• <tr id="yyy80"></tr>
  • <sup id="yyy80"></sup>
  • <tfoot id="yyy80"><noscript id="yyy80"></noscript></tfoot>
  • 99热精品在线国产_美女午夜性视频免费_国产精品国产高清国产av_av欧美777_自拍偷自拍亚洲精品老妇_亚洲熟女精品中文字幕_www日本黄色视频网_国产精品野战在线观看 ?

    Association of CT perfusion imaging with plasma levels of TGF-β1 and VEGF in patients with NSCLC

    2016-11-29 12:06:06DaWeiLiBaoZhongWuYuSenShiZhiQunLiXuDongLiuXiaoHuaLi

    Da-Wei Li, Bao-Zhong Wu, Yu-Sen Shi, Zhi-Qun Li, Xu-Dong Liu, Xiao-Hua Li

    Radiology Department, Affiliated Hospital of Hainan Medical College, Hainan, Haikou 570102, China

    Association of CT perfusion imaging with plasma levels of TGF-β1 and VEGF in patients with NSCLC

    Da-Wei Li, Bao-Zhong Wu, Yu-Sen Shi*, Zhi-Qun Li, Xu-Dong Liu, Xiao-Hua Li

    Radiology Department, Affiliated Hospital of Hainan Medical College, Hainan, Haikou 570102, China

    ARTICLE INFO

    Article history:

    Received 15 November 2015

    Received in revised form 20 December 2015

    Accepted 15 January 2016

    Available online 20 February 2016

    Non small cell lung cancer

    CT perfusion parameters

    Transforming growth factor-β1

    Vascular endothelial growth

    Objective: To study the association of CT perfusion imaging parameters with plasma level of transforming growth factor-β1 (TGF-β1) and vascular endothelial growth (VEGF) in patients with non small cell cancer (NSCLC). Methods: A total of 67 patients with NSCLC (NSCLC group) and 64 patients with benign lesion (control group) were given with CT perfusion imaging to obtain blood flow, blood volume, mean transit time, time to peal and permeability surface through CT perfusion software. The plasma levels of TGF-β1 and VEGF were tested by ELISA. The relationship between plasma levels of TGF-β1, VEGF and CT perfusion imaging parameters were analyzed. Results: CT perfusion imaging parameters and the plasma levels of TGF-β1 and VEGF of NSCLC group were significantly higher than the control group (P<0.05), while CT perfusion parameters and the levels of TGF-β1 and VEGF in NSCLC group showed significant difference in different tumor node metastasis stages (P<0.05). Correlation analysis showed that the level of plasma TGF-β1 and VEGF were positively correlated with blood flow, blood volume, and mean transit time (P<0.05), and negatively correlated with time to peal (P<0.05). There was no significant correlation between TGF-β 1 and VEGF with the permeability surface. Conclusions: CT perfusion imaging parameters in patients with NSCLC is closely associated with plasma TGF-β1, VEGF and its biological characteristics. CT perfusion imaging is a convenient method to detect tumor blood perfusion.

    1. Introduction

    Nowadays, the morbidity and mortality of lung cancer is increasing year by year. Eighty percentage of patients with lung cancer have non small cell lung cancer (NSCLC)[1]. Angiopoiesis affects the occurrence and development of tumor. It can slow down growth and can terminate metastasis if angiopoiesis of new vessels is blocked. Transforming growth factor-β (TGF-β1) and vascular endothelial growth (VEGF) play important roles in regulation of angiopoiesis[2]. Multi-slice spiral computed tomography perfusion imaging is a mature functional imaging, and has been widely used in observing density and distribution of tumor vessels, evaluating association with tumor metastasis related genes. In this study, we observed CT perfusion imaging parameters, including blood flow (BF), bloodvolume (BV), mean transit time (MTT), time to peal (TTP) and permeability surface (PS), analyzed the relationship between these parameters with TGF-β1 and VEGF, to discuss the valued of these parameter in evaluating NSCLC.

    2. Materials and methods

    2.1. Objective

    A total of 67 cases diagnosed as NSCLC by puncture or postoperation pathological diagnosis were selected as NSCLC group, who were admitted during January 2010 to January 2015. Another 60 cases with benign lesion were selected as control group. There were 40 males and 27 females in NSCLC group, aged 33-78 years old, with average age as (50.8±10.5) years. There were 36 cases with squamous carcinoma, 23 cases with adenocarcinoma, and 8 cases with adenoid squamous cell carcinoma. Among 64 cases in control group, there were 37 cases with tuberculosis focus, 13 caseswith pulmonary inflammatory pseudotumor, 9 cases with silicosis nodule, and 5 cases with lung infection. The general date such as age, gender, etc were comparable in both groups.

