Drought sensitivity ofbeech on a shallow chalk soilin northeastern Germany – a comparative study

Marieke van der Maaten-Theunissen,Hanna Bümmerstede,Janette Iwanowski,Tobias Scharnweber, Martin Wilmking and Ernstvan der Maaten

Drought sensitivity ofbeech on a shallow chalk soilin northeastern Germany – a comparative study

Marieke van der Maaten-Theunissen*,Hanna Bümmerstede,Janette Iwanowski,Tobias Scharnweber, Martin Wilmking and Ernstvan der Maaten

Background:We compare the climate sensitivity of European beech(Fagus sylvatica L.)in two forest nature reserves in northeastern Germany.The one reserve,Schlossberg,is characterized by shallow chalk soils,whereas in the other reserve,Eldena,soils are deeper and more developed.Little is known about the drought sensitivity of beech on shallow chalk soils.

Climate-growth relationships,Dendroecology,Inter-tree variability,Temporalstability,Drought stress

Background

European beech(Fagus sylvatica L.)is a tree species that naturally dominates over large parts of Central Europe due to a high competitiveness and physiologicaltolerance (Ellenberg 1996).The species thrives over broad environmental gradients and on many different bedrock types (Leuschner et al.2006),but does not grow on extremely dry sites,on sites prone to flooding,on sites with high groundwater tables,or on stagnic soils(Ellenberg 1996). Next to being ecologically important,e.g.,for biodiversity(Moning and Müller 2009),beech is a major economic tree species in European silviculture(Ge?ler et al.2007).

The climate sensitivity of beech has been investigated in many tree-ring based studies throughout the species distribution area(e.g.Chen et al.2015;Friedrichs et al. 2009;García-Suárez et al.2009;Lebourgeois et al.2005; Scharnweber et al.2011;van der Maaten 2012).These studies identified drought,in the previous and(or) current year,as a major growth-limiting factor.Despite its drought sensitivity,the resilience of beech to drought seems high.Van der Werf et al.(2007),for example,observed that growth of beech ceased during the extreme dry year 2003,but that it quickly recovered thereafter.In a future warmer climate,drought impacts are likely to become more severe,and may be particularlypronounced in the core of the species range as suggested by Cavin and Jump(2016).In their recent network study on beech,highest sensitivity and low resistance to drought is namely reported in the core of the species range,while dry range edge populations were characterized by particularly high drought resistance.Similarly, recent climate warming and increasing drought have impacted growth of beech more heavily on mesic sites compared to better adapted stands under marginal conditions(Weber et al.2013).Although provenance may be a factor in explaining these differences as well,i.e.different provenances may exhibit different adaptive behavior(Bolte et al.2007),the aforementioned studies show an important influence oflocalsoilconditions on reaction and adaptation of beech to drought and emphasize the need for detailed evaluations of drought sensitivity in dependence of wideranging climatic and environmentalconditions.

In investigating climate-growth relationships,tree-ring based studies mostly analyzed site chronologies that contain a maximized climate signal obtained by averaging tree-ring series of individual trees into one chronology (Fritts 1976).Variability in growth responses of individual trees,however,is ignored,and only general insights on how tree populations respond to climate fluctuations can be obtained.More recently,dendroecological studies started to explore the full range of tree-growth responses within sites by using individual-tree based approaches(e.g. Carrer 2011;Galván et al.2014;Scharnweber et al.2013; Zang et al.2014).In a climate change context,such studies are particularly important as the responses of individual trees willto a large degree determine how forest ecosystems may be affected by anticipated changes in climate.

In this study,we investigate the climate sensitivity of beech on a site with a shallow chalk soil in northeastern Germany.The water availability at such sites is assumed to be limited,and little is known on how these conditions translate into a drought signal in the growth record of beech.We compare the climate sensitivity of beech from a site with a shallow chalk soil with the sensitivity from a“typical”site in the same region,which has a deeper,more developed soil and a high water holding capacity.We hypothesize to find distinctively higher drought sensitivity for beech from the shallow chalk soil site.In analyzing climate-growth relationships,we not only consider mean stand chronologies,but also use tree-growth series from individual trees in linear mixed-effect models to explore the inter-tree variability in climate responses.

