212007 A study on the relationship between HER-2 gene amplification and protein expression in invasive breast cancer

/Chen Shunping(陳順平，Dept Pathol，F(xiàn)uzhou Gen Hosp Nanjing Mil Command，F(xiàn)uzhou 350025)…∥Chin J Gen Surg.-2011，86(10).-829～832

ObjectiveTo study the amplification and expression of HER-2 gene and protein in breast cancer and to investigate the relationship between HER-2 gene and Ki-67，P53，ER，PR，lymph node metastasis and TNM staging.MethodsFluorescence in situ hybridization(FISH)and immunohistochemistry(IHC)method were used to detect the amplification and expression of HER-2 and the expression of Ki-67，P53，ER，PR in 150 cases of breast cancer.Results49 out of 150 cases were amplificated，positive rate of HER-2 gene was 32.68%.In the 32 patients whose expression of HER-2 protein was(+++)，HER-2 gene was expressed in 28 cases.In the 46 patients whose expression of HER-2 protein was(++)，HER-2 was expressed in 16 cases.In the 26 patients whose expression of HER-2 protein was(+)，HER-2 was expressed in 3 cases.In the 47 cases that the expression of HER-2 protein was negative，there were 45 cases that the expression HER-2 gene was negative.The expression of HER-2 was correlated with ER，PR expression and TNM staging，but not significantly related with age，Ki-67，P53 and lymph node metastasis.ConclusionFISH and IHC correlate well with each other in the determination of HER-2 gene amplication and protein expression in cases of invasive breast cancer.11 refs，7 figs，1 tab.