse primer and 5 μL of 2× qPCRBIO SyGreen Mix. All qRT-PCR plates were carried out with following cycling condition: 95 oC for 3 min, following 35 cycles of 95 oC for 30 s, 57 oC to 63 oC for 30 s and 72 oC for 30 s, then 95 oC for 1 min and a melting curve from 60 oC to 95 oC in 0.5 oC increments.was used as an internal control. Three replications were performed for all reactions. The 2?ΔΔCtmethod was used to calculate relative gene expression(Livak and Schmittgen, 2001). The significantly of qRT-PCR were calculated byprogram (http://cran.r-project.org/)with ANOVA and Duncan’s new multiple range test.

    Results

    Pollen staining

    Mature anthers of the TGMS mutant and wild type grown in controlled growth rooms under fertile and sterile conditions were observed. There was no pollen produced in anthers of TGMS mutant plants under sterile condition (32 ± 2) oC (Fig. 1-A), while underthe fertile condition of (24 ± 2) oC, pollen production was similar to that of wild type plants (Fig. 1-B). At these two conditions, wild type plants produced normal pollen (Fig. 1-C and -D).

    Fig. 1. Pollen staining of thermo-sensitive genic male sterility (TGMS) mutant and wild type.

    A, Abortive anther of the TGMS mutant grown under high temperature (32 ± 2)oC. B, Normal anther of the TGMS mutant grown under low temperature (24 ± 2) oC. C, Normal anther of wild type grown under high temperature (32 ± 2) oC. D, Normal anther of wild type grown under low temperature (24 ± 2) oC.

    Genetic analysis of tms gene controlling TGMS in IR68301S

    By growing the F2populations under sterile condition of(32 ± 2) oC, the segregation patterns of fertile to sterile plants in the F2populations were near to 3:1 ratio in all the three populations (Table 1). The results indicated that the TGMS of IR68301S was controlled by a single recessive gene.

    Mapping of tms gene controlling TGMS in IR68301S

    A total of 119 SSR, 10 SNP and 283 InDel markers were used to identify polymorphism between parents from the three crosses. The results showed that 53, 3 and 7 markers were polymorphic between the parents from B2 × B30, B2 × B11 and B2 × KM9, respectively.

    The resulting polymorphic markers were applied to identify linked markers to thegene using BSA. And 24 out of the 53 markers showed linkage to thegene in F2male sterile plants from B2 × B30. In addition, three and four markers showed linkage to thegene in F2male sterile plants from B2 × B11 and B2 × KM9, respectively (Supplemental Table 1). According to GRAMENE and RiceVarMap databases, these markers are on chromosome 2 at the position of 4.19 to 7.44 Mb.

    Table 1. Segregation patterns of F2 populations for fertility and sterility.

    B2, IR68301S; B30, IR14632; B11, Supanburi 91062; KM9, IR67966-188-2-2-1.

    The value of χ20.05is 3.84.

    The linked markers identified from each cross were used to genotype the F2male sterile. These F2male sterile plants selected from flowering plants in summers (33 oC–37 oC) were completely sterile. A total of 217, 259 and 450 F2male sterile plants from B2 × B30, B2 × B11 and B2 × KM9 were used for genotyping, respectively. Segregation patterns were shown in Fig. 2. A linkage analysis based on the three F2populations showed that thegene in IR68301S was located on chromosome 2 (Fig. 3). Using F2sterile individuals of B2 × B30, the results showed that thegene was located between SSR marker RM12676 at 5.74 Mb and InDel marker 2gAP0050058 at 5.81 Mb. The genetic distance of thegene from the two markers was 1.10 and 0.82 cM, respectively. In addition, InDel marker 2gAP004045 showed complete co-segregation with the TGMS phenotype (Fig. 3-A). Using F2sterile individuals of B2 × B11, the results showed thatgene was located between RM126016 at 4.69 Mb and InDel marker vf0206114052 at 6.11 Mb. The genetic distance of thegene from the two markers was 14.97 and 2.94 cM, respectively (Fig. 3-B). Using F2population of B2 × KM9, the results showed that four SSR markers, RM126016, RM12649, RM6378 and RM12674 were linked to thegene. The genetic distance of thegene from these markers were 12.50, 8.75, 8.25 and 6.25 cM, respectively (Fig. 3-C).

    Expression analysis of candidate genes located inside the flanking markers

    Based on information in GRAMENE database, there are ten genes (Table 2) located between the two flanking markers, RM12676 and 2gAP0050058. Nine out of the ten genes were annotated as expressed proteins, and one was annotated as a conserved hypothetical protein. These genes were tested for expression in panicles of wild type and TGMS mutantplants under fertile and sterile conditions. The results showed that the expressions of seven genes were detected in the tested tissues, but those of,andcould not be detected (data not shown). All the seven expressed genes showed different expression levels under fertile and sterile conditions or between the TGMS mutant and the wild type. Genes,andshowed significantly different expression levelsbetween the two conditions in the TGMS rice plants but no significantly different expression levels were detected between the two conditions in the wild-type rice plants (Fig. 4).andshowed higher expression levelsunder fertile conditionthan sterile condition, whileshowed higher expression levelsunder sterile conditionthanthe fertile conditionsThese three genes showed higher expression levels in the TGMS mutants than in the wild type under both conditions. These two genesandshowed a similar pattern of expression both in the mutant and wild-type rice plants, by showing higher expression under lower temperature condition. These genesandshowed a similar pattern of expression by showing higher expression under lower temperature condition in the TGMS mutants, but higher expression under higher temperature condition in wild-type plants (Fig. 4).

    Fig. 2. Samples of genotyping F2male sterile plants.

    P1, Female parent (IR68301S, B2); P2, Male parent (IR14632, B30); 1–10, F2male sterile individuals of B2 × B30 (×) using Os02g12370 marker; P3, Male parent (Supanburi 91062, B11); 11–20, F2male sterile individuals of B2 × B11 (×) using vf0206114052 marker.

    Fig. 3. Genetic linkage map of thermo-sensitive genic male sterility gene on chromosome 2.

