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    Association of CT perfusion imaging with plasma levels of TGF-β1 and VEGF in patients with NSCLC

    2016-11-29 12:06:06DaWeiLiBaoZhongWuYuSenShiZhiQunLiXuDongLiuXiaoHuaLi

    Da-Wei Li, Bao-Zhong Wu, Yu-Sen Shi, Zhi-Qun Li, Xu-Dong Liu, Xiao-Hua Li

    Radiology Department, Affiliated Hospital of Hainan Medical College, Hainan, Haikou 570102, China

    Association of CT perfusion imaging with plasma levels of TGF-β1 and VEGF in patients with NSCLC

    Da-Wei Li, Bao-Zhong Wu, Yu-Sen Shi*, Zhi-Qun Li, Xu-Dong Liu, Xiao-Hua Li

    Radiology Department, Affiliated Hospital of Hainan Medical College, Hainan, Haikou 570102, China

    ARTICLE INFO

    Article history:

    Received 15 November 2015

    Received in revised form 20 December 2015

    Accepted 15 January 2016

    Available online 20 February 2016

    Non small cell lung cancer

    CT perfusion parameters

    Transforming growth factor-β1

    Vascular endothelial growth

    Objective: To study the association of CT perfusion imaging parameters with plasma level of transforming growth factor-β1 (TGF-β1) and vascular endothelial growth (VEGF) in patients with non small cell cancer (NSCLC). Methods: A total of 67 patients with NSCLC (NSCLC group) and 64 patients with benign lesion (control group) were given with CT perfusion imaging to obtain blood flow, blood volume, mean transit time, time to peal and permeability surface through CT perfusion software. The plasma levels of TGF-β1 and VEGF were tested by ELISA. The relationship between plasma levels of TGF-β1, VEGF and CT perfusion imaging parameters were analyzed. Results: CT perfusion imaging parameters and the plasma levels of TGF-β1 and VEGF of NSCLC group were significantly higher than the control group (P<0.05), while CT perfusion parameters and the levels of TGF-β1 and VEGF in NSCLC group showed significant difference in different tumor node metastasis stages (P<0.05). Correlation analysis showed that the level of plasma TGF-β1 and VEGF were positively correlated with blood flow, blood volume, and mean transit time (P<0.05), and negatively correlated with time to peal (P<0.05). There was no significant correlation between TGF-β 1 and VEGF with the permeability surface. Conclusions: CT perfusion imaging parameters in patients with NSCLC is closely associated with plasma TGF-β1, VEGF and its biological characteristics. CT perfusion imaging is a convenient method to detect tumor blood perfusion.

    1. Introduction

    Nowadays, the morbidity and mortality of lung cancer is increasing year by year. Eighty percentage of patients with lung cancer have non small cell lung cancer (NSCLC)[1]. Angiopoiesis affects the occurrence and development of tumor. It can slow down growth and can terminate metastasis if angiopoiesis of new vessels is blocked. Transforming growth factor-β (TGF-β1) and vascular endothelial growth (VEGF) play important roles in regulation of angiopoiesis[2]. Multi-slice spiral computed tomography perfusion imaging is a mature functional imaging, and has been widely used in observing density and distribution of tumor vessels, evaluating association with tumor metastasis related genes. In this study, we observed CT perfusion imaging parameters, including blood flow (BF), bloodvolume (BV), mean transit time (MTT), time to peal (TTP) and permeability surface (PS), analyzed the relationship between these parameters with TGF-β1 and VEGF, to discuss the valued of these parameter in evaluating NSCLC.

    2. Materials and methods

    2.1. Objective

    A total of 67 cases diagnosed as NSCLC by puncture or postoperation pathological diagnosis were selected as NSCLC group, who were admitted during January 2010 to January 2015. Another 60 cases with benign lesion were selected as control group. There were 40 males and 27 females in NSCLC group, aged 33-78 years old, with average age as (50.8±10.5) years. There were 36 cases with squamous carcinoma, 23 cases with adenocarcinoma, and 8 cases with adenoid squamous cell carcinoma. Among 64 cases in control group, there were 37 cases with tuberculosis focus, 13 caseswith pulmonary inflammatory pseudotumor, 9 cases with silicosis nodule, and 5 cases with lung infection. The general date such as age, gender, etc were comparable in both groups.

    2.2. Inclusion and exclusion criteria

    Inclusion criteria: (1)Patients were diagnosed as NSCLC by bronchofibroscopy biopsy, needle biopsy or post-operation pathological diagnosis, and had no chemoradiotherapy or anti-tumor drug; (2)Patients were able to have CT perfusion scanning.

    Exclusion criteria: (1)Sensitive to iodinated contrast agent; (2) breath-holding time were shorter than 25 s, or artifact appeared due to various reasons; (3)Patients with serious dysfunction of heart, liver or kidney, or with infection diseases; (4)Patients were diagnosed as small cell lung cancer by pathological examination.