Methods

Site description

Fig.1(a)Location ofthe study sites;the inset map shows the study area within Europe as wellas the approximate naturaldistribution ofbeech in green according to EUFORGEN(2016).Climate diagrams display(b)mean monthly courses oftemperature(in °C)and precipitation sums(mm) forsite Schlossberg(calculated over the period 1902–2009),and(c)annualtime series of mean annualtemperature(MAT)and annual precipitation sum(MAP).Climate data originate from ClimateEU(fordetails see section'Climate data’)

This study was conducted in two old-growth beech forests in northeastern Germany,named Schlossberg and Eldena(Fig.1a).Both forests are nature reserves that were unmanaged for over 50 years.The nature reserve Schlossberg(54.5307°N,13.6515°E,elevation:135 m)is located on Rugia Island and lies within the borders ofJasmund National Park,of which part is declared UNESCO World Heritage Site('Primeval Beech Forests of the Carpathians and the Ancient Beech Forests of Germany’).The forest nature reserve of Eldena (54.0788°N,13.4787°E,elevation:17 m)is located near the city of Greifswald,and,as Schlossberg,in close vicinity to the Baltic coast.

Both studied forests are situated in a Young Drift morainic landscape,but differ in their pedological characteristics.Typical for the Schlossberg site is a shallow chalk soil.Depending upon the soil depth,which can be highly variable at short distance(presence of chalky parent material ranging between a few centimeters and＞1 m),soils can be characterized as leptosols(rendzinas),cambisols and luvisols(reference soil groups after the World Reference Base for Soil Resources;IUSS Working Group WRB 2015).According to the Dunham classification for carbonate sedimentary rocks(Dunham 1962),the chalk at Schlossberg can be characterized as a soft,weakly cemented biomicritic mudstone to wackestone,and only occasionally as floatstone with over 10%＞2 mm grains(Schnick 2002).At Eldena the parent material is sandy-loamy glacial till.Further,soils are deeper and more developed(i.e.no presence of leptosols,but only cambisols and luvisols),and locally there is influence of stagnating water and groundwater.

The regional climate can be characterized as temperate humid with a mean annual air temperature and precipitation sum of 7.5 °C/576 mm and 8.1 °C/562 mm at Schlossberg and Eldena,respectively.A climate diagram characterizing the general climate at Schlossberg (Fig.1b)shows that precipitation sums are highest in summer.For Eldena,seasonal courses of temperature and precipitation are highly similar(see the difference plot of Additional file 1:Figure S1).In the second half of the twentieth century,increases in air temperature are evident,whereas no clear trends can be observed for precipitation(Fig.1c).

Tree-ring data

We selected co-/dominant beech trees at Schlossberg (n=25)and Eldena(n=12),and extracted two increment cores at breast height from these trees.After air-drying,cores were either mounted on wooden holders and sanded with progressively finer grit sandpaper to highlight annual rings,or prepared for scanning in an ITRAX multiscanner(Cox Analytical Systems,Sweden).In both cases,tree-ring widths(TRW)were measured optically, and TRW-series for individualtrees were obtained by averaging the measurements of the two cores.Visual and statistical crossdating of the tree-ring series was done using the CooRecorder/CDendro software package(Cybis Elektronik and Data AB,Sweden).

Ring-width series of individual trees were detrended by fitting a cubic smoothing spline with a 50%frequency cut-off at 30 years.This procedure accentuates climate-induced growth fluctuations while removing longer-term trends that may,for example,reflect tree ageing or disturbance(Cook and Peters 1981).Tree-ring indices were then calculated by dividing the observed by the predicted values.To characterize the developed index chronologies and to assess their quality,we report the common chronology statistics Gleichl?ufigkeit(glk), inter-series correlation(RBAR),mean sensitivity(MS), first-order autocorrelation(AC)and expressed population signal(EPS).Glk is the percentage of sign agreement in year-to-year ring-width changes,RBAR is the average correlation coefficient between all tree-ring series,MS is the average relative difference in tree growth between consecutive years,and AC is a measure of previous-year influence on current-year growth.EPS is a statistic used to assess the adequacy of the replication in a site chronology.We built chronologies and calculated chronology statistics using dplR(Bunn 2008).

Climate data

We obtained site-specific climate data using the software package ClimateEU(Hamann et al.2013;Wang et al. 2012;available for download at http://tinyurl.com/Cli mateEU).This software front-end for interpolated climate databases,generated with the Parameter-elevation Regressions on Independent Slopes Model(PRISM) (Daly et al.2008),allows to query 84 historical monthly, seasonal and annual climate variables for the years 1901-2009.An overview of all queried variables is provided as supplementary material(Additionalfile 1:Table S1).

Statisticalanalyses

We calculated bootstrapped correlation coefficients between site chronologies and climate data to identify climatic factors responsible for the observed growth variation.In these correlation analyses,we included monthly temperature(minimum,mean and maximum) and precipitation over a 16-month window from June of the previous year to September of the current year, as well as previous-and current-year seasonal and annual climate variables.Climate-growth relationships were established over the common period 1902-2009 using treeclim(Zang and Biondi 2015).