    The linkage maps were analyzed based on male sterile F2populations of IR68301S × IR14632 (A), IR68301S × Supanburi 91062 (B) and IR68301S × IR67966-188-2-2-1 (C). Distances of each marker in centiMorgans (cM) fromgene were given on the left side of the genetic map.

    Table 2. Genes located between the two flanking markers RM12676 and 2gAP0050058.

    Fig. 4. Relative expression levels of genes in panicles by quantitative real-time PCR.

    HT, High temperature condition (32 oC); LT, Low temperature condition (24 oC); TGMS, Thermo-sensitive genic male sterility.

    Data represent Mean ± SE, and the same lowercase letter(s) indicate no significant difference at< 0.05 by Duncan’s new multiple range test.

    Discussion

    The segregation patterns of fertile to sterile plants in the three F2populations followed the 3:1 ratio, which is typical of Mendelian low, indicating that the TGMS of anIR68301S is controlled by a single recessive gene, which is in agreement with other studies (Borkakati and Virmani, 1996; Lopez et al, 2003; Wang et al, 2003; Hussain et al, 2011; Qi et al, 2014).

    Using the three mapping populations, thegene in IR68301S was mapped on chromosome 2. Using B2 × B30 F2population, thegene was located between SSR marker RM12676 and InDel marker 2gAP0050058. In addition, InDel marker 2gAP004045, located between these two markers, showed complete co-segregation with the TGMS phenotype. These markers are useful tool for developing new TGMS lines by marker-assisted selection (MAS) and identifying the TGMS individuals at earlier stages of line development in rice breeding program. Using F2population fromparents (B2 and B11),only two markers were linked to thegene. The genetic distance of thegene from the two markers was 14.97 and 7.50 cM. Since major subspecies of rice in Thailand is, the polymorphic and tightly linked markers generated fromparents will be more practical than the linked markers generated from×crosses. Therefore, several markers nearby the flanking region were designed and test for polymorphism between the twoparents (B2 and B11). The 30 InDel markers covering flanking region from 5.50 to 6.28 Mb were designed to test polymorphism between these two parents (Supplemental Table 2). The results showed that only vf0206114052 at 6.11 Mb showed polymorphism and linked (2.94 cM) to thegene. Therefore, this marker will be useful inbreeding programs. The two tightly linked InDel markers, vf0206114052 and 2gAP004045, will be useful for×and×rice breeding programs, respectively.

    Candidate genes for EGMS have been reported. The locus of(photo period-sensitive male sterility) and(photo- or thermo-sensitive genic male sterility) represent the same locus on chromosome 12 conferring PGMS and TGMS traits (Zhou et al, 2012). This report revealed that a SNP C-to-G between the fertile lines and sterile lines led to increasing of methylation in putative promoter region of this non-coding gene, resulting in premature programmed cell death (PCD) in developing anthers, thus causing PGMS inand TGMS insubspecies.

    Theis a candidate gene ofwhich locates on chromosome 2 at the position of 6.39 Mb (htpp://www.gramene.org) and this gene encodes RNase ZS1. Experimental results revealed that at high temperature RNase ZS1loses its function and causes defective pollen production (Zhou et al, 2014). This enzyme maintained mRNAs ofUbat normal level and led to male fertility.LOC_Os02g12290, a nuclear ribonuclease Z gene was identified as the candidate for thegene, located on chromosome 2 in Guangzhan 63S, aPTGMS line (Xu et al, 2011).

    The gene controlling TGMS in IR68301S was located between the two flanking markers at the position of 5.74 to 5.81 Mb. There were no previously reported EGMS genes at this position. There were 10 genes located between the flanking markers, however, we detected the expressions level of only 7 genes in the TGMS mutant and the wild type under both conditions. All these genes responded to temperatures by showing different expression levels under fertile and sterile conditions in the TGMS rice plants.Threegenes,andshowed similar expression levelsunder fertile and sterile conditions in wild type rice plants but they showed significantly different levels of expression in the mutant plants under sterile condition. Interestingly,showed significantly higher expression level under sterile condition than under fertile condition in the TGMS rice plants, while it showed similar expression levelsunder both conditions in the wildtype rice plants. Theencodesa tetratricopeptide-like helical domain protein (TPR). Shin et al (2014) reported that the N-terminal tetratricopeptide repeat1 (TPR1) domain is essential for its interaction with pectate lyase-like proteins (PLLs) in petunia, maize and. Lacking the TPR1 domain, no interaction resulting in pollen that failed to germinate.pollen calmodulin-binding protein (OsPCBP) is a Ca2+-dependent calmodulin-binding (CaMBP) protein and it contains six TPR motifs that can interact with protein in pollen to regulate germination and also involve in starch accumulation. The absence or reduction of starch can cause abortive pollen (Zhang et al, 2012). This information indicated that the TPR domain is associated with pollen development.encodes a zinc ion binding protein andencodes a zinc finger protein. The zinc finger family proteins are transcription factors (TF) involved in abiotic stress. Bai et al (2015) reported that three zinc finger TF genes (LOC_Os06g14180, LOC_Os12g37800, LOC_Os11g14000) are up-regulated and two zinc finger TF genes (LOC_Os01g50750 and LOC_Os05g44550) are down-regulated following cold stress treatment in rice male sterile lines. In, the result of RNA sequencing showed that, a zinc finger TF protein gene associated with flowering time and tolerance to abiotic stress (Yang et al, 2014). Yang et al (2015) reported that zinc finger CCCH domain-containing proteins are two (Unigene43080_Zhong-531and Unigene43085_Zhong-531) of differential expression genes (DEGs) which respond to temperature interacting with nitrogen at meiosis stage of rice spikelet. In our study,andshowed higher expression levels under lower temperature in the TGMS plants, while in wild type rice plants,showed lower expression levelsunder lower temperature andshowed similar expression levels under both conditions.encodes an F-box domain and kelch repeat containing protein.F-box proteins are critical for degradation of cellular proteins. The F-box protein-encoding genes have specific and/or overlapping expression during floral transition as well as panicle and seed development. The F-box protein encoding genes are involved in different abiotic stress conditions (Jain et al, 2007; Chunthong et al, 2017). The TPR domains were predicted in rice F-box proteins responsible for processing and/or translation of mRNAs. DNA-binding domains such as zinc finger, ring finger and helix loop helix are also found in rice F-box proteins, which may be directly or indirectly involved in transcriptional regulation (Small and Peeters, 2000).

    encodes a conserved hypothetical protein. It showed higher level of expression under the higher temperature condition than under the lower temperature condition in the wildtype rice plants, but in the TGMS rice plants the expression of this gene was similar under both conditions.encodes a putative uncharacterized protein. It showed similar expression patterns in the TGMS and wild type rice plants but the expression levels were higher in the TGMS than in wild type rice plants under both conditions. Although the functions of,andare not clear but their expressions in our study suggested that they could be involved in TGMS. Therefore, further study is needed to identify the candidate gene controlling TGMS in IR68301S rice line.

