• <tr id="yyy80"></tr>
  • <sup id="yyy80"></sup>
  • <tfoot id="yyy80"><noscript id="yyy80"></noscript></tfoot>
  • 99热精品在线国产_美女午夜性视频免费_国产精品国产高清国产av_av欧美777_自拍偷自拍亚洲精品老妇_亚洲熟女精品中文字幕_www日本黄色视频网_国产精品野战在线观看 ?

    Concurrent infections of dengue viruses serotype 2 and 3 in patient with severe dengue from Jakarta, Indonesia

    2016-11-29 12:06:04SoroyLardoYaldieraUtamiBenediktusYohanSeriMMUTariganWidayatDjokoSantosoLeonardNainggolanTedjoSasmono

    Soroy Lardo, Yaldiera Utami, Benediktus Yohan, Seri MMU Tarigan, Widayat Djoko Santoso, Leonard Nainggolan, R Tedjo Sasmono*

    1Division of Tropical Medicine and Infectious Diseases, Department of Internal Medicine, Gatot Soebroto Central Army Hospital, Jakarta, Indonesia

    2Department of Internal Medicine, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia

    3Division of Tropical Medicine and Infectious Diseases, Department of Internal Medicine, Cipto Mangunkusumo National Central Hospital, Jakarta, Indonesia

    4Eijkman Institute for Molecular Biology, Jakarta, Indonesia

    Concurrent infections of dengue viruses serotype 2 and 3 in patient with severe dengue from Jakarta, Indonesia

    Soroy Lardo1, Yaldiera Utami2, Benediktus Yohan4, Seri MMU Tarigan2, Widayat Djoko Santoso3, Leonard Nainggolan3, R Tedjo Sasmono4*

    1Division of Tropical Medicine and Infectious Diseases, Department of Internal Medicine, Gatot Soebroto Central Army Hospital, Jakarta, Indonesia

    2Department of Internal Medicine, Faculty of Medicine, University of Indonesia, Jakarta, Indonesia

    3Division of Tropical Medicine and Infectious Diseases, Department of Internal Medicine, Cipto Mangunkusumo National Central Hospital, Jakarta, Indonesia

    4Eijkman Institute for Molecular Biology, Jakarta, Indonesia

    ARTICLE INFO

    Article history:

    Received 15 November 2015

    Received in revised form 20 December 2015

    Accepted 15 January 2016

    Available online 20 February 2016

    Severe dengue

    Concurrent infections

    DENV-2

    DENV-3

    Objective: To describe the clinical manifestation of patient with severe dengue, to identify the serotypes and genotypes of dengue viruses (DENV) which concurrently infecting the patient, and to explore the possible relationship of severe dengue with the concurrent infection of DENV. Methods: Dengue diagnosis was performed using NS1 antigen detection and IgG/ IgM ELISA. Standard clinical and laboratory examinations were performed to obtain the clinical and hematological data. DENV concurrent infections were detected and confirmed using RT-PCR and DENV Envelope gene sequencing. Phylogenetic analyses were performed to determine the genotypes of the viruses. Results: The patient was classified as having severe dengue characterized by severe plasma leakage, hemorrhage, and organ damage involving lung, liver, and kidney. Concurrent infection of DENV serotype 2 and 3 was observed. The infecting DENV-2 virus was grouped into Cosmopolitan genotype while DENV-3 virus was classified into Genotype Ⅰ. Both viruses were closely related to isolates that were endemic in Jakarta. Viremia measurement was conducted and revealed a significantly higher virus titer of DENV-3 compared to DENV-2. Conclusions: The occurrence of multi-serotype DENV infections was presented in a patient with severe clinical manifestation in Indonesia. The hyperendemicity of dengue in Indonesia may contribute to the DENV concurrent infections cases and may underlie the severity of the disease.

    1. Introduction

    Dengue is currently one of the most important arboviral disease in the world[1]. The disease is caused by infection of dengue virus (DENV), which is transmitted to human through the bites of Aedes spp. mosquito vectors. DENV consisted of four antigenically distinct but genetically similar serotypes, which are designated as DENV-1, DENV-2, DENV-3 and DENV-4[2]. Dengue clinical manifestations vary from asymptomatic or mild flu-like syndrome known as classicdengue fever to more severe form known as dengue hemorrhagic fever (DHF) and the potentially fatal dengue shock syndrome[3].

    Dengue regularly occurred in tropical and sub-tropical regions around the world[4]. Co-circulation of multiple DENV serotypes is considered as one of the factors contributing to high cases of dengue infection. The circulation of multiple DENV serotypes in the same area has been reported since decades ago in countries in Southeast Asia, Central America, and South America[1]. In 2011, the entire tropical world is hyperendemic with multiple virus serotypes cocirculating in most large urban centers[1]. Indonesia is a tropical country that is hyperendemic to dengue, in which all four DENV serotypes circulating in the region. Dengue has become major public health problem in the country since it was first discovered in 1968 in Surabaya[5] and currently, the disease occurred in all 34provinces in Indonesia[6]. Dengue cases occurred throughout the year and outbreaks occurred periodically such as those in 1998[7] and 2004[8].

    Concurrent infections by more than one DENV serotypes have been suggested to influence clinical manifestation, and this was considered as one of the explanations for the emergence of DHF[9]. Living in dengue hyperendemic region, people in Indonesia have high probabilities of having concurrent DENV infections which may lead to severe dengue. Concurrent infections of DENV in Indonesia was first documented during 1975-1978 epidemics[10]. And then, more recent cases were also reported[11]. In this study, we reported a concurrent DENV-2 and DENV-3 infection case in patient with severe dengue. We examined the clinical, diagnostic, virological and molecular aspects of this DENV concurrent infections case.

    2. Materials and methods

    2.1. Case report, sample collection and serological tests

    A 38-years old female patient (ID JKT-AD001) was administered to Gatot Soebroto Central Army Hospital in Jakarta, Indonesia on 16 April 2013. Serum sample was collected at day five of fever and routine diagnosis for confirmation of dengue infection was performed. Written informed consent was obtained from the patient. Serology and DENV NS1 antigen detection were performed using the Panbio NS1 rapid test and IgG/IgM ELISA (Alere, Brisbane, Australia). The IgG/IgM ELISA results were used to determine primary versus secondary dengue infection, according to manufacturer’s protocol. Briefly, primary dengue infection was indicated by positive IgM (>11 Panbio Units) and negative IgG (<22 Panbio Units) while secondary dengue infection was indicated by positive IgG (>22 Panbio Units), which may be accompanied by elevated IgM levels.