As the aforementioned climate-growth correlation analyses disregard inter-tree variability in climate responses(i.e.these standard analyses are based on average tree-growth indices of all sampled trees within a stand),we also employed linear mixed-effect models.By fitting these models on individual tree-growth indices with tree as random factor and climate variables as fixed effects,we could account for possible differences inclimate response of single trees.The model can be formulated as follows:

where RWIirepresents the growth index for tree i,α the intercept,Climithe fixed-effects matrix(i.e.climate variables), β and bivectors of fixed and random effects,and εia vector with random errors.We used the restricted maximum likelihood(REML)to determine the optimal random effects structure of our models(i.e.random intercept,random slope,or both random intercept and random slope),and maximum likelihood(ML)estimation to compare models with different fixed effects.We considered all climate variables from the climate-growth correlation analyses as fixed effects,but standardized them prior to model development(with a mean of 0 and a standard deviation of 1)to allow for direct comparison of modelcoefficients.

Linear mixed-effect models were built sequentially with up to three climate parameters.First,we tested all one-parameter models and compared their performance using the Akaike information criterion(AIC;Akaike 1974)corrected for small sample sizes(AICc).AIC penalizes complex models with more parameters;smaller values indicate a more parsimonious model.We used the difference in AICcbetween the nullmodeland allcandidate models(ΔAICc)to indicate the extent to which candidate models explain the variation in tree growth.In a second step,we added a second climate variable to the three best-performing one-parameter models.Similarly,a third climate variable was added to the three bestperforming two-parameter models.In adding a second and third climate variable,we excluded models with highly correlated explanatory variables(criterion:r ≥ 0.5;cf. Ettinger et al.(2011)who used a 0.6-threshold).Next to model coefficients and ΔAICcvalues,we report the marginal R2(the variance explained by the fixed factors;calculated after Nakagawa and Schielzeth (2013)for all candidate models.In building and testing our linear mixed-effect models,we used the R packages nlme(Pinheiro et al.2014)and piecewiseSEM(Lefcheck 2016).

To evaluate if climatic drivers of beech growth changed through time,we fitted linear mixed-effect models not only over the full climate data period(1902–2009), but also over an early and late sub-period(1902–1955 and 1956–2009,respectively).

Results

Metadata and chronology statistics for the study sites are provided in Table 1,site chronologies in Fig.2.Remarkable is a higher tree age at Schlossberg(indicated by the mean segment length:236 versus 200 years),but lower mean ring width.Chronology statistics for thedeveloped index chronologies,however,are highly similar. High values for glk and RBAR indicate that trees show similar growth patterns within the individual sites.Also between sites similarities in growth patterns are evident; index chronologies are strongly correlated(r=0.72;period 1902–2009).MS values of 0.33 point to high year-to-year variability in growth.Finally,EPS-values beyond the commonly used threshold of 0.85 indicate a coherent standlevelsignal.

Table 1 Characteristics ofthe study trees

The climate-growth correlation analyses indicate that previous-year summer temperature is a significant negative determinant of beech growth at both sites(Fig.3). At the same time,previous-year summer precipitation positively affects beech growth,pointing to possible drought stress.Correlations with annual climate variables substantiate the importance of previous-year drought for current-year’s growth(Additional file 1: Figure S2).Also for the current year,a drought signal is suggested by positive correlations with spring and June precipitation as well as negative correlations with mean June temperature.Although there are some differences in correlation strengths for individual climate parameters,general correlation patterns are remarkably similar for Schlossberg and Eldena.

At the individual tree level,random intercept linear mixed-effect models revealed previous-year drought conditions as an important growth-limiting factor as well.At the Schlossberg site,this is illustrated by negative effects of previous-year July maximum temperature (pTmax07)and positive effects of previous-year August precipitation(pPPT08)in numerous best-fit models (Table 2).For Eldena,the previous-year climate moisturedeficit(pCMD)was found as a major growth determinant(Table 3).For the one-parameter models,climate response patterns are consistent with the results of the correlation analysis(Additional file 1:Figures S3–S6).