    2.2. Inclusion and exclusion criteria

    Inclusion criteria: (1)Patients were diagnosed as NSCLC by bronchofibroscopy biopsy, needle biopsy or post-operation pathological diagnosis, and had no chemoradiotherapy or anti-tumor drug; (2)Patients were able to have CT perfusion scanning.

    Exclusion criteria: (1)Sensitive to iodinated contrast agent; (2) breath-holding time were shorter than 25 s, or artifact appeared due to various reasons; (3)Patients with serious dysfunction of heart, liver or kidney, or with infection diseases; (4)Patients were diagnosed as small cell lung cancer by pathological examination.

    2.3. CT perfusion imaging

    Patients underwent CT plain scanning to identify lesion location. With the maximum lay of tumor as the center, dynamic scanning was performed in multi-level continuous dynamic movie scan model. The condition was as follows: 120 kV, 50 mA, detector width was 24 mm 1.2 mm; coverage area of Z-axis was 40 mm; thickness of reconstruction was 5 mm; average interval time was 1.0 s; rescanned 5 s later; 320 pieces of imagings were obtained after continuous collection for 40 s. Patlak method and maximum-slope method were used to calculate parameters, including BF, BV, MTT, TTP, PS, etc.

    2.4. Collection and detection of blood

    2.4.1. Collection of blood

    Five mL elbow venous blood were extracted at 8:00 am for fasting subjects in both groups. Anti-freezing was carried out immediately with ethylenediaminetetraacetic acid. It was centrifugated at 3 600 r/min for 10 min. Supernatant was obtained by pipettor, and preserved at -80 ℃.

    2.4.2. Detection of TGF-β1and VEGF

    Enzyme linked immunosorbent assay was used to detect content of TGF-β1and VEGF. The assays were provided by Aikang Biotechnology Compamy (Hangzhou, China). The process was in accord with instruction. Two holes were detected for every sample, and the average was calculated.

    2.5. Statistical analysis

    The data was processed by SPSS19.0. t test was used in comparison between groups. Pearson correlation analysis was used to analyze the correlation between parameters with TGF-β1and VEGF.

    Table 1Comparison of parameters between two groups.

    3. Results

    3.1. Parameters of two groups

    Table 1 showed BF (mL·100 mL-1·min-1), BV (mL/1 000), MTT (s), TTP (s) and PS (mL·100 mL-1·min-1) were significantly higher in NSCLC group (P<0.05).

    3.2. TGF-β1and VEGF levels

    Table 2 showed that TGF-β1and VEGF levels of NSCLC group were significantly higher than those of control group (P<0.01).

    Table 2TGF-β1and VEGF levels (pg/mL).

    3.3. Parameters, TGF-β1and VEGF levels of NSCLC patients at different stage

    All 67 NSCLC patients were divided into ⅠA-ⅡA stage group (n=23, Group 1), ⅡB-ⅢA stage group (n=27, Group 2), ⅢB-Ⅳstage group (n=17, Group 3) according to clinical and CT examination result. CT perfusion parameters, TGF-β1and VEGF levels were observed. There were significant differences in parameters, TGF-β1and VEGF levels between group 1 and group 2 (P<0.05), and the differences were also significant between group 1 and group 3 (P<0.05). However, the difference was not significant between group 2 and group 3 (P>0.05).

    Table 3Parameters, TGF-β1and VEGF levels of NSCLC patients at different stage.

    3.4. Correlation between parameter with TGF-β1and VEGF

    Pearson linear correlation analysis showed that the level of plasmaTGF-β1 and VEGF were positively correlated with BF, BV, and MTT (P<0.05), and negatively correlated with TTP (P<0.05). There was no significant correlation between TGF-β1 and VEGF with the PS (Table 4).

    Table 4Correlation between parameter with TGF-β1and VEGF.

    4. Discussion

    NSCLC is the commonest type of lung tumors. The complicated biological processes such as occurrence, development, metastasis etc are associated with angiopoiesis. Angiopoiesis of tumor vessels includes angiopoiesis induced by tumor cells and intratumor microcirculation, which is achieved by angiopoiesis and vasculogenesis. Vasculogenesis refers to differentiation of mesoblastema into haemangioblast, which is predecessor of endothelial cell, and constitute embryo primitive vascular system. In this stage, the growth of tumor is slow, and shows no invasiveness, with few metastases[3]. Angiopoiesis refers to growth of existedcapillary or of new capillary in postcapillary venule. It can obtain various nutritions for growth, and is liable to infiltration and metastasis. Therefore, it is easy for tumor to growth in area with rich new vessels and sufficient blood supply[4].