    2.2. RNA extraction, DENV detection and serotyping

    Strict controls were applied on RNA extraction and PCR preparation/reaction procedures to prevent cross-contamination between samples. All of activities were performed in separate areas/ containments and using separate sets of equipment conducted within Good Clinical Laboratory Practice-certified laboratory at the Eijkman Institute. DENV genomic RNA was extracted from 140 μL of patient’s serum using QIAamp Viral RNA Mini kit (Qiagen, Hilden, Germany). DENV nucleic acid detection and serotyping were performed using two steps conventional RT-PCR according to protocol described by Lanciotti et al[12], with modification according to Harris et al[13]. Superscript Ⅲ RT enzyme (Invitrogen-Life Technologies, Carlsbad, CA) was used in the reverse-transcription reaction. The resulting cDNA was used in subsequent PCR reactions using Taq DNA Polymerase (Roche, Mannheim, Germany).

    2.3. Determination of viral load

    Two steps quantitative RT-PCR (qRT-PCR) analysis was used to quantify the virus titer in serum according to protocol adapted from conventional RT-PCR method by Lanciotti et al[12]. Extracted RNA was reverse-transcribed using D2 antisense primer. The resulting cDNA was used as template in qRT-PCR reaction performed using Power SYBR Green PCR kit and 7500 RT-PCR System (Applied Biosystems-Life Technologies, Foster City, CA). Viral load determination was performed in separate reaction mixes using serotype-specific primers D1 and TS2 and D1 and TS3 for DENV-2 and DENV-3, respectively. The reaction was prepared in triplicate using 400 nM of primers final concentration and thermal cycling setup of 10 min initial denaturation step at 95 ℃, followed by 35 cycles of 10 s denaturation step at 95 ℃ and 1 min annealing/ extension step at 60 ℃. An additional dissociation stage was used to verify the melting temperature of amplicons. A recombinant plasmid standard was generated by cloning DENV genome fragment covering the C, prM/M, and Envelope (E) genes into PCRBlunt plasmid using Zero Blunt PCR Cloning Kit (Invitrogen-Life Technologies) according to the manufacturer’s instructions. Known copy number of recombinant plasmid was serially diluted and used as genome copy number standards in RT-PCR analyses. The copy number standards were then used to quantify genome copy number of viral RNA transcripts from 10-fold dilutions of known DENV titer measured by plaque assay in BHK21 cells. The resulting copy number-titer equivalent standard curve was used to calculate viral load in sample determined as DENV plaque forming unit (PFU) equivalent/mL of serum.

    2.4. DENV Envelope gene sequencing

    Viral RNA was extracted directly from serum sample and used as template for DENV genotyping using E gene sequences according to protocol described previously[11]. Capillary sequencing reactions were performed to obtain complete E gene sequences of DENV-2 (1 485 nt) and DENV-3 (1 479 nt), employing serotype-specific primers described elsewhere[14]. In order to prevent possible contamination from DENV reference strains/positive controls being used, we have performed E gene sequencing for all of the reference strains available in our laboratory and maintained a controlled virus archiving procedure. The E gene sequences obtained in this study have been deposited in GenBank with accession numbers KJ184316 (for isolate JKT-AD001A) and KJ184317 (for isolate JKT-AD001B).

    2.5. DENV genotype analyses

    DENV genotype analyses were performed to generate classification of isolate sequences into genotypes. Isolate sequences were combined with the downloaded GenBank sequences according to sample’s serotypes to create dataset for each genotypes grouping based on Twiddy[15] and Lanciotti[16] classifications for DENV-2, and DENV-3, respectively. Multiple sequence alignment for E gene was performed using MUSCLE[17]. Dataset for each serotype was prepared using BEAUti v.1.7.5[18] and followed by phylogenetic reconstruction analysis using Bayesian Markov chain Monte Carlo method as implemented in BEAST v 1.7.5[19] using GTR+Γ4model with codon model, relaxed uncorrelated lognormal molecular clock and Bayesian skyline prior, with 100 million generations and sampled for every 1 000th iteration. All parameters showedeffective sampling size values of >100, measured using Tracer v.1.5.0. Maximum clade credibility (MCC) tree was created using TreeAnnotator v.1.7.5 and visualized in FigTree v.1.4.0, which are available inside the BEAST package.

    3. Results

    3.1. Clinical manifestation

    A 38-year old female patient was administered to Gatot Soebroto Central Army Hospital in Jakarta, Indonesia after experiencing four days fever accompanied with myalgia, arthralgia, headache, vomiting, skin rash, epistaxis, and gum bleeding. Initial physical examination on the first day of hospitalization observed a normotensive blood pressure with 38.6 ℃ fever. Patient was fully conscious but presented a shortness of breath and bleeding gums. Lung examination presented smooth wet rhonchi, whereas the abdominal examination showed epigastric tenderness but no hepatomegaly observed. Petechiae were observed in both arms. Chest X-ray showed dextral pleural effusion. Normal electrocardiography was observed. Patient was diagnosed as DHF Grade Ⅲ based on high fever with headache, myalgia, arthralgia, vomiting, abdominal pain, appetite loss, epistaxis and bleeding gums, wet rhonchi, mid-epigastric pain, petheciae and evidence of plasma leakage which include pleural effusion and hypoproteinemia. Thrombocytopenia and elevated level of transaminase enzymes were observed, however, hematocrit was still within normal level during acute phase. The patient condition on day five of hospitalization was worsened and then moved to ICU.

    Further development in ICU, the patient showed stable vital signs and better breathiness. No fever, headache, abdominal pain, epistaxis and bleeding gums were observed. Appetite was gradually increased and nausea was absence. Vaginal bleeding was decreasing. At the tenth day of treatment in the ICU, a stable vital sign was observed and the patient was free of fever for approximately 3 d. Shortness of breath and hemorrhagic manifestations were not observed, although physical examination observed extensive hematoma in the arm region around the venipuncture area.

