Overview of Pharmacological&Ecological Research of Scirpus tabernaemontani Gmel

Jiang GONG，Wenjin XU，Yarong MA，Xiaowei ZHANG，Dongdong ZHAO，Jiahua HU，Lei YU，Haiyan HUANG，Shifeng Nl*.Department of Medicine，Tibet Nationality College，Xianyang 708，China；.The College of Life Sciences，Northwest University，Xi’an 70069，China；.Physics Department，Northwest University，Xi’an 70069，China

Overview of Pharmacological&Ecological Research of Scirpus tabernaemontani Gmel

Jiang GONG1，Wenjin XU2，Yarong MA2，Xiaowei ZHANG3，Dongdong ZHAO3，Jiahua HU3，Lei YU3，Haiyan HUANG2，Shifeng Nl2*
1.Department of Medicine，Tibet Nationality College，Xianyang 712082，China；
2.The College of Life Sciences，Northwest University，Xi’an 710069，China；
3.Physics Department，Northwest University，Xi’an 710069，China

On the basis of extensive literature search，the species，ingredients，pharmacology，clinical applications，ecological value and cultivation techniques of Scirpus tabernaemontani Gmel have been reviewed，to provide scientific data for further research&development.

Scirpus tabernaemontani Gmel；Pharmacology；Clinical applications；Ecological value；Cultivation techniques

l ntroduction to Scirpus tabernaemontani Gmel

Scirpus tabernaemontani G-mel is a species of flowering plant in the sedge family Scirpus tabernaemontani genre known by the common names softsterm bulrush and great bulrush［1］.It is a perennial herb producing dense stands of many narrow erect stems reaching 1-2 m，shape like green Chinese onion but much thinner.The stem is in cylindrical shape，hollow inside.It grows from a long rhizome system with many fibrous roots；it can be found in various wetland environment，both at home and abroad［2］.S.validus f."Mosaic"is a variant of Scirpus tabernaemontani，and with yellow-greenish cyclic annular streaks，it has more ornamental value than the original strain［3］.

Components

LIU［4］reported that Scirpus tabernaemontani contained the following components:styrene，camphor，borneol，menthol，styrene ether， isopiperitenone，eucarvone，ethanone，α-aromadendrene，4，7，7α-trimethyl-5，6，7，7α-tetrahydro benzofuranone，fluorene and phenanthrene.

Pharmacological Actions

FAN［5］used ethanol，n-hexane，acetone and butyl alcohol as a solvent to extract the Scirpus tabernaemontani plants，and the inhibitory zone method was used to test the antibacterial effects of the various extracts on seven normal microbials，namely，Escherichia coli，Staphylococcus aureus，Streptococcus mutans，Streptococcus oralis，Bacillus subtilis，Aspergillus and Penicillium.The results showed that Scirpus tabernaemontani extracts could inhibit the growth of seven normal microbial.

Clinical Applications

GU［6-7］reported that the treatment of 60 cases of benign prostatic hyperplasia （BPH）proved that long-term use of Scirpus tabernaemontani could prevent BPH，reduce the residual urine volume，improve lower urinarytract symptoms and maximal urinary flow rate（Qmax）.

Ecological Values

Effects on heavy metals

Accumulation ability of Cd in water body:the heavy metals in the water environment had high toxicity to aquatic animals and plants and hard to remove，and the pollution not only affected the aquatic ecological system but also threated human health［8］. Through accumulation in the body and absorption of the roots system，Scirpus tabernaemontani showed significant effects on the purification of heavy metals in water body［9］.Observations and research have found that Scirpus tabernaemontani had great tolerance to Cd pollution of high levels in the soil，and high rate of bioaccumulation to Cd［10-11］.

Accumulation ability of Pb in water body:Pb is one of the known pollutants of heavy metal with the largest toxicity，and it has strong cumulativity and irreversibility.Scirpus tabernaemontani showed significant effects on the purification of Pb2+in water body through accumulation in the body and absorption of the roots system［12］.

Accumulation ability of Zn in water body:wetland plant Scirpus tabernaemontani was hydroponically cultured with different concentrations of treatment of ZnCl2solutions （0，50，100，300，800，1 500 and 2 500 mg/L），and the results showed that zinc ion concentrations of treatment solutions were different in different parts of Scirpus tabernaemontani［13］.