    TGF-β super family-mediated signal path can regulate the occurrence, development, metastasis of tumors. It can stimulate the growth of vessels. There are three types of TGF-β for mammal animals, and the content of TGF-β1 is the highest. TGF-β1 is representative. It can stimulate vessel growth directly or indirectly, which may be correlated with angiopoiesis and development[5]. It is reported that malignant tumor cells can secret plenty of cytokines during tumor development, which lead to rapid proliferation of malignant tumor, and higher expression of TGF-β1[6]. Besides, VEGF is a glycoprotein with many functions. It can stimulate proliferation and migration of vascular endothelial cell. And it is also the strongest vascular permeability agent. It can lead to pathological angiopoiesis, including increasing mitosis and migration of vascular endothelial cell[7,8].

    CT perfusion imaging is a non-invasive method to evaluate growth of microvessels in tumor tissues, then to reflect physiological and metabolic changes via detecting various parameters. Besides, it can also indicate clinical change characters of tumors before any change in size occurs or just when tiny change occurs. Tacelli et al found that APA detected by dynamic contrast-enhance spiral CT is positively correlated with VEGF expression of adenocarcinoma. BF, BV, MTT, TTP and PS can reflect microcirculation characters from various aspects[9]. This study showed that the level of plasma TGF-β1 and VEGF were positively correlated with BF, BV, and MTT, and negatively correlated with TTP. It indicates that TGF-β 1 and VEGF expressions are increased, new vessels are increased and circulation is also increased. From macroscopic aspect, CT

    imaging is characterized as increased blood flow in per unit volume of tumor tissue per unit time (BF), increased blood volume in per unit volume of tumor tissue (BV), and delayed time for contrast agent passing tissue capillary[10]. However, PS which indicates permeability surface and integrity of capillary, shows no significant correlation with TGF-β1 or VEGF. Tumor node metastasis staging reflects tumor progress degree. The higher the stage is, the severer the progress is, the wider the affected tissue. This progress is closely correlated with angiopoiesis. Our study showed the difference in parameters between ⅡB-ⅢA and ⅢB-Ⅳ is more significant thanⅠA -ⅡA. This trend is consistent with TGF-β1 and VEGF levels in plasma. It indicates that CT perfusion imaging not only can be used in diagnosis and differentiation of benign and malignant tumors, but also can be used in NSCLC staging and prognosis.

    In conclusion, a plenty of angiogenesis occurs during tumor development. It can lead to increased TGF-β1 and VEGF levels. CT perfusion imaging can reflect density and structure of microvascular system from imaging aspect. The combination of both is valuable in diagnosis, differentiation, treatment and prognosis.

    Conflicts of interest statement

    The authors have no conflicts of interest.

    [1] Das M, Wakelee H. Angiogenesis and lung cancer: ramucirumab prolongs survival in 2(nd)-line metastatic NSCLC. Transl Lung Cancer Res 2014; 3(6): 397-399.

    [2] Engels EA, Jennings L, Kemp TJ, Chaturvedi AK, Pinto LA, Pfeiffer RM, et al. Circulating TGF-β1 and VEGF and risk of cancer among liver transplant recipients. Cancer Med 2015; 4(8): 1252-1257.

    [3] Lee JE, Kim C, Yang H, Park I, Oh N, Hua S. Novel glycosylated VEGF decoy receptor fusion protein, VEGF-Grab, efficiently suppresses tumor angiogenesis and progression. Mol Cancer Ther 2015; 14(2): 470-479.

    [4] Saini R, Hoyt K. Recent developments in dynamic contrast-enhanced ultrasound imaging of tumor angiogenesis. Imaging Med 2014; 6(1): 41-52.

    [5] Ryzhov SV, Pickup MW, Chytil A, Gorska AE, Zhang QKm Owens P, et al. Role of TGF-β signaling in generation of CD39+CD73+myeloid cells in tumors. J Immunol 2014; 193(6): 3155-3164.

    [6] Kim JW, Koh Y, Kim DW, Ahn YO, Kim TM, Han SW, et al. Clinical implications of VEGF, TGF-β1, and IL-1β in patients with advanced non-small cell lung cancer. Cancer Res Treat 2013; 45(4): 325-333.

    [7] Alevizakos M, Kaltsas S, Syrigos KN. The VEGF pathway in lung cancer. Cancer Chemother Pharmacol 2013; 72(6): 1169-1181.