    Acknowledgements

    This study was supported by Center for Agricultural Biotechnology, Kasetsart University, Center of Excellence on Agricultural Biotechnology (AG-BIO/PERDO- CHE), Agricultural Research Development Agency (ARDA), and National Science and Technology Development Agency, Thailand. The authors thank Dr. Peera Jaruampornpan for her critical reading and comments on the manuscript.

    supplemental datA

    The following materials are available in the online version of this article at http://www.sciencedirect.com/science/ journal/16726308; http://www.ricescience.org.

    Supplemental Table 1. Markers on chromosome 2 linked to thegene using F2male sterile plants from IR68301S × IR14632, IR68301S × Supanburi 91062 and IR68301S × IR67966-188-2-2-1.

    Supplemental Table 2. InDel markers on chromosome 2 covering flanking region from 5.50 to 6.28 Mb.

    Bai B, Wu J, Sheng WT, Zhou B, Zhou LJ, Zhuang W, Yao DP,Deng QY. 2015. Comparative analysis of anther transcriptome profiles of two different rice male sterile lines genotypes under cold stress., 16(5):11398–11416.

    Borkakati R R, Virmani SS. 1996. Genetics of thermosensitive genic male sterility in rice.,88(1): 1–7.

    Bruinsma J. 2003. World Agriculture: Towards 2015/2030. London, UK: Earthscan.

    Cao L Y, Zhan X D. 2014. Chinese experiences in breeding three-line, two-line and super hybrid rice.: Yan W G, Bao J S. Rice: Germplasm, Genetics and Improvement. Intech: 279–308.

    Chueasiri C, Chunthong K, Pitnjam K, Chakhonkaen S,Sangarwut N,Sangsawang K,Suksangpanomrung M,Michaelson LV,Napier JA,Muangprom A. 2014. Rice ORMDL controls sphingolipid homeostasis affecting fertility resulting from abnormal pollen development.,9(9): e106386.

    Chunthong K, Pitnjam K, Chakhonkaen S, Sangarwut N, Panyawut N, Wasinanon T, Ukoskit K, Muangprom A. 2017. Differential drought responses in F-box gene expression and grain yield between two rice groups with contrasting drought tolerance.,36:970–982.

    Ding J H, Lu Q, Ouyang Y D, Mao H L,Zhang P B,Yao J L,Xu C G,Li X H,Xiao J H,Zhang Q F. 2012a. A long noncoding RNA regulates photoperiod-sensitive male sterility, an essential component of hybrid rice.,109(7):2654–2659.

    Ding J H, Shen J Q, Mao H L, Xie W B, Li X H,Zhang Q F. 2012b.RNA-directed DNA methylation is involved in regulating photoperiod-sensitive male sterility in rice.,5(6):1210–1216.

    Dong NV, Subudhi PK, Luong PN, Quang VD, Quy TD, Zheng HG, Wang B, Nguyen HT. 2000. Molecular mapping of a rice gene conditioning thermo-sensitive genic male sterility using AFLP, RFLP and SSR techniques.,100(5):727–734.

    FAO. 2018.FAO Statistical Pocketbook 2018: World Food and Agriculture. Rome: FAO.

    Hussain A J, Ali J, Siddiq EA, Gupta VS, Reddy UK, Ranjekar PK. 2011. Mapping ofgene for temperature-sensitive genic male sterility (TGMS) in rice (L.).,131(1):42–47.

    Jain M, Nijhawan A, Arora R, Agarwal P, Ray S, Sharma P, Kapoor S, Tyagi AK, Khurana JP. 2007. F-Box proteins in rice: Genome-wide analysis, classification, temporal and spatial gene expression during panicle and seed development, and regulation by light and abiotic stress.,143(4):1467–1483.

    Jia JH, Zhang DS, Li CY, Qu XP, Wang SW, Chamarerk V, Nguyen HT, Wang B Y. 2001. Molecular mapping of the reverse thermosensitive genic male-sterile gene () in rice.,103(4):607–612.

    Jiang D G, Lu S, Zhou H, Wu X J, Zhuang C X, Liu Y G, Mei M T. 2006. Mapping of the rice (L.) thermo-sensitive genic male sterile genewith EST and SSR markers.,51(4):417–420.

    Jung KH, Han MJ, Lee YS, Kim YW,Hwang I,Kim MJ,Kim YK,Nahm BH,An G. 2005. Rice undevelopedis a major regulator of early tapetum development.,17(10):2705–2722.

    Jung KH, Han MJ, Lee DY, Lee YS, Schreiber L, Franke R, Faust A, Yephremov A, Saedler H, Kim YW, Hwang I, An G.2006. Wax-deficientis involved in cuticle and wax production in rice anther walls and is required for pollen development.,18(11):3015–3032.

    Lang NT, Subudhi PK, Virmani SS, Brar DS, Khush GS, Li Z, Huang N. 1999. Development of PCR-based markers for thermosensitive genetic male sterility gene(t) in rice (L.).,131(2):121–127.

    Lee DS, Chen LJ, Suh HS. 2005. Genetic characterization and fine mapping of a novel thermo-sensitive genic male-sterile genein rice (L.).,111(7):1271–1277.