Fig.2(a)Raw tree-ring width and(b)detrended index chronologies for Schlossberg(black)and Eldena(gray)

Fig.3 Results of the bootstrapped correlation analysis between tree-growth indices and monthly and seasonalclimate data for(a)Schlossberg and(b)Eldena.Bars indicate correlations forprecipitation,lines fortemperature(dotted line:mimimum temperature;solid line:average temperature; dashed line:maximum temperature).Significant correlations(P ＜ 0.05)are indicated by dark gray bars(for precipitation)and filled circles(for temperature).Months and seasons are abbreviated with lower-and uppercase letters for the previous-and current-year growing season, respectively.WT winter(December(previous year)-February),SP spring(March-May),SM summer(June-August),AT autumn (September-November)

Table 2 Statisticalparameters of the three best-performing linear mixed-effect models of tree-ring indices with one,two or three climate variables for site Schlossberg

In the two sub-periods 1902–1955 and 1956–2009, drought signals are evident as well,but are expressed through different climate predictors.In the early period, current-year climate parameters prevail(Schlossberg: PPT_sp;Eldena:Tmax06 and PPT_sp),whereas carryover effects of drought seem less important(i.e.expressed by relatively few previous-year drought parameters).In the late period,on the other hand,previous-year climate parameters prevail with previous July and summer(maximum)temperature as the most frequent climate determinants(pTmax07/pTave07 and pTmax_sm)at Schlossberg and Eldena,respectively.

The explained variance of all best-fit one-to threeparameter models ranged between 4.9 and 22.1%,with marginal R2-values being consistently higher for the early and late sub-period compared to the full period.Further,the explained variance for Schlossberg is higher than for Eldena,which implies that drought parameters better explain the observed growth variation,suggesting a(slightly)higher drought sensitivity of beech at Schlossberg.Overall,the inter-tree variability in climatic responses was low with variance components for the random tree effect approaching zero.

Table 3 Statisticalparameters of the best-performing linear mixed-effect models for site Eldena.See further the table legend and footnotes of Table 2

Discussion

Individual trees showed strong common growth signals at our research sites as evidenced by high values for glk,RBAR and EPS(Table 1),as well as by a low intertree variability observed in our climate-driven linear mixed-effect models of tree-growth indices.These results underline that the investigated trees are affected by a common climatic factor,i.e.drought,and that our sampling of co-/dominant trees resulted in a uniform dataset without much inter-tree variability.On the contrary,studies that not only sampled dominant trees but also intermediate and(or)suppressed trees(e.g.Cescatti and Piutti 1998;Martín-Benito et al.2008)report intertree variability.Larger beech trees(like we sampled in our study)were found to be more sensitive to summer drought than smaller trees(e.g.Mérian and Lebourgeois2011),which likely relates to higher evaporative water losses that large trees face because of their large crowns (St?elcováet al.2002).

Both in the classical climate-growth correlation analyses(Fig.3 and Additional file 1:Figure S2)as well as in our linear mixed-effect models(Tables 2 and 3, Additional file 1:Figures S3–S6)drought signals prevailed at Schlossberg and Eldena.Although climate response patterns were highly similar,a higher explained variance of the models for Schlossberg suggests that this site is slightly more drought sensitive.As a possible explanation for the lower-than-expected drought sensitivity of beech at Schlossberg,we hypothesize that adaptations in tree architecture safeguarded the beech trees from severe drought problems at the site (Kahle 1994;van der Maaten et al.2013).This hypothesis is supported by our metadata showing that,although trees at Schlossberg are older than at Eldena, tree height and DBH are lower(Table 1),which likely results in a lower demand for water.As an alternative explanation,we propose that the water holding capacity of the shallow chalk soils might be higher than assumed.Although the available water capacity of the upper soil is lower at Schlossberg(i.e.soils are shallow),the localchalk bedrock shows a fine texture with relatively high silt and clay contents and is fullof fissures.This probably contributes to relatively good water storage capacities of the bedrock,which is plant available if the trees are able to penetrate the parent material with their roots.That shallow chalk soils can supply enough water for plant growth is also suggested by a study on the productivity of agricultural crops in England,which reports that even the shallowest chalk soils(23 cm)provide enough water to sustain good yields(Burnham and Mutter 1993).

We found drought signals in our models for Schlossberg and Eldena for both the full climate data period (1902–2009),as well as for the early and late sub-periods(1902–1955 and 1956–2009,respectively).Although climate response patterns remained relatively stable over time,a shift in importance from current to previous-year drought influences was observed from the early to the late sub-period.Next to possible effects of ageing,this shift might relate to a co-occurring temperature increase in the second half of the twentieth century(Fig.1c).Namely, higher temperatures may increase evaporative water losses,thereby making last years’water status more important.Next to direct effects of the previous-year water budget on the current-year growth,previous-year drought effects may also be explained over floral induction(Di Filippo et al.2007).Various studies showed that hot and dry summer conditions stimulate masting in beech in the following year(Piovesan and Adams 2001;Schmidt 2006), thereby negatively affecting growth(Dittmar et al.2003; Drobyshev et al.2010).In line with these results,Hacket-Pain et al.(2015)showed that regression models explained growth of beech equally well when replacing previousyear maximum temperature by current-year seed production.Although there is no data available on beech masting at Schlossberg and Eldena,a general observation of more frequent and intense fructification in Germany over recent decades(Paar et al.2011)suggests that masting may also be a likely cause for the shift from current-to previousyear drought parameters in our models.