Removal of mercury in water body:Skinner［14］reported that Scirpus tabernaemontani plants were exposed to concentrations of 0，0.5 or 2 mg/L of mercury for 30 d，and Microtox（water）and cold vapor Atomic Absorption Spectroscopy（AAS0 were used to detect the roots and water.The Microtox results indicated that the mercury induced acute toxicity had been removed from the water，and AAS confirmed an increase of mercury within the plant root tissue and a corresponding decrease of mercury in the water.

Removal of heavy metals in mine drainage:Scirpus tabernaemontani showed good removal effects on the heavy metals（Cu，Zn，Ni，Mn，Cd）in mine drainage，especially good to the removal of Ni，Mn and Cd with the removal rate reaching over 80%.Therefore，Scirpus tabernaemontani is suitable to be used as artificial wetland plant to treat and purify the heavy metals in mine drainage［15-16］.

Effects on organic pollutants

Degradation of pentachlorophenol:pentachlorophenol and its sodium salts were widely used as a fungicide and wood preservatives.However，with stable chemical property，long residual period，high toxicity，broadspectrum toxicity and mutagenicity to organisms，it was listed as the prior monitoring organic pollutant with longlasting effects in the environment.And scientists had attached great importance to its existence and treatment. Scirpus tabernaemontani showed certain accumulation ability to pentachlorophenol.And therefore，it is feasible to use Scirpus tabernaemontani to remediate pentachlorophenol，the hardly biodegradable organic pollutant，in the soil［17-20］.

Phytoremediation of BDE-209: due to its excellent inflaming retarding performances， Decabromodiphenyl ether was widely used as a bromide flaming retardants.In recent years，it found that it had long-lasting effects，which was easy to bioaccumulate and had endocrine disrupting effects. Aquatic macrophyte of Scirpus tabernaemontani Gmel showed good phytoremediation effects on BDE-209 in sediment，which was also one of the most promising remediation techniques to solve the organic pollutants in sediment at present［21］.

Effects on water eutrophication

Water eutrophication and organic pesticides pollution were the main aspects of water pollution.Scirpus tabernaemontani showed certain purification effects on nitrogen，phosphorus and other nutrients in the eutrophic body of water，making it able to be used to carry out the phytoremediation of this kind of water bodies［22-24］.FU［25］reported that Scirpus tabernaemontani showed significant purification effects on the highly salty reuse water in Tianjin-Economic and Technology Development Area Landscape River（short for TEDA），and had significant purification effects on the pollutants of COD，TN，NH3-N，NO3-N，TP，PO4-P in the water.LIU［26］studied the removal efficiency of 6 macrophyte species（Phragmites communis，Typha angustifolia，Acorus calamus，Nymphaea tetragona，Scripus validus，and Pontederia cordata）for nitrogen and phosphorus by growing them in eutrophic ponds.And the results showed that Scripus validus and Acorus calamus showed the highest removal efficiency for nitrogen and phosphorus.Especially Scripus validus by，it was easy to grow and had good adaptability，making it worthy to be applied in water treatment.

Anti-cyanobacterial allelopathic effects

Nakai［27］reported that in order to find out the suitable artificial floating island plant，the methanol extracts of Scirpus were investigated by observing the effects on the growth of Microcystis aeruginosa，and the results showed that the roots of Scirpus contained antimicrobial ingredients，making it a good artificial floating island plant.

Effects on pesticides in water body

Removal of chlorpyrifos:chlorpyrifos was a kind of pesticide，which had large-consumption in Chinese agricultural and high detection rate in water environment.It was of great economic and social values to screen out safe，economic and effective aquatic plants to treat and remediate the pollution of chlorpyrifos to water. Scirpus showed good removal efficiency for chlorpyrifos in water body［28-30］.

Degradation of dinethoate in water:dinethoate was a commonly used phosphorus peticide.Due to the unreasonable application， a lot of dimethoate ran into the surface water with the rainfall runoff without absorption，causing organic pollution.Scirpus could promote the removal of dimethoate in aqueous solution［31］.

Removal of nicosulfuron:LI［32］reported that Scirpus showed certain absorption effects on nicosulfuron in artificial wetland environment，in which the root system played an important part.