    Li R B, Pandey MP, Sharma P, Ballabh G. 2005. Inheritance of thermosensitive genic male sterility in rice (L.).,88(11):1809–1815.

    Li N, Zhang DS, Liu HS, Yin CS,Li XX,Liang WQ,Yuan Z,Xu B,Chu HW,Wang J,Wen TQ,Huang H,Luo D,Ma H,Zhang DB. 2006. The rice tapetum degeneration retardation gene is required for tapetum degradation and anther development.,18(11):2999–3014.

    Liu N, Shan Y, Wang F P, Xu CG, Peng KM, Li XH, Zhang QF. 2001. Identification of an 85-kb DNA fragment containing, a locus for photoperiod-sensitive genic male sterility in rice.,266(2):271–275.

    Livak KJ, Schmittgen TD. 2001. Analysis of relative gene expression data using real-time quantitative PCR andthe 2 (-Delta Delta C(T)) method.,25(4):402–408.

    Lopez MT, Toojinda T, Vanavichit A, Tragoonrung S. 2003. Microsatellite markers flanking thegene facilitated tropical TGMS rice line development.,43(6):2267–2271.

    Matthayatthaworn W, Sripichitt P, Phumichai C, Rungmekarat S, Uckarach S, Sreewongchai T. 2011. Development of specific simple sequence repeat (SSR) markers for non-pollen type thermo-sensitive genic male sterile gene in rice (L.).,10:16437–16442.

    McCouch SR,Teytelman L, Xu Y, Lobos KB,Clare K,Walton M,Fu B,Maghirang R,Li Z,Xing Y,Zhang Q, Kono I,Yano M,Fjellstrom R,DeClerck G,Schneider D,Cartinhour S,Ware D,Stein L. 2002. Development and mapping of 2240 new SSR markers for rice (L.).,9(6):199–207.

    Murray MG, Thompson WF.1980. Rapid isolation of high molecular weight plant DNA.,8(19):4321–4325.

    Nas TMS, Sanchez DL, Diaz GQ, Mendioro MS, Vermani SS. 2005. Pyramiding of thermosensitive genetic male sterility(TGMS) genes and identification of a candidategene in rice.,145:67–75.

    Nonomura K I, Miyoshi K, Eiguchi M, Suzuki T, Miyao A, Hirochika H, Kurata N. 2003. Thegene is necessary to restrict the number of cells entering into male and female sporogenesis and to initiate anther wall formation in rice.,15(8):1728–1739.

    Peng HF, Zhang ZF, Wu B, Chen XH, Zhang GQ, Zhang ZM, Wan BH, Lu YP. 2008. Molecular mapping of two reverse photoperiod-sensitive genic male sterility genes (and) in rice (L.).,118(1):77–83.

    Peng HF, Chen XH, Lu YP, Peng YF,Wan BH,Chen ND,Wu B,Xin SP,Zhang GQ. 2010. Fine mapping of a gene for non-pollen type thermosensitive genic male sterility in rice (L.).,120(5):1013–1020.

    Pitnjam K, Chakhonkaen S, Toojinda T, Muangprom A. 2008. Identification of a deletion inand development of gene-based markers for selection.,228(5):813–822.

    Qi Y B, Liu Q L, Zhang L, Mao B Z, Yan D W, Jin Q S, He Z H. 2014. Fine mapping and candidate gene analysis of the novel thermo sensitive genic male sterility-gene in rice., 127(5): 1173–1182.

    Sharma M, Pandey GK. 2016. Expansion and function of repeat domain proteins during stress and development in plants.,6:1218.

    Sheng Z H, Wei X J, Shao G N, Chen M L, Song J, Tang S Q, Luo J, Hu Y C, Hu P S, Chen L Y. 2013. Genetic analysis and fine mapping of, a novel thermosensitive genic male-sterile gene in rice (L.).,132(2):159–164.

    Shin SB, Golovkin M, Reddy ASN. 2014. A pollen-specific calmodulin-binding protein, NPG1, interacts with putative pectate lyases.,4:5263.

    Small ID, Peeters N. 2000. The PPR motif:A TPR-related motif prevalent in plant organellar proteins.,25(2):45–47.

    Subudhi PK, Borkakati R P, Virmani SS, Huang N. 1997. Molecular mapping of a thermo-sensitive genetic male-sterility gene in rice using bulked segregant analysis.,40(2):188–194.

    Thai Rice Exporters Association. 2018. World Rice Production and Ending Stocks. http://www.thairiceexporters.or.th/default_eng.htm.

    USDA. 2016.World Agricultural Production. http://usda.mannlib.cornell.edu/usda/fas/worldag-production//2010s/2016/worldag-production-11-09-2016.pdf.

    Virmani SS. 2003. Advances in hybrid rice research and development in the tropics.: Virmani SS, Mao CX,Hardy B. Hybrid Rice for Food Security, Poverty Alleviation, and Environmental Protection. Proceedings of the 4th International Symposium on Hybrid Rice, Hanoi, Vietnam. Los Ba?os,the Philippines: International Rice Research Institute: 7–20.

    Wang B Y, Xu WW, Wang JZ, Wu W, Zheng HG, Yang ZY, Ray JD, Nguyen HT. 1995. Tagging and mapping the thermo-sensitive genic male-sterile gene in rice () with molecular markers.,91:1111–1114.

    Wang YG, Xing QH, Deng QY, Liang FS, Yuan LP, Weng ML, Wang B. 2003. Fine mapping of the rice thermo-sensitive genic male-sterile gene.,107(5):917–921.

    Xu J J, Wang B H, Wu Y H, Du P N, Wang J, Wang M, Yi C D, Gu M H, Liang G H. 2011. Fine mapping and candidate gene analysis of, the photoperiod-thermo-sensitive genic male sterile gene in rice (L.).,122(2):365–372.

    Yamaguchi Y, Ikeda R, Hirasawa H, Minami M, Ujihara A. 1997. Linkage analysis of thermo-sensitive genic male sterility genein rice (L.).,47:371–373.

    Yang QK, Liang CY, Zhuang W, Li J, Deng HB, Deng QY, Wang B. 2007. Characterization and identification of the candidate gene of rice thermo-sensitive genic male sterile geneby mapping.,225(2):321–330.