    [8] Smith NR, Wedge SR, Pommier A, Barry ST. Mechanisms that influence tumour response to VEGF-pathway inhibitors. Biochem Soc Trans 2014; 42(6): 1601-1607.

    [9] Tacelli N, Santangelo T, Scherpereel A, Alain Duhamel, Valérie Deken, Ernst Klotz, et al. Perfusion CT allows prediction of therapy response in non-small cell lung cancer treated with conventional and anti-angiogenic chemotherapy. Eur Radiol 2013; 23(8): 2127-2136.

    [10] Knobloch G, Jost G, Huppertz A, Pietsch H. Dual-energy computed tomography for the assessment of early treatment effects of regorafenib in a preclinical tumor model: comparison with dynamic contrastenhanced CT and conventional contrast-enhanced single-energy CT. Eur Radiol 2014; 24(8): 1896-1905.

    ent heading

    10.1016/j.apjtm.2016.01.009

    *Corresponding author: Yu-Sen Shi, Associate Chief Physician, Radiology Department, Affiliated Hospital of Hainan Medical College, Hainan, Haikou 570102, China.

    E-mail: sunbird709@126.com

    久久久久久亚洲精品国产蜜桃av| 久久精品aⅴ一区二区三区四区| 欧美精品亚洲一区二区| 91大片在线观看| 午夜精品久久久久久毛片777| 99热网站在线观看| 亚洲av日韩在线播放| 国产精品.久久久| 国产欧美日韩一区二区三 | av在线播放精品| 2018国产大陆天天弄谢| 一进一出抽搐动态| 一级片免费观看大全| 俄罗斯特黄特色一大片| 日韩欧美一区二区三区在线观看 | 国产在线视频一区二区| 久久亚洲国产成人精品v| 另类亚洲欧美激情| 精品视频人人做人人爽| 精品一区二区三卡| 在线观看一区二区三区激情| 三上悠亚av全集在线观看| 精品国产超薄肉色丝袜足j| a 毛片基地| 日韩免费高清中文字幕av| 精品一区在线观看国产| 一本—道久久a久久精品蜜桃钙片| 各种免费的搞黄视频| 亚洲一卡2卡3卡4卡5卡精品中文| 18在线观看网站| 久久免费观看电影| 在线观看免费日韩欧美大片| 国产成人影院久久av| a在线观看视频网站| 色精品久久人妻99蜜桃| 亚洲av日韩在线播放| 满18在线观看网站| 啪啪无遮挡十八禁网站| 51午夜福利影视在线观看| 满18在线观看网站| 纵有疾风起免费观看全集完整版| 51午夜福利影视在线观看| 叶爱在线成人免费视频播放| 亚洲av日韩精品久久久久久密| kizo精华| 一二三四社区在线视频社区8| 亚洲精品美女久久久久99蜜臀| 精品乱码久久久久久99久播| 国产不卡av网站在线观看| 精品人妻在线不人妻| 在线观看免费视频网站a站| 久热这里只有精品99| 精品视频人人做人人爽| 国产不卡av网站在线观看| 久热这里只有精品99| 80岁老熟妇乱子伦牲交| 80岁老熟妇乱子伦牲交| 91精品三级在线观看| 欧美激情极品国产一区二区三区| 