    3.2. Clinical laboratory findings

    Laboratory examination observed severe thrombocytopenia (13 000/μL) during febrile period, while final platelet count of 156 000/μL was observed when the patient has been fully recovered (convalescence phase at d 15 after hospitalization) (Table 1). Although thrombocytopenia was prominent in this patient, no significant hematocrit increment was observed during the course of hospitalization, although slight increase was observed during convalescence. The transaminase enzymes levels, markers for the liver function, showed gradual increment with the maximum level for SGOT/SGPT reached 5 641/1 807 IU/L. When the patient was fully recovered, the final levels of 82/134 IU/L were observed (Table 1). Leukocytosis was observed, with a maximum count of 22 300/μL. Leukocytosis was then improved to 8 000/μL at convalescence period (Table 1). We also examined the electrolyte level in the patient, in which hyponatremia, hypocalcemia, and hypoalbuminemia were observed (Table 1). Improvements of the above hematological findings were observed when patient in convalescence period. A sterile blood culture results was obtained. Chest X-ray result consistent with a sign of pneumonia. Patient was fully recovered.

    Table 1Clinical laboratory findings of patient with concurrent DENV infections.

    3.3. Disease complication

    We observed disease complications that may have effect to the clinical findings. Pulmonologist consultant observed the pulmonary edema with bronchopneumonia, and hemoptysis. The patient has a history of tuberculosis, and secondary bacterial infection was proposed based on fever, asphyxia, and coughing with white phlegm. Obstetrician diagnosed the patient with abnormal uterus bleeding caused by endometrium hyperplasia that had occurred within the last two months. This was confirmed by USG examination.

    3.4. DENV serology, RT-PCR, and viral load determination

    Patient serum collected on day five of fever was tested positive by anti-dengue NS1 Rapid Test. The anti-dengue IgM and IgG ELISA were also performed on the serum and both were positive (data not shown). The infection status of the patient was secondary infection as determined by the IgM and IgG values, performed according to the assay method. Confirmation of DENV infection was further pursued using RT-PCR to detect and serotype the infecting virus. The RT-PCR detected the presence of two PCR amplicons which were corresponded to DENV-2 and DENV-3, albeit of fainter band for DENV-2 (Figure 1A). To quantitatively determine the titers of the infecting viruses, we performed qRT-PCR detection of viremia in the patient’s serum. The presence of concurrent infections was further confirmed using this method (Figure 1B), and the DENV-2 virus titer was determined as 2.5×102PFU equivalent/mL of serum, while DENV-3 viral load reached 6.8×104PFU equivalent/mL (Figure 1B).

    Figure 1. (A) Detection of DENV concurrent infections in JKT-AD001 patient’s serum using conventional RT-PCR.

    3.5. DENV E gene sequencing and phylogenetic analysis

    Given the presence of two DENV serotypes in RT-PCR detection and serotyping result, we sought to further confirm the concurrent DENV infections by using E gene sequencing. Amplification of E gene in RNA directly extracted from serum sample successfully obtained both DENV-2 and DENV-3 E gene amplicons (data not shown). The E gene fragments were then subjected to DNA sequencing. The sequences of the E genes confirmed the serotypes of the infecting viruses as DENV-2 and DENV-3. To determine the genotype of the viruses, we performed phylogenetic analyses of both DENVs. Our phylogenetic analysis of DENV-2 grouped the infecting virus into Cosmopolitan genotype according to Twiddy et al[15] (Figure 2). The isolate (JKT-AD001A) was closely related to viruses we recently isolated in Sukabumi, West Java province, Indonesia in 2012[20] and grouped together with isolates from other cities in Indonesia such as Jakarta isolated in 1994 and 2004, Semarang (isolated in 2012[11]), and Palembang (isolated in 1998) (Figure 2). Meanwhile, phylogenetic analysis of DENV-3 E protein gene revealed the genotype of the infecting virus as Genotype Ⅰbased on Lanciotti classification[16].

    Figure 2. MCC tree of DENV-2 genotypes grouping generated by Bayesian inference method as implemented in BEAST using GTR evolution model and gamma parameter rates from the E gene sequences.

    As seen in Figure 3, the virus isolate (JKT-AD001B) was closely related with viruses from Jakarta (isolated in 2004), Palembang (isolated in 1998), Bandung (isolated in 1998), and Semarang (isolated in 2012).

    Figure 3. MCC tree of DENV-3 genotypes grouping generated by Bayesian inference method as implemented in BEAST using GTR evolution model and gamma parameter rates from the E gene sequences.

    4. Discussion

    Dengue has become a health problem in many tropical countries including Indonesia, in which hyperendemicity contributes to the increasing dengue cases. Coupled with high vector index, the occurrence of concurrent infections with multiple DENV serotypes is highly possible. With the advancement of DENV detection technologies, more and more concurrent dengue infections cases were reported. Concurrent infections cases have been reported in Mexico, Puerto Rico, Taiwan, China, Brazil, Thailand, and India[10,21-28]. For Indonesia, the concurrent infections were first reported during outbreaks in 1976-1978, which was accounted for 11.1% of the cases[10]. We have also recently reported other concurrent infections cases in Semarang city, Indonesia[11] and other cities in Indonesia[29]. In this study, we reported a concurrent infections case in patient from Jakarta, a city with highest dengue incidence rate in Indonesia[6]. We believe that concurrent infections cases may also occurred in other cities in Indonesia. However, because of the limited data on DENV surveillance in Indonesia, the available data may be under-reported. This study was the first to report the concurrent infections case in Indonesia in detail, in which the clinical, diagnostic, molecular and virological aspects of the infection were presented.

    Concurrent infections have been proposed as one of contributing factors to severe dengue[9]. However, several reports described the absence of relationship between concurrent infections with severe disease[10,26]. Unlike those reports, the patient reported in this study presented a severe dengue. The patient exhibited abdominal pain and persistent vomiting, clinical warning signs of severe dengue[4]. Leukocytosis was also observed, which may indicate a warning sign of severe dengue[30]. The atypical manifestations of dengue are uncommon, but there have been increasing reports of DF and DHF with unusual manifestation[4,31]. In this patient, atypical manifestations of dengue were observed and involved the hepatic, pulmonary, and renal manifestations. It has been reported that DHF may cause mild to moderate liver dysfunction in most cases, however, only some patients may suffer from acute liver failure[32]. The hepatic manifestation in this patient was characterized by increasing level of aminotransferase enzymes. The pulmonary involvement included shortness of breath, pleural effusion, cough, and hemoptysis. Although renal failure is a rare complication in dengue infections, the deranged serum urea and creatinine levels during the course of illness in this patient were indicative of renal dysfunction. Altogether, these are consistent with severe dengue.