    Yang Y J, Ma C, Xu Y J, Wei Q, Imtiaz M, Lan H B, Gao S, Cheng L N, Wang M Y, Fei Z J, Hong B, Gao J P. 2014. A zinc finger protein regulates flowering time and abiotic stress tolerance in Chrysanthemum by modulating gibberellin biosynthesis.,26(5):2038–2054.

    Yang J, Chen X R, Zhu C L, Peng X S, He X P, Fu J R, Ouyang L J, Bian J M, Hu L F, Sun X T, Xu J, He H H. 2015. RNA-Seq reveals differentially expressed genes of rice () spikelet in response to interacting with nitrogen at meiosis stage.,16:959.

    Yuan LP. 1998. Hybrid rice breeding in China.: Virmani SS, Siddiq EA, Muralidharan K. Advances in Hybrid Rice Technology. Proceedings of the 3rd International Symposium on Hybrid Rice. Hyderabad, India. Manila, the Philippines: International Rice Research Institute: 27–33.

    Zhang Q F, Shen BZ, Dai XK, Mei MH, Saghai Maroof MA, Li ZB. 1994. Using bulked extremes and recessive classes to map genes for photoperiod-sensitive genic male sterility in rice.,91(18):8675–8679.

    Zhang Q S, Li Z, Yang J, Li S Q, Yang D C, Zhu Y G. 2012. A calmodulin-binding protein from rice is essential to pollen development.,55(1):8–14.

    Zhou YF, Zhang XY, Xue QZ. 2011. Fine mapping and candidate gene prediction of the photoperiod and thermo-sensitive genic male sterile gene(t)in rice.,12(6):436–447.

    Zhou H, Liu Q J, Li J, Jiang D G, Zhou L Y, Wu P, Lu S, Li F, Zhu L Y, Liu Z L, Chen L T, Liu Y G, Zhuang C X. 2012. Photoperiod- and thermo-sensitive genic male sterility in rice are caused by a point mutation in a novel noncoding RNA that produces a small RNA., 22(4):649–660.

    Zhou H, Zhou M, Yang Y Z,Li J, Zhu L Y, Jiang D G, Dong J F, Liu Q J, Gu L F, Zhou L Y, Feng M J, Qin P, Hu X C, Song C L, Shi J F, Song X W, Ni E D, Wu X J, Deng Q Y, Liu Z L, Chen M S, Liu Y G, Cao X F, Zhuang C X. 2014. RNase ZS1processes UbL40 mRNAs and controls thermosensitive genic male sterility in rice.,5:4884.

    Copyright ? 2019, China National Rice Research Institute. Hosting by Elsevier B V

    This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)

    Peer review under responsibility of China National Rice Research Institute

    http://dx.doi.org/10.1016/j.rsci.2018.08.006

    9 July 2018;

    24 August 2018

    Amorntip Muangprom (Amorntip.mua@biotec.or.th)

    (Managing Editor: Wang Caihong)