人人妻人人澡人人爽人人夜夜| 成人亚洲精品一区在线观看| 成在线人永久免费视频| 最近最新中文字幕大全免费视频| a级毛片在线看网站| 十八禁人妻一区二区| 欧美成狂野欧美在线观看| 欧美日韩视频精品一区| 欧美日韩一级在线毛片| 操出白浆在线播放| 少妇人妻久久综合中文| 国产97色在线日韩免费| 精品欧美一区二区三区在线| 成人影院久久| 日本av手机在线免费观看| 精品少妇内射三级| 老司机亚洲免费影院| 黑人巨大精品欧美一区二区mp4| 欧美日韩黄片免| 中文字幕制服av| 极品少妇高潮喷水抽搐| 国产成人系列免费观看| 亚洲国产看品久久| 18禁国产床啪视频网站| 美女高潮喷水抽搐中文字幕| 亚洲精品国产精品久久久不卡| 99精国产麻豆久久婷婷| 天堂中文最新版在线下载| 久久免费观看电影| 久久香蕉激情| 日韩大片免费观看网站| 老司机福利观看| 正在播放国产对白刺激| 色视频在线一区二区三区| 久久青草综合色| netflix在线观看网站| 久久久久网色| 亚洲精品在线美女| 91成人精品电影| 精品一品国产午夜福利视频| 日本精品一区二区三区蜜桃| 成人亚洲精品一区在线观看| 母亲3免费完整高清在线观看| 免费高清在线观看视频在线观看| 一级黄色大片毛片| 小说图片视频综合网站| 亚洲一区二区三区不卡视频| 人人妻人人看人人澡| 欧美国产日韩亚洲一区| 91老司机精品| 国产99久久九九免费精品| 男男h啪啪无遮挡| 又黄又粗又硬又大视频| 婷婷精品国产亚洲av| 身体一侧抽搐| 亚洲精品一区av在线观看| 久久精品国产亚洲av香蕉五月| 亚洲av成人av| 叶爱在线成人免费视频播放| 久久亚洲真实| 久久精品成人免费网站| 精品欧美国产一区二区三| 桃色一区二区三区在线观看| 午夜成年电影在线免费观看| 国产精品一区二区免费欧美| 男男h啪啪无遮挡| 好看av亚洲va欧美ⅴa在| 黄色成人免费大全| 精品国产超薄肉色丝袜足j| 男女午夜视频在线观看| 在线永久观看黄色视频| 国产一区二区在线av高清观看| 51午夜福利影视在线观看| 久久香蕉激情| 亚洲avbb在线观看| 最新美女视频免费是黄的| 国产伦在线观看视频一区| 久热爱精品视频在线9| 亚洲专区中文字幕在线| 五月伊人婷婷丁香| 免费高清视频大片| 免费看美女性在线毛片视频| 狠狠狠狠99中文字幕| 国产精华一区二区三区| 99久久精品国产亚洲精品| 欧美大码av| 欧美日韩国产亚洲二区| 欧美日韩黄片免| 97人妻精品一区二区三区麻豆| 亚洲熟妇中文字幕五十中出| 国产亚洲精品久久久久5区| 久久精品成人免费网站| 亚洲人成伊人成综合网2020| 在线播放国产精品三级| 亚洲国产欧美网| 熟女电影av网| 神马国产精品三级电影在线观看 | 99久久精品国产亚洲精品| 欧美日韩亚洲国产一区二区在线观看| 人妻丰满熟妇av一区二区三区| 欧美性长视频在线观看| 欧美三级亚洲精品| 村上凉子中文字幕在线| 亚洲av成人av| 亚洲黑人精品在线| 男女视频在线观看网站免费 | 亚洲精品中文字幕在线视频| 国产精品,欧美在线| 波多野结衣高清作品| netflix在线观看网站| 99国产精品99久久久久| 亚洲最大成人中文| 亚洲成人久久性| 国产精品av视频在线免费观看| 琪琪午夜伦伦电影理论片6080| 人成视频在线观看免费观看| x7x7x7水蜜桃| 夜夜躁狠狠躁天天躁| 国产精品一区二区三区四区免费观看 | 一个人观看的视频www高清免费观看 | 老司机靠b影院| 亚洲精品在线观看二区| 国产主播在线观看一区二区| av有码第一页| 久久精品91无色码中文字幕| 黄色片一级片一级黄色片| svipshipincom国产片| 成人精品一区二区免费| 成人国产综合亚洲| 此物有八面人人有两片| 国产欧美日韩一区二区三| 一个人免费在线观看的高清视频| 一级毛片精品| 精品不卡国产一区二区三区| 三级国产精品欧美在线观看 | 两个人的视频大全免费| av在线播放免费不卡| 午夜福利免费观看在线| 最近最新免费中文字幕在线| 国产视频内射| 国产成人av激情在线播放| 