Removal of atrazine:WANG［33］found that elevated concentration of atrazine （＞8 mg/L）had significant ef-fects on biomass and physiological characteristics of Scirpus tabernaemontani，decreasing the peroxidase（POD）activity，root activity，chlorophyll content，leaf relative water content and fresh weight，increasing MDA content. However，there was no significant effect in low concentrations.

Cultivation Techniques

Breeding methods

It could achieve the division propagation by using the thizoid stolon.Between the spring equinox and the Qingming Festival，cut open the stolon，first plant in pots or jars，and then transplant to a suitable planting area when the plant grow into the height of 50-60 cm［3］.CHEN［34］used Scirpus tabernaemontani in Northwest Yunnan as materials to establish the tissue culture technology.And the explant disinfection method was as follows:after cold storage for 48 h，the bud was treated with 70%ethanol for 30 s，and then 0.1%HgCl2for 8 min. The best proliferation culture medium formula was:1/2MS+6-BA2.0+IBA 0.02 mg/L，adding 20 g/L of sugar，making the proliferation coefficient reach 12.17.The best rooting medium formula was MS+AC 1 g/L，MS+IBA 0.01 mg/L，making the rooting rate reach 100%，85%，respectively.The survival rate of acclimatization and transplant reached 100%.

Potting Scirpus

Select the pots with no drainage hole，dig the wild roots and cut into pieces before the budding in early spring or after the leaves falling in autumn，put them in the loose soil at the 3/4 of the height，bury the prepared root blocks evenly in the soil at 2-3 cm with enough water，put in the place with strong sunshine，and keep the soil in the pot wet［1］.

Water planting Scirpus

With certain ornamental value，Scirpus tabernaemontani is a suitable kind of aquatic flowers to construct artificial waterscape in residential area［35-36］.

Culture environments

ZHAO［37］studied the growth characteristics and resistance-realted matters （MDA，proline）of the Scirpus tabernaemontani（experimental group）grown in the zero ground water level environment，and compared with the Scirpus tabernaemontani （control group）grown in the normal water environment（with water flooding about 20 cm）to find the effects of ground water level on the growth of Scirpus tabernaemontani when the ground water level changed into zero.The results showed that ground water level changing into zero was unfavorable for the growth of Scirpus tabernaemontani，but reduced the productivity of the wetland ecological system with Scirpus tabernaemontani as the major plants.ZHANG［38］systematically analyzed the planting methods among Iris pseudacorus，Scirpus validus and Acorus calamus with normal planting density but different planting models which were pure plantation，mixed by group plants，and mixed by individual plant.The results showed that when I.pseudacorus and A.calamus were mix-planted with S.validus separately，S.validus would exhibit a faster elongation in the stem to restrain the former two.Studied the optimal soil and water characteristics most suitable for the growth of Scirpus tabernaemontani，including the content of organic matter，soil fertility，conductivity，pH value，soil property，temperature etc.

Summary and Prospect

Scirpus tabernaemontani is an important factor in water scenery designing，and it also can effectively purify and maintain the water quality of the landscape water，making it play an important role in the environment of green remediation because of its beautiful appearance with ornamental value，and because it is an important aquatic flowers.There is pretty little research on the components，pharmacology and clinical application of Scirpus tabernaemontani，and therefore it is worthwhile to strengthen the relevant research.With the promotion of people’s awareness of green environment，Scirpus tabernaemontani will surely be widely applied in ecological environmental protection and medical treatments.

References

［1］ZHAO JG（趙建剛）.Charming pot Scirpus tabernaemontani（迷人的盆栽水蔥）［J］.China Flower&Penjing，2007，（4）: 11-11.

［2］LIU MC（劉慕春）.Scirpus tabernaemontani（水蔥）［J］.Environmental Protection，1975，（3）:38-38.

［3］SHI XL（施雪良），ZHANG JF（張金鋒），ZHU XN（朱興娜）.New excellent aquatic plant variety-Scipus validus f.“Mosaic”（優(yōu)良的水生植物新品種-花葉水蔥）［J］.Modern Landscape Architecture，2006，（2）:64-64.

［4］LIU PY（劉鵬巖），F(xiàn)U CY（傅承尤）.GCMS analysis of volatile oil from Scirpus tabernaemontani（河北水蔥揮發(fā)油的GC-MS分析）［J］.Journal of Instrumental Analysis，1992，11（3）:4-4.