    亚洲人成网站在线播放欧美日韩| 超色免费av| 国产不卡一卡二| 无遮挡黄片免费观看| 亚洲国产毛片av蜜桃av| 亚洲精品在线观看二区| 熟女少妇亚洲综合色aaa.| 国产黄色免费在线视频| 妹子高潮喷水视频| xxx96com| 国产高清videossex| √禁漫天堂资源中文www| 免费不卡黄色视频| 久久久久久亚洲精品国产蜜桃av| 老司机福利观看| 麻豆国产av国片精品| 久久久国产欧美日韩av| 国产精品久久久久成人av| 99国产精品99久久久久| 99国产精品99久久久久| av电影中文网址| 欧美国产精品va在线观看不卡| 老熟妇乱子伦视频在线观看| 欧美日韩精品网址| 国产亚洲精品久久久久久毛片| 亚洲中文字幕日韩| 午夜视频精品福利| 久久人人精品亚洲av| 涩涩av久久男人的天堂| 美女午夜性视频免费| 欧美黑人欧美精品刺激| 久久香蕉国产精品| 久久中文看片网| 精品乱码久久久久久99久播| 久久天堂一区二区三区四区| 神马国产精品三级电影在线观看 | 黄片播放在线免费| 中文字幕高清在线视频| 高清欧美精品videossex| 国产精品一区二区精品视频观看| 国产一区二区三区视频了| 少妇粗大呻吟视频| 在线视频色国产色| 亚洲精品久久成人aⅴ小说| 国产91精品成人一区二区三区| 国产三级黄色录像| 真人一进一出gif抽搐免费| 久久国产精品人妻蜜桃| 999精品在线视频| 色在线成人网| 99国产精品免费福利视频| 99精品欧美一区二区三区四区| 淫秽高清视频在线观看| 欧美在线一区亚洲| 一级片免费观看大全| 久久精品亚洲av国产电影网| 久久久久久久久久久久大奶| 国产伦人伦偷精品视频| a在线观看视频网站| 波多野结衣一区麻豆| 日本撒尿小便嘘嘘汇集6| 老司机午夜福利在线观看视频| 91大片在线观看| 国产亚洲精品综合一区在线观看 | 涩涩av久久男人的天堂| 久久亚洲精品不卡| 国产不卡一卡二| 啦啦啦免费观看视频1| 99精品欧美一区二区三区四区| 亚洲成av片中文字幕在线观看| 国产精品自产拍在线观看55亚洲| 国产成人精品无人区| 真人一进一出gif抽搐免费| 精品国产一区二区久久| 高潮久久久久久久久久久不卡| 亚洲午夜理论影院| 亚洲国产精品合色在线| 精品一区二区三区视频在线观看免费 | 欧美日韩亚洲综合一区二区三区_| 国产成人精品久久二区二区91| 脱女人内裤的视频| 亚洲专区中文字幕在线| 丝袜美腿诱惑在线| 在线播放国产精品三级| 身体一侧抽搐| 老汉色av国产亚洲站长工具| 精品国产一区二区久久| 激情在线观看视频在线高清| 午夜福利,免费看| a级毛片在线看网站| x7x7x7水蜜桃| 久久香蕉激情| 国产av又大| 日韩一卡2卡3卡4卡2021年| 久久久久久亚洲精品国产蜜桃av| 久久久久久久久免费视频了| 亚洲午夜理论影院| 在线十欧美十亚洲十日本专区| 少妇粗大呻吟视频| www.999成人在线观看| 一进一出抽搐动态| 又黄又爽又免费观看的视频| 亚洲精品一二三| 久久精品亚洲av国产电影网| 国产亚洲精品综合一区在线观看 | 精品久久蜜臀av无| 一区二区三区激情视频| 国产成人精品在线电影| www国产在线视频色| 色老头精品视频在线观看| 久热这里只有精品99| 超色免费av| 欧美最黄视频在线播放免费 | 亚洲国产看品久久| 日韩免费高清中文字幕av| www日本在线高清视频| 18禁观看日本| 国产精品免费一区二区三区在线| av有码第一页| 一区在线观看完整版| 黄色丝袜av网址大全| 香蕉久久夜色| 色哟哟哟哟哟哟| 男女床上黄色一级片免费看| 国产精品久久视频播放| 久久草成人影院| 亚洲国产精品合色在线| 老汉色∧v一级毛片| 国产高清激情床上av| 91成年电影在线观看| 制服诱惑二区| 成人18禁在线播放| 高清欧美精品videossex| 精品久久久久久,| 欧美激情高清一区二区三区| 亚洲一区二区三区欧美精品| 波多野结衣一区麻豆| av天堂久久9| 一边摸一边抽搐一进一出视频| 久久婷婷成人综合色麻豆| 国产精品久久久人人做人人爽| 琪琪午夜伦伦电影理论片6080| 这个男人来自地球电影免费观看| 别揉我奶头~嗯~啊~动态视频| e午夜精品久久久久久久| 夫妻午夜视频| 欧美成人免费av一区二区三区| 少妇的丰满在线观看| 人妻丰满熟妇av一区二区三区| 岛国在线观看网站| 天天躁夜夜躁狠狠躁躁| 国产成年人精品一区二区 | 久久精品亚洲熟妇少妇任你| 日韩精品青青久久久久久| 亚洲成人精品中文字幕电影 | 精品高清国产在线一区| 水蜜桃什么品种好| 国产精品久久久av美女十八| 久久精品影院6| 国产精品自产拍在线观看55亚洲| 18禁美女被吸乳视频| 一级作爱视频免费观看| 亚洲精品国产一区二区精华液| 亚洲av片天天在线观看| 欧美日韩亚洲高清精品| 国产精品国产av在线观看| 嫩草影院精品99| 久久性视频一级片| 精品久久久久久成人av| 女人爽到高潮嗷嗷叫在线视频| 色哟哟哟哟哟哟| 国产精品av久久久久免费| 免费av中文字幕在线| 麻豆久久精品国产亚洲av | 又黄又粗又硬又大视频| 老汉色av国产亚洲站长工具| 一级片'在线观看视频| 天堂俺去俺来也www色官网| 欧美日韩中文字幕国产精品一区二区三区 | 亚洲一码二码三码区别大吗| 日日摸夜夜添夜夜添小说| 一级a爱片免费观看的视频| 麻豆av在线久日| 国产精品野战在线观看 | 我的亚洲天堂| 国产又爽黄色视频| 两性夫妻黄色片| 午夜福利影视在线免费观看| 亚洲,欧美精品.