亚洲午夜精品一区,二区,三区| 国产精品综合久久久久久久免费| 久久这里只有精品中国| 久久久久久大精品| 亚洲成av人片免费观看| 九色国产91popny在线| 变态另类成人亚洲欧美熟女| 2021天堂中文幕一二区在线观| 久久久久久久久久黄片| 色精品久久人妻99蜜桃| 欧美成人免费av一区二区三区| 麻豆一二三区av精品| 又紧又爽又黄一区二区| 久久久国产欧美日韩av| 波多野结衣高清作品| 最近最新免费中文字幕在线| 可以免费在线观看a视频的电影网站| 中文字幕高清在线视频| 亚洲成人免费电影在线观看| 亚洲精品久久成人aⅴ小说| 亚洲中文av在线| 俺也久久电影网| 蜜桃久久精品国产亚洲av| 久久精品亚洲精品国产色婷小说| 一区二区三区高清视频在线| 丰满人妻一区二区三区视频av | 国产激情久久老熟女| 久久精品亚洲精品国产色婷小说| 18禁观看日本| 99精品欧美一区二区三区四区| 男女视频在线观看网站免费 | 日韩精品青青久久久久久| 欧美黄色片欧美黄色片| 亚洲五月天丁香| www.精华液| 久久久久国产精品人妻aⅴ院| 国产精品综合久久久久久久免费| 男女床上黄色一级片免费看| 国产精品 国内视频| 国产成人欧美在线观看| 99国产精品99久久久久| 人妻夜夜爽99麻豆av| 99精品欧美一区二区三区四区| 99久久无色码亚洲精品果冻| 久久久久国产一级毛片高清牌| 久久久久久久久中文| 两个人的视频大全免费| 国产精品,欧美在线| 国产精品 国内视频| 91麻豆av在线| 无限看片的www在线观看| 91字幕亚洲| 88av欧美| 日韩大码丰满熟妇| 久久久久精品国产欧美久久久| 成人国产综合亚洲| 国产成人系列免费观看| 亚洲精品中文字幕一二三四区| 日本撒尿小便嘘嘘汇集6| 日日摸夜夜添夜夜添小说| 欧美日韩黄片免| 免费在线观看完整版高清| 久久久久国内视频| √禁漫天堂资源中文www| 成人国产综合亚洲| 成人三级黄色视频| 久久久精品欧美日韩精品| 久99久视频精品免费| 怎么达到女性高潮| 在线观看www视频免费| 国产精品免费一区二区三区在线| 国产精品久久视频播放| 日本三级黄在线观看| 免费无遮挡裸体视频| 国产真人三级小视频在线观看| 黄频高清免费视频| 午夜老司机福利片| 一级毛片高清免费大全| 一进一出抽搐gif免费好疼| 五月玫瑰六月丁香| 禁无遮挡网站| 亚洲人成网站高清观看| 国产一级毛片七仙女欲春2| 亚洲激情在线av| 中国美女看黄片| 国产激情久久老熟女| 岛国在线观看网站| 婷婷六月久久综合丁香| 亚洲国产欧美一区二区综合| 亚洲国产中文字幕在线视频| 激情在线观看视频在线高清| 亚洲乱码一区二区免费版| 99国产综合亚洲精品| 国产97色在线日韩免费| 搞女人的毛片| 免费观看精品视频网站| 亚洲av五月六月丁香网| 久久精品夜夜夜夜夜久久蜜豆 | 91麻豆av在线| 亚洲av五月六月丁香网| 国产av一区二区精品久久| www.www免费av| 国内久久婷婷六月综合欲色啪| 免费无遮挡裸体视频| www日本黄色视频网| 淫秽高清视频在线观看| 热99re8久久精品国产| 亚洲av片天天在线观看| 日韩免费av在线播放| 亚洲熟妇熟女久久| 免费在线观看成人毛片| 91九色精品人成在线观看| av中文乱码字幕在线| 国产精品av久久久久免费| 老司机午夜十八禁免费视频| 怎么达到女性高潮| 又粗又爽又猛毛片免费看| 成在线人永久免费视频| 精品无人区乱码1区二区| 欧美性长视频在线观看| 精品久久久久久,| 一夜夜www| 99热这里只有精品一区 | 成年女人毛片免费观看观看9| 免费在线观看视频国产中文字幕亚洲| avwww免费| 男人的好看免费观看在线视频 | 日韩欧美国产一区二区入口| 国产一区二区三区在线臀色熟女| 国产成人一区二区三区免费视频网站| 黄色毛片三级朝国网站| 婷婷精品国产亚洲av在线| 91成年电影在线观看| 久久精品国产99精品国产亚洲性色| 亚洲欧美日韩东京热| 久久亚洲精品不卡| 精品久久久久久久末码| 欧美成人免费av一区二区三区| 