    As mentioned above, although reports indicated that concurrent infections did not related with severe dengue[10,26], other studies observed the higher percentage of cases with concurrent infections had severe disease[25,27]. Thus, our study is in accordance with those reports.

    Previous study has demonstrated that higher viremia titer was associated with more severe disease[33]. In this report, we measured the viremia using qRT-PCR. To our knowledge, our study is the first to present data on the viral loads of concurrent DENV infections. We observed a significantly higher titer of DENV-3 (ie. 272 times) compared to the DENV-2. This data suggest that, compared to DENV-2, the DENV-3 might possessed better replication rate in the patient and might predominantly replicated in the body and thus affect the immune system. However, we are not sure whether the higher viral loads of DENV-3 compared to DENV-2 was occurred since the early days of infection since we only measured the viral load in a single time point (day five of fever). As far as we aware, there is no previous study that describe the comparative fitness of DENV in patient with concurrent infections. Further, it may be that the severity of disease in concurrent infections is not caused by the synergistic action of both serotypes, but more likely caused by one of the virus that have higher virus titer, just as in single infection cases. As this study only observed the difference in serotype-specific viral load in one patient, we cannot confirm that the finding is generalizable to other concurrent infections cases. Determination of viral loads in larger number of concurrent infections cases will be beneficial to confirm this observation.

    With respect to the infecting DENV, we determined the genotypes of both DENV-2 and DENV-3 viruses using phylogenetic analyses based on E protein gene sequences. The DENV-2 isolate was grouped into Cosmopolitan genotype according to Twiddy et al[15]. This genotype is widely distributed in many countries such as in India, countries in South East Asia, Africa, the Middle East, and Australia[15]. In term of genetic relationship with other DENV-2 isolates, this isolate was closely related and grouped together with DENV-2 viruses from Jakarta isolated in 2004, and from other cities in Indonesia such as Sukabumi (isolated in 2012), and Palembang (isolated in 1998). This suggested that the infecting virus was endemic in the area, and has been circulated for a long time. Similarly, the genotype of infecting DENV-3, which was grouped into Genotype Ⅰ according to Lanciotti et al[16], was closely related to DENV-3 viruses from Jakarta and other cities in Indonesia such as Bandung, Palembang, and Semarang. Altogether, our phylogenetic data demonstrated the sustainable circulation of endemic strains of DENV in Indonesia which regularly infecting people and actively transmitted in the community.

    In summary, our study presented the occurrence of multi-serotype DENV infections in a patient with severe clinical manifestation in Indonesia. The hyperendemicity of dengue in Indonesia may contribute to the DENV concurrent infections cases and may underlie the severity of the disease.

    Conflict of interest statement

    We declare that we have no conflict of interest.

    Acknowledgements

    The authors would like to thank patient involved in this study. Eijkman Institute is under the auspices of the Ministry of Research, Technology, and Higher Education of the Republic of Indonesia, and the authors would like to thank the Ministry for providing financial support for this study.

    [1] Gubler DJ. Dengue, urbanization and globalization: the unholy trinity of the 21st century. Trop Med Health 2011; 39(4): 3-11.

    [2] Simmons CP, Farrar JJ, van Nguyen VC, Wills B. Dengue. N Engl J Med 2012; 366(10): 1423-1432.

    [3] Martina BEE, Koraka P, Osterhaus ADME. Dengue virus pathogenesis: an integrated view. Clin Microbiol Rev 2009; 22(10): 564-581.

    [4] WHO. Comprehensive guidelines for prevention and control of dengue and dengue haemorrhagic fever. World Health Organization, 2011.

    [5] Sumarmo. Dengue haemorrhagic fever in Indonesia, Southeast Asian. J Trop Med Public Health 1987; 18(3): 269-274.

    [6] Ministry of Health Republic of Indonesia. Indonesia Health Profile 2010. Jakarta; 2011.

    [7] Corwin AL, Larasati RP, Bangs MJ, Wuryadi S, Arjoso S, Sukri N, et al. Epidemic dengue transmission in southern Sumatra, Indonesia. Trans R Soc Trop Med Hyg 2001; 95(3): 257-265.

    [8] Suwandono A, Kosasih H, Nurhayati, Kusriastuti R, Harun S, Ma’roef C, et al. Four dengue virus serotypes found circulating during an outbreak of dengue fever and dengue haemorrhagic fever in Jakarta, Indonesia, during 2004. Trans R Soc Trop Med Hyg 2006; 100(9): 855-862.

    [9] Hammon WM. Dengue hemorrhagic fever-do we know its cause? Am J Trop Med Hyg1973; 22(1): 82-91.

    [10] Loro?o-Pino MA, Cropp CB, Farfán JA, Vorndam AV, Rodríguez-Angulo EM, Rosado-Paredes EP, et al. Common occurrence of concurrent infections by multiple dengue virus serotypes. Am J Trop Med Hyg 1999; 61(5): 725-730.

    [11] Fahri S, Yohan B, Trimarsanto H, Sayono S, Hadisaputro S, Dharmana E, et al. Molecular surveillance of dengue in semarang, indonesia revealed the circulation of an old genotype of dengue virus serotype-1. PLoS Negl Trop Dis 2013; 7(8): e2354.

    [12] Lanciotti RS, Calisher CH, Gubler DJ, Chang GJ, Vorndam AV. Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase-polymerase chain reaction. J Clin Microbiol 1992; 30(3): 545-551.

    [13] Harris E, Roberts TG, Smith L, Selle J, Kramer LD, Valle S, et al. Typing of dengue viruses in clinical specimens and mosquitoes by single-tube multiplex reverse transcriptase PCR. J Clin Microbiol 1998; 36(9): 2634-2639.

    [14] Ong SH, Yip JT, Chen YL, Liu W, Harun S, Lystiyaningsih E, et al. Periodic re-emergence of endemic strains with strong epidemic potential-a proposed explanation for the 2004 Indonesian dengue epidemic. Infect Genet Evol 2008; 8(2): 191-204.

    [15] Twiddy SS, Farrar JJ, Vinh Chau N, Wills B, Gould EA, Gritsun T, et al. Phylogenetic relationships and differential selection pressures among genotypes of dengue-2 virus. Virology 2002; 298(1): 63-72.

    [16] Lanciotti RS, Lewis JG, Gubler DJ, Trent DW. Molecular evolution and epidemiology of dengue-3 viruses. J Gen Virol 1994; 75(Pt1): 65-75.