| 三级毛片av免费| 老熟妇乱子伦视频在线观看| 天天添夜夜摸| 国产日韩一区二区三区精品不卡| 91成人精品电影| 久久久久九九精品影院| 国产精品乱码一区二三区的特点 | 波多野结衣一区麻豆| 国产免费男女视频| 美女高潮到喷水免费观看| 99精品在免费线老司机午夜| 欧美av亚洲av综合av国产av| 女警被强在线播放| 亚洲熟女毛片儿| 亚洲性夜色夜夜综合| 多毛熟女@视频| 三上悠亚av全集在线观看| 国产成人精品久久二区二区91| 怎么达到女性高潮| 精品国产美女av久久久久小说| 精品一区二区三区四区五区乱码| 一边摸一边做爽爽视频免费| 国产av精品麻豆| 国产日韩一区二区三区精品不卡| 啦啦啦免费观看视频1| 久久久国产成人免费| 又黄又爽又免费观看的视频| 国产三级黄色录像| 亚洲情色 制服丝袜| 亚洲美女黄片视频| 国产欧美日韩综合在线一区二区| 国产亚洲精品久久久久久毛片| 黑丝袜美女国产一区| 久久影院123| 97碰自拍视频| 性色av乱码一区二区三区2| 日本wwww免费看| 水蜜桃什么品种好| 亚洲久久久国产精品| 香蕉国产在线看| 亚洲国产精品合色在线| 国产精品av久久久久免费| 欧美久久黑人一区二区| 日韩精品免费视频一区二区三区| 亚洲一区高清亚洲精品| 午夜精品国产一区二区电影| 1024视频免费在线观看| 国产亚洲精品一区二区www| 99热只有精品国产| 国产精品 欧美亚洲| 97碰自拍视频| 午夜精品久久久久久毛片777| 国产精品一区二区精品视频观看| 国产伦人伦偷精品视频| 9191精品国产免费久久| 天堂√8在线中文| 亚洲男人的天堂狠狠| 天天躁夜夜躁狠狠躁躁| 波多野结衣一区麻豆| 精品福利观看| 日韩欧美免费精品| 久久国产亚洲av麻豆专区| 一区二区日韩欧美中文字幕| www国产在线视频色| 老熟妇仑乱视频hdxx| 视频在线观看一区二区三区| 欧美日韩av久久| 91成年电影在线观看| 9色porny在线观看| avwww免费| 老司机午夜十八禁免费视频| 一进一出抽搐gif免费好疼 | 女人高潮潮喷娇喘18禁视频| 免费在线观看黄色视频的| 国产亚洲av高清不卡| 天堂中文最新版在线下载| 丁香六月欧美| 亚洲精品美女久久久久99蜜臀| 人妻久久中文字幕网| www.自偷自拍.com| 在线观看免费午夜福利视频| 国产一区二区三区视频了| 免费人成视频x8x8入口观看| 国产成+人综合+亚洲专区| 久久久水蜜桃国产精品网| 久久久久精品国产欧美久久久| 久久久国产欧美日韩av| 操出白浆在线播放| 黄色 视频免费看| 中亚洲国语对白在线视频| 亚洲av片天天在线观看| 久久久久久久午夜电影 | 精品久久久久久久毛片微露脸| 国产日韩一区二区三区精品不卡| 他把我摸到了高潮在线观看| 久久久国产一区二区| 日本五十路高清| 麻豆国产av国片精品| 国产精品一区二区精品视频观看| 亚洲aⅴ乱码一区二区在线播放 | 99在线视频只有这里精品首页| 黄色成人免费大全| 交换朋友夫妻互换小说| 欧美激情久久久久久爽电影 | 夜夜夜夜夜久久久久| 欧美亚洲日本最大视频资源| 青草久久国产| 国产亚洲精品久久久久久毛片| 亚洲aⅴ乱码一区二区在线播放 | 久9热在线精品视频| 久热爱精品视频在线9| 性色av乱码一区二区三区2| 亚洲中文日韩欧美视频| 一二三四社区在线视频社区8| 夫妻午夜视频| 两个人免费观看高清视频| 在线av久久热| 欧美日本亚洲视频在线播放| 亚洲免费av在线视频| 黑人巨大精品欧美一区二区蜜桃| 一进一出抽搐动态| 久久人人97超碰香蕉20202| 91国产中文字幕| 国产精品98久久久久久宅男小说| 老鸭窝网址在线观看| 热99国产精品久久久久久7| 免费高清在线观看日韩| 韩国av一区二区三区四区| 在线天堂中文资源库| 黄片播放在线免费| 99国产精品免费福利视频| 天堂影院成人在线观看| 1024香蕉在线观看| 丰满迷人的少妇在线观看| 久热这里只有精品99| 精品日产1卡2卡| 久久国产乱子伦精品免费另类| 精品无人区乱码1区二区| 欧美日韩亚洲高清精品| 成年人黄色毛片网站| 亚洲三区欧美一区| 91国产中文字幕| 亚洲精品成人av观看孕妇| 久久久久久久久久久久大奶| 亚洲精品一卡2卡三卡4卡5卡| 满18在线观看网站| 色老头精品视频在线观看| 国产亚洲精品久久久久5区| 在线国产一区二区在线| 大陆偷拍与自拍| 午夜日韩欧美国产| 亚洲狠狠婷婷综合久久图片| 韩国av一区二区三区四区| 90打野战视频偷拍视频| 黑人操中国人逼视频| av有码第一页| 色播在线永久视频| 国产一区二区三区在线臀色熟女 | 亚洲一码二码三码区别大吗| 9热在线视频观看99| 天堂中文最新版在线下载| 国产成+人综合+亚洲专区| 亚洲九九香蕉| 国产精品乱码一区二三区的特点 | 人妻久久中文字幕网| 国产男靠女视频免费网站| 欧美乱妇无乱码| 欧美不卡视频在线免费观看 | 日本一区二区免费在线视频| 成人精品一区二区免费| 国产不卡一卡二| 成人手机av| 黄色成人免费大全| 涩涩av久久男人的天堂| 国产精品久久视频播放| 无人区码免费观看不卡| 国产精品免费视频内射| 午夜福利,免费看| 亚洲国产中文字幕在线视频| av天堂在线播放| 日韩高清综合在线| 国产精品久久久久成人av| 亚洲人成77777在线视频| 日韩欧美在线二视频| 久久香蕉精品热| 国内毛片毛片毛片毛片毛片| 一区二区三区国产精品乱码| 日本精品一区二区三区蜜桃| 午夜久久久在线观看| 国产又爽黄色视频| 久久久久久亚洲精品国产蜜桃av| 纯流量卡能插随身wifi吗| 在线观看午夜福利视频| a级毛片黄视频| 极品教师在线免费播放| 国产在线精品亚洲第一网站| 国产主播在线观看一区二区| 老司机福利观看| 午夜免费成人在线视频| 99精品久久久久人妻精品| 老司机亚洲免费影院| 午夜亚洲福利在线播放| 色综合婷婷激情| 亚洲男人天堂网一区| 级片在线观看| 国产野战对白在线观看| 午夜老司机福利片| 国产成+人综合+亚洲专区| 国产精品一区二区三区四区久久 | 老熟妇仑乱视频hdxx| 免费女性裸体啪啪无遮挡网站| 久久久久久久久免费视频了| 亚洲第一av免费看| 69精品国产乱码久久久| av免费在线观看网站| 国产免费现黄频在线看| √禁漫天堂资源中文www| 大型黄色视频在线免费观看| 91国产中文字幕| 亚洲国产精品合色在线| 国产成人精品在线电影| 