In contrast to classical dendroecological studies that aim for strong climatic signals by averaging individual tree-growth series into a site chronology(Fritts 1976), we employed an additional linear mixed-effect modeling exercise.Rather than filtering-out tree-specific signals by averaging over multiple trees,which is a process that might be beneficial for climate reconstructions,linear mixed-effect models use the growth series of all trees while estimating a model true for the whole population. The models provide insight into the variability in growth responses ofindividualtrees to climate,thereby providing essentialinformation on the range of tree species responses to climate,and consequently on how they may respond to future climate changes.Even though inter-tree variability was low in our tree-ring dataset(i.e.variance components for the random tree effect were approaching zero),we support Carrer(2011)in advocating an increased focus on climate responses of individual trees in the future.From recent individual tree-based studies,we learned,amongst others, that growth sensitivity may increase with age(Linares et al. 2013),or that the variability in individual-tree responses during and after drought events may be high(Zang et al.2014). Such insights are of upmost importance in the face of climate change,as it willnot be the averaged responses of forest stands,but the growth responses of individual trees that willdetermine how forests may be affected in the future.

Conclusion

We compared the climate sensitivity of European beech on a shallow chalk soil in northeastern Germany with beech trees on a site with deeper and more developed soils,and found that beech is only slightly more drought sensitive on the shallow chalk soil.

Additional file

Additional file 1:Supplementary figures and table.(PDF 193 kb)

Acknowledgments

We thank the Jasmund NationalPark and the forest service ofthe University of Greifswald forsupporting ourfieldwork.

Funding

MMT and EM acknowledge support by a research grant of the Eva Mayr-Stihl Foundation.MMT and MW are participating researchers of the research training group RESPONSE,funded by the German Research Council(DFG GRK2010).

Authors’contributions

Conceived and designed the study:MMT and EM.Performed the field-and lab-work:MMT,HB,JI,TS,EM.Analyzed the data:MMT and EM.Wrote the manuscript:MMT,TS,MW,EM.Allauthors read and approved the final

manuscript.

Author’s information

MMT,EM and TS are postdoctoralforestecologists mainly working in

European forests.They have a specialinterest in understanding the role of plasticity and adaptation of tree species in assessing potentialimpacts of

climate change.Professor MW is a geo-ecologistworking on the ecosystem dynamics oftrees and shrubs in temperate and borealregions,as wellas the arctic tundra.HB and JIare graduate studentsthatwrote their Bachelortheses on the ecology ofbeech in Jasmund nationalpark.Allauthors are working at (or associated to)the Institute of Botany and Landscape Ecology atthe

University of Greifswald,Germany.

Competing interests

The authors declare that they have no competing interests.

Field permits

Research permissions to sample European beech trees at Schlossberg and Eldena were provided by the Jasmund National Park and the lower nature conservation authority Vorpommern-Greifswald,respectively.

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*Correspondence:marieke.theunissen@uni-greifswald.de;

marieketheunissen@gmail.com

Institute of Botany and Landscape Ecology,University of Greifswald, Soldmannstr.15,17487 Greifswald,Germany

Methods:We collected increment cores at both research sites and established climate-growth relationships.Intertree variability was assessed by employing linear mixed-effect models.

Results:We expected to find distinctively higherdrought sensitivity at Schlossberg due to limited wateravailability,but find only marginaldifferences in growth responses.Atboth sites,drought is the major climatic factordriving tree growth. Adaptations in tree architecture and an underestimation ofthe waterholding capacity ofshallow chalk soils are discussed as possible reasons for not finding more distinctclimate responses.In analyzing climate-growth relationships,we specifically focused on growth responses ofindividualtrees but observed only low inter-tree variability at both sites. Evidentis a shift in climate response patterns from the first to the second halfofthe twentieth century with previous-year droughtconditions becoming more important than current-year drought.This shift is discussed in relation to a warming trend overthatsame period,as wellas possible trends in masting behaviorofbeech.

Conclusion:The investigated beech trees on the shallow chalk soilare only slightly more drought sensitive than beech trees on the reference site with deeperand more developed soils.