    [17] Edgar RC. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 2004; 32(5): 1792-1797.

    [18] Drummond AJ, Suchard MA, Xie D, Rambaut A. Bayesian phylogenetics with BEAUti and the BEAST 1.7. Mol Biol Evol 2012; 29(8): 1969-1973.

    [19] Drummond AJ, Rambaut A. BEAST: Bayesian evolutionary analysis by sampling trees. BMC Evol Biol 2007; 7: 214.

    [20] Nusa R, Prasetyowati H, Meutiawati F, Yohan B, Trimarsanto H, Setianingsih TY, et al. Molecular surveillance of dengue in Sukabumi, west Java province, Indonesia. J Infect Dev Ctries 2014; 8(6): 733-741.

    [21] Laille M, Deubel V, Sainte-Marie FF. Demonstration of concurrent dengue 1 and dengue 3 infection in six patients by the polymerase chain reaction. J Med Virol 1991; 34(1): 51-54.

    [22] de Carvalho Araújo FM, Nogueira RMR, de Araújo JMG, Ramalho ILC, de Sá Roriz MLF, de Melo MEL, et al. Concurrent infection with dengue virus type-2 and DENV-3 in a patient from Ceará, Brazil. Mem Inst Oswaldo Cruz 2006; 101(8): 925-928.

    [23] Chinnawirotpisan P, Mammen Jr MP, Nisalak A, Thaisomboonsuk B, Narupiti S, Thirawuth V, et al. Detection of concurrent infection with multiple dengue virus serotypes in Thai children by ELISA and nested RT-PCR assay. Arch Virol 2008; 153(12): 2225-2232.

    [24] Wenming P, Man Y, Baochang F, Yongqiang D, Tao J, Hongyuan D, et al. Simultaneous infection with dengue 2 and 3 viruses in a Chinese patient return from Sri Lanka. J Clin Virol 2005; 32: 194-198.

    [25] Bharaj P, Chahar HS, Pandey A, Diddi K, Dar L, Guleria R, et al. Concurrent infections by all four dengue virus serotypes during an outbreak of dengue in 2006 in Delhi, India. Virol J 2008; 5(1). Doi:10.1186/1743-422X-5-1.

    [26] Gubler DJ, Kuno G, Sather GE, Waterman SH. A case of natural concurrent human infection with two dengue viruses. Am J Trop Med Hyg 1985; 34(1): 170-173.

    [27] Vinodkumar CS, Kalapannavar NK, Basavarajappa KG, Sanjay D, Gowli C, Nadig NG, et al. Episode of coexisting infections with multiple dengue virus serotypes in central Karnataka, India. J Infect Public Health 2013; 6(4): 302-306.

    [28] Wang WK, Chao DY, Lin SR, King CC, Chang SC. Concurrent infections by two dengue virus serotypes among dengue patients in Taiwan. J Microbiol Immunol Infect 2003; 36(2): 89-95.

    [29] Aryati, Trimarsanto H, Yohan B, Wardhani P, Fahri S, Sasmono RT. Performance of commercial dengue NS1 ELISA and molecular analysis of NS1 gene of dengue viruses obtained during surveillance in Indonesia. BMC Infect Dis 2013; 13: 611.

    [30] Lee IK, Liu JW, Yang KD. Fatal dengue hemorrhagic fever in adults: emphasizing the evolutionary pre-fatal clinical and laboratory manifestations. PLoS Negl Trop Dis 2012; 6(2): e1532.

    [31] Gulati S, Maheshwari A. Atypical manifestations of dengue. Trop Med Int Health TM IH 2007; 12(9): 1087-1095.

    [32] Nguyen TL, Nguyen TH, Tieu NT. The impact of dengue haemorrhagic fever on liver function. Res Virol 1997; 148(4): 273-277.

    [33] Vaughn DW, Green S, Kalayanarooj S, Innis BL, Nimmannitya S, Suntayakorn S, et al. Dengue viremia titer, antibody response pattern, and virus serotype correlate with disease severity. J Infect Dis 2000; 18(1): 2-9.

    ent heading

    10.1016/j.apjtm.2016.01.013

    *Corresponding author: R Tedjo Sasmono, Eijkman Institute for Molecular Biology, Jl. Diponegoro 69, Jakarta 10430, Indonesia.