高清在线国产一区| 18禁观看日本| 免费看十八禁软件| 欧美乱色亚洲激情| 久久久久久大精品| 亚洲欧美日韩高清在线视频| 国产不卡一卡二| 国产区一区二久久| 一进一出抽搐gif免费好疼 | 精品高清国产在线一区| 在线免费观看的www视频| 性少妇av在线| 麻豆久久精品国产亚洲av | 水蜜桃什么品种好| 男女之事视频高清在线观看| 久久精品国产综合久久久| 国产一区二区在线av高清观看| 国产片内射在线| 日本欧美视频一区| av片东京热男人的天堂| 日韩免费av在线播放| 嫁个100分男人电影在线观看| 中文字幕精品免费在线观看视频| 欧美老熟妇乱子伦牲交| √禁漫天堂资源中文www| 99国产极品粉嫩在线观看| 91国产中文字幕| 亚洲 欧美 日韩 在线 免费| 欧美激情高清一区二区三区| 精品卡一卡二卡四卡免费| 19禁男女啪啪无遮挡网站| 老司机福利观看| 亚洲国产精品999在线| 视频区图区小说| 欧美精品亚洲一区二区| 国产精品自产拍在线观看55亚洲| 欧洲精品卡2卡3卡4卡5卡区| avwww免费| 中出人妻视频一区二区| 欧美日本亚洲视频在线播放| 99在线视频只有这里精品首页| 999久久久精品免费观看国产| 这个男人来自地球电影免费观看| 久久国产亚洲av麻豆专区| 人人妻,人人澡人人爽秒播| 成年人黄色毛片网站| 在线观看66精品国产| 日韩av在线大香蕉| 搡老熟女国产l中国老女人| 国产一区二区激情短视频| 黄色丝袜av网址大全| 久久亚洲精品不卡| 成人三级做爰电影| 精品久久久久久,| 十分钟在线观看高清视频www| 久久欧美精品欧美久久欧美| 中文字幕人妻熟女乱码| 热re99久久精品国产66热6| 一边摸一边抽搐一进一小说| 亚洲自偷自拍图片 自拍| 国产精品日韩av在线免费观看 | 精品久久蜜臀av无| 神马国产精品三级电影在线观看 | 亚洲av成人一区二区三| 欧美黑人欧美精品刺激| 黑人欧美特级aaaaaa片| 人人妻人人爽人人添夜夜欢视频| 大码成人一级视频| 久久久久亚洲av毛片大全| 无人区码免费观看不卡| 999久久久国产精品视频| 欧美乱妇无乱码| 天堂俺去俺来也www色官网| a级毛片在线看网站| 曰老女人黄片| 免费观看人在逋| 麻豆一二三区av精品| 黄色视频不卡| 人妻丰满熟妇av一区二区三区| 亚洲,欧美精品.| 久久午夜综合久久蜜桃| 亚洲视频免费观看视频| 亚洲精品国产色婷婷电影| 国产免费av片在线观看野外av| 天堂影院成人在线观看| 国产野战对白在线观看| 两人在一起打扑克的视频| 村上凉子中文字幕在线| 首页视频小说图片口味搜索| 日本五十路高清| 性欧美人与动物交配| 久久国产乱子伦精品免费另类| 欧美黄色淫秽网站| 色在线成人网| 欧美黄色淫秽网站| 在线视频色国产色| 久久精品国产99精品国产亚洲性色 | 丝袜美腿诱惑在线| 99精品在免费线老司机午夜| 亚洲男人天堂网一区| 人人澡人人妻人| 中文字幕最新亚洲高清| 人人妻,人人澡人人爽秒播| 99久久精品国产亚洲精品| 欧美日韩黄片免| 日韩有码中文字幕| 好男人电影高清在线观看| 精品熟女少妇八av免费久了| 超碰97精品在线观看| 如日韩欧美国产精品一区二区三区| 亚洲人成网站在线播放欧美日韩| 亚洲国产欧美一区二区综合| 手机成人av网站| 成在线人永久免费视频| 50天的宝宝边吃奶边哭怎么回事| 午夜福利,免费看| 制服人妻中文乱码| 欧美黑人欧美精品刺激| √禁漫天堂资源中文www| 欧美在线黄色| 国产激情久久老熟女| 欧美黑人精品巨大| 国产成人一区二区三区免费视频网站| 黑人巨大精品欧美一区二区蜜桃| x7x7x7水蜜桃| 亚洲自拍偷在线| 免费在线观看亚洲国产| 最好的美女福利视频网| 老司机午夜十八禁免费视频| 亚洲 国产 在线| 99久久精品国产亚洲精品| 亚洲伊人色综图| tocl精华| 亚洲欧美日韩另类电影网站| 这个男人来自地球电影免费观看| 欧美久久黑人一区二区| 日韩成人在线观看一区二区三区| 亚洲成a人片在线一区二区| 日本五十路高清| 欧美人与性动交α欧美精品济南到| 精品少妇一区二区三区视频日本电影| 18禁美女被吸乳视频| 国产视频一区二区在线看| a级毛片黄视频| 免费人成视频x8x8入口观看| 欧美不卡视频在线免费观看 | 91av网站免费观看| 真人做人爱边吃奶动态| 欧美激情极品国产一区二区三区| 精品久久久久久久久久免费视频 | 一个人观看的视频www高清免费观看 | 淫妇啪啪啪对白视频| 久久中文看片网| 在线播放国产精品三级| a级片在线免费高清观看视频| www.精华液| 免费av毛片视频| 久久久久久久久免费视频了| 91麻豆精品激情在线观看国产 | 精品人妻1区二区| 亚洲人成电影免费在线| 嫩草影视91久久| 日韩有码中文字幕| 不卡一级毛片| 别揉我奶头~嗯~啊~动态视频| 日本免费一区二区三区高清不卡 | 国产极品粉嫩免费观看在线| 久久久久亚洲av毛片大全| 中文字幕色久视频| 午夜福利一区二区在线看| 亚洲欧美日韩高清在线视频| 淫秽高清视频在线观看| 日韩国内少妇激情av| 中国美女看黄片| 黄色 视频免费看| 老司机午夜十八禁免费视频| 日本欧美视频一区| 国产高清videossex| 99在线人妻在线中文字幕| 成人精品一区二区免费| 精品免费久久久久久久清纯| 国产精品98久久久久久宅男小说| 香蕉国产在线看| 婷婷精品国产亚洲av在线| av网站在线播放免费| 国产一卡二卡三卡精品| 亚洲 欧美一区二区三区| 亚洲性夜色夜夜综合| 女性被躁到高潮视频| 中国美女看黄片| 波多野结衣高清无吗| 国产在线精品亚洲第一网站| 日韩av在线大香蕉| 亚洲伊人色综图| 黄色怎么调成土黄色| 色精品久久人妻99蜜桃| 亚洲欧美日韩无卡精品| 91精品国产国语对白视频| 国产精华一区二区三区| 一a级毛片在线观看| 咕卡用的链子| 中文字幕人妻熟女乱码| 国产91精品成人一区二区三区| www日本在线高清视频| 精品一区二区三区四区五区乱码|