［5］FAN Z（范錚），SUN PL（孫培龍），MA X（馬新），et al.Antibacterial effect of extracts of Onion extract to study the antibacterial effect of Scirpus tabernaemontani（水蔥提取物的抗菌作用研究）［J］.Food Science and Technology，2013，38（2）: 214-217.

［6］GU YN（顧懿寧），LIU CL（劉春林），MIAO AZ （繆愛珠），et al.Curative effect of Scirpus tabernaemontani on the treatment of benign prostatic hyperplasia（水蔥治療良性前列腺增生的療效）［J］. Jiangsu Medical Journal，2013A，39（8）: 974-975.

［7］GU YN（顧懿寧），LIU CL（劉春林），LE KP（樂克平），et al.Single Scirpus tabernaemontani for the treatment of benign prostate hyperplasia（單味水蔥治療良性前列腺增生）［J］.Jiangsu Medical Journal，2013B，11:1354-1354.

［8］NIE L（聶磊），HE MM（賀漫媚）.A study on growth and sewage purification ornamental emerged plants under rivulet water pollution（觀賞挺水植物在河涌污水中的生長及凈化效果研究）［J］.Acta Agriculturae Universitatis Jiangxiensis，2012，34（4）:832-838.

［9］REN J（任珺），TAO L（陶玲），YANG Q（楊倩），et al.Accumulation ability of Cd in water for Phragmites australis，Acorus calamus and Scirpus tabernaemontani（蘆葦、菖蒲和水蔥對水體中Cd富集能力的研究）［J］.Journal of Agro-Environment Science，2010，29（9）:1757-1762.

［10］LI S（李碩）.Remediation potential of Scirpus tabernaemontani to Cd contaminated soil（水蔥對鎘污染土壤修復(fù)潛力的研究）［D］.Hunan University，2006A.

［11］LI S（李碩），LIU YG（劉云國），LI YL（李永麗），et al.Research on the remediation potential of Scirpus tabernaemontani to Cd contaminated soil（水蔥修復(fù)土壤鎘污染潛力的研究）［J］.Environmental Pollution and Control，2006B，28（2）: 84-86.

［12］REN J（任珺），TAO L（陶玲），YANG Q（楊倩）.Accumulation ability of Phragmites australis，Acorus calamus and Scirpus tabernaemontani for Pb2+in water body（蘆葦、菖蒲和水蔥對水體中Pb2+富集能力的研究）［J］.Wetland Sci-ence，2009，7（3）:255-259.

［13］REN J（任珺），F(xiàn)U ZW（付朝文），TAO L（陶玲），et al.Accumulation ability of Phragmites australis，Acorus calamus and Scirpus tabernaemontani for Zn2+in water body（蘆葦、菖蒲和水蔥對水體中Zn2+的富集效應(yīng)研究）［J］.Wetland Science，2011，9（4）:322-326.

［14］SKINNER KATHLEEN，WRIGHT NICOLE，PORTER-GOFF EMILY.Mercury uptake and accumulation by four species of aquatic plants［J］.Environmental Pollution（Barking，Essex:1987），2006，145（1）:234-237.

［15］WANG XG（王新剛），LV XW（呂錫武），WU YF（吳義鋒），et al.Study on advanced treatment of petro-chemical waste water with different aquatic plants（不同水生植物深度凈化石化廢水效果研究）［J］.Safety and Environmental Engineering，2008，15（3）:18-19.

［16］HUANG ZD（黃振東），JING DB（靖德兵），WANG D（王東），et al.The removal effection of Scirpus Validus on heavy metals in mine drainage（水蔥對礦山排水中重金屬的去除研究）［J］. Journal of Capital Normal University（Natural Science Edition），2012，33（5）: 31-36.

［17］XIONG J（熊珺），GAO CX（高創(chuàng)新），YUAN H（袁恒），et al.Phytoremediation of pentachlorophenol-contaminated soil by Scirpus tabernaemontani Gmel（水蔥對五氯酚污染土壤植物修復(fù)的初步研究）［J］.Journal of Agro-Environment Science，2006，25 （6）:1493-1497.