    Tel: +62-21-3917131

    Fax: +62-21-3147982

    E-mail: sasmono@eijkman.go.id

    永久网站在线| 午夜老司机福利剧场| 精品一区二区三区av网在线观看| 美女大奶头视频| 女的被弄到高潮叫床怎么办 | 久久久精品大字幕| 99热这里只有是精品50| 最新在线观看一区二区三区| 中国美女看黄片| 中文字幕熟女人妻在线| 成人国产麻豆网| 亚洲成人免费电影在线观看| 亚洲av中文av极速乱 | 一级黄片播放器| 国产高清有码在线观看视频| 亚洲av免费在线观看| 日本黄色视频三级网站网址| av.在线天堂| 在线观看66精品国产| 久久香蕉精品热| eeuss影院久久| 88av欧美| 亚州av有码| 99热只有精品国产| 久久久久久久久大av| 97人妻精品一区二区三区麻豆| 91av网一区二区| 精品人妻视频免费看| 97超视频在线观看视频| 亚洲aⅴ乱码一区二区在线播放| 精品久久国产蜜桃| 精品一区二区免费观看| 美女免费视频网站| 亚洲乱码一区二区免费版| 亚州av有码| 国产精品久久视频播放| 国产av一区在线观看免费| 欧美人与善性xxx| 身体一侧抽搐| 欧美日韩综合久久久久久 | 免费在线观看影片大全网站| 亚洲精品在线观看二区| 国国产精品蜜臀av免费| 色视频www国产| 午夜免费男女啪啪视频观看 | 国产精品国产高清国产av| 午夜精品一区二区三区免费看| 国产美女午夜福利| 91麻豆av在线| 伊人久久精品亚洲午夜| 最新中文字幕久久久久| 一区二区三区免费毛片| 国产在线精品亚洲第一网站| 91久久精品电影网| 国产 一区精品| 欧美又色又爽又黄视频| 亚洲电影在线观看av| 一个人看的www免费观看视频| 一区二区三区高清视频在线| 精品久久国产蜜桃| 日本黄色片子视频| 看免费成人av毛片| 精品一区二区免费观看| 校园人妻丝袜中文字幕| 国产精品爽爽va在线观看网站| 久久精品国产亚洲av香蕉五月| 人人妻人人澡欧美一区二区| 亚洲成人精品中文字幕电影| 国产精品女同一区二区软件 | 自拍偷自拍亚洲精品老妇| 色哟哟·www| 欧美黑人巨大hd| 亚洲欧美日韩高清专用| 色精品久久人妻99蜜桃| 91久久精品国产一区二区成人| 综合色av麻豆| 搡老妇女老女人老熟妇| 亚洲avbb在线观看| 国产一级毛片七仙女欲春2| 亚洲av免费在线观看| 国内精品宾馆在线| 久久国产精品人妻蜜桃| 99热这里只有精品一区| 日本-黄色视频高清免费观看| 搡老熟女国产l中国老女人| 成年女人看的毛片在线观看| 国产精品日韩av在线免费观看| 嫁个100分男人电影在线观看| 亚洲va在线va天堂va国产| 欧美性感艳星| 成年女人永久免费观看视频| 国产精品日韩av在线免费观看| 能在线免费观看的黄片| 亚洲成av人片在线播放无| 欧美又色又爽又黄视频| 亚洲黑人精品在线| 日韩大尺度精品在线看网址| 成人av一区二区三区在线看| 日韩欧美 国产精品| 欧美日韩黄片免| 欧美日韩黄片免| 国产精品98久久久久久宅男小说| 午夜免费成人在线视频| 嫩草影院入口| 免费观看精品视频网站| 亚洲精品在线观看二区| 亚洲精品影视一区二区三区av| 国产av一区在线观看免费| 久久久久国产精品人妻aⅴ院| 午夜日韩欧美国产| 精品国内亚洲2022精品成人| 最近最新中文字幕大全电影3| 色综合色国产| 免费电影在线观看免费观看| av女优亚洲男人天堂| 少妇的逼水好多| 婷婷精品国产亚洲av在线| 亚洲av美国av| 一进一出好大好爽视频| 国产女主播在线喷水免费视频网站 | 女人十人毛片免费观看3o分钟| 特大巨黑吊av在线直播| 全区人妻精品视频| 亚洲av成人av| 午夜亚洲福利在线播放| 日韩强制内射视频| 国产精品日韩av在线免费观看| 亚洲性夜色夜夜综合| 在线看三级毛片| 久久久久久久久久成人| 国产精品久久久久久精品电影| 在线观看午夜福利视频| 免费观看精品视频网站| 人人妻人人澡欧美一区二区| 久久这里只有精品中国| 搡女人真爽免费视频火全软件 | 欧美成人免费av一区二区三区| 国产免费男女视频| 国产私拍福利视频在线观看| 两人在一起打扑克的视频| 国产探花极品一区二区| 免费电影在线观看免费观看| 简卡轻食公司| 色尼玛亚洲综合影院| 亚洲人成网站在线播| 久久久久久久久久久丰满 | 精品国产三级普通话版| 亚洲最大成人手机在线| 熟妇人妻久久中文字幕3abv| 美女黄网站色视频| 麻豆精品久久久久久蜜桃| 欧美日韩瑟瑟在线播放| 日本色播在线视频| 色噜噜av男人的天堂激情| 国产老妇女一区| 成人高潮视频无遮挡免费网站| 一区二区三区高清视频在线| 精品免费久久久久久久清纯| 亚洲天堂国产精品一区在线| 亚洲欧美日韩无卡精品| 精品欧美国产一区二区三| 欧美日本视频| 国产精品久久久久久精品电影| 亚洲av一区综合| 中亚洲国语对白在线视频| 很黄的视频免费| 久久草成人影院| 日韩大尺度精品在线看网址| 午夜a级毛片| 桃红色精品国产亚洲av| 午夜视频国产福利| 黄片wwwwww| 日本一二三区视频观看| 亚洲中文日韩欧美视频| 夜夜看夜夜爽夜夜摸| 久久久午夜欧美精品| 久久久成人免费电影| 狂野欧美激情性xxxx在线观看| 99热这里只有是精品在线观看| 国产精品一区二区三区四区免费观看 | 精品不卡国产一区二区三区| 看十八女毛片水多多多| 女人十人毛片免费观看3o分钟| or卡值多少钱| 日本一二三区视频观看| 中文字幕免费在线视频6| 国内精品一区二区在线观看| 日本与韩国留学比较| 十八禁网站免费在线| 狠狠狠狠99中文字幕| 最新在线观看一区二区三区| 国产精品野战在线观看| 成人永久免费在线观看视频| 国产一级毛片七仙女欲春2| 一级a爱片免费观看的视频| 黄色女人牲交| 欧美日本亚洲视频在线播放| 日韩强制内射视频| 中文在线观看免费www的网站| 此物有八面人人有两片| 亚洲精品国产成人久久av| 