    2.3. CT perfusion imaging

    Patients underwent CT plain scanning to identify lesion location. With the maximum lay of tumor as the center, dynamic scanning was performed in multi-level continuous dynamic movie scan model. The condition was as follows: 120 kV, 50 mA, detector width was 24 mm 1.2 mm; coverage area of Z-axis was 40 mm; thickness of reconstruction was 5 mm; average interval time was 1.0 s; rescanned 5 s later; 320 pieces of imagings were obtained after continuous collection for 40 s. Patlak method and maximum-slope method were used to calculate parameters, including BF, BV, MTT, TTP, PS, etc.

    2.4. Collection and detection of blood

    2.4.1. Collection of blood

    Five mL elbow venous blood were extracted at 8:00 am for fasting subjects in both groups. Anti-freezing was carried out immediately with ethylenediaminetetraacetic acid. It was centrifugated at 3 600 r/min for 10 min. Supernatant was obtained by pipettor, and preserved at -80 ℃.

    2.4.2. Detection of TGF-β1and VEGF

    Enzyme linked immunosorbent assay was used to detect content of TGF-β1and VEGF. The assays were provided by Aikang Biotechnology Compamy (Hangzhou, China). The process was in accord with instruction. Two holes were detected for every sample, and the average was calculated.

    2.5. Statistical analysis

    The data was processed by SPSS19.0. t test was used in comparison between groups. Pearson correlation analysis was used to analyze the correlation between parameters with TGF-β1and VEGF.

    Table 1Comparison of parameters between two groups.

    3. Results

    3.1. Parameters of two groups

    Table 1 showed BF (mL·100 mL-1·min-1), BV (mL/1 000), MTT (s), TTP (s) and PS (mL·100 mL-1·min-1) were significantly higher in NSCLC group (P<0.05).

    3.2. TGF-β1and VEGF levels

    Table 2 showed that TGF-β1and VEGF levels of NSCLC group were significantly higher than those of control group (P<0.01).

    Table 2TGF-β1and VEGF levels (pg/mL).

    3.3. Parameters, TGF-β1and VEGF levels of NSCLC patients at different stage

    All 67 NSCLC patients were divided into ⅠA-ⅡA stage group (n=23, Group 1), ⅡB-ⅢA stage group (n=27, Group 2), ⅢB-Ⅳstage group (n=17, Group 3) according to clinical and CT examination result. CT perfusion parameters, TGF-β1and VEGF levels were observed. There were significant differences in parameters, TGF-β1and VEGF levels between group 1 and group 2 (P<0.05), and the differences were also significant between group 1 and group 3 (P<0.05). However, the difference was not significant between group 2 and group 3 (P>0.05).

    Table 3Parameters, TGF-β1and VEGF levels of NSCLC patients at different stage.

    3.4. Correlation between parameter with TGF-β1and VEGF

    Pearson linear correlation analysis showed that the level of plasmaTGF-β1 and VEGF were positively correlated with BF, BV, and MTT (P<0.05), and negatively correlated with TTP (P<0.05). There was no significant correlation between TGF-β1 and VEGF with the PS (Table 4).

    Table 4Correlation between parameter with TGF-β1and VEGF.

    4. Discussion

    NSCLC is the commonest type of lung tumors. The complicated biological processes such as occurrence, development, metastasis etc are associated with angiopoiesis. Angiopoiesis of tumor vessels includes angiopoiesis induced by tumor cells and intratumor microcirculation, which is achieved by angiopoiesis and vasculogenesis. Vasculogenesis refers to differentiation of mesoblastema into haemangioblast, which is predecessor of endothelial cell, and constitute embryo primitive vascular system. In this stage, the growth of tumor is slow, and shows no invasiveness, with few metastases[3]. Angiopoiesis refers to growth of existedcapillary or of new capillary in postcapillary venule. It can obtain various nutritions for growth, and is liable to infiltration and metastasis. Therefore, it is easy for tumor to growth in area with rich new vessels and sufficient blood supply[4].

    TGF-β super family-mediated signal path can regulate the occurrence, development, metastasis of tumors. It can stimulate the growth of vessels. There are three types of TGF-β for mammal animals, and the content of TGF-β1 is the highest. TGF-β1 is representative. It can stimulate vessel growth directly or indirectly, which may be correlated with angiopoiesis and development[5]. It is reported that malignant tumor cells can secret plenty of cytokines during tumor development, which lead to rapid proliferation of malignant tumor, and higher expression of TGF-β1[6]. Besides, VEGF is a glycoprotein with many functions. It can stimulate proliferation and migration of vascular endothelial cell. And it is also the strongest vascular permeability agent. It can lead to pathological angiopoiesis, including increasing mitosis and migration of vascular endothelial cell[7,8].