手机成人av网站| 免费av毛片视频| 午夜免费激情av| 夜夜躁狠狠躁天天躁| 神马国产精品三级电影在线观看 | 天堂av国产一区二区熟女人妻 | 叶爱在线成人免费视频播放| 日本撒尿小便嘘嘘汇集6| 亚洲自偷自拍图片 自拍| 国内揄拍国产精品人妻在线| 一级片免费观看大全| 99热这里只有是精品50| 91字幕亚洲| 成人午夜高清在线视频| svipshipincom国产片| 精品国产亚洲在线| 麻豆国产97在线/欧美 | 在线观看舔阴道视频| 18禁黄网站禁片免费观看直播| 亚洲五月天丁香| 脱女人内裤的视频| 日韩精品青青久久久久久| 久久精品国产亚洲av高清一级| 最新美女视频免费是黄的| 亚洲专区中文字幕在线| 在线观看美女被高潮喷水网站 | 日本一二三区视频观看| 亚洲在线自拍视频| 亚洲专区中文字幕在线| 久久人妻福利社区极品人妻图片| 亚洲国产中文字幕在线视频| 久久婷婷成人综合色麻豆| 欧美黑人欧美精品刺激| 日韩有码中文字幕| 女人爽到高潮嗷嗷叫在线视频| 午夜影院日韩av| cao死你这个sao货| 国产成人av激情在线播放| 嫩草影视91久久| 最近最新中文字幕大全免费视频| 高清在线国产一区| 999久久久精品免费观看国产| 女生性感内裤真人,穿戴方法视频| 国产成人一区二区三区免费视频网站| 亚洲va日本ⅴa欧美va伊人久久| 宅男免费午夜| 可以在线观看的亚洲视频| 岛国在线观看网站| 天堂动漫精品| 少妇的丰满在线观看| 成人特级黄色片久久久久久久| 国产一区二区在线av高清观看| 女人被狂操c到高潮| 精品不卡国产一区二区三区| 日韩三级视频一区二区三区| 最新在线观看一区二区三区| 亚洲精品一卡2卡三卡4卡5卡| 91成年电影在线观看| 伦理电影免费视频| www.自偷自拍.com| 欧美zozozo另类| 人妻丰满熟妇av一区二区三区| 久久国产乱子伦精品免费另类| 国模一区二区三区四区视频 | 九色国产91popny在线| 神马国产精品三级电影在线观看 | 国产成人精品久久二区二区免费| 色噜噜av男人的天堂激情| 99精品久久久久人妻精品| 看黄色毛片网站| 国产精品av视频在线免费观看| 麻豆国产av国片精品| 国产亚洲精品一区二区www| 欧美午夜高清在线| 精品无人区乱码1区二区| av福利片在线观看| 天天添夜夜摸| 欧美久久黑人一区二区| 999久久久国产精品视频| 欧美日韩亚洲综合一区二区三区_| 国产成人av激情在线播放| 国产av在哪里看| 又大又爽又粗| netflix在线观看网站| 精品国产美女av久久久久小说| 亚洲人成伊人成综合网2020| 亚洲欧美日韩东京热| 亚洲av成人av| 亚洲成a人片在线一区二区| 又紧又爽又黄一区二区| av欧美777| 1024视频免费在线观看| 高清在线国产一区| 老熟妇乱子伦视频在线观看| 久久精品国产清高在天天线| 国产成人一区二区三区免费视频网站| 亚洲va日本ⅴa欧美va伊人久久| 亚洲熟妇中文字幕五十中出| 中出人妻视频一区二区| а√天堂www在线а√下载| 久久香蕉激情| 欧美精品亚洲一区二区| 亚洲av日韩精品久久久久久密| 午夜久久久久精精品| 人人妻,人人澡人人爽秒播| 1024视频免费在线观看| 国产伦一二天堂av在线观看| 中文字幕人妻丝袜一区二区| 一卡2卡三卡四卡精品乱码亚洲| 一进一出抽搐gif免费好疼| 欧美zozozo另类| 亚洲成av人片在线播放无| 在线免费观看的www视频| 老司机福利观看| 国产精品久久久久久亚洲av鲁大| 俺也久久电影网| netflix在线观看网站| 99热只有精品国产| 久久草成人影院| 好男人电影高清在线观看| 久久亚洲真实| 久久久久免费精品人妻一区二区| 99热只有精品国产| 日本三级黄在线观看| 国产av在哪里看| 别揉我奶头~嗯~啊~动态视频| 曰老女人黄片| 亚洲性夜色夜夜综合| 成人一区二区视频在线观看| 日本 av在线| 国产单亲对白刺激| 天天躁夜夜躁狠狠躁躁| 国产成人精品久久二区二区91| 日日摸夜夜添夜夜添小说| 午夜a级毛片| 久久久久性生活片| 