［18］GAO CX（高創(chuàng)新）.Phytoremediation of pentachlorophenol-contaminated soil by aquatic plants（五氯酚污染土壤的水生植物修復(fù)研究）［D］.Huazhong Normal University，2008.

［19］XIONG J（熊珺），F(xiàn)ENG WK（馮偉科），WANG XD（王學(xué)東）.Study on bioaccumulation of pentachlorophenol in different kinds of plants（不同植物對五氯酚生物富集能力的研究）［J］. Guangzhou Chemical Industry，2011，39（7）:44-46.

［20］XIONG J（熊珺）.Residue analysis and phytoremediation of aniline and pentachlorophenol in water environment（苯胺和五氯酚在水環(huán)境中殘留分析及生物修復(fù)的研究）［D］.Huazhong Normal University，2006.

［21］ZHAO LY（趙良元）.Mechanisms of the phytoremediation of decabromodiphenyl ether in sediment by aquatic macrophyte Scirpus validus Vahl（水生植物水蔥對沉積物中十溴聯(lián)苯醚的修復(fù)機(jī)制研究）［D］.Huazhong Normal University，2012.

［22］WU JQ（吳建強(qiáng)），DING L（丁玲）.Study on treatment of polluted river water using pilot-scale surface flow constructed wetlands system（不同植物的表面流人工濕地系統(tǒng)對污染物的去除效果）［J］.Environmental Pollution and Control，2006，28（6）:432-434.

［23］TANG XQ（湯顯強(qiáng)），LI JZ（李金中），LI XJ（李學(xué)菊），et al.Research on seven hydrophytes’removal effect on nitrogen and phosphorus in eutrophic water（7種水生植物對富營養(yǎng)化水體中氮磷去除效果的比較研究）［J］.Journal of Subtropical Resources and Environment，2007，2（2）:8-13.

［24］REN ZY（任照陽）.Study on the emergent remediation technology in eutrophic water bodies of tributaries of the Three Gorges Reservoir（三峽庫區(qū)支流富營養(yǎng)化挺水植物修復(fù)技術(shù)研究）［D］.Chongqing University，2007.

［25］FU CP（付春平），TANG YP（唐運(yùn)平），CHEN XJ（陳錫劍），et al.Effects of purification highly salty reuse water quality by three plants in TEDA landscape river（3種植物對泰達(dá)高鹽再生水景觀河道水質(zhì)的凈化）［J］.Journal of Chongqing University（Natural Science Edition），2006，29（10）:118-120.

［26］LIU CG（劉春光），WANG CS（王春生），LI H（李賀），et al.Removal efficiency for nitrogen and phosphorus in eutrophic waterbody bby macrophytes（幾種大型水生植物對富營養(yǎng)水體中氮和磷的去除效果）［J］.Journal of Agro-Environment Science，2006，25（Suppl）:635-63

［27］NAKAI S，ZOU G，OKUDA T，et al. Anti-cyanobacterial allelopathic effects of plants used for artificial floating islands［J］.Allelopathy Journal，2010，26（1）:113-121.

［28］WANG QH（王慶海），YANG J（陽娟），WU JY（武菊英），et al.Phytoremediation of chlorpyrifos in aquatic environment by three emergent macrophytes（3種挺水植物對水體中毒死蜱去除的過程和效率分析）［J］.Journal of Agro-Environment Science，2010，29（4），: 769-772.

［29］HUANG Y（黃亞）.Study on phytoremediation and organic pesticide polluted water body（植物修復(fù)富營養(yǎng)化及有機(jī)農(nóng)藥污染水體技術(shù)研究）［D］.Tongji U-niversity，2006.

［30］YANG J（陽娟）.Removal ability of chlorpyrifos by some aquatic plants（幾種水生植物對毒死蜱的去除能力）［D］. Huazhong Agricultural University，2008.

［31］FU YG（傅以鋼），HUANG Y（黃亞），ZHANG YL（張亞雷），et al.Effects of three aquatic plants on the degradation of dinethoate in water（3種水生植物對水溶液中樂果的降解作用研究）［J］.Journal of Agro-Environment Science，2006，25（1）:90-94.

［32］LI YM（李詠梅），WEI HL（魏海林）.Experimental study on removal of organic pesticide nicosulfuron by constructed wetland（人工濕地對有機(jī)農(nóng)藥煙嘧磺隆去除的試驗(yàn)）［J］.Journal of Tongji University（Natural Science），2012，40（10）: 1532-15351547.