    CT perfusion imaging is a non-invasive method to evaluate growth of microvessels in tumor tissues, then to reflect physiological and metabolic changes via detecting various parameters. Besides, it can also indicate clinical change characters of tumors before any change in size occurs or just when tiny change occurs. Tacelli et al found that APA detected by dynamic contrast-enhance spiral CT is positively correlated with VEGF expression of adenocarcinoma. BF, BV, MTT, TTP and PS can reflect microcirculation characters from various aspects[9]. This study showed that the level of plasma TGF-β1 and VEGF were positively correlated with BF, BV, and MTT, and negatively correlated with TTP. It indicates that TGF-β 1 and VEGF expressions are increased, new vessels are increased and circulation is also increased. From macroscopic aspect, CT

    imaging is characterized as increased blood flow in per unit volume of tumor tissue per unit time (BF), increased blood volume in per unit volume of tumor tissue (BV), and delayed time for contrast agent passing tissue capillary[10]. However, PS which indicates permeability surface and integrity of capillary, shows no significant correlation with TGF-β1 or VEGF. Tumor node metastasis staging reflects tumor progress degree. The higher the stage is, the severer the progress is, the wider the affected tissue. This progress is closely correlated with angiopoiesis. Our study showed the difference in parameters between ⅡB-ⅢA and ⅢB-Ⅳ is more significant thanⅠA -ⅡA. This trend is consistent with TGF-β1 and VEGF levels in plasma. It indicates that CT perfusion imaging not only can be used in diagnosis and differentiation of benign and malignant tumors, but also can be used in NSCLC staging and prognosis.

    In conclusion, a plenty of angiogenesis occurs during tumor development. It can lead to increased TGF-β1 and VEGF levels. CT perfusion imaging can reflect density and structure of microvascular system from imaging aspect. The combination of both is valuable in diagnosis, differentiation, treatment and prognosis.

    Conflicts of interest statement

    The authors have no conflicts of interest.

    [1] Das M, Wakelee H. Angiogenesis and lung cancer: ramucirumab prolongs survival in 2(nd)-line metastatic NSCLC. Transl Lung Cancer Res 2014; 3(6): 397-399.

    [2] Engels EA, Jennings L, Kemp TJ, Chaturvedi AK, Pinto LA, Pfeiffer RM, et al. Circulating TGF-β1 and VEGF and risk of cancer among liver transplant recipients. Cancer Med 2015; 4(8): 1252-1257.

    [3] Lee JE, Kim C, Yang H, Park I, Oh N, Hua S. Novel glycosylated VEGF decoy receptor fusion protein, VEGF-Grab, efficiently suppresses tumor angiogenesis and progression. Mol Cancer Ther 2015; 14(2): 470-479.

    [4] Saini R, Hoyt K. Recent developments in dynamic contrast-enhanced ultrasound imaging of tumor angiogenesis. Imaging Med 2014; 6(1): 41-52.

    [5] Ryzhov SV, Pickup MW, Chytil A, Gorska AE, Zhang QKm Owens P, et al. Role of TGF-β signaling in generation of CD39+CD73+myeloid cells in tumors. J Immunol 2014; 193(6): 3155-3164.

    [6] Kim JW, Koh Y, Kim DW, Ahn YO, Kim TM, Han SW, et al. Clinical implications of VEGF, TGF-β1, and IL-1β in patients with advanced non-small cell lung cancer. Cancer Res Treat 2013; 45(4): 325-333.

    [7] Alevizakos M, Kaltsas S, Syrigos KN. The VEGF pathway in lung cancer. Cancer Chemother Pharmacol 2013; 72(6): 1169-1181.

    [8] Smith NR, Wedge SR, Pommier A, Barry ST. Mechanisms that influence tumour response to VEGF-pathway inhibitors. Biochem Soc Trans 2014; 42(6): 1601-1607.

    [9] Tacelli N, Santangelo T, Scherpereel A, Alain Duhamel, Valérie Deken, Ernst Klotz, et al. Perfusion CT allows prediction of therapy response in non-small cell lung cancer treated with conventional and anti-angiogenic chemotherapy. Eur Radiol 2013; 23(8): 2127-2136.

    [10] Knobloch G, Jost G, Huppertz A, Pietsch H. Dual-energy computed tomography for the assessment of early treatment effects of regorafenib in a preclinical tumor model: comparison with dynamic contrastenhanced CT and conventional contrast-enhanced single-energy CT. Eur Radiol 2014; 24(8): 1896-1905.

    ent heading

    10.1016/j.apjtm.2016.01.009

    *Corresponding author: Yu-Sen Shi, Associate Chief Physician, Radiology Department, Affiliated Hospital of Hainan Medical College, Hainan, Haikou 570102, China.

    E-mail: sunbird709@126.com

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