欧美一区二区精品小视频在线| 午夜免费激情av| 欧美不卡视频在线免费观看 | 国产1区2区3区精品| 国产精品免费一区二区三区在线| 精品久久蜜臀av无| 国产精品影院久久| 欧美乱妇无乱码| 久热爱精品视频在线9| 18禁美女被吸乳视频| 久久久久久久久中文| 亚洲av美国av| 久久伊人香网站| 五月伊人婷婷丁香| 无人区码免费观看不卡| 国产99白浆流出| 久久久久九九精品影院| 两性午夜刺激爽爽歪歪视频在线观看 | 国产亚洲精品久久久久5区| 国产av麻豆久久久久久久| 五月玫瑰六月丁香| 日本在线视频免费播放| 18禁黄网站禁片免费观看直播| e午夜精品久久久久久久| 欧美+亚洲+日韩+国产| tocl精华| 在线观看www视频免费| 18禁裸乳无遮挡免费网站照片| 欧美激情久久久久久爽电影| 99国产综合亚洲精品| 一区二区三区国产精品乱码| 久久久久久国产a免费观看| 一级毛片女人18水好多| 他把我摸到了高潮在线观看| 精品午夜福利视频在线观看一区| 成人三级黄色视频| 欧美丝袜亚洲另类 | 午夜精品一区二区三区免费看| 欧美午夜高清在线| 久久久久精品国产欧美久久久| av国产免费在线观看| 欧美一级毛片孕妇| 少妇人妻一区二区三区视频| 桃色一区二区三区在线观看| 欧美又色又爽又黄视频| 最近在线观看免费完整版| 国产午夜福利久久久久久| 午夜免费激情av| 国产亚洲精品久久久久久毛片| 免费看十八禁软件| 熟女电影av网| 在线观看免费日韩欧美大片| 亚洲成人免费电影在线观看| 精品久久久久久久末码| 久久国产精品影院| 麻豆av在线久日| 婷婷亚洲欧美| 国产精品久久久久久人妻精品电影| 亚洲国产欧美网| 久久久久久久午夜电影| 真人做人爱边吃奶动态| 90打野战视频偷拍视频| 中文字幕av在线有码专区| 久久久国产精品麻豆| 黄色毛片三级朝国网站| 国产一区二区激情短视频| 国产激情偷乱视频一区二区| 久久久国产精品麻豆| 精品高清国产在线一区| 久久久国产精品麻豆| 国产99久久九九免费精品| 亚洲精品美女久久久久99蜜臀| 亚洲人成网站高清观看| 五月玫瑰六月丁香| 午夜精品一区二区三区免费看| 日本精品一区二区三区蜜桃| 黄色a级毛片大全视频| 美女高潮喷水抽搐中文字幕| 国产在线精品亚洲第一网站| 精品乱码久久久久久99久播| 国产精品一区二区免费欧美| 丰满人妻一区二区三区视频av | 女人高潮潮喷娇喘18禁视频| 在线看三级毛片| 少妇人妻一区二区三区视频| 成人亚洲精品av一区二区| 欧美在线黄色| 亚洲国产中文字幕在线视频| 一区福利在线观看| 黄色丝袜av网址大全| 日韩欧美三级三区| 夜夜躁狠狠躁天天躁| 琪琪午夜伦伦电影理论片6080| 精品高清国产在线一区| 成在线人永久免费视频| 女人爽到高潮嗷嗷叫在线视频| 丝袜美腿诱惑在线| 免费在线观看视频国产中文字幕亚洲| 国产成人精品无人区| 老司机在亚洲福利影院| 一个人观看的视频www高清免费观看 | 99久久无色码亚洲精品果冻| 亚洲国产精品合色在线| 岛国在线免费视频观看| 亚洲色图av天堂| 国产成人精品无人区| 久热爱精品视频在线9| 国产成人影院久久av| 欧美日韩黄片免| 久9热在线精品视频| 日韩免费av在线播放| 精品一区二区三区四区五区乱码| 亚洲精品在线观看二区| 桃色一区二区三区在线观看| 国产真人三级小视频在线观看| 欧美极品一区二区三区四区| 久久精品国产亚洲av香蕉五月| 久久午夜综合久久蜜桃| 国产高清视频在线观看网站| 亚洲 欧美一区二区三区| 淫秽高清视频在线观看| 一本综合久久免费| 欧美在线一区亚洲| 午夜两性在线视频| 成人手机av| 午夜视频精品福利| 国产成人av教育| 亚洲成人精品中文字幕电影| 国产激情久久老熟女| 久久精品国产清高在天天线| 免费观看精品视频网站| 欧美性猛交黑人性爽| 国产亚洲精品一区二区www| 欧美zozozo另类| 女同久久另类99精品国产91| tocl精华| 国产av在哪里看| 特大巨黑吊av在线直播| 1024香蕉在线观看| 精品久久久久久久人妻蜜臀av| 日韩欧美免费精品|