［33］Wang Qing-Hai，Zhang Wei，Que Xiao-E，et al.Effects of atrazine residue in water on biomass and physiological characteristics of Scirpus tabernaemontani［J］.Chinese Journal of Plant Ecology，2011，35（2）:223-231.

［34］CHEN ZY（陳澤英）.Tissue culture of seven kinds of aquatic plants in Northwest Yunnan（滇西北七種水生植物的組織培養(yǎng)研究）［D］.Southwest Forestry University，2011.

［35］LIU XF（劉旭富）.Study on the adaptability of several common aquatic flowers of life water（幾種常見水生花卉對生活中水的適應(yīng)性研究）［J］.Journal of Beijing Agricultural Vocation College，2008，22（3）:32-34.

［36］LIU Y（劉艷），LI DL（李冬玲），REN QJ（任全進(jìn)）.Appreciation and application of several kinds of new excellent aquatic flowers（幾種新優(yōu)水生花卉的觀賞和利用）［J］.Chinese Wild Plant Resources，2004，23（3）:26-27.

［37］ZHAO XJ（趙湘江），TIAN K（田昆），YUE HT （岳海濤）.Effects of water level changes on the growth stress of plateau wetland lakeside dominant plants Scirpus（水位變化對高原濕地湖濱帶優(yōu)勢植物水蔥的生長脅迫）［J］，Guangxi plant，2014，11:1-9.

［38］ZHANG Q（張群），ZHU Y（朱義），CUI XH（崔心紅）.Effects of different planting methods on the growth of Iris pseudacorus，Scirpus validus and Acorus calamus（不同種植方式對黃菖蒲、水蔥和菖蒲3種挺水植物生長的影響）［J］.China Agricultural Science Bulletin，2014，30（28）:210-216.

Responsible editor:Na LI

Responsible proofreader:Xiaoyan WU

水蔥藥學(xué)及生態(tài)價(jià)值研究概況

鞏江1，徐文進(jìn)2，馬亞榮2，張小偉3，趙冬冬3，胡家華3，宇磊3，黃海艷2，倪士峰2*（1.西藏民族學(xué)院醫(yī)學(xué)院，陜西咸陽 712082；2西北大學(xué)生命科學(xué)學(xué)院，陜西西安 710069；3.西北大學(xué)物理學(xué)院，陜西西安 710069）

水蔥是重要的水體景觀要素，能有效地凈化和維持景觀水體的水質(zhì)，在環(huán)境綠色修復(fù)方面發(fā)揮重要作用。有關(guān)其成分、藥理和臨床用途的研究報(bào)道目前還比較少。在廣泛文獻(xiàn)檢索的基礎(chǔ)上，對水蔥種屬、成分、藥理作用、臨床應(yīng)用、生態(tài)價(jià)值和栽培技術(shù)等進(jìn)行了概述，為深入研究和開發(fā)利用提供科學(xué)資料。

水蔥；藥理作用；臨床應(yīng)用；生態(tài)價(jià)值；栽培技術(shù)

西部資源生物與現(xiàn)代生物技術(shù)教育部重點(diǎn)實(shí)驗(yàn)室基金（KH09030）；西藏自治區(qū)科技廳重大科技專項(xiàng)基金（20091012）；陜西省教育廳科學(xué)研究項(xiàng)目計(jì)劃（2010JK862）。

鞏江（1975-），女，甘肅蘭州人，高級實(shí)驗(yàn)師，碩士；研究方向：民族藥化學(xué)與資源學(xué)。*通訊作者，男，江蘇沛縣人，副研究員，博士，碩士研究生導(dǎo)師，研究方向：中藥化學(xué)與資源學(xué)，E-mail：nsfstone@126.com。。

2015-00-00

2015-00-00

Supported by Opening Foundation of Key Laboratory of Resource Biology and Biotechnology in Western China （Northwest University），Ministry of Education（KH09030）；the Major Scientific and Technological Project of the Department of Science and Technology，Tibet Autonomous Region （20091012）；the Scientific Research Plan Projects of Shaanxi Education Department（2010JK862）.

.E-mail:nsfstone@126.com

December 30，2014Accepted:March 5，2015