久久久久九九精品影院| 久久精品国产99精品国产亚洲性色| 国内精品久久久久精免费| 三级男女做爰猛烈吃奶摸视频| 哪里可以看免费的av片| 成熟少妇高潮喷水视频| 亚洲精品亚洲一区二区| 亚洲精品影视一区二区三区av| 99久久精品一区二区三区| 3wmmmm亚洲av在线观看| 99热6这里只有精品| 黄色配什么色好看| 国产精品久久久久久亚洲av鲁大| 精品午夜福利在线看| 搡老妇女老女人老熟妇| 欧美中文日本在线观看视频| 亚洲成人久久性| 久久6这里有精品| 成人综合一区亚洲| 香蕉av资源在线| 在线免费观看不下载黄p国产 | 中文字幕熟女人妻在线| 久久精品国产亚洲av天美| 伦理电影大哥的女人| 久久人人精品亚洲av| 男女边吃奶边做爰视频| 男人的好看免费观看在线视频| 看片在线看免费视频| 深夜a级毛片| 男人和女人高潮做爰伦理| 国产伦在线观看视频一区| 亚洲av美国av| 亚洲第一电影网av| 97人妻精品一区二区三区麻豆| 一区二区三区免费毛片| av黄色大香蕉| 欧美+日韩+精品| 亚洲第一区二区三区不卡| 国产精品,欧美在线| 午夜激情欧美在线| 亚洲成人久久性| 久久亚洲真实| 中文字幕av在线有码专区| 精品一区二区免费观看| 校园春色视频在线观看| 日韩欧美精品v在线| 一本一本综合久久| 日韩在线高清观看一区二区三区 | 亚洲av美国av| 亚洲精华国产精华精| 国产精品永久免费网站| 日本与韩国留学比较| 亚洲欧美日韩高清专用| 亚洲成人免费电影在线观看| 丝袜美腿在线中文| 搡老熟女国产l中国老女人| 天堂动漫精品| 久久国产精品人妻蜜桃| 久久久成人免费电影| 日本一二三区视频观看| 校园春色视频在线观看| 成人精品一区二区免费| 亚洲电影在线观看av| 亚洲精品粉嫩美女一区| 精品人妻一区二区三区麻豆 | 色综合亚洲欧美另类图片| 欧美3d第一页| 午夜福利在线在线| 午夜精品久久久久久毛片777| 国产乱人伦免费视频| 国产高清视频在线观看网站| 18禁在线播放成人免费| 99国产精品一区二区蜜桃av| 亚洲av免费高清在线观看| 国产av一区在线观看免费| 网址你懂的国产日韩在线| 亚洲熟妇中文字幕五十中出| 男女那种视频在线观看| 男女做爰动态图高潮gif福利片| 日韩亚洲欧美综合| 变态另类成人亚洲欧美熟女| av在线天堂中文字幕| 欧美3d第一页| 日韩一区二区视频免费看| 日本黄色视频三级网站网址| 免费av毛片视频| 欧美色视频一区免费| 亚洲,欧美,日韩| 亚洲熟妇熟女久久| 天堂网av新在线| 男女下面进入的视频免费午夜| 中文字幕精品亚洲无线码一区| 国产亚洲精品久久久久久毛片| 一区二区三区四区激情视频 | 最近中文字幕高清免费大全6 | 18+在线观看网站| www日本黄色视频网| 国产成人av教育| 国产极品精品免费视频能看的| 成人午夜高清在线视频| 伦精品一区二区三区| АⅤ资源中文在线天堂| 精品久久久久久久久久免费视频| 国产精品久久久久久精品电影| 亚洲欧美精品综合久久99| 亚洲专区国产一区二区| 深夜a级毛片| 亚洲欧美日韩卡通动漫| 亚洲国产精品久久男人天堂| 日本免费一区二区三区高清不卡| 国产淫片久久久久久久久| 欧美区成人在线视频| 99久久无色码亚洲精品果冻| 免费无遮挡裸体视频| 日本免费a在线| 日韩在线高清观看一区二区三区 | 午夜福利成人在线免费观看| ponron亚洲| 两个人视频免费观看高清| 成年女人毛片免费观看观看9| 黄色配什么色好看| 亚洲av免费在线观看| 成人鲁丝片一二三区免费| 男女啪啪激烈高潮av片| 国产欧美日韩一区二区精品| 三级国产精品欧美在线观看| 欧美色视频一区免费| 日本黄色视频三级网站网址| 男人的好看免费观看在线视频| 黄色日韩在线| 日本 欧美在线| 亚洲无线观看免费| 丰满人妻一区二区三区视频av| 禁无遮挡网站| 亚洲三级黄色毛片| 国产精品综合久久久久久久免费| 国产精品三级大全| 久久国产精品人妻蜜桃| 亚洲自偷自拍三级| 啦啦啦观看免费观看视频高清| 国产私拍福利视频在线观看| 老女人水多毛片| 一进一出好大好爽视频| 久久人妻av系列| 高清在线国产一区| 国产精品国产高清国产av| 国产精品一及| 一级黄片播放器| 久久热精品热| 在线免费观看不下载黄p国产 | 久久久国产成人精品二区| av天堂在线播放| 精品无人区乱码1区二区| 国产乱人视频| 国产精品一区www在线观看 | 在线免费观看不下载黄p国产 | 琪琪午夜伦伦电影理论片6080| 国内精品久久久久久久电影| 老司机深夜福利视频在线观看| 亚洲美女视频黄频| 精品一区二区三区视频在线| 欧美三级亚洲精品| 国产欧美日韩一区二区精品| 成人毛片a级毛片在线播放| 国产亚洲精品久久久com| 九九在线视频观看精品| 99久久精品国产国产毛片| 三级男女做爰猛烈吃奶摸视频| videossex国产| 亚洲va日本ⅴa欧美va伊人久久| 免费不卡的大黄色大毛片视频在线观看 | 欧美日韩瑟瑟在线播放| 观看美女的网站| 国产探花极品一区二区| 欧美在线一区亚洲| 色综合色国产| 成人无遮挡网站| 又紧又爽又黄一区二区| 精品久久久久久久末码| 一区二区三区激情视频| 免费人成视频x8x8入口观看| 天堂网av新在线| 大型黄色视频在线免费观看| 舔av片在线| 九九爱精品视频在线观看| 婷婷精品国产亚洲av在线| 少妇人妻精品综合一区二区 | 午夜精品一区二区三区免费看| 丰满人妻一区二区三区视频av| 精品人妻一区二区三区麻豆 | 亚洲av美国av| 最新在线观看一区二区三区| 亚洲最大成人中文| 久久这里只有精品中国| 99精品久久久久人妻精品| 老熟妇乱子伦视频在线观看| 91在线精品国自产拍蜜月| 不卡视频在线观看欧美| 免费人成视频x8x8入口观看| 精品不卡国产一区二区三区| 性色avwww在线观看| 欧美区成人在线视频| 性色avwww在线观看| 动漫黄色视频在线观看| 别揉我奶头~嗯~啊~动态视频| 亚洲欧美日韩东京热| 免费电影在线观看免费观看| 狂野欧美白嫩少妇大欣赏| 亚洲欧美日韩东京热| 久久久久久大精品| 国产在线精品亚洲第一网站| 99在线人妻在线中文字幕| 啦啦啦韩国在线观看视频| 亚洲熟妇中文字幕五十中出| 国产伦精品一区二区三区四那| 国产精品爽爽va在线观看网站| 欧美高清成人免费视频www| 舔av片在线| 天天一区二区日本电影三级| 午夜福利在线观看吧| av在线亚洲专区| 国产av不卡久久| 极品教师在线视频| 国产极品精品免费视频能看的| 免费大片18禁| 18+在线观看网站| 欧美性猛交╳xxx乱大交人| 国产伦在线观看视频一区| 国产伦人伦偷精品视频| 日本成人三级电影网站| 成年人黄色毛片网站| 91午夜精品亚洲一区二区三区 | 18禁黄网站禁片免费观看直播| 如何舔出高潮| 色综合亚洲欧美另类图片| 精品日产1卡2卡| 91久久精品电影网| 我要搜黄色片| 老师上课跳d突然被开到最大视频| 欧美又色又爽又黄视频| 成人无遮挡网站| 黄色日韩在线| 国内精品宾馆在线| 又粗又爽又猛毛片免费看| 日本撒尿小便嘘嘘汇集6| 久久人人精品亚洲av| 成人亚洲精品av一区二区| 国产v大片淫在线免费观看| 国产乱人伦免费视频| 亚洲熟妇熟女久久| 女的被弄到高潮叫床怎么办 | 色视频www国产| 深夜a级毛片| 久久精品国产鲁丝片午夜精品 | 搡老熟女国产l中国老女人| 欧美zozozo另类| 国产熟女欧美一区二区| av女优亚洲男人天堂| 亚洲真实伦在线观看| 中出人妻视频一区二区| 美女黄网站色视频| 久久国产精品人妻蜜桃| 国产一区二区在线观看日韩| av女优亚洲男人天堂| 亚洲av五月六月丁香网| 色综合站精品国产| 天堂影院成人在线观看| 日韩欧美一区二区三区在线观看| 日韩大尺度精品在线看网址| 国内毛片毛片毛片毛片毛片| 变态另类成人亚洲欧美熟女| 国产精品一区www在线观看 | av黄色大香蕉| 国产一区二区在线av高清观看| 亚洲午夜理论影院| 少妇猛男粗大的猛烈进出视频 | 亚州av有码| 日本撒尿小便嘘嘘汇集6| 亚洲成人免费电影在线观看| 国产成人av教育| 午夜福利欧美成人| 日本一本二区三区精品| 国产一区二区三区在线臀色熟女| 国产黄片美女视频| 1024手机看黄色片| 亚洲成人精品中文字幕电影| 国产 一区 欧美 日韩| 有码 亚洲区| 亚洲欧美日韩高清在线视频| 久久久久久久久久黄片| 99热这里只有精品一区| 亚洲欧美日韩东京热| 1000部很黄的大片| 免费看美女性在线毛片视频| 赤兔流量卡办理| 国产精品三级大全| 中文字幕高清在线视频| 久久精品影院6| av专区在线播放| 床上黄色一级片| 在线国产一区二区在线| or卡值多少钱| 亚洲性久久影院| 亚洲av一区综合| 日韩,欧美,国产一区二区三区 | 成人二区视频| 亚洲成人中文字幕在线播放| 亚洲男人的天堂狠狠| 久久精品国产亚洲av香蕉五月| 天堂影院成人在线观看| 久久99热这里只有精品18| 极品教师在线视频| 久久精品夜夜夜夜夜久久蜜豆| 午夜福利视频1000在线观看| 欧美最新免费一区二区三区| 久久6这里有精品| 欧美最新免费一区二区三区| 97热精品久久久久久| 一级黄色大片毛片| 99国产精品一区二区蜜桃av| 三级男女做爰猛烈吃奶摸视频| 欧美xxxx黑人xx丫x性爽| 欧美精品啪啪一区二区三区| а√天堂www在线а√下载| 国产精品乱码一区二三区的特点| 99久久无色码亚洲精品果冻| 欧美三级亚洲精品| 久久午夜福利片| 欧美激情久久久久久爽电影| 亚洲aⅴ乱码一区二区在线播放| .国产精品久久| 久久国内精品自在自线图片| 婷婷精品国产亚洲av| 毛片一级片免费看久久久久 | 男人舔女人下体高潮全视频| 成人美女网站在线观看视频| 我要搜黄色片| 免费观看在线日韩| 最新在线观看一区二区三区| 美女高潮喷水抽搐中文字幕| 中文资源天堂在线| 性色avwww在线观看| 2021天堂中文幕一二区在线观| 久久久久久久久久久丰满 | 最近最新中文字幕大全电影3| 99九九线精品视频在线观看视频| 国产白丝娇喘喷水9色精品| 男人和女人高潮做爰伦理| 色在线成人网| 亚洲av成人精品一区久久| 舔av片在线| 全区人妻精品视频| 成年女人毛片免费观看观看9| 亚洲av美国av| 欧美激情国产日韩精品一区| 99在线视频只有这里精品首页| 午夜福利欧美成人| 午夜视频国产福利| 欧美xxxx黑人xx丫x性爽| 亚洲 国产 在线| 黄色丝袜av网址大全| 噜噜噜噜噜久久久久久91| 免费无遮挡裸体视频| 热99re8久久精品国产| 国产伦精品一区二区三区视频9| 啦啦啦韩国在线观看视频| 男女视频在线观看网站免费| 大型黄色视频在线免费观看| 又爽又黄a免费视频| 亚洲av一区综合| 麻豆久久精品国产亚洲av| 成人亚洲精品av一区二区| a级毛片免费高清观看在线播放| 婷婷精品国产亚洲av| 国产日本99.免费观看| 88av欧美| 少妇熟女aⅴ在线视频| 91久久精品国产一区二区成人| 午夜福利高清视频| 亚洲不卡免费看| 俄罗斯特黄特色一大片| av.在线天堂| 欧美成人免费av一区二区三区| 床上黄色一级片| 精品福利观看| 成年免费大片在线观看| 欧美精品国产亚洲| 网址你懂的国产日韩在线| 99热这里只有是精品在线观看| 日韩欧美三级三区| 亚洲国产精品合色在线| 亚洲人与动物交配视频| www.色视频.com| 搞女人的毛片| 亚洲黑人精品在线| 亚洲精品一区av在线观看| 欧美国产日韩亚洲一区| 色综合站精品国产| 欧美三级亚洲精品| 婷婷丁香在线五月| 噜噜噜噜噜久久久久久91| 在线天堂最新版资源| 成人亚洲精品av一区二区| 日韩强制内射视频| 国产一级毛片七仙女欲春2| 校园春色视频在线观看| 九九热线精品视视频播放| 精品一区二区三区视频在线观看免费| 免费看av在线观看网站| 长腿黑丝高跟| 女同久久另类99精品国产91| 亚洲一区高清亚洲精品| 变态另类丝袜制服| 欧美区成人在线视频|